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Protein

Hepatocyte growth factor

Gene

Hgf

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Growth factor, Serine protease homolog

Enzyme and pathway databases

ReactomeiR-MMU-114608. Platelet degranulation.
R-MMU-1257604. PIP3 activates AKT signaling.
R-MMU-5673001. RAF/MAP kinase cascade.
R-MMU-6806942. MET Receptor Activation.
R-MMU-6807004. Negative regulation of MET activity.
R-MMU-6811558. PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling.
R-MMU-8851805. MET activates RAS signaling.
R-MMU-8851907. MET activates PI3K/AKT signaling.
R-MMU-8865999. MET activates PTPN11.
R-MMU-8874081. MET activates PTK2 signaling.
R-MMU-8875513. MET interacts with TNS proteins.
R-MMU-8875555. MET activates RAP1 and RAC1.
R-MMU-8875656. MET receptor recycling.
R-MMU-8875791. MET activates STAT3.

Protein family/group databases

MEROPSiS01.982.

Names & Taxonomyi

Protein namesi
Recommended name:
Hepatocyte growth factor
Alternative name(s):
Hepatopoietin-A
Scatter factor
Short name:
SF
Cleaved into the following 2 chains:
Gene namesi
Name:Hgf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:96079. Hgf.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 32By similarityAdd BLAST32
ChainiPRO_000002809333 – 495Hepatocyte growth factor alpha chainAdd BLAST463
ChainiPRO_0000028094496 – 728Hepatocyte growth factor beta chainAdd BLAST233

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei33Pyrrolidone carboxylic acidBy similarity1
Disulfide bondi71 ↔ 97
Disulfide bondi75 ↔ 85
Disulfide bondi129 ↔ 207
Disulfide bondi150 ↔ 190
Disulfide bondi178 ↔ 202
Disulfide bondi212 ↔ 289By similarity
Disulfide bondi233 ↔ 272By similarity
Disulfide bondi261 ↔ 284By similarity
Glycosylationi295N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi306 ↔ 384By similarity
Disulfide bondi327 ↔ 366By similarity
Disulfide bondi355 ↔ 378By similarity
Disulfide bondi392 ↔ 470By similarity
Glycosylationi403N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi413 ↔ 453By similarity
Disulfide bondi441 ↔ 465By similarity
Disulfide bondi488 ↔ 607Interchain (between alpha and beta chains)PROSITE-ProRule annotation
Disulfide bondi520 ↔ 536By similarity
Glycosylationi569N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi615 ↔ 682By similarity
Disulfide bondi645 ↔ 661By similarity
Glycosylationi656N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi672 ↔ 700By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Pyrrolidone carboxylic acid

Proteomic databases

PaxDbiQ08048.
PRIDEiQ08048.

PTM databases

PhosphoSitePlusiQ08048.

Expressioni

Gene expression databases

BgeeiENSMUSG00000028864.
CleanExiMM_HGF.
GenevisibleiQ08048. MM.

Interactioni

Subunit structurei

Dimer of an alpha chain and a beta chain linked by a disulfide bond. Interacts with SRPX2; the interaction increases HGF mitogenic activity.

Protein-protein interaction databases

DIPiDIP-46456N.
STRINGi10090.ENSMUSP00000030683.

