Forkhead box protein M1 - Homo sapiens (Human)

Names and origin

Protein names

Recommended name:
    Forkhead box protein M1
Alternative name(s):
    Forkhead-related protein FKHL16
    Hepatocyte nuclear factor 3 forkhead homolog 11
      Short name=HNF-3/fork-head homolog 11
      Short name=HFH-11
    Winged-helix factor from INS-1 cells
    M-phase phosphoprotein 2
    MPM-2 reactive phosphoprotein 2
    Transcription factor Trident

Gene names

Name:	FOXM1
Synonyms:	FKHL16, HFH11, MPP2, WIN

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	763 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Transcriptional activatory factor. May play a role in the control of cell proliferation.
Subcellular location	Nucleus.
Tissue specificity	Expressed in thymus, testis, small intestine, colon followed by ovary. Appears to be expressed only in adult organs containing proliferating/cycling cells or in response to growth factors. Also expressed in epithelial cell lines derived from tumors. Not expressed in resting cells.
Developmental stage	Embryonic expression pattern: liver, lung, intestine, kidney, urinary tract; adult expression pattern: intestine, colon, testis and thymus.
Induction	Induced during liver regeneration and oxidative stress.
Domain	Within the protein there is a domain which acts as a transcriptional activator. Insertion of a splicing sequence within it inactivates this transcriptional activity, as it is the case for isoform 4.
Post-translational modification	Phosphorylated in M (mitotic) phase.
Sequence similarities	Contains 1 fork-head DNA-binding domain.

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Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from electronic annotation. Source: InterPro
Molecular function	protein binding Inferred from physical interaction. Source: IntAct sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
E7	P03129	2	EBI-866499,EBI-866453	From a different organism.
FOXO3	O43524	1	EBI-866480,EBI-1644164

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Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select] Notes: Isoform 1 and isoform 2 appear to be the only activators of gene transcription. Isoform 3, found in rat, does not seem to exist in human.

Isoform 1 (identifier: Q08050-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q08050-2) The sequence of this isoform differs from the canonical sequence as follows: 326-340: Missing.
Notes: Highly expressed in testis.

Isoform 4 (identifier: Q08050-3) The sequence of this isoform differs from the canonical sequence as follows: 423-423: V → FGEQVVFGYMSKFFSGDLRDFGTPITSLFNFIFLCLSV

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 763

763

Forkhead box protein M1

PRO_0000091863

Regions

DNA binding

235 – 327

93

Fork-head

Compositional bias

480 – 513

34

Glu/Pro/Ser/Thr-rich

Amino acid modifications

Modified residue

331

1

Phosphoserine

Modified residue

620

1

Phosphothreonine

Modified residue

627

1

Phosphothreonine

Modified residue

662

1

Phosphothreonine Probable

Modified residue

704

1

Phosphoserine

Natural variations

Alternative sequence

326 – 340

15

Missing in isoform 2.

VSP_001547

Alternative sequence

423

1

V → FGEQVVFGYMSKFFSGDLRD FGTPITSLFNFIFLCLSV in isoform 4.

VSP_001548

Natural variant

402

1

A → E

VAR_025239

Natural variant

450

1

F → L

VAR_025240

Natural variant

643

1

S → P: dbSNP rs3742076.

