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Protein

Protein enhancer of sevenless 2B

Gene

drk

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for proper signaling by sevenless. May act to stimulate the ability of Sos to catalyze Ras1 activation by linking sevenless and Sos in a signaling complex.2 Publications

GO - Molecular functioni

  • sevenless binding Source: FlyBase
  • SH3/SH2 adaptor activity Source: UniProtKB
  • signal transducer activity Source: UniProtKB-KW

GO - Biological processi

  • actin filament organization Source: FlyBase
  • activation of JNKK activity Source: UniProtKB
  • anterior/posterior axis specification, embryo Source: FlyBase
  • associative learning Source: FlyBase
  • embryonic development via the syncytial blastoderm Source: FlyBase
  • imaginal disc-derived wing morphogenesis Source: FlyBase
  • negative regulation of epidermal growth factor-activated receptor activity Source: FlyBase
  • olfactory learning Source: FlyBase
  • ovarian follicle cell development Source: FlyBase
  • positive regulation of MAPK cascade Source: FlyBase
  • positive regulation of R7 cell differentiation Source: FlyBase
  • Ras protein signal transduction Source: FlyBase
  • regulation of cell shape Source: FlyBase
  • sevenless signaling pathway Source: FlyBase
  • short-term memory Source: FlyBase
  • TORC1 signaling Source: FlyBase
  • torso signaling pathway Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Enzyme and pathway databases

ReactomeiR-DME-109704. PI3K Cascade.
R-DME-112412. SOS-mediated signalling.
R-DME-1250347. SHC1 events in ERBB4 signaling.
R-DME-1257604. PIP3 activates AKT signaling.
R-DME-1433557. Signaling by SCF-KIT.
R-DME-1433559. Regulation of KIT signaling.
R-DME-167044. Signalling to RAS.
R-DME-179812. GRB2 events in EGFR signaling.
R-DME-180292. GAB1 signalosome.
R-DME-180336. SHC1 events in EGFR signaling.
R-DME-182971. EGFR downregulation.
R-DME-1963640. GRB2 events in ERBB2 signaling.
R-DME-2029482. Regulation of actin dynamics for phagocytic cup formation.
R-DME-210993. Tie2 Signaling.
R-DME-2179392. EGFR Transactivation by Gastrin.
R-DME-2424491. DAP12 signaling.
R-DME-2428933. SHC-related events triggered by IGF1R.
R-DME-2730905. Role of LAT2/NTAL/LAB on calcium mobilization.
R-DME-2871796. FCERI mediated MAPK activation.
R-DME-354194. GRB2:SOS provides linkage to MAPK signaling for Integrins.
R-DME-375165. NCAM signaling for neurite out-growth.
R-DME-5654688. SHC-mediated cascade:FGFR1.
R-DME-5654689. PI-3K cascade:FGFR1.
R-DME-5654693. FRS-mediated FGFR1 signaling.
R-DME-5654695. PI-3K cascade:FGFR2.
R-DME-5654699. SHC-mediated cascade:FGFR2.
R-DME-5654700. FRS-mediated FGFR2 signaling.
R-DME-5654704. SHC-mediated cascade:FGFR3.
R-DME-5654706. FRS-mediated FGFR3 signaling.
R-DME-5654710. PI-3K cascade:FGFR3.
R-DME-5654712. FRS-mediated FGFR4 signaling.
R-DME-5654719. SHC-mediated cascade:FGFR4.
R-DME-5654720. PI-3K cascade:FGFR4.
R-DME-5654726. Negative regulation of FGFR1 signaling.
R-DME-5654727. Negative regulation of FGFR2 signaling.
R-DME-5654732. Negative regulation of FGFR3 signaling.
R-DME-5654733. Negative regulation of FGFR4 signaling.
R-DME-5663213. RHO GTPases Activate WASPs and WAVEs.
R-DME-5673001. RAF/MAP kinase cascade.
R-DME-6807004. Negative regulation of MET activity.
R-DME-6811558. PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling.
R-DME-74749. Signal attenuation.
R-DME-74751. Insulin receptor signalling cascade.
R-DME-8851805. MET activates RAS signaling.
R-DME-8851907. MET activates PI3K/AKT signaling.
R-DME-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-DME-8856828. Clathrin-mediated endocytosis.
R-DME-8865999. MET activates PTPN11.
R-DME-8875555. MET activates RAP1 and RAC1.
R-DME-8875656. MET receptor recycling.
R-DME-912631. Regulation of signaling by CBL.
R-DME-983695. Antigen activates B Cell Receptor (BCR) leading to generation of second messengers.
SignaLinkiQ08012.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein enhancer of sevenless 2B
Short name:
Protein E(sev)2B
Alternative name(s):
Downstream of receptor kinase
SH2-SH3 adapter protein drk
Gene namesi
Name:drk
Synonyms:E(sev)2B
ORF Names:CG6033
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0004638. drk.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: FlyBase
  • nucleoplasm Source: FlyBase
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi49P → L: Inactivates the SH3 domain. Abolishes binding to Dab; when associated with R-199. 1 Publication1
Mutagenesisi67R → H in Su(sevs11)R1 mutant; ommatidial cell development obstruction. 1 Publication1
Mutagenesisi85R → K: Disrupts the SH2 domain. Does not affect binding to Dab. 1 Publication1
Mutagenesisi106H → Y in E(sev)2B mutant; ommatidial cell development obstruction. 1 Publication1
Mutagenesisi199G → R: Disrupts the SH3 domain. Abolishes binding to Dab; when associated with L-49. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000882101 – 211Protein enhancer of sevenless 2BAdd BLAST211