Structurei

Secondary structure

1728
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi40 – 42Combined sources3
Beta strandi43 – 52Combined sources10
Beta strandi55 – 57Combined sources3
Beta strandi61 – 64Combined sources4
Helixi68 – 77Combined sources10
Turni78 – 80Combined sources3
Beta strandi87 – 91Combined sources5
Turni92 – 95Combined sources4
Beta strandi96 – 101Combined sources6
Beta strandi106 – 108Combined sources3
Beta strandi110 – 122Combined sources13
Helixi123 – 125Combined sources3
Beta strandi129 – 133Combined sources5
Beta strandi149 – 151Combined sources3
Beta strandi157 – 159Combined sources3
Turni165 – 167Combined sources3
Beta strandi188 – 194Combined sources7
Beta strandi198 – 201Combined sources4
Turni207 – 209Combined sources3
Beta strandi240 – 242Combined sources3
Helixi248 – 250Combined sources3
Turni252 – 255Combined sources4
Beta strandi271 – 275Combined sources5
Beta strandi280 – 283Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2QJ4X-ray2.50A/B29-210[»]
3HMRX-ray2.00A31-127[»]
4IUAX-ray3.05A/B/C/D/E/F/G/H31-290[»]
ProteinModelPortaliQ08048.
SMRiQ08048.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ08048.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini38 – 124PANPROSITE-ProRule annotationAdd BLAST87
Domaini129 – 207Kringle 1PROSITE-ProRule annotationAdd BLAST79
Domaini212 – 289Kringle 2PROSITE-ProRule annotationAdd BLAST78
Domaini306 – 384Kringle 3PROSITE-ProRule annotationAdd BLAST79
Domaini392 – 470Kringle 4PROSITE-ProRule annotationAdd BLAST79
Domaini496 – 724Peptidase S1PROSITE-ProRule annotationAdd BLAST229

Sequence similaritiesi

Belongs to the peptidase S1 family. Plasminogen subfamily.PROSITE-ProRule annotation
Contains 4 kringle domains.PROSITE-ProRule annotation
Contains 1 PAN domain.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Kringle, Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IDXR. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000119133.
HOGENOMiHOG000112892.
HOVERGENiHBG004381.
InParanoidiQ08048.
KOiK05460.
OMAiGSESPWC.
OrthoDBiEOG091G0AH5.
PhylomeDBiQ08048.
TreeFamiTF329901.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR027284. Hepatocyte_GF.
IPR024174. HGF-like.
IPR000001. Kringle.
IPR013806. Kringle-like.
IPR018056. Kringle_CS.
IPR003609. Pan_app.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
[Graphical view]
PfamiPF00051. Kringle. 4 hits.
PF00024. PAN_1. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFiPIRSF500183. Hepatocyte_GF. 1 hit.
PIRSF001152. HGF_MST1. 1 hit.
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00130. KR. 4 hits.
SM00473. PAN_AP. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF57440. SSF57440. 4 hits.
PROSITEiPS00021. KRINGLE_1. 4 hits.
PS50070. KRINGLE_2. 4 hits.
PS50948. PAN. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: Q08048-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMWGTKLLPV LLLQHVLLHL LLLHVAIPYA EGQKKRRNTL HEFKKSAKTT
60 70 80 90 100
LTKEDPLLKI KTKKVNSADE CANRCIRNRG FTFTCKAFVF DKSRKRCYWY
110 120 130 140 150
PFNSMSSGVK KGFGHEFDLY ENKDYIRNCI IGKGGSYKGT VSITKSGIKC
160 170 180 190 200
QPWNSMIPHE HSFLPSSYRG KDLQENYCRN PRGEEGGPWC FTSNPEVRYE
210 220 230 240 250
VCDIPQCSEV ECMTCNGESY RGPMDHTESG KTCQRWDQQT PHRHKFLPER
260 270 280 290 300
YPDKGFDDNY CRNPDGKPRP WCYTLDPDTP WEYCAIKTCA HSAVNETDVP
310 320 330 340 350
METTECIQGQ GEGYRGTSNT IWNGIPCQRW DSQYPHKHDI TPENFKCKDL
360 370 380 390 400
RENYCRNPDG AESPWCFTTD PNIRVGYCSQ IPKCDVSSGQ DCYRGNGKNY
410 420 430 440 450
MGNLSKTRSG LTCSMWDKNM EDLHRHIFWE PDASKLNKNY CRNPDDDAHG
460 470 480 490 500
PWCYTGNPLI PWDYCPISRC EGDTTPTIVN LDHPVISCAK TKQLRVVNGI
510 520 530 540 550
PTQTTVGWMV SLKYRNKHIC GGSLIKESWV LTARQCFPAR NKDLKDYEAW
560 570 580 590 600
LGIHDVHERG EEKRKQILNI SQLVYGPEGS DLVLLKLARP AILDNFVSTI
610 620 630 640 650
DLPSYGCTIP EKTTCSIYGW GYTGLINADG LLRVAHLYIM GNEKCSQHHQ
660 670 680 690 700
GKVTLNESEL CAGAEKIGSG PCEGDYGGPL ICEQHKMRMV LGVIVPGRGC
710 720
AIPNRPGIFV RVAYYAKWIH KVILTYKL
Length:728
Mass (Da):82,945
Last modified:November 1, 1995 - v1
Checksum:iA0381FC497534328
GO
Isoform Short (identifier: Q08048-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     163-167: Missing.

