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Q08047

- GLGB_MAIZE

UniProt

Q08047 - GLGB_MAIZE

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Protein

1,4-alpha-glucan-branching enzyme 2, chloroplastic/amyloplastic

Gene

SBE1

Organism
Zea mays (Maize)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in starch by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei447 – 4471NucleophileBy similarity
Active sitei502 – 5021Proton donorBy similarity

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-EC
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. glycogen biosynthetic process Source: InterPro
  2. starch biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Starch biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00152.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan-branching enzyme 2, chloroplastic/amyloplastic (EC:2.4.1.18)
Alternative name(s):
Q-enzyme
Starch-branching enzyme IIB
Gene namesi
Name:SBE1
OrganismiZea mays (Maize)
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

Organism-specific databases

GrameneiQ08047.
MaizeGDBi63943.

Subcellular locationi

GO - Cellular componenti

  1. amyloplast Source: UniProtKB-KW
  2. chloroplast Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Amyloplast, Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5757Chloroplast; amyloplast1 PublicationAdd
BLAST
Chaini58 – 7997421,4-alpha-glucan-branching enzyme 2, chloroplastic/amyloplasticPRO_0000011147Add
BLAST

Proteomic databases

PRIDEiQ08047.

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ08047.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000175159.
KOiK00700.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q08047-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAFRVSGAVL GGAVRAPRLT GGGEGSLVFR HTGLFLTRGA RVGCSGTHGA
60 70 80 90 100
MRAAAAARKA VMVPEGENDG LASRADSAQF QSDELEVPDI SEETTCGAGV
110 120 130 140 150
ADAQALNRVR VVPPPSDGQK IFQIDPMLQG YKYHLEYRYS LYRRIRSDID
160 170 180 190 200
EHEGGLEAFS RSYEKFGFNA SAEGITYREW APGAFSAALV GDVNNWDPNA
210 220 230 240 250
DRMSKNEFGV WEIFLPNNAD GTSPIPHGSR VKVRMDTPSG IKDSIPAWIK
260 270 280 290 300
YSVQAPGEIP YDGIYYDPPE EVKYVFRHAQ PKRPKSLRIY ETHVGMSSPE
310 320 330 340 350
PKINTYVNFR DEVLPRIKKL GYNAVQIMAI QEHSYYGSFG YHVTNFFAPS
360 370 380 390 400
SRFGTPEDLK SLIDRAHELG LLVLMDVVHS HASSNTLDGL NGFDGTDTHY
410 420 430 440 450
FHSGPRGHHW MWDSRLFNYG NWEVLRFLLS NARWWLEEYK FDGFRFDGVT
460 470 480 490 500
SMMYTHHGLQ VTFTGNFNEY FGFATDVDAV VYLMLVNDLI HGLYPEAVTI
510 520 530 540 550
GEDVSGMPTF ALPVHDGGVG FDYRMHMAVA DKWIDLLKQS DETWKMGDIV
560 570 580 590 600
HTLTNRRWLE KCVTYAESHD QALVGDKTIA FWLMDKDMYD FMALDRPSTP
610 620 630 640 650
TIDRGIALHK MIRLITMGLG GEGYLNFMGN EFGHPEWIDF PRGPQRLPSG
660 670 680 690 700
KFIPGNNNSY DKCRRRFDLG DADYLRYHGM QEFDQAMQHL EQKYEFMTSD
710 720 730 740 750
HQYISRKHEE DKVIVFEKGD LVFVFNFHCN NSYFDYRIGC RKPGVYKVVL
760 770 780 790
DSDAGLFGGF SRIHHAAEHF TADCSHDNRP YSFSVYTPSR TCVVYAPVE
Length:799
Mass (Da):90,518
Last modified:November 1, 1995 - v1
Checksum:i0B440E0377B8087A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L08065 mRNA. Translation: AAA18571.1.
PIRiT02981.
RefSeqiNP_001105316.1. NM_001111846.1.
UniGeneiZm.143.

Genome annotation databases

GeneIDi542238.
KEGGizma:542238.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L08065 mRNA. Translation: AAA18571.1 .
PIRi T02981.
RefSeqi NP_001105316.1. NM_001111846.1.
UniGenei Zm.143.

3D structure databases

ProteinModelPortali Q08047.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi CBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Proteomic databases

PRIDEi Q08047.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 542238.
KEGGi zma:542238.

Organism-specific databases

Gramenei Q08047.
MaizeGDBi 63943.

Phylogenomic databases

HOGENOMi HOG000175159.
KOi K00700.

Enzyme and pathway databases

UniPathwayi UPA00152 .

Family and domain databases

Gene3Di 2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view ]
PIRSFi PIRSF000463. GlgB. 1 hit.
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Starch branching enzyme II from maize endosperm."
    Fisher D.K., Boyer C.D., Hannah L.C.
    Plant Physiol. 102:1045-1046(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 58-65.
    Strain: cv. W64A X 182E.
    Tissue: Endosperm.
  2. "Expression of branching enzyme II of maize endosperm in Escherichia coli."
    Guan H.P., Baba T., Preiss J.
    Cell. Mol. Biol. 40:981-988(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 248-271 AND 305-315.
    Strain: cv. B73.
    Tissue: Endosperm.

Entry informationi

Entry nameiGLGB_MAIZE
AccessioniPrimary (citable) accession number: Q08047
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 29, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3