Q08078 (CP2CP_MESAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 2C25
EC=1.14.14.1

Alternative name(s):
CYPIIC25
Cytochrome P450 HSM1

Gene names

Name:

CYP2C25

Organism

Mesocricetus auratus (Golden hamster)

Taxonomic identifier

10036 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Mesocricetus

Protein attributes

Sequence length	490 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the hydroxylation of tolbutamide and the N-demethylation of aminopyrine and benzphetamine. Also has testosterone hydroxylase (16 beta) activity.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Induction	P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 490	490	Cytochrome P450 2C25		PRO_0000051713
Sites
	Metal binding	435	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q08078 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: AB040B2ECAB80453	FASTA	490	55,967
	10 20 30 40 50 60 MDAVLVLVFI LSSLVFLSLW RQSSERRKLP PGPTPLPIIG NFLQIDVKNI SGSLTNFSKV 70 80 90 100 110 120 YGPVFTLYLG MKPTVVLHGY ETVKEALIDH GEEFAGRGDF PMAERINKGL GIVFSNGNRW 130 140 150 160 170 180 KEIRRFSLMT LRNLGMGKRN IEDRVQEEAQ CLVEELRKTN GSPCDPTFIL SCAPCNVICS 190 200 210 220 230 240 IIFQNRFDYK DQDFLTFMKK VNENVRILSS PWLQVCNNFP SLIDYCPGSH HKITKNVNYL 250 260 270 280 290 300 KKYILEKIEE HQESLDIENP RDFIDYYLIK LKQANHNQQS EFSLENLTTT VSDLFGAGTE 310 320 330 340 350 360 TTSTTLRYAL LLLLKHPHVT AKVQEEIDQV VGRHRKPCMQ DRSHMPYTDA MIHEVQRFID 370 380 390 400 410 420 LIPISLPHAV TCDIKFRDYF IPKGTTVITS LSSVLHDNKE FPNPEVFDPG HFLDKNGNFK 430 440 450 460 470 480 KSDYFMPFSA GKRICAGEGL ARMELFLFLT TILQNFKLKS MIHPKDIDTT PVVNGFASLP 490 PSYQLCFIPV

References

[1]

"Sex-related differences in the expression of cytochrome P450 in hamsters: cDNA cloning and examination of the expression of three distinct CYP2C cDNAs." Sakuma T., Masaki K., Itoh S., Yokoi T., Kamataki T. Mol. Pharmacol. 45:228-236(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X63022 mRNA. Translation: CAA44753.1.
PIR	I48189.
3D structure databases
ProteinModelPortal	Q08078.
SMR	Q08078. Positions 30-490.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG015789.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP2CP_MESAU

Accession

Primary (citable) accession number: Q08078

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program