Show »
Length:723
Mass (Da):82,413
Checksum:i7BC19A16C399EDD2
GO
Isoform NK1 (identifier: Q08048-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     210-211: VE → GK
     212-728: Missing.

Show »
Length:211
Mass (Da):24,308
Checksum:i758BD0687A835F48
GO

Sequence cautioni

The sequence CAA51054 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti189W → K AA sequence (PubMed:2144630).Curated1
Sequence conflicti344N → K in AAB31855 (PubMed:8081873).Curated1
Sequence conflicti378C → E AA sequence (PubMed:2144630).Curated1
Sequence conflicti380Q → E AA sequence (PubMed:2144630).Curated1
Sequence conflicti479V → L in AAB31855 (PubMed:8081873).Curated1
Sequence conflicti513K → L AA sequence (PubMed:2142751).Curated1
Sequence conflicti518H → T AA sequence (PubMed:1831975).Curated1
Sequence conflicti564R → H in CAA51054 (PubMed:8241272).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_005408163 – 167Missing in isoform Short. 1 Publication5
Alternative sequenceiVSP_044345210 – 211VE → GK in isoform NK1. 1 Publication2
Alternative sequenceiVSP_044346212 – 728Missing in isoform NK1. 1 PublicationAdd BLAST517

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10212 mRNA. Translation: BAA01064.1.
D10213 mRNA. Translation: BAA01065.1.
S71816 mRNA. Translation: AAB31855.1.
X72307 mRNA. Translation: CAA51054.1. Different initiation.
AF042856 mRNA. Translation: AAC40051.1.
X84046 mRNA. Translation: CAA58865.1.
CH466586 Genomic DNA. Translation: EDL03238.1.
BC119228 mRNA. Translation: AAI19229.1.
X81630 Genomic DNA. Translation: CAA57286.1.
CCDSiCCDS19097.1. [Q08048-1]
CCDS80213.1. [Q08048-2]
PIRiJC2117. A60185.
RefSeqiNP_001276387.1. NM_001289458.1. [Q08048-1]
NP_001276388.1. NM_001289459.1. [Q08048-1]
NP_001276389.1. NM_001289460.2. [Q08048-3]
NP_001276390.1. NM_001289461.1. [Q08048-2]
NP_034557.3. NM_010427.5. [Q08048-1]
UniGeneiMm.267078.

Genome annotation databases

EnsembliENSMUST00000030683; ENSMUSP00000030683; ENSMUSG00000028864. [Q08048-1]
ENSMUST00000196645; ENSMUSP00000142517; ENSMUSG00000028864. [Q08048-2]
ENSMUST00000199581; ENSMUSP00000143424; ENSMUSG00000028864. [Q08048-1]
GeneIDi15234.
KEGGimmu:15234.
UCSCiuc008wne.2. mouse. [Q08048-3]
uc008wnf.2. mouse. [Q08048-1]
uc008wnj.1. mouse. [Q08048-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10212 mRNA. Translation: BAA01064.1.
D10213 mRNA. Translation: BAA01065.1.
S71816 mRNA. Translation: AAB31855.1.
X72307 mRNA. Translation: CAA51054.1. Different initiation.
AF042856 mRNA. Translation: AAC40051.1.
X84046 mRNA. Translation: CAA58865.1.
CH466586 Genomic DNA. Translation: EDL03238.1.
BC119228 mRNA. Translation: AAI19229.1.
X81630 Genomic DNA. Translation: CAA57286.1.
CCDSiCCDS19097.1. [Q08048-1]
CCDS80213.1. [Q08048-2]
PIRiJC2117. A60185.
RefSeqiNP_001276387.1. NM_001289458.1. [Q08048-1]
NP_001276388.1. NM_001289459.1. [Q08048-1]
NP_001276389.1. NM_001289460.2. [Q08048-3]
NP_001276390.1. NM_001289461.1. [Q08048-2]
NP_034557.3. NM_010427.5. [Q08048-1]
UniGeneiMm.267078.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2QJ4X-ray2.50A/B29-210[»]
3HMRX-ray2.00A31-127[»]
4IUAX-ray3.05A/B/C/D/E/F/G/H31-290[»]
ProteinModelPortaliQ08048.
SMRiQ08048.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-46456N.
STRINGi10090.ENSMUSP00000030683.

Protein family/group databases

MEROPSiS01.982.

PTM databases

PhosphoSitePlusiQ08048.

Proteomic databases

PaxDbiQ08048.
PRIDEiQ08048.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030683; ENSMUSP00000030683; ENSMUSG00000028864. [Q08048-1]
ENSMUST00000196645; ENSMUSP00000142517; ENSMUSG00000028864. [Q08048-2]
ENSMUST00000199581; ENSMUSP00000143424; ENSMUSG00000028864. [Q08048-1]
GeneIDi15234.
KEGGimmu:15234.
UCSCiuc008wne.2. mouse. [Q08048-3]
uc008wnf.2. mouse. [Q08048-1]
uc008wnj.1. mouse. [Q08048-2]

Organism-specific databases

CTDi3082.
MGIiMGI:96079. Hgf.

Phylogenomic databases

eggNOGiENOG410IDXR. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000119133.
HOGENOMiHOG000112892.
HOVERGENiHBG004381.
InParanoidiQ08048.
KOiK05460.
OMAiGSESPWC.
OrthoDBiEOG091G0AH5.
PhylomeDBiQ08048.
TreeFamiTF329901.

Enzyme and pathway databases

ReactomeiR-MMU-114608. Platelet degranulation.
R-MMU-1257604. PIP3 activates AKT signaling.
R-MMU-5673001. RAF/MAP kinase cascade.
R-MMU-6806942. MET Receptor Activation.
R-MMU-6807004. Negative regulation of MET activity.
R-MMU-6811558. PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling.
R-MMU-8851805. MET activates RAS signaling.
R-MMU-8851907. MET activates PI3K/AKT signaling.
R-MMU-8865999. MET activates PTPN11.
R-MMU-8874081. MET activates PTK2 signaling.
R-MMU-8875513. MET interacts with TNS proteins.
R-MMU-8875555. MET activates RAP1 and RAC1.
R-MMU-8875656. MET receptor recycling.
R-MMU-8875791. MET activates STAT3.

Miscellaneous databases

EvolutionaryTraceiQ08048.
PROiQ08048.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028864.
CleanExiMM_HGF.
GenevisibleiQ08048. MM.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR027284. Hepatocyte_GF.
IPR024174. HGF-like.
IPR000001. Kringle.
IPR013806. Kringle-like.
IPR018056. Kringle_CS.
IPR003609. Pan_app.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
[Graphical view]
PfamiPF00051. Kringle. 4 hits.
PF00024. PAN_1. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFiPIRSF500183. Hepatocyte_GF. 1 hit.
PIRSF001152. HGF_MST1. 1 hit.
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00130. KR. 4 hits.
SM00473. PAN_AP. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF57440. SSF57440. 4 hits.
PROSITEiPS00021. KRINGLE_1. 4 hits.
PS50070. KRINGLE_2. 4 hits.
PS50948. PAN. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHGF_MOUSE
AccessioniPrimary (citable) accession number: Q08048
Secondary accession number(s): O55027
, Q53WS5, Q61662, Q64007, Q6LBE6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 163 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Has lost two of the three essential catalytic residues and so probably has no enzymatic activity.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.