Proteomic databases

PaxDbiQ08012.
PRIDEiQ08012.

Expressioni

Tissue specificityi

Found mainly in the developing eye and in the antennal disk. Also observed in other imaginal disks tested and in the embryo.

Gene expression databases

BgeeiFBgn0004638.
GenevisibleiQ08012. DM.

Interactioni

Subunit structurei

Interacts with autophosphorylated sev via SH2 domain and Sos, Dos and Dab via SH3 domains. Binds to tyrosine phosphorylated Dab via the SH2 domain.4 Publications

GO - Molecular functioni

  • sevenless binding Source: FlyBase
  • SH3/SH2 adaptor activity Source: UniProtKB

Protein-protein interaction databases

BioGridi62256. 27 interactors.
DIPiDIP-21223N.
IntActiQ08012. 4 interactors.
MINTiMINT-242311.
STRINGi7227.FBpp0086812.

Structurei

Secondary structure

1211
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 5Combined sources3
Beta strandi13 – 16Combined sources4
Beta strandi23 – 29Combined sources7
Turni31 – 33Combined sources3
Beta strandi36 – 41Combined sources6
Beta strandi44 – 50Combined sources7
Beta strandi53 – 55Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2A36NMR-A1-59[»]
2A37NMR-A1-59[»]
2AZSNMR-A1-59[»]
2AZVNMR-A1-59[»]
ProteinModelPortaliQ08012.
SMRiQ08012.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ08012.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 58SH3 1PROSITE-ProRule annotationAdd BLAST58
Domaini60 – 151SH2PROSITE-ProRule annotationAdd BLAST92
Domaini152 – 211SH3 2PROSITE-ProRule annotationAdd BLAST60

Sequence similaritiesi

Belongs to the GRB2/sem-5/DRK family.Curated
Contains 1 SH2 domain.PROSITE-ProRule annotation
Contains 2 SH3 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH2 domain, SH3 domain

Phylogenomic databases

eggNOGiKOG3601. Eukaryota.
ENOG410XR1G. LUCA.
GeneTreeiENSGT00820000126999.
InParanoidiQ08012.
KOiK04364.
OMAiYDFTPQE.
OrthoDBiEOG091G0HWS.
PhylomeDBiQ08012.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR000980. SH2.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00017. SH2. 1 hit.
PF00018. SH3_1. 2 hits.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
SMARTiSM00252. SH2. 1 hit.
SM00326. SH3. 2 hits.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 2 hits.
SSF55550. SSF55550. 1 hit.
PROSITEiPS50001. SH2. 1 hit.
PS50002. SH3. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q08012-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAIAKHDFS ATADDELSFR KTQILKILNM EDDSNWYRAE LDGKEGLIPS
60 70 80 90 100
NYIEMKNHDW YYGRITRADA EKLLSNKHEG AFLIRISESS PGDFSLSVKC
110 120 130 140 150
PDGVQHFKVL RDAQSKFFLW VVKFNSLNEL VEYHRTASVS RSQDVKLRDM
160 170 180 190 200
IPEEMLVQAL YDFVPQESGE LDFRRGDVIT VTDRSDENWW NGEIGNRKGI
210
FPATYVTPYH S
Length:211
Mass (Da):24,435
Last modified:February 1, 1995 - v1
Checksum:iA1D0614AF358F3C0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12446 mRNA. Translation: AAA28898.1.
L13173 mRNA. No translation available.
AY135053 Genomic DNA. Translation: AAN17564.1.
AY135054 Genomic DNA. Translation: AAN17565.1.
AY135055 Genomic DNA. Translation: AAN17566.1.
AY135056 Genomic DNA. Translation: AAN17567.1.
AY135057 Genomic DNA. Translation: AAN17568.1.
AY135058 Genomic DNA. Translation: AAN17569.1.
AY135059 Genomic DNA. Translation: AAN17570.1.
AY135060 Genomic DNA. Translation: AAN17571.1.
AY135061 Genomic DNA. Translation: AAN17572.1.
AY135062 Genomic DNA. Translation: AAN17573.1.
AY135063 Genomic DNA. Translation: AAN17574.1.
AY135064 Genomic DNA. Translation: AAN17575.1.
AY135065 Genomic DNA. Translation: AAN17576.1.
AY135066 Genomic DNA. Translation: AAN17577.1.
AY135067 Genomic DNA. Translation: AAN17578.1.
AY135068 Genomic DNA. Translation: AAN17579.1.
AY135069 Genomic DNA. Translation: AAN17580.1.
AY135070 Genomic DNA. Translation: AAN17581.1.
AY135071 Genomic DNA. Translation: AAN17582.1.
AY135072 Genomic DNA. Translation: AAN17583.1.
AY135073 Genomic DNA. Translation: AAN17584.1.
AY135074 Genomic DNA. Translation: AAN17585.1.
AE013599 Genomic DNA. Translation: AAF58368.1.
AE013599 Genomic DNA. Translation: AAM68582.1.
AE013599 Genomic DNA. Translation: AAM68583.1.
AE013599 Genomic DNA. Translation: AAM68584.1.
AE013599 Genomic DNA. Translation: AAM68585.1.
AY061142 mRNA. Translation: AAL28690.1.
PIRiA46444.
RefSeqiNP_476858.1. NM_057510.4.
NP_725302.1. NM_165994.2.
NP_725303.1. NM_165995.2.
NP_725304.1. NM_165996.2.
NP_725306.1. NM_165998.2.
UniGeneiDm.3707.

