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Reviewed, UniProtKB/Swiss-Prot Q08062 (MDHC_MAIZE)

Last modified November 25, 2008. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Malate dehydrogenase, cytoplasmic
    EC=1.1.1.37
OrganismZea mays (Maize)
Taxonomic identifier4577 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACCAD clade › Panicoideae › Andropogoneae › Zea

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Protein attributes

Sequence length332 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Catalytic activity

(S)-malate + NAD(+) = oxaloacetate + NADH.

Subunit structure

Homodimer By similarity.

Subcellular location

CytoplasmBy similarity.

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 2 family.

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Ontologies

Keywords

   Biological processTricarboxylic acid cycle
   Cellular componentCytoplasm
   LigandNAD
   Molecular functionOxidoreductase

Gene Ontology (GO)

   Biological processglycolysis

Inferred from electronic annotation. Source: InterPro

malate metabolic process

Inferred from electronic annotation. Source: InterPro

oxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionL-malate dehydrogenase activity

Inferred from electronic annotation. Source: InterPro

binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 332332Malate dehydrogenase, cytoplasmic
PRO_0000113415

Regions

Nucleotide binding12 – 187NAD By similarity
Nucleotide binding130 – 1323NAD By similarity

Sites

Active site1881Proton acceptor By similarity
Binding site931Substrate By similarity
Binding site991Substrate By similarity
Binding site1061NAD By similarity
Binding site1131NAD By similarity
Binding site1321Substrate By similarity
Binding site1631Substrate By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q08062-1 [UniParc].

Last modified November 1, 1998. Version 2.
Checksum: 05D82B33FF0CE26F

FASTA33235,590
        10         20         30         40         50         60 
MAKEPMRVLV TGAAGQIGYA LVPMIARGVM LGADQPVILH MLDIPPAAEA LNGVKMELVD 

        70         80         90        100        110        120 
AAFPLLKGVV ATTDVVEACT GVNVAVMVGG FPRKEGMERK DVMSKNVSIY KSQASALEAH 

       130        140        150        160        170        180 
AAPNCKVLVV ANPANTNALI LKEFAPSIPE KNVTCLTRLD HNRALGQISE RLNVQVSDVK 

       190        200        210        220        230        240 
NVIIWGNHSS SQYPDVNHAT VKTSTGEKPV RELVSDDEWL NGEFITTVQQ RGAAIIKARK 

       250        260        270        280        290        300 
FSSALSAASS ACDHIRDWVL GTPEGTFVSM GVYSDGSYGV PSGLIYSFPV TCSGGEWKIV 

       310        320        330 
QGLPIDEFSR KKMDATAQEL TEEKTLAYSC LE

« Hide

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References

[1]"Cloning and characterization of cytoplasmic malate dehydrogenase over-expressed in hybrid maize."
Hu J., Zhao X., Yuan Z., Qian X., Yang J.
Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Yedan 4.
Tissue: Leaf.
[2]"Partial sequence analysis of 130 randomly selected maize cDNA clones."
Keith C.S., Hoang D.O., Barrett B.M., Feigelman B., Nelson M.C., Thai H., Baysdorfer C.
Plant Physiol. 101:329-332(1993) [PubMed: 8278499] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-73.
Tissue: Leaf and Sheath.

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Cross-references

Sequence databases

AF007581 mRNA. Translation: AAB64290.1.
M95069 mRNA. Translation: AAA18556.2.
PIRT02935.
T03650.
RefSeqNP_001105603.1.
UniGeneZm.82624
Zm.94996

3D structure databases

HSSPHSSP built from PDB template 4MDH based on UniProtKB P11708.
ModBaseSearch...

Genome annotation databases

GeneID542598.

Organism-specific databases

GrameneQ08062.

Family and domain databases

InterProIPR001557. L-lactate/malate_DHase.
IPR001236. Lactate/malate_DHase.
IPR015955. Lactate_DHase/Glyco_Ohase_4_C.
IPR001252. Malate_DHase_AS.
IPR011274. Malate_DHase_NAD-dep_euk.
IPR010945. Malate_DHase_SF1.
IPR016040. NAD(P)-bd.
[Graphical view]
Gene3DG3DSA:3.90.110.10. lact_mal_DH. 1 hit.
G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHERPTHR23382. MDH_SF1. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
ProDomPD003052. Mal_dehydrog. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsTIGR01759. MalateDH-SF1. 1 hit.
TIGR01758. MDH_euk_cyt. 1 hit.
PROSITEPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameMDHC_MAIZE
AccessionPrimary (citable) accession number: Q08062
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1998
Last modified: November 25, 2008
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents