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Protein

Core-binding factor subunit beta

Gene

Cbfb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. CBFB enhances DNA binding by RUNX1.

GO - Molecular functioni

GO - Biological processi

  • cell maturation Source: MGI
  • definitive hemopoiesis Source: MGI
  • lymphocyte differentiation Source: MGI
  • myeloid cell differentiation Source: MGI
  • ossification Source: MGI
  • osteoblast differentiation Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • protein polyubiquitination Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Core-binding factor subunit beta
Short name:
CBF-beta
Alternative name(s):
Polyomavirus enhancer-binding protein 2 beta subunit
Short name:
PEA2-beta
Short name:
PEBP2-beta
SL3-3 enhancer factor 1 subunit beta
SL3/AKV core-binding factor beta subunit
Gene namesi
Name:Cbfb
Synonyms:Pebp2b, Pebpb2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:99851. Cbfb.

Subcellular locationi

GO - Cellular componenti

  • core-binding factor complex Source: GO_Central
  • membrane Source: MGI
  • nucleus Source: MGI
  • protein complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi5V → A: Interferes with heterodimerization. 1 Publication1
Mutagenesisi63N → A: Interferes with heterodimerization. 1 Publication1
Mutagenesisi104N → A: Interferes with heterodimerization. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000583021 – 187Core-binding factor subunit betaAdd BLAST187

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei10Phosphoserine; by CK2Sequence analysis1
Modified residuei159Phosphoserine; by PKCSequence analysis1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ08024.
MaxQBiQ08024.
PaxDbiQ08024.
PeptideAtlasiQ08024.
PRIDEiQ08024.

PTM databases

iPTMnetiQ08024.
PhosphoSitePlusiQ08024.

Expressioni

Tissue specificityi

Expressed in all tissues tested. Highest level in thymus, but also abundantly expressed in muscle, lung and brain.

Gene expression databases

BgeeiENSMUSG00000031885.
CleanExiMM_CBFB.
GenevisibleiQ08024. MM.

Interactioni

Subunit structurei

Heterodimer with RUNX1. Interacts with COPRS. Found in a complex with PRMT5, RUNX1 and CBFB.2 Publications

Protein-protein interaction databases

BioGridi198525. 5 interactors.
IntActiQ08024. 3 interactors.
STRINGi10090.ENSMUSP00000059382.

Structurei

Secondary structure

1187
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 12Combined sources5
Helixi16 – 19Combined sources4
Turni20 – 22Combined sources3
Beta strandi25 – 29Combined sources5
Turni31 – 34Combined sources4
Helixi37 – 49Combined sources13
Beta strandi52 – 57Combined sources6
Turni58 – 61Combined sources4
Beta strandi62 – 67Combined sources6
Beta strandi83 – 86Combined sources4
Beta strandi88 – 103Combined sources16
Beta strandi106 – 115Combined sources10
Turni116 – 118Combined sources3
Beta strandi120 – 127Combined sources8
Helixi129 – 139Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ILFNMR-A1-141[»]
1IO4X-ray3.00D1-141[»]
2JHBNMR-A1-141[»]
3WTSX-ray2.35B/G1-142[»]
3WTTX-ray2.35B/G1-142[»]
3WTUX-ray2.70B/G1-142[»]
3WTVX-ray2.70B/G1-142[»]
3WTWX-ray2.90B/G1-142[»]
3WTXX-ray2.80B/G1-142[»]
3WTYX-ray2.70B/G1-142[»]
ProteinModelPortaliQ08024.
SMRiQ08024.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ08024.

Family & Domainsi

Sequence similaritiesi

Belongs to the CBF-beta family.Curated

Phylogenomic databases

eggNOGiKOG4785. Eukaryota.
ENOG410Z04Q. LUCA.
GeneTreeiENSGT00390000018132.
HOGENOMiHOG000008040.
HOVERGENiHBG000541.
InParanoidiQ08024.
OMAiGYLEYDD.
OrthoDBiEOG091G0VFL.
PhylomeDBiQ08024.
TreeFamiTF314675.