VAR_020024

Natural variant

669

1

P → R

VAR_025241

Natural variant

673

1

P → L

VAR_025242

Experimental info

Sequence conflict

3

1

T → A in AAC63595. Ref.2

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Sequences

Sequence		Length	Mass (Da)
Isoform 1 [UniParc]. Last modified December 1, 2000. Version 3. Checksum: 963CAC8FE7498E9B Show »	FASTA	763	84,283
10 20 30 40 50 60 MKTSPRRPLI LKRRRLPLPV QNAPSETSEE EPKRSPAQQE SNQAEASKEV AESNSCKFPA 70 80 90 100 110 120 GIKIINHPTM PNTQVVAIPN NANIHSIITA LTAKGKESGS SGPNKFILIS CGGAPTQPPG 130 140 150 160 170 180 LRPQTQTSYD AKRTEVTLET LGPKPAARDV NLPRPPGALC EQKRETCADG EAAGCTINNS 190 200 210 220 230 240 LSNIQWLRKM SSDGLGSRSI KQEMEEKENC HLEQRQVKVE EPSRPSASWQ NSVSERPPYS 250 260 270 280 290 300 YMAMIQFAIN STERKRMTLK DIYTWIEDHF PYFKHIAKPG WKNSIRHNLS LHDMFVRETS 310 320 330 340 350 360 ANGKVSFWTI HPSANRYLTL DQVFKPLDPG SPQLPEHLES QQKRPNPELR RNMTIKTELP 370 380 390 400 410 420 LGARRKMKPL LPRVSSYLVP IQFPVNQSLV LQPSVKVPLP LAASLMSSEL ARHSKRVRIA 430 440 450 460 470 480 PKVLLAEEGI APLSSAGPGK EEKLLFGEGF SPLLPVQTIK EEEIQPGEEM PHLARPIKVE 490 500 510 520 530 540 SPPLEEWPSP APSFKEESSH SWEDSSQSPT PRPKKSYSGL RSPTRCVSEM LVIQHRERRE 550 560 570 580 590 600 RSRSRRKQHL LPPCVDEPEL LFSEGPSTSR WAAELPFPAD SSDPASQLSY SQEVGGPFKT 610 620 630 640 650 660 PIKETLPISS TPSKSVLPRT PESWRLTPPA KVGGLDFSPV QTSQGASDPL PDPLGLMDLS 670 680 690 700 710 720 TTPLQSAPPL ESPQRLLSSE PLDLISVPFG NSSPSDIDVP KPGSPEPQVS GLAANRSLTE 730 740 750 760 GLVLDTMNDS LSKILLDISF PGLDEDPLGP DNINWSQFIP ELQ « Hide
Isoform 2 [UniParc]. Checksum: 3224A22B1434A531 Show »		748	82,685
10 20 30 40 50 60 MKTSPRRPLI LKRRRLPLPV QNAPSETSEE EPKRSPAQQE SNQAEASKEV AESNSCKFPA 70 80 90 100 110 120 GIKIINHPTM PNTQVVAIPN NANIHSIITA LTAKGKESGS SGPNKFILIS CGGAPTQPPG 130 140 150 160 170 180 LRPQTQTSYD AKRTEVTLET LGPKPAARDV NLPRPPGALC EQKRETCADG EAAGCTINNS 190 200 210 220 230 240 LSNIQWLRKM SSDGLGSRSI KQEMEEKENC HLEQRQVKVE EPSRPSASWQ NSVSERPPYS 250 260 270 280 290 300 YMAMIQFAIN STERKRMTLK DIYTWIEDHF PYFKHIAKPG WKNSIRHNLS LHDMFVRETS 310 320 330 340 350 360 ANGKVSFWTI HPSANRYLTL DQVFKQQKRP NPELRRNMTI KTELPLGARR KMKPLLPRVS 370 380 390 400 410 420 SYLVPIQFPV NQSLVLQPSV KVPLPLAASL MSSELARHSK RVRIAPKVLL AEEGIAPLSS 430 440 450 460 470 480 AGPGKEEKLL FGEGFSPLLP VQTIKEEEIQ PGEEMPHLAR PIKVESPPLE EWPSPAPSFK 490 500 510 520 530 540 EESSHSWEDS SQSPTPRPKK SYSGLRSPTR CVSEMLVIQH RERRERSRSR RKQHLLPPCV 550 560 570 580 590 600 DEPELLFSEG PSTSRWAAEL PFPADSSDPA SQLSYSQEVG GPFKTPIKET LPISSTPSKS 610 620 630 640 650 660 VLPRTPESWR LTPPAKVGGL DFSPVQTSQG ASDPLPDPLG LMDLSTTPLQ SAPPLESPQR 670 680 690 700 710 720 LLSSEPLDLI SVPFGNSSPS DIDVPKPGSP EPQVSGLAAN RSLTEGLVLD TMNDSLSKIL 730 740 LDISFPGLDE DPLGPDNINW SQFIPELQ « Hide
Isoform 4 [UniParc]. Checksum: A8B8DBA3BDA377EA Show »		800	88,497
10 20 30 40 50 60 MKTSPRRPLI LKRRRLPLPV QNAPSETSEE EPKRSPAQQE SNQAEASKEV AESNSCKFPA 70 80 90 100 110 120 GIKIINHPTM PNTQVVAIPN NANIHSIITA LTAKGKESGS SGPNKFILIS CGGAPTQPPG 130 140 150 160 170 180 LRPQTQTSYD AKRTEVTLET LGPKPAARDV NLPRPPGALC EQKRETCADG EAAGCTINNS 190 200 210 220 230 240 LSNIQWLRKM SSDGLGSRSI KQEMEEKENC HLEQRQVKVE EPSRPSASWQ NSVSERPPYS 250 260 270 280 290 300 YMAMIQFAIN STERKRMTLK DIYTWIEDHF PYFKHIAKPG WKNSIRHNLS LHDMFVRETS 310 320 330 340 350 360 ANGKVSFWTI HPSANRYLTL DQVFKPLDPG SPQLPEHLES QQKRPNPELR RNMTIKTELP 370 380 390 400 410 420 LGARRKMKPL LPRVSSYLVP IQFPVNQSLV LQPSVKVPLP LAASLMSSEL ARHSKRVRIA 430 440 450 460 470 480 PKFGEQVVFG YMSKFFSGDL RDFGTPITSL FNFIFLCLSV LLAEEGIAPL SSAGPGKEEK 490 500 510 520 530 540 LLFGEGFSPL LPVQTIKEEE IQPGEEMPHL ARPIKVESPP LEEWPSPAPS FKEESSHSWE 550 560 570 580 590 600 DSSQSPTPRP KKSYSGLRSP TRCVSEMLVI QHRERRERSR SRRKQHLLPP CVDEPELLFS 610 620 630 640 650 660 EGPSTSRWAA ELPFPADSSD PASQLSYSQE VGGPFKTPIK ETLPISSTPS KSVLPRTPES 670 680 690 700 710 720 WRLTPPAKVG GLDFSPVQTS QGASDPLPDP LGLMDLSTTP LQSAPPLESP QRLLSSEPLD 730 740 750 760 770 780 LISVPFGNSS PSDIDVPKPG SPEPQVSGLA ANRSLTEGLV LDTMNDSLSK ILLDISFPGL 790 800 DEDPLGPDNI NWSQFIPELQ « Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Hepatocyte nuclear factor 3/fork head homolog 11 is expressed in proliferating epithelial and mesenchymal cells of embryonic and adult tissues." Ye H., Kelly T.F., Samadani U., Lim L., Rubio S., Overdier D.G., Roebuck K.A., Costa R.H. Mol. Cell. Biol. 17:1626-1641(1997) [PubMed: 9032290] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 4). Tissue: Colon carcinoma.
