Reviewed, UniProtKB/Swiss-Prot Q08003 (RFU1_YEAST)

Names and origin

Protein names

Recommended name:
    Regulator of free ubiquitin chains 1

Gene names

Name:	RFU1
Ordered Locus Names:	YLR073C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	200 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Inhibitor of the DOA4 deubiquitinase involved in the regulation of protein degradation by the proteasome and maintenance of a normal level of free ubiquitin. Ref.5
Subunit structure	Interacts with BRO1 and DOA4. Ref.5
Subcellular location	Endosome.
Miscellaneous	Present with 377 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the RFU1 family.

Ontologies

Keywords
Cellular component	Endosome
Molecular function	Protease inhibitor Thiol protease inhibitor
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of ubiquitin homeostasis Ref.5 Inferred from mutant phenotype. Source: SGD
Cellular component	endosome Ref.3 Inferred from direct assay. Source: SGD
Molecular function	cysteine-type endopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Ref.5 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BRO1	P48582	1	EBI-2353109,EBI-3768
DOA4	P32571	1	EBI-2353109,EBI-19840

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 200	200	Regulator of free ubiquitin chains 1		PRO_0000247248
Regions
	Compositional bias	75 – 81	7	Poly-Ser

Sequences

	Sequence		Length	Mass (Da)
	Q08003-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 1C93378ED78C41A4	FASTA	200	22,850
	10 20 30 40 50 60 MKSSKQLVQD AKDYRFNPAI PLRIYLKTCI GILEKAQCAF QANDLSLSFI YYFRYVDLLT 70 80 90 100 110 120 NKLSRHPELL RMDASSSSSS SYIHKREYLQ LIKLEVPAVC KIIESLRTQI DSQYSKLQTS 130 140 150 160 170 180 LANNIAKPNI NANTTPVQVE QQPLPKKSFD EYSFNQSISF FQKISNAQLN TGASSQSQAT 190 200 ARDEAYRLNY PELPRLTFST

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"An inhibitor of a deubiquitinating enzyme regulates ubiquitin homeostasis." Kimura Y., Yashiroda H., Kudo T., Koitabashi S., Murata S., Kakizuka A., Tanaka K. Cell 137:549-559(2009) [PubMed: 19410548] [Abstract] Cited for: FUNCTION, INTERACTION WITH BRO1 AND DOA4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z73245 Genomic DNA. Translation: CAA97630.1. AY558532 Genomic DNA. Translation: AAS56858.1.
PIR	S64905.
RefSeq	NP_013174.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q08003. 2 interactions.
Genome annotation databases
Ensembl	YLR073C; YLR073C; YLR073C; Saccharomyces cerevisiae. [Genome view]
GeneID	850762.
KEGG	sce:YLR073C.
NMPDR	fig|4932.3.peg.4165.
Organism-specific databases
CYGD	YLR073c.
SGD	S000004063. YLR073C.
Phylogenomic databases
HOGENOM	Q08003.
OMA	CISIVEE
OrthoDB	EOG9HB036
Gene expression databases
ArrayExpress	Q08003.
Genevestigator	Q08003.
GermOnline	YLR073C. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	966915.

Entry information

Entry name

RFU1_YEAST

Accession

Primary (citable) accession number: Q08003

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	November 1, 1996
Last modified:	November 24, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)