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Q08047

- GLGB_MAIZE

UniProt

Q08047 - GLGB_MAIZE

Protein

1,4-alpha-glucan-branching enzyme 2, chloroplastic/amyloplastic

Gene

SBE1

Organism
Zea mays (Maize)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of the alpha-1,6-glucosidic linkages in starch by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.

    Catalytic activityi

    Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei447 – 4471NucleophileBy similarity
    Active sitei502 – 5021Proton donorBy similarity

    GO - Molecular functioni

    1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-EC
    2. cation binding Source: InterPro
    3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

    GO - Biological processi

    1. cellular response to fructose stimulus Source: EnsemblPlants/Gramene
    2. cellular response to glucose stimulus Source: EnsemblPlants/Gramene
    3. cellular response to light stimulus Source: EnsemblPlants/Gramene
    4. cellular response to sucrose stimulus Source: EnsemblPlants/Gramene
    5. glycogen biosynthetic process Source: InterPro
    6. starch biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Starch biosynthesis

    Enzyme and pathway databases

    UniPathwayiUPA00152.

    Protein family/group databases

    CAZyiCBM48. Carbohydrate-Binding Module Family 48.
    GH13. Glycoside Hydrolase Family 13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1,4-alpha-glucan-branching enzyme 2, chloroplastic/amyloplastic (EC:2.4.1.18)
    Alternative name(s):
    Q-enzyme
    Starch-branching enzyme IIB
    Gene namesi
    Name:SBE1
    OrganismiZea mays (Maize)
    Taxonomic identifieri4577 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

    Organism-specific databases

    GrameneiQ08047.
    MaizeGDBi63943.

    Subcellular locationi

    GO - Cellular componenti

    1. amyloplast Source: UniProtKB-SubCell
    2. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Amyloplast, Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 5757Chloroplast; amyloplast1 PublicationAdd
    BLAST
    Chaini58 – 7997421,4-alpha-glucan-branching enzyme 2, chloroplastic/amyloplasticPRO_0000011147Add
    BLAST

    Proteomic databases

    PRIDEiQ08047.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ08047.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    HOGENOMiHOG000175159.
    KOiK00700.

    Family and domain databases

    Gene3Di2.60.40.10. 1 hit.
    2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR006048. A-amylase_b_C.
    IPR006407. GlgB.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004193. Glyco_hydro_13_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 1 hit.
    PfamiPF00128. Alpha-amylase. 1 hit.
    PF02806. Alpha-amylase_C. 1 hit.
    PF02922. CBM_48. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000463. GlgB. 1 hit.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF81296. SSF81296. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q08047-1 [UniParc]FASTAAdd to Basket

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    MAFRVSGAVL GGAVRAPRLT GGGEGSLVFR HTGLFLTRGA RVGCSGTHGA    50
    MRAAAAARKA VMVPEGENDG LASRADSAQF QSDELEVPDI SEETTCGAGV 100
    ADAQALNRVR VVPPPSDGQK IFQIDPMLQG YKYHLEYRYS LYRRIRSDID 150
    EHEGGLEAFS RSYEKFGFNA SAEGITYREW APGAFSAALV GDVNNWDPNA 200
    DRMSKNEFGV WEIFLPNNAD GTSPIPHGSR VKVRMDTPSG IKDSIPAWIK 250
    YSVQAPGEIP YDGIYYDPPE EVKYVFRHAQ PKRPKSLRIY ETHVGMSSPE 300
    PKINTYVNFR DEVLPRIKKL GYNAVQIMAI QEHSYYGSFG YHVTNFFAPS 350
    SRFGTPEDLK SLIDRAHELG LLVLMDVVHS HASSNTLDGL NGFDGTDTHY 400
    FHSGPRGHHW MWDSRLFNYG NWEVLRFLLS NARWWLEEYK FDGFRFDGVT 450
    SMMYTHHGLQ VTFTGNFNEY FGFATDVDAV VYLMLVNDLI HGLYPEAVTI 500
    GEDVSGMPTF ALPVHDGGVG FDYRMHMAVA DKWIDLLKQS DETWKMGDIV 550
    HTLTNRRWLE KCVTYAESHD QALVGDKTIA FWLMDKDMYD FMALDRPSTP 600
    TIDRGIALHK MIRLITMGLG GEGYLNFMGN EFGHPEWIDF PRGPQRLPSG 650
    KFIPGNNNSY DKCRRRFDLG DADYLRYHGM QEFDQAMQHL EQKYEFMTSD 700
    HQYISRKHEE DKVIVFEKGD LVFVFNFHCN NSYFDYRIGC RKPGVYKVVL 750
    DSDAGLFGGF SRIHHAAEHF TADCSHDNRP YSFSVYTPSR TCVVYAPVE 799
    Length:799
    Mass (Da):90,518
    Last modified:November 1, 1995 - v1
    Checksum:i0B440E0377B8087A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L08065 mRNA. Translation: AAA18571.1.
    PIRiT02981.
    RefSeqiNP_001105316.1. NM_001111846.1.
    UniGeneiZm.143.

    Genome annotation databases

    GeneIDi542238.
    KEGGizma:542238.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L08065 mRNA. Translation: AAA18571.1 .
    PIRi T02981.
    RefSeqi NP_001105316.1. NM_001111846.1.
    UniGenei Zm.143.

    3D structure databases

    ProteinModelPortali Q08047.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi CBM48. Carbohydrate-Binding Module Family 48.
    GH13. Glycoside Hydrolase Family 13.

    Proteomic databases

    PRIDEi Q08047.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 542238.
    KEGGi zma:542238.

    Organism-specific databases

    Gramenei Q08047.
    MaizeGDBi 63943.

    Phylogenomic databases

    HOGENOMi HOG000175159.
    KOi K00700.

    Enzyme and pathway databases

    UniPathwayi UPA00152 .

    Family and domain databases

    Gene3Di 2.60.40.10. 1 hit.
    2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR006048. A-amylase_b_C.
    IPR006407. GlgB.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004193. Glyco_hydro_13_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    [Graphical view ]
    PANTHERi PTHR10357. PTHR10357. 1 hit.
    Pfami PF00128. Alpha-amylase. 1 hit.
    PF02806. Alpha-amylase_C. 1 hit.
    PF02922. CBM_48. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000463. GlgB. 1 hit.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF81296. SSF81296. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Starch branching enzyme II from maize endosperm."
      Fisher D.K., Boyer C.D., Hannah L.C.
      Plant Physiol. 102:1045-1046(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 58-65.
      Strain: cv. W64A X 182E.
      Tissue: Endosperm.
    2. "Expression of branching enzyme II of maize endosperm in Escherichia coli."
      Guan H.P., Baba T., Preiss J.
      Cell. Mol. Biol. 40:981-988(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 248-271 AND 305-315.
      Strain: cv. B73.
      Tissue: Endosperm.

    Entry informationi

    Entry nameiGLGB_MAIZE
    AccessioniPrimary (citable) accession number: Q08047
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3