Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q08032

- CDC45_YEAST

UniProt

Q08032 - CDC45_YEAST

Protein

Cell division control protein 45

Gene

CDC45

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Required for initiation of chromosomal DNA replication. Acts at the origin of replication. Also has a role in minichromosome maintenance.2 Publications

    GO - Molecular functioni

    1. chromatin binding Source: SGD
    2. DNA replication origin binding Source: SGD
    3. protein binding Source: IntAct
    4. single-stranded DNA binding Source: SGD

    GO - Biological processi

    1. DNA replication initiation Source: SGD
    2. double-strand break repair via break-induced replication Source: SGD
    3. pre-replicative complex assembly involved in nuclear cell cycle DNA replication Source: SGD
    4. regulation of chromatin silencing at telomere Source: SGD

    Keywords - Biological processi

    Cell cycle, DNA replication

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32251-MONOMER.
    ReactomeiREACT_189247. G1/S-Specific Transcription.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cell division control protein 45
    Gene namesi
    Name:CDC45
    Synonyms:SLD4
    Ordered Locus Names:YLR103C
    ORF Names:L8004.11
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    CYGDiYLR103c.
    SGDiS000004093. CDC45.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. DNA replication preinitiation complex Source: SGD
    2. nuclear pre-replicative complex Source: SGD
    3. nuclear replication fork Source: SGD
    4. nucleus Source: SGD
    5. replication fork protection complex Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 650650Cell division control protein 45PRO_0000192819Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei453 – 4531Phosphothreonine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ08032.
    PaxDbiQ08032.
    PeptideAtlasiQ08032.

    Expressioni

    Gene expression databases

    GenevestigatoriQ08032.

    Interactioni

    Subunit structurei

    Assembles into a complex with MCM5/CDC46. Interacts with MCM10.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    MRC1P255883EBI-4292,EBI-412442
    SLD3P531355EBI-4292,EBI-23925
    TOF1P538403EBI-4292,EBI-28257

    Protein-protein interaction databases

    BioGridi31376. 141 interactions.
    DIPiDIP-2405N.
    IntActiQ08032. 34 interactions.
    MINTiMINT-527915.

    Structurei

    3D structure databases

    ProteinModelPortaliQ08032.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the CDC45 family.Curated

    Phylogenomic databases

    eggNOGiNOG321683.
    GeneTreeiENSGT00390000009662.
    HOGENOMiHOG000248038.
    KOiK06628.
    OMAiALCACRI.
    OrthoDBiEOG7H4F3M.

    Family and domain databases

    InterProiIPR003874. CDC45.
    [Graphical view]
    PANTHERiPTHR10507. PTHR10507. 1 hit.
    PfamiPF02724. CDC45. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q08032-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYYGISQFSE AYNKILRNSS SHSSCQLVIF VSCLNIDALC ATKMLSLLFK    50
    KQLVQSQIVP IFGYSELRRH YSQLDDNINS LLLVGFGGVI DLEAFLEIDP 100
    QEYVIDTDEK SGEQSFRRDI YVLDAHRPWN LDNIFGSQII QCFDDGTVDD 150
    TLGEQKEAYY KLLELDEESG DDELSGDEND NNGGDDEATD ADEVTDEDEE 200
    DEDETISNKR GNSSIGPNDL SKRKQRKKQI HEYEGVLEEY YSQGTTVVNS 250
    ISAQIYSLLS AIGETNLSNL WLNILGTTSL DIAYAQVYNR LYPLLQDEVK 300
    RLTPSSRNSV KTPDTLTLNI QPDYYLFLLR HSSLYDSFYY SNYVNAKLSL 350
    WNENGKKRLH KMFARMGIPL STAQETWLYM DHSIKRELGI IFDKNLDRYG 400
    LQDIIRDGFV RTLGYRGSIS ASEFVEALTA LLEVGNSTDK DSVKINNDNN 450
    DDTDGEEEED NSAQKLTNLR KRWVSNFWLS WDALDDRKVE LLNRGIQLAQ 500
    DLQRAIFNTG VAILEKKLIK HLRIYRLCVL QDGPDLDLYR NPLTLLRLGN 550
    WLIECCAESE DKQLLPMVLA SIDENTDTYL VAGLTPRYPR GLDTIHTKKP 600
    ILNNFSMAFQ QITAETDAKV RIDNFESSII EIRREDLSPF LEKLTLSGLL 650
    Length:650
    Mass (Da):74,249
    Last modified:November 1, 1996 - v1
    Checksum:i6500DDD62EC4E8EE
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti576 – 5761T → K in AAB67546. (PubMed:9169871)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U65790 Genomic DNA. Translation: AAC49620.1.
    U56821 Genomic DNA. Translation: AAB09053.1.
    Z73275 Genomic DNA. Translation: CAA97668.1.
    U53876 Genomic DNA. Translation: AAB67546.1.
    BK006945 Genomic DNA. Translation: DAA09419.1.
    PIRiS64939.
    RefSeqiNP_013204.1. NM_001181990.1.

