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Q08010

- VP4_ROTB9

UniProt

Q08010 - VP4_ROTB9

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Protein

Outer capsid protein VP4

Gene
N/A
Organism
Rotavirus A (isolate Cow/Germany/993/1983 G18-P[17]-Ix-Rx-Cx-Mx-Ax-Nx-Tx-Ex-Hx) (RV-A)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Spike-forming protein that mediates virion attachment to the host epithelial cell receptors and plays a major role in cell penetration, determination of host range restriction and virulence. Rotavirus entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. According to the considered strain, VP4 seems to essentially target sialic acid and/or the integrin heterodimer ITGA2/ITGB1 (By similarity).By similarity
Outer capsid protein VP5*: forms the spike "foot" and "body". Acts as a membrane permeabilization protein that mediates release of viral particles from endosomal compartments into the cytoplasm. In integrin-dependent strains, VP5* targets the integrin heterodimer ITGA2/ITGB1 for cell attachment (By similarity).By similarity
VP8* forms the head of the spikes. It is the viral hemagglutinin and an important target of neutralizing antibodies. In sialic acid-dependent strains, VP8* binds to host cell sialic acid, most probably a ganglioside, providing the initial contact (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei229 – 2302CleavageBy similarity
Sitei243 – 2442CleavageBy similarity

GO - Biological processi

  1. permeabilization of host organelle membrane involved in viral entry into host cell Source: UniProtKB-KW
  2. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin

Keywords - Biological processi

Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Viral penetration via permeabilization of host membrane, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Outer capsid protein VP4
Alternative name(s):
Hemagglutinin
Cleaved into the following 2 chains:
OrganismiRotavirus A (isolate Cow/Germany/993/1983 G18-P[17]-Ix-Rx-Cx-Mx-Ax-Nx-Tx-Ex-Hx) (RV-A)
Taxonomic identifieri45408 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiBos taurus (Bovine) [TaxID: 9913]

Subcellular locationi

Chain Outer capsid protein VP4 : Virion By similarity. Host rough endoplasmic reticulum Curated
Note: Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles (By similarity).By similarity
Chain Outer capsid protein VP8* : Virion
Note: Outer capsid protein.By similarity
Chain Outer capsid protein VP5* : Virion
Note: Outer capsid protein.By similarity

GO - Cellular componenti

  1. host cell endoplasmic reticulum Source: UniProtKB-KW
  2. viral outer capsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host endoplasmic reticulum, Outer capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 770770Outer capsid protein VP4PRO_0000041015Add
BLAST
Chaini1 – 229229Outer capsid protein VP8*Sequence AnalysisPRO_0000041016Add
BLAST
Chaini244 – 770527Outer capsid protein VP5*Sequence AnalysisPRO_0000041017Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi32 – 321N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi181 – 1811N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi314 ↔ 376Sequence Analysis
Glycosylationi375 – 3751N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi397 – 3971N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi482 – 4821N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi580 – 5801N-linked (GlcNAc...); by hostSequence Analysis

Post-translational modificationi

Proteolytic cleavage by trypsin results in activation of VP4 functions and greatly increases infectivity. The penetration into the host cell is dependent on trypsin treatment of VP4. It produces two peptides, VP5* and VP8* that remain associated with the virion (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

VP4 is a homotrimer (Potential). VP4 adopts a dimeric appearance above the capsid surface, while forming a trimeric base anchored inside the capsid layer. Only hints of the third molecule are observed above the capsid surface. It probably performs a series of molecular rearrangements during viral entry. Prior to trypsin cleavage, it is flexible. The priming trypsin cleavage triggers its rearrangement into rigid spikes with approximate two-fold symmetry of their protruding parts. After an unknown second triggering event, cleaved VP4 may undergo another rearrangement, in which two VP5* subunits fold back on themselves and join a third subunit to form a tightly associated trimer, shaped like a folded umbrella. VP5* is a homotrimer (Potential). The trimer is coiled-coil stabilized by its C-terminus, however, its N-terminus, known as antigen domain or "body", seems to be flexible allowing it to self-associate either as a dimer or a trimer. The two- to three-fold reorganization and fold-back of VP5* may be linked to membrane penetration, by exposing its hydrophobic region. Interacts with host ITGA2 (via ITAG2 I-domain); this interaction occurs when ITGA2 is part of the integrin heterodimer ITGA2/ITGB1. Interacts with host integrin heterodimer ITGA4/ITGB1 and ITGA4/ITGB7 (By similarity).By similarityCurated

Structurei

3D structure databases

ProteinModelPortaliQ08010.
SMRiQ08010. Positions 249-518.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni244 – 476233Antigen domainBy similarityAdd
BLAST
Regioni304 – 3063DGE motif; interaction with ITGA2/ITGB1 heterodimerBy similarity
Regioni385 – 40521Hydrophobic; possible role in virus entry into host cellSequence AnalysisAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili480 – 51435Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi556 – 60954Ser-richAdd
BLAST

Sequence similaritiesi

Belongs to the rotavirus VP4 family.Curated

Keywords - Domaini

Coiled coil

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR000416. Haemagglutinin_VP4.
[Graphical view]
PfamiPF00426. VP4_haemagglut. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q08010-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASLVYRQLL ANSYTSDLQD TIDDISAQKT ENVTVNPGPF AQTGYALVEW
60 70 80 90 100
THGDITTDET VQQTLDGPYA PSSVIIQPQY WVLMNPETAD VIAEADATNK
110 120 130 140 150
KYACVMLAPN TEEGDKQYTI LGRQITINLG NTDQNRYKFF DLASENGETY
160 170 180 190 200
SKIQELLTPN RLNAFMKDQG RLYVYHGTVP NISTGYYTLD DIANVQTNIK
210 220 230 240 250
CNYYIVPKSQ TQQLEDFLKN GLPPIQESRY IMPVERSVQN IYRAKPNEDI
260 270 280 290 300
VISKTSLWKE MQYNRDIVIR FKFGNTIIKS GGLGYKWSEI SYKPMNYEYT
310 320 330 340 350
YERDGETVVA HTTCSVAGVN DFGYNSGSLP TDFVVSKYEV LKGNSYVYID
360 370 380 390 400
YWDDSQAFKN MVYVRSLSAE FNAINCTGGT YDFQLPVGQW PQMRGGNVTL
410 420 430 440 450
NSDAVTLSTQ YTDFVSLNSL RFRFKPAIGE PFFEITRTRE TRLYGLPASN
460 470 480 490 500
PMGGNEYYET AGRFSLISLV PSNDDYQTPI QNSTTVRQDL EQQISDLREE
510 520 530 540 550
FNQLSSEIAM SQLIDLALLP LDMFSMFSGI KSTIDAVKSV TTSVMKKMKT
560 570 580 590 600
STLAKSVSTI TEELSDAATS VSRASSIRSN ASVWNNLVDT RTQTSVATND
610 620 630 640 650
IATQTSRIAS KLRVKEFATQ TEGGLSFNDI SAAVLKTKID KIETVQPKIL
660 670 680 690 700
PTIITESVDK FIPTRQYRII DKDIAYEISN SGKYFAYRVD TFEEVIFDVE
710 720 730 740 750
KFADLVTDSP VISAIIDFKT LKNLNDNFGI TKEQAYNLLR SDPRVLKDFI
760 770
NQNNPIIRNR IEQLILQCRI
Length:770
Mass (Da):86,943
Last modified:November 1, 1995 - v1
Checksum:i098650A26312EE3B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D16352 mRNA. Translation: BAA03855.1.
PIRiA49283.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D16352 mRNA. Translation: BAA03855.1 .
PIRi A49283.

3D structure databases

ProteinModelPortali Q08010.
SMRi Q08010. Positions 249-518.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.60.120.200. 1 hit.
InterProi IPR013320. ConA-like_dom.
IPR000416. Haemagglutinin_VP4.
[Graphical view ]
Pfami PF00426. VP4_haemagglut. 1 hit.
[Graphical view ]
SUPFAMi SSF49899. SSF49899. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "A unique VP4 gene allele carried by an unusual bovine rotavirus strain, 993/83."
    Isegawa Y., Nakagomi O., Bruessow H., Minamoto N., Nakagomi T., Ueda S.
    Virology 198:366-369(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiVP4_ROTB9
AccessioniPrimary (citable) accession number: Q08010
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 29, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

In group A rotaviruses, VP4 defines the P serotype.
This strain has been shown to be sialic acid-independent in cell culture conditions.By similarity

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3