Cell division control protein 45 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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Q08032 (CDC45_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 105. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Cell division control protein 45

Gene names

Name:	CDC45
Synonyms:	SLD4
Ordered Locus Names:	YLR103C
ORF Names:	L8004.11

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	650 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for initiation of chromosomal DNA replication. Acts at the origin of replication. Also has a role in minichromosome maintenance. Ref.1 Ref.2
Subunit structure	Assembles into a complex with MCM5/CDC46. Interacts with MCM10. Ref.7
Subcellular location	Nucleus Ref.2.
Miscellaneous	Present with 1730 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the CDC45 family.

Ontologies

Keywords
Biological process	Cell cycle DNA replication
Cellular component	Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication initiation Inferred from mutant phenotype PubMed 2044962. Source: SGD S phase of mitotic cell cycle Inferred from mutant phenotype PubMed 2044962. Source: SGD double-strand break repair via break-induced replication Inferred from mutant phenotype PubMed 20516198. Source: SGD pre-replicative complex assembly Inferred from physical interaction PubMed 9335335. Source: SGD regulation of chromatin silencing at telomere Inferred from mutant phenotype PubMed 16980387. Source: SGD
Cellular_component	DNA replication preinitiation complex Inferred from direct assay PubMed 9554851. Source: SGD pre-replicative complex Inferred from direct assay PubMed 9335335. Source: SGD replication fork protection complex Inferred from direct assay PubMed 16531994. Source: SGD
Molecular_function	DNA replication origin binding Inferred from direct assay PubMed 12019222. Source: SGD chromatin binding Inferred from direct assay PubMed 15494305 PubMed 9554851. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MRC1	P25588	3	EBI-4292,EBI-412442
TOF1	P53840	3	EBI-4292,EBI-28257

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 650

650

Cell division control protein 45

PRO_0000192819

Amino acid modifications

Modified residue

213

Phosphoserine Ref.8

Modified residue

214

Phosphoserine Ref.8

Modified residue

453

Phosphothreonine Ref.8

Experimental info

Sequence conflict

576

T → K in AAB67546. Ref.4

Sequences

Sequence

Length

Mass (Da)

Tools

Q08032 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 6500DDD62EC4E8EE
Show »

FASTA

650

74,249

        10         20         30         40         50         60 
MYYGISQFSE AYNKILRNSS SHSSCQLVIF VSCLNIDALC ATKMLSLLFK KQLVQSQIVP 

        70         80         90        100        110        120 
IFGYSELRRH YSQLDDNINS LLLVGFGGVI DLEAFLEIDP QEYVIDTDEK SGEQSFRRDI 

       130        140        150        160        170        180 
YVLDAHRPWN LDNIFGSQII QCFDDGTVDD TLGEQKEAYY KLLELDEESG DDELSGDEND 

       190        200        210        220        230        240 
NNGGDDEATD ADEVTDEDEE DEDETISNKR GNSSIGPNDL SKRKQRKKQI HEYEGVLEEY 

       250        260        270        280        290        300 
YSQGTTVVNS ISAQIYSLLS AIGETNLSNL WLNILGTTSL DIAYAQVYNR LYPLLQDEVK 

       310        320        330        340        350        360 
RLTPSSRNSV KTPDTLTLNI QPDYYLFLLR HSSLYDSFYY SNYVNAKLSL WNENGKKRLH 

       370        380        390        400        410        420 
KMFARMGIPL STAQETWLYM DHSIKRELGI IFDKNLDRYG LQDIIRDGFV RTLGYRGSIS 

       430        440        450        460        470        480 
ASEFVEALTA LLEVGNSTDK DSVKINNDNN DDTDGEEEED NSAQKLTNLR KRWVSNFWLS 

       490        500        510        520        530        540 
WDALDDRKVE LLNRGIQLAQ DLQRAIFNTG VAILEKKLIK HLRIYRLCVL QDGPDLDLYR 

       550        560        570        580        590        600 
NPLTLLRLGN WLIECCAESE DKQLLPMVLA SIDENTDTYL VAGLTPRYPR GLDTIHTKKP 

       610        620        630        640        650 
ILNNFSMAFQ QITAETDAKV RIDNFESSII EIRREDLSPF LEKLTLSGLL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"CDC45, a novel yeast gene that functions with the origin recognition complex and Mcm proteins in initiation of DNA replication." Zou L., Mitchell J., Stillman B. Mol. Cell. Biol. 17:553-563(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]	"Cdc45p assembles into a complex with Cdc46p/Mcm5p, is required for minichromosome maintenance, and is essential for chromosomal DNA replication." Hopwood B., Dalton S. Proc. Natl. Acad. Sci. U.S.A. 93:12309-12314(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
[3]	Messenguy F., Dubois E., Vierendeels F., Scherens B. Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. Hoheisel J.D. Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Mcm10 and Cdc45 cooperate in origin activation in Saccharomyces cerevisiae." Sawyer S.L., Cheng I.H., Chai W., Tye B.K. J. Mol. Biol. 340:195-202(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH MCM10.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-213; SER-214 AND THR-453, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U65790 Genomic DNA. Translation: AAC49620.1. U56821 Genomic DNA. Translation: AAB09053.1. Z73275 Genomic DNA. Translation: CAA97668.1. U53876 Genomic DNA. Translation: AAB67546.1. BK006945 Genomic DNA. Translation: DAA09419.1.
PIR	S64939.
RefSeq	NP_013204.1. NM_001181990.1.
3D structure databases
ProteinModelPortal	Q08032.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2405N.
IntAct	Q08032. 29 interactions.
MINT	MINT-527915.
Proteomic databases
PaxDb	Q08032.
PeptideAtlas	Q08032.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR103C; YLR103C; YLR103C.
GeneID	850793.
KEGG	sce:YLR103C.
Organism-specific databases
CYGD	YLR103c.
SGD	S000004093. CDC45.
Phylogenomic databases
eggNOG	NOG321683.
GeneTree	ENSGT00390000009662.
HOGENOM	HOG000248038.
KO	K06628.
OMA	MLHSPYL.
OrthoDB	EOG41VPB8.
Gene expression databases
Genevestigator	Q08032.
GermOnline	YLR103C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003874. CDC45. [Graphical view]
PANTHER	PTHR10507. PTHR10507. 1 hit.
Pfam	PF02724. CDC45. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	967002.

Entry information

Entry name

CDC45_YEAST

Accession

Primary (citable) accession number: Q08032
Secondary accession number(s): D6VYA3, Q05908

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents