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Protein

Cell division control protein 45

Gene

CDC45

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for initiation of chromosomal DNA replication. Acts at the origin of replication. Also has a role in minichromosome maintenance.2 Publications

GO - Molecular functioni

  • chromatin binding Source: SGD
  • DNA replication origin binding Source: SGD
  • single-stranded DNA binding Source: SGD

GO - Biological processi

  • DNA replication initiation Source: SGD
  • double-strand break repair via break-induced replication Source: SGD
  • pre-replicative complex assembly involved in nuclear cell cycle DNA replication Source: SGD
  • regulation of chromatin silencing at telomere Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, DNA replication

Enzyme and pathway databases

BioCyciYEAST:G3O-32251-MONOMER.
ReactomeiREACT_303130. Unwinding of DNA.
REACT_303865. E2F mediated regulation of DNA replication.
REACT_345909. G1/S-Specific Transcription.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division control protein 45
Gene namesi
Name:CDC45
Synonyms:SLD4
Ordered Locus Names:YLR103C
ORF Names:L8004.11
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XII

Organism-specific databases

CYGDiYLR103c.
EuPathDBiFungiDB:YLR103C.
SGDiS000004093. CDC45.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

  • DNA replication preinitiation complex Source: SGD
  • nuclear pre-replicative complex Source: SGD
  • nuclear replication fork Source: SGD
  • nucleus Source: SGD
  • replication fork protection complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 650650Cell division control protein 45PRO_0000192819Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei453 – 4531Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ08032.
PaxDbiQ08032.
PeptideAtlasiQ08032.

Interactioni

Subunit structurei

Assembles into a complex with MCM5/CDC46. Interacts with MCM10.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
MRC1P255883EBI-4292,EBI-412442
SLD3P531355EBI-4292,EBI-23925
TOF1P538403EBI-4292,EBI-28257

Protein-protein interaction databases

BioGridi31376. 141 interactions.
DIPiDIP-2405N.
IntActiQ08032. 34 interactions.
MINTiMINT-527915.

Structurei

3D structure databases

ProteinModelPortaliQ08032.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CDC45 family.Curated

Phylogenomic databases

eggNOGiNOG321683.
GeneTreeiENSGT00390000009662.
HOGENOMiHOG000248038.
InParanoidiQ08032.
KOiK06628.
OMAiALCACRI.
OrthoDBiEOG7H4F3M.

Family and domain databases

InterProiIPR003874. CDC45.
[Graphical view]
PANTHERiPTHR10507. PTHR10507. 1 hit.
PfamiPF02724. CDC45. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q08032-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYYGISQFSE AYNKILRNSS SHSSCQLVIF VSCLNIDALC ATKMLSLLFK
60 70 80 90 100
KQLVQSQIVP IFGYSELRRH YSQLDDNINS LLLVGFGGVI DLEAFLEIDP
110 120 130 140 150
QEYVIDTDEK SGEQSFRRDI YVLDAHRPWN LDNIFGSQII QCFDDGTVDD
160 170 180 190 200
TLGEQKEAYY KLLELDEESG DDELSGDEND NNGGDDEATD ADEVTDEDEE
210 220 230 240 250
DEDETISNKR GNSSIGPNDL SKRKQRKKQI HEYEGVLEEY YSQGTTVVNS
260 270 280 290 300
ISAQIYSLLS AIGETNLSNL WLNILGTTSL DIAYAQVYNR LYPLLQDEVK
310 320 330 340 350
RLTPSSRNSV KTPDTLTLNI QPDYYLFLLR HSSLYDSFYY SNYVNAKLSL
360 370 380 390 400
WNENGKKRLH KMFARMGIPL STAQETWLYM DHSIKRELGI IFDKNLDRYG
410 420 430 440 450
LQDIIRDGFV RTLGYRGSIS ASEFVEALTA LLEVGNSTDK DSVKINNDNN
460 470 480 490 500
DDTDGEEEED NSAQKLTNLR KRWVSNFWLS WDALDDRKVE LLNRGIQLAQ
510 520 530 540 550
DLQRAIFNTG VAILEKKLIK HLRIYRLCVL QDGPDLDLYR NPLTLLRLGN
560 570 580 590 600
WLIECCAESE DKQLLPMVLA SIDENTDTYL VAGLTPRYPR GLDTIHTKKP
610 620 630 640 650
ILNNFSMAFQ QITAETDAKV RIDNFESSII EIRREDLSPF LEKLTLSGLL
Length:650
Mass (Da):74,249
Last modified:November 1, 1996 - v1
Checksum:i6500DDD62EC4E8EE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti576 – 5761T → K in AAB67546 (PubMed:9169871).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U65790 Genomic DNA. Translation: AAC49620.1.
U56821 Genomic DNA. Translation: AAB09053.1.
Z73275 Genomic DNA. Translation: CAA97668.1.
U53876 Genomic DNA. Translation: AAB67546.1.
BK006945 Genomic DNA. Translation: DAA09419.1.
PIRiS64939.
RefSeqiNP_013204.1. NM_001181990.1.