Genome annotation databases

EnsemblMetazoaiFBtr0087694; FBpp0086813; FBgn0004638.
FBtr0087695; FBpp0086814; FBgn0004638.
FBtr0087696; FBpp0086815; FBgn0004638.
FBtr0087697; FBpp0086816; FBgn0004638.
FBtr0087698; FBpp0086817; FBgn0004638.
GeneIDi36497.
KEGGidme:Dmel_CG6033.
UCSCiCG6033-RC. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12446 mRNA. Translation: AAA28898.1.
L13173 mRNA. No translation available.
AY135053 Genomic DNA. Translation: AAN17564.1.
AY135054 Genomic DNA. Translation: AAN17565.1.
AY135055 Genomic DNA. Translation: AAN17566.1.
AY135056 Genomic DNA. Translation: AAN17567.1.
AY135057 Genomic DNA. Translation: AAN17568.1.
AY135058 Genomic DNA. Translation: AAN17569.1.
AY135059 Genomic DNA. Translation: AAN17570.1.
AY135060 Genomic DNA. Translation: AAN17571.1.
AY135061 Genomic DNA. Translation: AAN17572.1.
AY135062 Genomic DNA. Translation: AAN17573.1.
AY135063 Genomic DNA. Translation: AAN17574.1.
AY135064 Genomic DNA. Translation: AAN17575.1.
AY135065 Genomic DNA. Translation: AAN17576.1.
AY135066 Genomic DNA. Translation: AAN17577.1.
AY135067 Genomic DNA. Translation: AAN17578.1.
AY135068 Genomic DNA. Translation: AAN17579.1.
AY135069 Genomic DNA. Translation: AAN17580.1.
AY135070 Genomic DNA. Translation: AAN17581.1.
AY135071 Genomic DNA. Translation: AAN17582.1.
AY135072 Genomic DNA. Translation: AAN17583.1.
AY135073 Genomic DNA. Translation: AAN17584.1.
AY135074 Genomic DNA. Translation: AAN17585.1.
AE013599 Genomic DNA. Translation: AAF58368.1.
AE013599 Genomic DNA. Translation: AAM68582.1.
AE013599 Genomic DNA. Translation: AAM68583.1.
AE013599 Genomic DNA. Translation: AAM68584.1.
AE013599 Genomic DNA. Translation: AAM68585.1.
AY061142 mRNA. Translation: AAL28690.1.
PIRiA46444.
RefSeqiNP_476858.1. NM_057510.4.
NP_725302.1. NM_165994.2.
NP_725303.1. NM_165995.2.
NP_725304.1. NM_165996.2.
NP_725306.1. NM_165998.2.
UniGeneiDm.3707.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2A36NMR-A1-59[»]
2A37NMR-A1-59[»]
2AZSNMR-A1-59[»]
2AZVNMR-A1-59[»]
ProteinModelPortaliQ08012.
SMRiQ08012.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi62256. 27 interactors.
DIPiDIP-21223N.
IntActiQ08012. 4 interactors.
MINTiMINT-242311.
STRINGi7227.FBpp0086812.

