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Protein

Protein enhancer of sevenless 2B

Gene

drk

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for proper signaling by sevenless. May act to stimulate the ability of Sos to catalyze Ras1 activation by linking sevenless and Sos in a signaling complex.2 Publications

GO - Molecular functioni

  • non-membrane spanning protein tyrosine kinase activity Source: GO_Central
  • sevenless binding Source: FlyBase
  • SH3/SH2 adaptor activity Source: FlyBase
  • signal transducer activity Source: UniProtKB-KW

GO - Biological processi

  • activation of JNKK activity Source: UniProtKB
  • anterior/posterior axis specification, embryo Source: FlyBase
  • associative learning Source: FlyBase
  • cell migration Source: GO_Central
  • embryonic development via the syncytial blastoderm Source: FlyBase
  • imaginal disc-derived wing morphogenesis Source: FlyBase
  • innate immune response Source: GO_Central
  • negative regulation of epidermal growth factor-activated receptor activity Source: FlyBase
  • olfactory learning Source: FlyBase
  • ovarian follicle cell development Source: FlyBase
  • peptidyl-tyrosine autophosphorylation Source: GO_Central
  • positive regulation of MAPK cascade Source: FlyBase
  • positive regulation of R7 cell differentiation Source: FlyBase
  • Ras protein signal transduction Source: FlyBase
  • regulation of cell proliferation Source: GO_Central
  • sevenless signaling pathway Source: FlyBase
  • short-term memory Source: FlyBase
  • TORC1 signaling Source: FlyBase
  • torso signaling pathway Source: FlyBase

Keywordsi

Molecular functionTransducer

Enzyme and pathway databases

ReactomeiR-DME-109704 PI3K Cascade
R-DME-112412 SOS-mediated signalling
R-DME-1257604 PIP3 activates AKT signaling
R-DME-1295596 Spry regulation of FGF signaling
R-DME-1433557 Signaling by SCF-KIT
R-DME-1433559 Regulation of KIT signaling
R-DME-179812 GRB2 events in EGFR signaling
R-DME-180292 GAB1 signalosome
R-DME-180336 SHC1 events in EGFR signaling
R-DME-182971 EGFR downregulation
R-DME-1963640 GRB2 events in ERBB2 signaling
R-DME-1963642 PI3K events in ERBB2 signaling
R-DME-2029482 Regulation of actin dynamics for phagocytic cup formation
R-DME-210993 Tie2 Signaling
R-DME-2179392 EGFR Transactivation by Gastrin
R-DME-354194 GRB2:SOS provides linkage to MAPK signaling for Integrins
R-DME-375165 NCAM signaling for neurite out-growth
R-DME-5654689 PI-3K cascade:FGFR1
R-DME-5654693 FRS-mediated FGFR1 signaling
R-DME-5654695 PI-3K cascade:FGFR2
R-DME-5654699 SHC-mediated cascade:FGFR2
R-DME-5654700 FRS-mediated FGFR2 signaling
R-DME-5654704 SHC-mediated cascade:FGFR3
R-DME-5654706 FRS-mediated FGFR3 signaling
R-DME-5654710 PI-3K cascade:FGFR3
R-DME-5654712 FRS-mediated FGFR4 signaling
R-DME-5654720 PI-3K cascade:FGFR4
R-DME-5654726 Negative regulation of FGFR1 signaling
R-DME-5654727 Negative regulation of FGFR2 signaling
R-DME-5654732 Negative regulation of FGFR3 signaling
R-DME-5654733 Negative regulation of FGFR4 signaling
R-DME-5663213 RHO GTPases Activate WASPs and WAVEs
R-DME-5673001 RAF/MAP kinase cascade
R-DME-6811558 PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling
R-DME-74749 Signal attenuation
R-DME-74751 Insulin receptor signalling cascade
R-DME-8856825 Cargo recognition for clathrin-mediated endocytosis
R-DME-8856828 Clathrin-mediated endocytosis
R-DME-912631 Regulation of signaling by CBL
R-DME-983695 Antigen activates B Cell Receptor (BCR) leading to generation of second messengers
SignaLinkiQ08012

