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Q08032 (CDC45_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cell division control protein 45
Gene names
Name:CDC45
Synonyms:SLD4
Ordered Locus Names:YLR103C
ORF Names:L8004.11
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length650 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for initiation of chromosomal DNA replication. Acts at the origin of replication. Also has a role in minichromosome maintenance. Ref.1 Ref.2

Subunit structure

Assembles into a complex with MCM5/CDC46. Interacts with MCM10. Ref.7

Subcellular location

Nucleus Ref.2.

Miscellaneous

Present with 1730 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the CDC45 family.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 650650Cell division control protein 45
PRO_0000192819

Amino acid modifications

Modified residue4531Phosphothreonine Ref.8

Experimental info

Sequence conflict5761T → K in AAB67546. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q08032 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 6500DDD62EC4E8EE

FASTA65074,249
        10         20         30         40         50         60 
MYYGISQFSE AYNKILRNSS SHSSCQLVIF VSCLNIDALC ATKMLSLLFK KQLVQSQIVP 

        70         80         90        100        110        120 
IFGYSELRRH YSQLDDNINS LLLVGFGGVI DLEAFLEIDP QEYVIDTDEK SGEQSFRRDI 

       130        140        150        160        170        180 
YVLDAHRPWN LDNIFGSQII QCFDDGTVDD TLGEQKEAYY KLLELDEESG DDELSGDEND 

       190        200        210        220        230        240 
NNGGDDEATD ADEVTDEDEE DEDETISNKR GNSSIGPNDL SKRKQRKKQI HEYEGVLEEY 

       250        260        270        280        290        300 
YSQGTTVVNS ISAQIYSLLS AIGETNLSNL WLNILGTTSL DIAYAQVYNR LYPLLQDEVK 

       310        320        330        340        350        360 
RLTPSSRNSV KTPDTLTLNI QPDYYLFLLR HSSLYDSFYY SNYVNAKLSL WNENGKKRLH 

       370        380        390        400        410        420 
KMFARMGIPL STAQETWLYM DHSIKRELGI IFDKNLDRYG LQDIIRDGFV RTLGYRGSIS 

       430        440        450        460        470        480 
ASEFVEALTA LLEVGNSTDK DSVKINNDNN DDTDGEEEED NSAQKLTNLR KRWVSNFWLS 

       490        500        510        520        530        540 
WDALDDRKVE LLNRGIQLAQ DLQRAIFNTG VAILEKKLIK HLRIYRLCVL QDGPDLDLYR 

       550        560        570        580        590        600 
NPLTLLRLGN WLIECCAESE DKQLLPMVLA SIDENTDTYL VAGLTPRYPR GLDTIHTKKP 

       610        620        630        640        650 
ILNNFSMAFQ QITAETDAKV RIDNFESSII EIRREDLSPF LEKLTLSGLL 

« Hide

References

« Hide 'large scale' references
[1]"CDC45, a novel yeast gene that functions with the origin recognition complex and Mcm proteins in initiation of DNA replication."
Zou L., Mitchell J., Stillman B.
Mol. Cell. Biol. 17:553-563(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]"Cdc45p assembles into a complex with Cdc46p/Mcm5p, is required for minichromosome maintenance, and is essential for chromosomal DNA replication."
Hopwood B., Dalton S.
Proc. Natl. Acad. Sci. U.S.A. 93:12309-12314(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
[3]Messenguy F., Dubois E., Vierendeels F., Scherens B.
Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. expand/collapse author list , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Mcm10 and Cdc45 cooperate in origin activation in Saccharomyces cerevisiae."
Sawyer S.L., Cheng I.H., Chai W., Tye B.K.
J. Mol. Biol. 340:195-202(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MCM10.
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-453, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U65790 Genomic DNA. Translation: AAC49620.1.
U56821 Genomic DNA. Translation: AAB09053.1.
Z73275 Genomic DNA. Translation: CAA97668.1.
U53876 Genomic DNA. Translation: AAB67546.1.
BK006945 Genomic DNA. Translation: DAA09419.1.
PIRS64939.
RefSeqNP_013204.1. NM_001181990.1.

3D structure databases

ProteinModelPortalQ08032.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31376. 140 interactions.
DIPDIP-2405N.
IntActQ08032. 34 interactions.
MINTMINT-527915.

Proteomic databases

MaxQBQ08032.
PaxDbQ08032.
PeptideAtlasQ08032.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYLR103C; YLR103C; YLR103C.
GeneID850793.
KEGGsce:YLR103C.

Organism-specific databases

CYGDYLR103c.
SGDS000004093. CDC45.

Phylogenomic databases

eggNOGNOG321683.
GeneTreeENSGT00390000009662.
HOGENOMHOG000248038.
KOK06628.
OMAALCACRI.
OrthoDBEOG7H4F3M.

Enzyme and pathway databases

BioCycYEAST:G3O-32251-MONOMER.

Gene expression databases

GenevestigatorQ08032.

Family and domain databases

InterProIPR003874. CDC45.
[Graphical view]
PANTHERPTHR10507. PTHR10507. 1 hit.
PfamPF02724. CDC45. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio967002.
PROQ08032.

Entry information

Entry nameCDC45_YEAST
AccessionPrimary (citable) accession number: Q08032
Secondary accession number(s): D6VYA3, Q05908
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families