Proteomic databases

PaxDbiQ08012.
PRIDEiQ08012.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0087694; FBpp0086813; FBgn0004638.
FBtr0087695; FBpp0086814; FBgn0004638.
FBtr0087696; FBpp0086815; FBgn0004638.
FBtr0087697; FBpp0086816; FBgn0004638.
FBtr0087698; FBpp0086817; FBgn0004638.
GeneIDi36497.
KEGGidme:Dmel_CG6033.
UCSCiCG6033-RC. d. melanogaster.

Organism-specific databases

CTDi36497.
FlyBaseiFBgn0004638. drk.

Phylogenomic databases

eggNOGiKOG3601. Eukaryota.
ENOG410XR1G. LUCA.
GeneTreeiENSGT00820000126999.
InParanoidiQ08012.
KOiK04364.
OMAiYDFTPQE.
OrthoDBiEOG091G0HWS.
PhylomeDBiQ08012.

Enzyme and pathway databases

ReactomeiR-DME-109704. PI3K Cascade.
R-DME-112412. SOS-mediated signalling.
R-DME-1250347. SHC1 events in ERBB4 signaling.
R-DME-1257604. PIP3 activates AKT signaling.
R-DME-1433557. Signaling by SCF-KIT.
R-DME-1433559. Regulation of KIT signaling.
R-DME-167044. Signalling to RAS.
R-DME-179812. GRB2 events in EGFR signaling.
R-DME-180292. GAB1 signalosome.
R-DME-180336. SHC1 events in EGFR signaling.
R-DME-182971. EGFR downregulation.
R-DME-1963640. GRB2 events in ERBB2 signaling.
R-DME-2029482. Regulation of actin dynamics for phagocytic cup formation.
R-DME-210993. Tie2 Signaling.
R-DME-2179392. EGFR Transactivation by Gastrin.
R-DME-2424491. DAP12 signaling.
R-DME-2428933. SHC-related events triggered by IGF1R.
R-DME-2730905. Role of LAT2/NTAL/LAB on calcium mobilization.
R-DME-2871796. FCERI mediated MAPK activation.
R-DME-354194. GRB2:SOS provides linkage to MAPK signaling for Integrins.
R-DME-375165. NCAM signaling for neurite out-growth.
R-DME-5654688. SHC-mediated cascade:FGFR1.
R-DME-5654689. PI-3K cascade:FGFR1.
R-DME-5654693. FRS-mediated FGFR1 signaling.
R-DME-5654695. PI-3K cascade:FGFR2.
R-DME-5654699. SHC-mediated cascade:FGFR2.
R-DME-5654700. FRS-mediated FGFR2 signaling.
R-DME-5654704. SHC-mediated cascade:FGFR3.
R-DME-5654706. FRS-mediated FGFR3 signaling.
R-DME-5654710. PI-3K cascade:FGFR3.
R-DME-5654712. FRS-mediated FGFR4 signaling.
R-DME-5654719. SHC-mediated cascade:FGFR4.
R-DME-5654720. PI-3K cascade:FGFR4.
R-DME-5654726. Negative regulation of FGFR1 signaling.
R-DME-5654727. Negative regulation of FGFR2 signaling.
R-DME-5654732. Negative regulation of FGFR3 signaling.
R-DME-5654733. Negative regulation of FGFR4 signaling.
R-DME-5663213. RHO GTPases Activate WASPs and WAVEs.
R-DME-5673001. RAF/MAP kinase cascade.
R-DME-6807004. Negative regulation of MET activity.
R-DME-6811558. PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling.
R-DME-74749. Signal attenuation.
R-DME-74751. Insulin receptor signalling cascade.
R-DME-8851805. MET activates RAS signaling.
R-DME-8851907. MET activates PI3K/AKT signaling.
R-DME-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-DME-8856828. Clathrin-mediated endocytosis.
R-DME-8865999. MET activates PTPN11.
R-DME-8875555. MET activates RAP1 and RAC1.
R-DME-8875656. MET receptor recycling.
R-DME-912631. Regulation of signaling by CBL.
R-DME-983695. Antigen activates B Cell Receptor (BCR) leading to generation of second messengers.
SignaLinkiQ08012.

Miscellaneous databases

ChiTaRSidrk. fly.
EvolutionaryTraceiQ08012.
GenomeRNAii36497.
PROiQ08012.

Gene expression databases

BgeeiFBgn0004638.
GenevisibleiQ08012. DM.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR000980. SH2.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00017. SH2. 1 hit.
PF00018. SH3_1. 2 hits.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
SMARTiSM00252. SH2. 1 hit.
SM00326. SH3. 2 hits.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 2 hits.
SSF55550. SSF55550. 1 hit.
PROSITEiPS50001. SH2. 1 hit.
PS50002. SH3. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDRK_DROME
AccessioniPrimary (citable) accession number: Q08012
Secondary accession number(s): A4UZF9, Q0E989, Q9V6Q5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 30, 2016
This is version 157 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.