Names & Taxonomyi

Protein namesi
Recommended name:
Protein enhancer of sevenless 2B
Short name:
Protein E(sev)2B
Alternative name(s):
Downstream of receptor kinase
SH2-SH3 adapter protein drk
Gene namesi
Name:drk
Synonyms:E(sev)2B
ORF Names:CG6033
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0004638 drk

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi49P → L: Inactivates the SH3 domain. Abolishes binding to Dab; when associated with R-199. 1 Publication1
Mutagenesisi67R → H in Su(sevs11)R1 mutant; ommatidial cell development obstruction. 1 Publication1
Mutagenesisi85R → K: Disrupts the SH2 domain. Does not affect binding to Dab. 1 Publication1
Mutagenesisi106H → Y in E(sev)2B mutant; ommatidial cell development obstruction. 1 Publication1
Mutagenesisi199G → R: Disrupts the SH3 domain. Abolishes binding to Dab; when associated with L-49. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000882101 – 211Protein enhancer of sevenless 2BAdd BLAST211

Proteomic databases

PaxDbiQ08012
PRIDEiQ08012

Expressioni

Tissue specificityi

Found mainly in the developing eye and in the antennal disk. Also observed in other imaginal disks tested and in the embryo.

Gene expression databases

BgeeiFBgn0004638
GenevisibleiQ08012 DM

Interactioni

Subunit structurei

Interacts with autophosphorylated sev via SH2 domain and Sos, Dos and Dab via SH3 domains. Binds to tyrosine phosphorylated Dab via the SH2 domain.4 Publications

GO - Molecular functioni

  • sevenless binding Source: FlyBase
  • SH3/SH2 adaptor activity Source: FlyBase

Protein-protein interaction databases

BioGridi62256, 75 interactors
DIPiDIP-21223N
IntActiQ08012, 4 interactors
MINTiQ08012
STRINGi7227.FBpp0086812

Structurei

Secondary structure

1211
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 5Combined sources3
Beta strandi13 – 16Combined sources4
Beta strandi23 – 29Combined sources7
Turni31 – 33Combined sources3
Beta strandi36 – 41Combined sources6
Beta strandi44 – 50Combined sources7
Beta strandi53 – 55Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2A36NMR-A1-59[»]
2A37NMR-A1-59[»]
2AZSNMR-A1-59[»]
2AZVNMR-A1-59[»]
ProteinModelPortaliQ08012
SMRiQ08012
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ08012

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 58SH3 1PROSITE-ProRule annotationAdd BLAST58
Domaini60 – 151SH2PROSITE-ProRule annotationAdd BLAST92
Domaini152 – 211SH3 2PROSITE-ProRule annotationAdd BLAST60

Sequence similaritiesi

Belongs to the GRB2/sem-5/DRK family.Curated

Keywords - Domaini

Repeat, SH2 domain, SH3 domain

Phylogenomic databases

eggNOGiKOG3601 Eukaryota
ENOG410XR1G LUCA
GeneTreeiENSGT00820000126999
InParanoidiQ08012
KOiK04364
OMAiHWWHGEI
OrthoDBiEOG091G0HWS
PhylomeDBiQ08012

Family and domain databases

Gene3Di3.30.505.10, 1 hit
InterProiView protein in InterPro
IPR030219 Grb2
IPR000980 SH2
IPR036860 SH2_dom_sf
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PANTHERiPTHR24418:SF290 PTHR24418:SF290, 1 hit
PfamiView protein in Pfam
PF00017 SH2, 1 hit
PF00018 SH3_1, 2 hits
PRINTSiPR00401 SH2DOMAIN
PR00452 SH3DOMAIN
SMARTiView protein in SMART
SM00252 SH2, 1 hit
SM00326 SH3, 2 hits
SUPFAMiSSF50044 SSF50044, 2 hits
SSF55550 SSF55550, 1 hit
PROSITEiView protein in PROSITE
PS50001 SH2, 1 hit
PS50002 SH3, 2 hits

Sequencei

Sequence statusi: Complete.

Q08012-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAIAKHDFS ATADDELSFR KTQILKILNM EDDSNWYRAE LDGKEGLIPS
60 70 80 90 100
NYIEMKNHDW YYGRITRADA EKLLSNKHEG AFLIRISESS PGDFSLSVKC
110 120 130 140 150
PDGVQHFKVL RDAQSKFFLW VVKFNSLNEL VEYHRTASVS RSQDVKLRDM
160 170 180 190 200
IPEEMLVQAL YDFVPQESGE LDFRRGDVIT VTDRSDENWW NGEIGNRKGI
210
FPATYVTPYH S
Length:211
Mass (Da):24,435
Last modified:February 1, 1995 - v1
Checksum:iA1D0614AF358F3C0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12446 mRNA Translation: AAA28898.1
L13173 mRNA No translation available.
AY135053 Genomic DNA Translation: AAN17564.1
AY135054 Genomic DNA Translation: AAN17565.1
AY135055 Genomic DNA Translation: AAN17566.1
AY135056 Genomic DNA Translation: AAN17567.1
AY135057 Genomic DNA Translation: AAN17568.1
AY135058 Genomic DNA Translation: AAN17569.1
AY135059 Genomic DNA Translation: AAN17570.1
AY135060 Genomic DNA Translation: AAN17571.1
AY135061 Genomic DNA Translation: AAN17572.1
AY135062 Genomic DNA Translation: AAN17573.1
AY135063 Genomic DNA Translation: AAN17574.1
AY135064 Genomic DNA Translation: AAN17575.1
AY135065 Genomic DNA Translation: AAN17576.1
AY135066 Genomic DNA Translation: AAN17577.1
AY135067 Genomic DNA Translation: AAN17578.1
AY135068 Genomic DNA Translation: AAN17579.1
AY135069 Genomic DNA Translation: AAN17580.1
AY135070 Genomic DNA Translation: AAN17581.1
AY135071 Genomic DNA Translation: AAN17582.1
AY135072 Genomic DNA Translation: AAN17583.1
AY135073 Genomic DNA Translation: AAN17584.1
AY135074 Genomic DNA Translation: AAN17585.1
AE013599 Genomic DNA Translation: AAF58368.1
AE013599 Genomic DNA Translation: AAM68582.1
AE013599 Genomic DNA Translation: AAM68583.1
AE013599 Genomic DNA Translation: AAM68584.1
AE013599 Genomic DNA Translation: AAM68585.1
AY061142 mRNA Translation: AAL28690.1
PIRiA46444
RefSeqiNP_476858.1, NM_057510.4
NP_725302.1, NM_165994.2
NP_725303.1, NM_165995.2
NP_725304.1, NM_165996.2
NP_725306.1, NM_165998.2
UniGeneiDm.3707

Genome annotation databases

EnsemblMetazoaiFBtr0087694; FBpp0086813; FBgn0004638
FBtr0087695; FBpp0086814; FBgn0004638
FBtr0087696; FBpp0086815; FBgn0004638
FBtr0087697; FBpp0086816; FBgn0004638
FBtr0087698; FBpp0086817; FBgn0004638
GeneIDi36497
KEGGidme:Dmel_CG6033
UCSCiCG6033-RC d. melanogaster

Similar proteinsi

Entry informationi

Entry nameiDRK_DROME
AccessioniPrimary (citable) accession number: Q08012
Secondary accession number(s): A4UZF9, Q0E989, Q9V6Q5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 23, 2018
This is version 171 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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