Genome annotation databases

EnsemblFungiiYLR103C; YLR103C; YLR103C.
GeneIDi850793.
KEGGisce:YLR103C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U65790 Genomic DNA. Translation: AAC49620.1.
U56821 Genomic DNA. Translation: AAB09053.1.
Z73275 Genomic DNA. Translation: CAA97668.1.
U53876 Genomic DNA. Translation: AAB67546.1.
BK006945 Genomic DNA. Translation: DAA09419.1.
PIRiS64939.
RefSeqiNP_013204.1. NM_001181990.1.

3D structure databases

ProteinModelPortaliQ08032.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31376. 141 interactions.
DIPiDIP-2405N.
IntActiQ08032. 34 interactions.
MINTiMINT-527915.

Proteomic databases

MaxQBiQ08032.
PaxDbiQ08032.
PeptideAtlasiQ08032.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR103C; YLR103C; YLR103C.
GeneIDi850793.
KEGGisce:YLR103C.

Organism-specific databases

CYGDiYLR103c.
EuPathDBiFungiDB:YLR103C.
SGDiS000004093. CDC45.

Phylogenomic databases

eggNOGiNOG321683.
GeneTreeiENSGT00390000009662.
HOGENOMiHOG000248038.
InParanoidiQ08032.
KOiK06628.
OMAiALCACRI.
OrthoDBiEOG7H4F3M.

Enzyme and pathway databases

BioCyciYEAST:G3O-32251-MONOMER.
ReactomeiREACT_303130. Unwinding of DNA.
REACT_303865. E2F mediated regulation of DNA replication.
REACT_345909. G1/S-Specific Transcription.

Miscellaneous databases

NextBioi967002.
PROiQ08032.

Family and domain databases

InterProiIPR003874. CDC45.
[Graphical view]
PANTHERiPTHR10507. PTHR10507. 1 hit.
PfamiPF02724. CDC45. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "CDC45, a novel yeast gene that functions with the origin recognition complex and Mcm proteins in initiation of DNA replication."
    Zou L., Mitchell J., Stillman B.
    Mol. Cell. Biol. 17:553-563(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
  2. "Cdc45p assembles into a complex with Cdc46p/Mcm5p, is required for minichromosome maintenance, and is essential for chromosomal DNA replication."
    Hopwood B., Dalton S.
    Proc. Natl. Acad. Sci. U.S.A. 93:12309-12314(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
  3. Messenguy F., Dubois E., Vierendeels F., Scherens B.
    Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Mcm10 and Cdc45 cooperate in origin activation in Saccharomyces cerevisiae."
    Sawyer S.L., Cheng I.H., Chai W., Tye B.K.
    J. Mol. Biol. 340:195-202(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MCM10.
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-453, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCDC45_YEAST
AccessioniPrimary (citable) accession number: Q08032
Secondary accession number(s): D6VYA3, Q05908
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: July 22, 2015
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1730 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.