Family and domain databases

Gene3Di2.40.250.10. 1 hit.
InterProiIPR003417. CBF_beta.
[Graphical view]
PANTHERiPTHR10276. PTHR10276. 1 hit.
PfamiPF02312. CBF_beta. 1 hit.
[Graphical view]
SUPFAMiSSF50723. SSF50723. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q08024-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPRVVPDQRS KFENEEFFRK LSRECEIKYT GFRDRPHEER QTRFQNACRD
60 70 80 90 100
GRSEIAFVAT GTNLSLQFFP ASWQGEQRQT PSREYVDLER EAGKVYLKAP
110 120 130 140 150
MILNGVCVIW KGWIDLHRLD GMGCLEFDEE RAQQEDALAQ QAFEEARRRT
160 170 180
REFEDRDRSH REEMEARRQQ DPSPGSNLGG GDDLKLR
Length:187
Mass (Da):22,030
Last modified:November 1, 1996 - v1
Checksum:i0B2E6101A35D0FD8
GO
Isoform 2 (identifier: Q08024-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     166-187: ARRQQDPSPGSNLGGGDDLKLR → VRVSQLLAVTGKKTARP

Note: Major isoform.
Show »
Length:182
Mass (Da):21,517
Checksum:i20EC3C81F39874FA
GO
Isoform 3 (identifier: Q08024-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     134-165: Missing.

Note: Does not dimerize with the alpha subunit.
Show »
Length:155
Mass (Da):18,026
Checksum:i1E4C0F13B9B25697
GO
Isoform 4 (identifier: Q08024-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     56-94: Missing.

Note: Does not dimerize with the alpha subunit.
Show »
Length:148
Mass (Da):17,633
Checksum:i4265D3ADB61D1352
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2P → S in BAC40748 (PubMed:16141072).Curated1
Sequence conflicti40R → P in BAC40748 (PubMed:16141072).Curated1
Sequence conflicti171D → N in L03279 (PubMed:8497254).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00435856 – 94Missing in isoform 4. 1 PublicationAdd BLAST39
Alternative sequenceiVSP_004359134 – 165Missing in isoform 3. 1 PublicationAdd BLAST32
Alternative sequenceiVSP_004360166 – 187ARRQQ…DLKLR → VRVSQLLAVTGKKTARP in isoform 2. 2 PublicationsAdd BLAST22

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14572 mRNA. Translation: BAA03426.1.
D14571 mRNA. Translation: BAA03425.1.
D14570 mRNA. Translation: BAA03424.1.
D14569 Genomic DNA. Translation: BAA03423.1.
L03305 mRNA. No translation available.
L03306 mRNA. No translation available.
L03279 mRNA. No translation available.
AK089094 mRNA. Translation: BAC40748.1.
BC006763 mRNA. Translation: AAH06763.1.
BC026749 mRNA. Translation: AAH26749.1.
BC040752 mRNA. Translation: AAH40752.2.
CCDSiCCDS22592.1. [Q08024-1]
CCDS52654.1. [Q08024-2]
CCDS52655.1. [Q08024-3]
CCDS52656.1. [Q08024-4]
PIRiA46107.
B48124.
RefSeqiNP_001154928.1. NM_001161456.1. [Q08024-4]
NP_001154929.1. NM_001161457.1. [Q08024-3]
NP_001154930.1. NM_001161458.1. [Q08024-2]
NP_071704.3. NM_022309.4. [Q08024-1]
UniGeneiMm.2018.