[2]	"Molecular analysis of a novel winged helix protein, WIN. Expression pattern, DNA binding property, and alternative splicing within the DNA binding domain." Yao K.-M., Sha M., Lu Z., Wong G.G. J. Biol. Chem. 272:19827-19836(1997) [PubMed: 9242644] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Pancreatic carcinoma and Testis.
[3]	"NIEHS-SNPs, environmental genome project, NIEHS ES15478, Department of Genome Sciences, Seattle, WA (URL: http://egp.gs.washington.edu)." Livingston R.J., Rieder M.J., Shaffer T., Bertucci C., Baier C.N., Rajkumar N., Willa H.T., Daniels M., Downing T.K., Stanaway I.B., Nguyen C.P., Gildersleeve H., Cassidy C.M., Johnson E.J., Swanson J.E., McFarland I., Yool B., Park C., Nickerson D.A. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS GLU-402; LEU-450; PRO-643; ARG-669 AND LEU-673.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT PRO-643. Tissue: Lung, Ovary and Skin.
[5]	"Cloning of cDNAs for M-phase phosphoproteins recognized by the MPM2 monoclonal antibody and determination of the phosphorylated epitope." Westendorf J.M., Rao P.N., Gerace L. Proc. Natl. Acad. Sci. U.S.A. 91:714-718(1994) [PubMed: 8290587] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 543-763. Tissue: Lymphoblast.
[6]	"Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-704, MASS SPECTROMETRY. Tissue: Epithelium.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-331; THR-620 AND THR-627, MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	U74612 mRNA. Translation: AAC51128.1. U74613 mRNA. Translation: AAC51129.1. U83113 mRNA. Translation: AAC63595.1. DQ022289 Genomic DNA. Translation: AAY26401.1. BC006192 mRNA. Translation: AAH06192.1. BC006529 mRNA. Translation: AAH06529.1. BC012863 mRNA. Translation: AAH12863.1. L16783 mRNA. Translation: AAC37541.1.
PIR	B36881.
RefSeq	NP_068772.2. NP_973731.1. NP_973732.1.
UniGene	Hs.239
3D structure databases
HSSP	HSSP built from PDB template 1E17 based on UniProtKB P98177.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q08050.
PTM databases
PhosphoSite	Q08050.
Polymorphism databases
NIEHS-SNPs	Search...
Genome annotation databases
Ensembl	ENSG00000111206. Homo sapiens. [Contig view]
GeneID	2305.
KEGG	hsa:2305.
Organism-specific databases
H-InvDB	HIX0010334.
HGNC	HGNC:3818. FOXM1.
HPA	CAB017832.
MIM	602341. gene.
PharmGKB	PA28236.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOVERGEN	Q08050.
Gene expression databases
ArrayExpress	Q08050.
CleanEx	HS_FOXM1. HS_MPP2.
GermOnline	ENSG00000111206. Homo sapiens.
Family and domain databases
InterPro	IPR001766. TF_Fork_head. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
PANTHER	PTHR11829. Fork_box_protein. 1 hit.
Pfam	PF00250. Fork_head. 1 hit. [Graphical view]
PRINTS	PR00053. FORKHEAD.
ProDom	PD000425. TF_Fork_head. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00339. FH. 1 hit. [Graphical view]
PROSITE	PS00657. FORK_HEAD_1. 1 hit. PS00658. FORK_HEAD_2. 1 hit. PS50039. FORK_HEAD_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	9359.
SOURCE	Search...

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Entry information

Entry name

FOXM1_HUMAN

Accession

Primary (citable) accession number: Q08050
Secondary accession number(s): O43258

Q9BRL2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	December 1, 2000
Last modified:	November 25, 2008
This is version 91 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Keywords

Gene Ontology (GO)

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents