Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q08003

- RFU1_YEAST

UniProt

Q08003 - RFU1_YEAST

Protein

Regulator of free ubiquitin chains 1

Gene

RFU1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Inhibitor of the DOA4 deubiquitinase involved in the regulation of protein degradation by the proteasome and maintenance of a normal level of free ubiquitin.1 Publication

    GO - Molecular functioni

    1. cysteine-type endopeptidase inhibitor activity Source: UniProtKB-KW
    2. enzyme inhibitor activity Source: SGD
    3. protein binding Source: IntAct

    GO - Biological processi

    1. regulation of ubiquitin homeostasis Source: SGD

    Keywords - Molecular functioni

    Protease inhibitor, Thiol protease inhibitor

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32225-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Regulator of free ubiquitin chains 1
    Gene namesi
    Name:RFU1
    Ordered Locus Names:YLR073C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    CYGDiYLR073c.
    SGDiS000004063. RFU1.

    Subcellular locationi

    Endosome 1 Publication

    GO - Cellular componenti

    1. endosome Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 200200Regulator of free ubiquitin chains 1PRO_0000247248Add
    BLAST

    Proteomic databases

    MaxQBiQ08003.
    PaxDbiQ08003.

    Expressioni

    Gene expression databases

    GenevestigatoriQ08003.

    Interactioni

    Subunit structurei

    Interacts with BRO1 and DOA4.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BRO1P485822EBI-2353109,EBI-3768
    DOA4P325712EBI-2353109,EBI-19840

    Protein-protein interaction databases

    BioGridi31347. 18 interactions.
    IntActiQ08003. 2 interactions.
    MINTiMINT-4494675.
    STRINGi4932.YLR073C.

    Structurei

    3D structure databases

    ProteinModelPortaliQ08003.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi75 – 817Poly-Ser

    Sequence similaritiesi

    Belongs to the RFU1 family.Curated

    Phylogenomic databases

    eggNOGiNOG42161.
    HOGENOMiHOG000154103.
    OMAiINANTTP.
    OrthoDBiEOG76TB3M.

    Sequencei

    Sequence statusi: Complete.

    Q08003-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKSSKQLVQD AKDYRFNPAI PLRIYLKTCI GILEKAQCAF QANDLSLSFI    50
    YYFRYVDLLT NKLSRHPELL RMDASSSSSS SYIHKREYLQ LIKLEVPAVC 100
    KIIESLRTQI DSQYSKLQTS LANNIAKPNI NANTTPVQVE QQPLPKKSFD 150
    EYSFNQSISF FQKISNAQLN TGASSQSQAT ARDEAYRLNY PELPRLTFST 200
    Length:200
    Mass (Da):22,850
    Last modified:November 1, 1996 - v1
    Checksum:i1C93378ED78C41A4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z73245 Genomic DNA. Translation: CAA97630.1.
    AY558532 Genomic DNA. Translation: AAS56858.1.
    BK006945 Genomic DNA. Translation: DAA09390.1.
    PIRiS64905.
    RefSeqiNP_013174.1. NM_001181960.1.

    Genome annotation databases

    EnsemblFungiiYLR073C; YLR073C; YLR073C.
    GeneIDi850762.
    KEGGisce:YLR073C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z73245 Genomic DNA. Translation: CAA97630.1 .
    AY558532 Genomic DNA. Translation: AAS56858.1 .
    BK006945 Genomic DNA. Translation: DAA09390.1 .
    PIRi S64905.
    RefSeqi NP_013174.1. NM_001181960.1.

    3D structure databases

    ProteinModelPortali Q08003.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31347. 18 interactions.
    IntActi Q08003. 2 interactions.
    MINTi MINT-4494675.
    STRINGi 4932.YLR073C.

    Proteomic databases

    MaxQBi Q08003.
    PaxDbi Q08003.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLR073C ; YLR073C ; YLR073C .
    GeneIDi 850762.
    KEGGi sce:YLR073C.

    Organism-specific databases

    CYGDi YLR073c.
    SGDi S000004063. RFU1.

    Phylogenomic databases

    eggNOGi NOG42161.
    HOGENOMi HOG000154103.
    OMAi INANTTP.
    OrthoDBi EOG76TB3M.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32225-MONOMER.

    Miscellaneous databases

    NextBioi 966915.
    PROi Q08003.

    Gene expression databases

    Genevestigatori Q08003.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "An inhibitor of a deubiquitinating enzyme regulates ubiquitin homeostasis."
      Kimura Y., Yashiroda H., Kudo T., Koitabashi S., Murata S., Kakizuka A., Tanaka K.
      Cell 137:549-559(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH BRO1 AND DOA4.

    Entry informationi

    Entry nameiRFU1_YEAST
    AccessioniPrimary (citable) accession number: Q08003
    Secondary accession number(s): D6VY74
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 25, 2006
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 377 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3