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Protein

Malate dehydrogenase, cytoplasmic

Gene
N/A
Organism
Zea mays (Maize)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Malate dehydrogenase; catalyzes a reversible NAD-dependent dehydrogenase reaction involved in central metabolism and redox homeostasis.1 Publication

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.1 Publication

Kineticsi

  1. KM=25 µM for NADH for the native enzyme1 Publication
  2. KM=29 µM for NADH for the recombinant enzyme1 Publication
  3. KM=126 µM for oxaloacetate for the native enzyme1 Publication
  4. KM=289 µM for oxaloacetate for the recombinant enzyme1 Publication
  5. KM=1810 µM for malate for the native enzyme1 Publication
  6. KM=266 µM for malate for the recombinant enzyme1 Publication

    pH dependencei

    Optimum pH is 7.41 for the native enzyme.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei93SubstratePROSITE-ProRule annotation1
    Binding sitei99SubstratePROSITE-ProRule annotation1
    Binding sitei106NADBy similarity1
    Binding sitei113NADBy similarity1
    Binding sitei132SubstratePROSITE-ProRule annotation1
    Binding sitei163SubstratePROSITE-ProRule annotation1
    Active sitei188Proton acceptorBy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi12 – 18NADBy similarity7
    Nucleotide bindingi130 – 132NADBy similarity3

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionOxidoreductase
    Biological processTricarboxylic acid cycle
    LigandNAD

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate dehydrogenase, cytoplasmic1 Publication (EC:1.1.1.371 Publication)
    OrganismiZea mays (Maize)
    Taxonomic identifieri4577 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogonodaeAndropogoneaeTripsacinaeZea
    Proteomesi
    • UP000007305 Componenti: Chromosome 1

    Subcellular locationi

    • Cell membrane 1 Publication; Peripheral membrane protein 1 Publication

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001134151 – 332Malate dehydrogenase, cytoplasmicAdd BLAST332

    Proteomic databases

    PaxDbiB2ZAF9.
    C4IZW9.
    Q08062.
    PRIDEiQ08062.
    ProMEXiQ08062.

    Expressioni

    Tissue specificityi

    Expressed constitutively in roots.1 Publication

    Gene expression databases

    ExpressionAtlasiQ08062. baseline.
    GenevisibleiQ08062. ZM.

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    STRINGi4577.GRMZM2G415359_P02.

    Structurei

    3D structure databases

    ProteinModelPortaliQ08062.
    SMRiQ08062.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. MDH type 2 family.Curated

    Phylogenomic databases

    eggNOGiKOG1496. Eukaryota.
    COG0039. LUCA.
    HOGENOMiHOG000220953.
    KOiK00025.
    OMAiQYPDAGQ.
    OrthoDBiEOG09360G1U.

    Family and domain databases

    CDDicd01336. MDH_cytoplasmic_cytosolic. 1 hit.
    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProiView protein in InterPro
    IPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR011274. Malate_DH_NAD-dep_euk.
    IPR010945. Malate_DH_type2.
    IPR016040. NAD(P)-bd_dom.
    PANTHERiPTHR23382. PTHR23382. 1 hit.
    PfamiView protein in Pfam
    PF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMiSSF51735. SSF51735. 1 hit.
    SSF56327. SSF56327. 1 hit.
    TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
    TIGR01758. MDH_euk_cyt. 1 hit.
    PROSITEiView protein in PROSITE
    PS00068. MDH. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q08062-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MAKEPMRVLV TGAAGQIGYA LVPMIARGVM LGADQPVILH MLDIPPAAEA
    60 70 80 90 100
    LNGVKMELVD AAFPLLKGVV ATTDVVEACT GVNVAVMVGG FPRKEGMERK
    110 120 130 140 150
    DVMSKNVSIY KSQASALEAH AAPNCKVLVV ANPANTNALI LKEFAPSIPE
    160 170 180 190 200
    KNVTCLTRLD HNRALGQISE RLNVQVSDVK NVIIWGNHSS SQYPDVNHAT
    210 220 230 240 250
    VKTSTGEKPV RELVSDDEWL NGEFITTVQQ RGAAIIKARK FSSALSAASS
    260 270 280 290 300
    ACDHIRDWVL GTPEGTFVSM GVYSDGSYGV PSGLIYSFPV TCSGGEWKIV
    310 320 330
    QGLPIDEFSR KKMDATAQEL TEEKTLAYSC LE
    Length:332
    Mass (Da):35,590
    Last modified:November 1, 1998 - v2
    Checksum:i05D82B33FF0CE26F
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti202K → E in ACD02021 (Ref. 2) Curated1
    Sequence conflicti254H → Y in ACD02021 (Ref. 2) Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF007581 mRNA. Translation: AAB64290.1.
    EU625276 mRNA. Translation: ACD02021.1.
    BT084116 mRNA. Translation: ACR34469.1.
    M95069 mRNA. Translation: AAA18556.2.
    PIRiT02935.
    T03650.
    RefSeqiNP_001105603.1. NM_001112133.2.
    UniGeneiZm.103385.
    Zm.82624.

    Genome annotation databases

    EnsemblPlantsiZm00001d032695_T001; Zm00001d032695_T001; Zm00001d032695.
    GeneIDi542598.
    GrameneiZm00001d032695_T001; Zm00001d032695_T001; Zm00001d032695.
    KEGGizma:542598.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF007581 mRNA. Translation: AAB64290.1.
    EU625276 mRNA. Translation: ACD02021.1.
    BT084116 mRNA. Translation: ACR34469.1.
    M95069 mRNA. Translation: AAA18556.2.
    PIRiT02935.
    T03650.
    RefSeqiNP_001105603.1. NM_001112133.2.
    UniGeneiZm.103385.
    Zm.82624.

    3D structure databases

    ProteinModelPortaliQ08062.
    SMRiQ08062.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi4577.GRMZM2G415359_P02.

    Proteomic databases

    PaxDbiB2ZAF9.
    C4IZW9.
    Q08062.
    PRIDEiQ08062.
    ProMEXiQ08062.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiZm00001d032695_T001; Zm00001d032695_T001; Zm00001d032695.
    GeneIDi542598.
    GrameneiZm00001d032695_T001; Zm00001d032695_T001; Zm00001d032695.
    KEGGizma:542598.

    Phylogenomic databases

    eggNOGiKOG1496. Eukaryota.
    COG0039. LUCA.
    HOGENOMiHOG000220953.
    KOiK00025.
    OMAiQYPDAGQ.
    OrthoDBiEOG09360G1U.

    Gene expression databases

    ExpressionAtlasiQ08062. baseline.
    GenevisibleiQ08062. ZM.

    Family and domain databases

    CDDicd01336. MDH_cytoplasmic_cytosolic. 1 hit.
    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProiView protein in InterPro
    IPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR011274. Malate_DH_NAD-dep_euk.
    IPR010945. Malate_DH_type2.
    IPR016040. NAD(P)-bd_dom.
    PANTHERiPTHR23382. PTHR23382. 1 hit.
    PfamiView protein in Pfam
    PF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMiSSF51735. SSF51735. 1 hit.
    SSF56327. SSF56327. 1 hit.
    TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
    TIGR01758. MDH_euk_cyt. 1 hit.
    PROSITEiView protein in PROSITE
    PS00068. MDH. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiMDHC_MAIZE
    AccessioniPrimary (citable) accession number: Q08062
    Secondary accession number(s): B2ZAF9, C4IZW9
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: November 1, 1998
    Last modified: March 15, 2017
    This is version 132 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.