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Protein

1,4-alpha-glucan-branching enzyme 2, chloroplastic/amyloplastic

Gene

SBE1

Organism
Zea mays (Maize)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in starch by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

Pathwayi: starch biosynthesis

This protein is involved in the pathway starch biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway starch biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei447NucleophileBy similarity1
Active sitei502Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processStarch biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00152

Protein family/group databases

CAZyiCBM48 Carbohydrate-Binding Module Family 48
GH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan-branching enzyme 2, chloroplastic/amyloplastic (EC:2.4.1.18)
Alternative name(s):
Q-enzyme
Starch-branching enzyme IIB
Gene namesi
Name:SBE1
OrganismiZea mays (Maize)
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogonodaeAndropogoneaeTripsacinaeZea
Proteomesi

Organism-specific databases

MaizeGDBi63943

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Amyloplast, Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 57Chloroplast; amyloplast1 PublicationAdd BLAST57
ChainiPRO_000001114758 – 7991,4-alpha-glucan-branching enzyme 2, chloroplastic/amyloplasticAdd BLAST742

Proteomic databases

PaxDbiQ08047
PRIDEiQ08047

Expressioni

Gene expression databases

ExpressionAtlasiQ08047 differential

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi4577.GRMZM2G032628_P01

Structurei

3D structure databases

ProteinModelPortaliQ08047
SMRiQ08047
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0470 Eukaryota
COG0296 LUCA
HOGENOMiHOG000175159
KOiK00700

Family and domain databases

Gene3Di2.60.40.10, 1 hit
2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR006048 A-amylase/branching_C
IPR037439 Branching_enzy
IPR006047 Glyco_hydro_13_cat_dom
IPR004193 Glyco_hydro_13_N
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
PANTHERiPTHR43651 PTHR43651, 1 hit
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02806 Alpha-amylase_C, 1 hit
PF02922 CBM_48, 1 hit
PIRSFiPIRSF000463 GlgB, 1 hit
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
SSF81296 SSF81296, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q08047-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFRVSGAVL GGAVRAPRLT GGGEGSLVFR HTGLFLTRGA RVGCSGTHGA
60 70 80 90 100
MRAAAAARKA VMVPEGENDG LASRADSAQF QSDELEVPDI SEETTCGAGV
110 120 130 140 150
ADAQALNRVR VVPPPSDGQK IFQIDPMLQG YKYHLEYRYS LYRRIRSDID
160 170 180 190 200
EHEGGLEAFS RSYEKFGFNA SAEGITYREW APGAFSAALV GDVNNWDPNA
210 220 230 240 250
DRMSKNEFGV WEIFLPNNAD GTSPIPHGSR VKVRMDTPSG IKDSIPAWIK
260 270 280 290 300
YSVQAPGEIP YDGIYYDPPE EVKYVFRHAQ PKRPKSLRIY ETHVGMSSPE
310 320 330 340 350
PKINTYVNFR DEVLPRIKKL GYNAVQIMAI QEHSYYGSFG YHVTNFFAPS
360 370 380 390 400
SRFGTPEDLK SLIDRAHELG LLVLMDVVHS HASSNTLDGL NGFDGTDTHY
410 420 430 440 450
FHSGPRGHHW MWDSRLFNYG NWEVLRFLLS NARWWLEEYK FDGFRFDGVT
460 470 480 490 500
SMMYTHHGLQ VTFTGNFNEY FGFATDVDAV VYLMLVNDLI HGLYPEAVTI
510 520 530 540 550
GEDVSGMPTF ALPVHDGGVG FDYRMHMAVA DKWIDLLKQS DETWKMGDIV
560 570 580 590 600
HTLTNRRWLE KCVTYAESHD QALVGDKTIA FWLMDKDMYD FMALDRPSTP
610 620 630 640 650
TIDRGIALHK MIRLITMGLG GEGYLNFMGN EFGHPEWIDF PRGPQRLPSG
660 670 680 690 700
KFIPGNNNSY DKCRRRFDLG DADYLRYHGM QEFDQAMQHL EQKYEFMTSD
710 720 730 740 750
HQYISRKHEE DKVIVFEKGD LVFVFNFHCN NSYFDYRIGC RKPGVYKVVL
760 770 780 790
DSDAGLFGGF SRIHHAAEHF TADCSHDNRP YSFSVYTPSR TCVVYAPVE
Length:799
Mass (Da):90,518
Last modified:November 1, 1995 - v1
Checksum:i0B440E0377B8087A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08065 mRNA Translation: AAA18571.1
PIRiT02981
RefSeqiNP_001105316.1, NM_001111846.2
UniGeneiZm.143

Genome annotation databases

GeneIDi542238
KEGGizma:542238

Similar proteinsi

Entry informationi

Entry nameiGLGB_MAIZE
AccessioniPrimary (citable) accession number: Q08047
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 23, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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