    Genome annotation databases

    EnsemblFungiiYLR103C; YLR103C; YLR103C.
    GeneIDi850793.
    KEGGisce:YLR103C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U65790 Genomic DNA. Translation: AAC49620.1 .
    U56821 Genomic DNA. Translation: AAB09053.1 .
    Z73275 Genomic DNA. Translation: CAA97668.1 .
    U53876 Genomic DNA. Translation: AAB67546.1 .
    BK006945 Genomic DNA. Translation: DAA09419.1 .
    PIRi S64939.
    RefSeqi NP_013204.1. NM_001181990.1.

    3D structure databases

    ProteinModelPortali Q08032.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31376. 141 interactions.
    DIPi DIP-2405N.
    IntActi Q08032. 34 interactions.
    MINTi MINT-527915.

    Proteomic databases

    MaxQBi Q08032.
    PaxDbi Q08032.
    PeptideAtlasi Q08032.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLR103C ; YLR103C ; YLR103C .
    GeneIDi 850793.
    KEGGi sce:YLR103C.

    Organism-specific databases

    CYGDi YLR103c.
    SGDi S000004093. CDC45.

    Phylogenomic databases

    eggNOGi NOG321683.
    GeneTreei ENSGT00390000009662.
    HOGENOMi HOG000248038.
    KOi K06628.
    OMAi ALCACRI.
    OrthoDBi EOG7H4F3M.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32251-MONOMER.
    Reactomei REACT_189247. G1/S-Specific Transcription.

    Miscellaneous databases

    NextBioi 967002.
    PROi Q08032.

    Gene expression databases

    Genevestigatori Q08032.

    Family and domain databases

    InterProi IPR003874. CDC45.
    [Graphical view ]
    PANTHERi PTHR10507. PTHR10507. 1 hit.
    Pfami PF02724. CDC45. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "CDC45, a novel yeast gene that functions with the origin recognition complex and Mcm proteins in initiation of DNA replication."
      Zou L., Mitchell J., Stillman B.
      Mol. Cell. Biol. 17:553-563(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    2. "Cdc45p assembles into a complex with Cdc46p/Mcm5p, is required for minichromosome maintenance, and is essential for chromosomal DNA replication."
      Hopwood B., Dalton S.
      Proc. Natl. Acad. Sci. U.S.A. 93:12309-12314(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
    3. Messenguy F., Dubois E., Vierendeels F., Scherens B.
      Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Mcm10 and Cdc45 cooperate in origin activation in Saccharomyces cerevisiae."
      Sawyer S.L., Cheng I.H., Chai W., Tye B.K.
      J. Mol. Biol. 340:195-202(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MCM10.
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-453, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCDC45_YEAST
    AccessioniPrimary (citable) accession number: Q08032
    Secondary accession number(s): D6VYA3, Q05908
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 120 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1730 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3