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Q08047 (GLGB_MAIZE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan-branching enzyme 2, chloroplastic/amyloplastic

EC=2.4.1.18
Alternative name(s):
Q-enzyme
Starch-branching enzyme IIB
Gene names
Name:SBE1
OrganismZea mays (Maize)
Taxonomic identifier4577 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

Protein attributes

Sequence length799 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in starch by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

Pathway

Glycan biosynthesis; starch biosynthesis.

Subunit structure

Monomer By similarity.

Subcellular location

Plastidchloroplast. Plastidamyloplast.

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 5757Chloroplast; amyloplast Ref.1
Chain58 – 7997421,4-alpha-glucan-branching enzyme 2, chloroplastic/amyloplastic
PRO_0000011147

Sites

Active site4471Nucleophile By similarity
Active site5021Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q08047 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 0B440E0377B8087A

FASTA79990,518
        10         20         30         40         50         60 
MAFRVSGAVL GGAVRAPRLT GGGEGSLVFR HTGLFLTRGA RVGCSGTHGA MRAAAAARKA 

        70         80         90        100        110        120 
VMVPEGENDG LASRADSAQF QSDELEVPDI SEETTCGAGV ADAQALNRVR VVPPPSDGQK 

       130        140        150        160        170        180 
IFQIDPMLQG YKYHLEYRYS LYRRIRSDID EHEGGLEAFS RSYEKFGFNA SAEGITYREW 

       190        200        210        220        230        240 
APGAFSAALV GDVNNWDPNA DRMSKNEFGV WEIFLPNNAD GTSPIPHGSR VKVRMDTPSG 

       250        260        270        280        290        300 
IKDSIPAWIK YSVQAPGEIP YDGIYYDPPE EVKYVFRHAQ PKRPKSLRIY ETHVGMSSPE 

       310        320        330        340        350        360 
PKINTYVNFR DEVLPRIKKL GYNAVQIMAI QEHSYYGSFG YHVTNFFAPS SRFGTPEDLK 

       370        380        390        400        410        420 
SLIDRAHELG LLVLMDVVHS HASSNTLDGL NGFDGTDTHY FHSGPRGHHW MWDSRLFNYG 

       430        440        450        460        470        480 
NWEVLRFLLS NARWWLEEYK FDGFRFDGVT SMMYTHHGLQ VTFTGNFNEY FGFATDVDAV 

       490        500        510        520        530        540 
VYLMLVNDLI HGLYPEAVTI GEDVSGMPTF ALPVHDGGVG FDYRMHMAVA DKWIDLLKQS 

       550        560        570        580        590        600 
DETWKMGDIV HTLTNRRWLE KCVTYAESHD QALVGDKTIA FWLMDKDMYD FMALDRPSTP 

       610        620        630        640        650        660 
TIDRGIALHK MIRLITMGLG GEGYLNFMGN EFGHPEWIDF PRGPQRLPSG KFIPGNNNSY 

       670        680        690        700        710        720 
DKCRRRFDLG DADYLRYHGM QEFDQAMQHL EQKYEFMTSD HQYISRKHEE DKVIVFEKGD 

       730        740        750        760        770        780 
LVFVFNFHCN NSYFDYRIGC RKPGVYKVVL DSDAGLFGGF SRIHHAAEHF TADCSHDNRP 

       790 
YSFSVYTPSR TCVVYAPVE 

« Hide

References

[1]"Starch branching enzyme II from maize endosperm."
Fisher D.K., Boyer C.D., Hannah L.C.
Plant Physiol. 102:1045-1046(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 58-65.
Strain: cv. W64A X 182E.
Tissue: Endosperm.
[2]"Expression of branching enzyme II of maize endosperm in Escherichia coli."
Guan H.P., Baba T., Preiss J.
Cell. Mol. Biol. 40:981-988(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 248-271 AND 305-315.
Strain: cv. B73.
Tissue: Endosperm.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L08065 mRNA. Translation: AAA18571.1.
PIRT02981.
RefSeqNP_001105316.1. NM_001111846.1.
UniGeneZm.143.

3D structure databases

ProteinModelPortalQ08047.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Proteomic databases

PRIDEQ08047.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID542238.
KEGGzma:542238.

Organism-specific databases

GrameneQ08047.
MaizeGDB63943.

Phylogenomic databases

HOGENOMHOG000175159.
KOK00700.

Enzyme and pathway databases

UniPathwayUPA00152.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_MAIZE
AccessionPrimary (citable) accession number: Q08047
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 9, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries