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Q08062

- MDHC_MAIZE

UniProt

Q08062 - MDHC_MAIZE

Protein

Malate dehydrogenase, cytoplasmic

Gene
N/A
Organism
Zea mays (Maize)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 2 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    (S)-malate + NAD+ = oxaloacetate + NADH.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei93 – 931SubstratePROSITE-ProRule annotation
    Binding sitei99 – 991SubstratePROSITE-ProRule annotation
    Binding sitei106 – 1061NADBy similarity
    Binding sitei113 – 1131NADBy similarity
    Binding sitei132 – 1321SubstratePROSITE-ProRule annotation
    Binding sitei163 – 1631SubstratePROSITE-ProRule annotation
    Active sitei188 – 1881Proton acceptorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi12 – 187NADBy similarity
    Nucleotide bindingi130 – 1323NADBy similarity

    GO - Molecular functioni

    1. L-malate dehydrogenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellular carbohydrate metabolic process Source: InterPro
    2. malate metabolic process Source: InterPro
    3. response to cadmium ion Source: EnsemblPlants/Gramene
    4. response to salt stress Source: EnsemblPlants/Gramene
    5. response to zinc ion Source: EnsemblPlants/Gramene
    6. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    NAD

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate dehydrogenase, cytoplasmic (EC:1.1.1.37)
    OrganismiZea mays (Maize)
    Taxonomic identifieri4577 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

    Organism-specific databases

    GrameneiQ08062.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. apoplast Source: EnsemblPlants/Gramene
    2. chloroplast stroma Source: EnsemblPlants/Gramene
    3. cytosol Source: EnsemblPlants/Gramene
    4. nucleus Source: EnsemblPlants/Gramene
    5. plasma membrane Source: EnsemblPlants/Gramene
    6. plasmodesma Source: EnsemblPlants/Gramene
    7. vacuolar membrane Source: EnsemblPlants/Gramene

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 332332Malate dehydrogenase, cytoplasmicPRO_0000113415Add
    BLAST

    Proteomic databases

    PRIDEiQ08062.
    ProMEXiQ08062.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ08062.
    SMRiQ08062. Positions 4-331.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. MDH type 2 family.Curated

    Phylogenomic databases

    HOGENOMiHOG000220953.
    KOiK00025.
    OMAiNCLIASK.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProiIPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR011274. Malate_DH_NAD-dep_euk.
    IPR010945. Malate_DH_type2.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR23382. PTHR23382. 1 hit.
    PfamiPF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMiSSF56327. SSF56327. 1 hit.
    TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
    TIGR01758. MDH_euk_cyt. 1 hit.
    PROSITEiPS00068. MDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q08062-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKEPMRVLV TGAAGQIGYA LVPMIARGVM LGADQPVILH MLDIPPAAEA    50
    LNGVKMELVD AAFPLLKGVV ATTDVVEACT GVNVAVMVGG FPRKEGMERK 100
    DVMSKNVSIY KSQASALEAH AAPNCKVLVV ANPANTNALI LKEFAPSIPE 150
    KNVTCLTRLD HNRALGQISE RLNVQVSDVK NVIIWGNHSS SQYPDVNHAT 200
    VKTSTGEKPV RELVSDDEWL NGEFITTVQQ RGAAIIKARK FSSALSAASS 250
    ACDHIRDWVL GTPEGTFVSM GVYSDGSYGV PSGLIYSFPV TCSGGEWKIV 300
    QGLPIDEFSR KKMDATAQEL TEEKTLAYSC LE 332
    Length:332
    Mass (Da):35,590
    Last modified:November 1, 1998 - v2
    Checksum:i05D82B33FF0CE26F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF007581 mRNA. Translation: AAB64290.1.
    M95069 mRNA. Translation: AAA18556.2.
    PIRiT02935.
    T03650.
    RefSeqiNP_001105603.1. NM_001112133.1.
    UniGeneiZm.103385.
    Zm.82624.

    Genome annotation databases

    GeneIDi542598.
    KEGGizma:542598.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF007581 mRNA. Translation: AAB64290.1 .
    M95069 mRNA. Translation: AAA18556.2 .
    PIRi T02935.
    T03650.
    RefSeqi NP_001105603.1. NM_001112133.1.
    UniGenei Zm.103385.
    Zm.82624.

    3D structure databases

    ProteinModelPortali Q08062.
    SMRi Q08062. Positions 4-331.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q08062.
    ProMEXi Q08062.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 542598.
    KEGGi zma:542598.

    Organism-specific databases

    Gramenei Q08062.

    Phylogenomic databases

    HOGENOMi HOG000220953.
    KOi K00025.
    OMAi NCLIASK.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProi IPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR011274. Malate_DH_NAD-dep_euk.
    IPR010945. Malate_DH_type2.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR23382. PTHR23382. 1 hit.
    Pfami PF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMi SSF56327. SSF56327. 1 hit.
    TIGRFAMsi TIGR01759. MalateDH-SF1. 1 hit.
    TIGR01758. MDH_euk_cyt. 1 hit.
    PROSITEi PS00068. MDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of cytoplasmic malate dehydrogenase over-expressed in hybrid maize."
      Hu J., Zhao X., Yuan Z., Qian X., Yang J.
      Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Yedan 4.
      Tissue: Leaf.
    2. "Partial sequence analysis of 130 randomly selected maize cDNA clones."
      Keith C.S., Hoang D.O., Barrett B.M., Feigelman B., Nelson M.C., Thai H., Baysdorfer C.
      Plant Physiol. 101:329-332(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-73.
      Tissue: Leaf and Sheath.

    Entry informationi

    Entry nameiMDHC_MAIZE
    AccessioniPrimary (citable) accession number: Q08062
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 110 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3