Genome annotation databases

EnsembliENSMUST00000052209; ENSMUSP00000059382; ENSMUSG00000031885. [Q08024-1]
ENSMUST00000109392; ENSMUSP00000105019; ENSMUSG00000031885. [Q08024-2]
ENSMUST00000109394; ENSMUSP00000105021; ENSMUSG00000031885. [Q08024-4]
ENSMUST00000109395; ENSMUSP00000105022; ENSMUSG00000031885. [Q08024-3]
GeneIDi12400.
KEGGimmu:12400.
UCSCiuc009nbp.2. mouse. [Q08024-1]
uc009nbq.2. mouse. [Q08024-2]
uc009nbr.2. mouse. [Q08024-4]
uc012gjd.1. mouse. [Q08024-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14572 mRNA. Translation: BAA03426.1.
D14571 mRNA. Translation: BAA03425.1.
D14570 mRNA. Translation: BAA03424.1.
D14569 Genomic DNA. Translation: BAA03423.1.
L03305 mRNA. No translation available.
L03306 mRNA. No translation available.
L03279 mRNA. No translation available.
AK089094 mRNA. Translation: BAC40748.1.
BC006763 mRNA. Translation: AAH06763.1.
BC026749 mRNA. Translation: AAH26749.1.
BC040752 mRNA. Translation: AAH40752.2.
CCDSiCCDS22592.1. [Q08024-1]
CCDS52654.1. [Q08024-2]
CCDS52655.1. [Q08024-3]
CCDS52656.1. [Q08024-4]
PIRiA46107.
B48124.
RefSeqiNP_001154928.1. NM_001161456.1. [Q08024-4]
NP_001154929.1. NM_001161457.1. [Q08024-3]
NP_001154930.1. NM_001161458.1. [Q08024-2]
NP_071704.3. NM_022309.4. [Q08024-1]
UniGeneiMm.2018.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ILFNMR-A1-141[»]
1IO4X-ray3.00D1-141[»]
2JHBNMR-A1-141[»]
3WTSX-ray2.35B/G1-142[»]
3WTTX-ray2.35B/G1-142[»]
3WTUX-ray2.70B/G1-142[»]
3WTVX-ray2.70B/G1-142[»]
3WTWX-ray2.90B/G1-142[»]
3WTXX-ray2.80B/G1-142[»]
3WTYX-ray2.70B/G1-142[»]
ProteinModelPortaliQ08024.
SMRiQ08024.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198525. 5 interactors.
IntActiQ08024. 3 interactors.
STRINGi10090.ENSMUSP00000059382.

PTM databases

iPTMnetiQ08024.
PhosphoSitePlusiQ08024.

Proteomic databases

EPDiQ08024.
MaxQBiQ08024.
PaxDbiQ08024.
PeptideAtlasiQ08024.
PRIDEiQ08024.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000052209; ENSMUSP00000059382; ENSMUSG00000031885. [Q08024-1]
ENSMUST00000109392; ENSMUSP00000105019; ENSMUSG00000031885. [Q08024-2]
ENSMUST00000109394; ENSMUSP00000105021; ENSMUSG00000031885. [Q08024-4]
ENSMUST00000109395; ENSMUSP00000105022; ENSMUSG00000031885. [Q08024-3]
GeneIDi12400.
KEGGimmu:12400.
UCSCiuc009nbp.2. mouse. [Q08024-1]
uc009nbq.2. mouse. [Q08024-2]
uc009nbr.2. mouse. [Q08024-4]
uc012gjd.1. mouse. [Q08024-3]

Organism-specific databases

CTDi865.
MGIiMGI:99851. Cbfb.

Phylogenomic databases

eggNOGiKOG4785. Eukaryota.
ENOG410Z04Q. LUCA.
GeneTreeiENSGT00390000018132.
HOGENOMiHOG000008040.
HOVERGENiHBG000541.
InParanoidiQ08024.
OMAiGYLEYDD.
OrthoDBiEOG091G0VFL.
PhylomeDBiQ08024.
TreeFamiTF314675.

Miscellaneous databases

ChiTaRSiCbfb. mouse.
EvolutionaryTraceiQ08024.
PROiQ08024.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031885.
CleanExiMM_CBFB.
GenevisibleiQ08024. MM.

Family and domain databases

Gene3Di2.40.250.10. 1 hit.
InterProiIPR003417. CBF_beta.
[Graphical view]
PANTHERiPTHR10276. PTHR10276. 1 hit.
PfamiPF02312. CBF_beta. 1 hit.
[Graphical view]
SUPFAMiSSF50723. SSF50723. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPEBB_MOUSE
AccessioniPrimary (citable) accession number: Q08024
Secondary accession number(s): Q08025
, Q62050, Q62051, Q8C282, Q8CGD5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.