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Protein

Regulator of free ubiquitin chains 1

Gene

RFU1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Inhibitor of the DOA4 deubiquitinase involved in the regulation of protein degradation by the proteasome and maintenance of a normal level of free ubiquitin.1 Publication

Miscellaneous

Present with 377 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • cysteine-type endopeptidase inhibitor activity Source: UniProtKB-KW
  • enzyme inhibitor activity Source: SGD

GO - Biological processi

  • ubiquitin recycling Source: SGD

Keywordsi

Molecular functionProtease inhibitor, Thiol protease inhibitor

Enzyme and pathway databases

BioCyciYEAST:G3O-32225-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Regulator of free ubiquitin chains 1
Gene namesi
Name:RFU1
Ordered Locus Names:YLR073C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR073C
SGDiS000004063 RFU1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002472481 – 200Regulator of free ubiquitin chains 1Add BLAST200

Proteomic databases

MaxQBiQ08003
PaxDbiQ08003
PRIDEiQ08003

Interactioni

Subunit structurei

Interacts with BRO1 and DOA4.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi31347, 48 interactors
IntActiQ08003, 4 interactors
MINTiQ08003
STRINGi4932.YLR073C

Structurei

3D structure databases

ProteinModelPortaliQ08003
SMRiQ08003
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi75 – 81Poly-Ser7

Sequence similaritiesi

Belongs to the RFU1 family.Curated

Phylogenomic databases

HOGENOMiHOG000154103
InParanoidiQ08003
OMAiINANTTP
OrthoDBiEOG092C51XK

Sequencei

Sequence statusi: Complete.

Q08003-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSSKQLVQD AKDYRFNPAI PLRIYLKTCI GILEKAQCAF QANDLSLSFI
60 70 80 90 100
YYFRYVDLLT NKLSRHPELL RMDASSSSSS SYIHKREYLQ LIKLEVPAVC
110 120 130 140 150
KIIESLRTQI DSQYSKLQTS LANNIAKPNI NANTTPVQVE QQPLPKKSFD
160 170 180 190 200
EYSFNQSISF FQKISNAQLN TGASSQSQAT ARDEAYRLNY PELPRLTFST
Length:200
Mass (Da):22,850
Last modified:November 1, 1996 - v1
Checksum:i1C93378ED78C41A4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73245 Genomic DNA Translation: CAA97630.1
AY558532 Genomic DNA Translation: AAS56858.1
BK006945 Genomic DNA Translation: DAA09390.1
PIRiS64905
RefSeqiNP_013174.1, NM_001181960.1

Genome annotation databases

EnsemblFungiiYLR073C; YLR073C; YLR073C
GeneIDi850762
KEGGisce:YLR073C

Similar proteinsi

Entry informationi

Entry nameiRFU1_YEAST
AccessioniPrimary (citable) accession number: Q08003
Secondary accession number(s): D6VY74
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health