Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Regulator of free ubiquitin chains 1

Gene

RFU1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Inhibitor of the DOA4 deubiquitinase involved in the regulation of protein degradation by the proteasome and maintenance of a normal level of free ubiquitin.1 Publication

GO - Molecular functioni

  • cysteine-type endopeptidase inhibitor activity Source: UniProtKB-KW
  • enzyme inhibitor activity Source: SGD

GO - Biological processi

  • regulation of ubiquitin homeostasis Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Thiol protease inhibitor

Enzyme and pathway databases

BioCyciYEAST:G3O-32225-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Regulator of free ubiquitin chains 1
Gene namesi
Name:RFU1
Ordered Locus Names:YLR073C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR073C.
SGDiS000004063. RFU1.

Subcellular locationi

GO - Cellular componenti

  • endosome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002472481 – 200Regulator of free ubiquitin chains 1Add BLAST200

Proteomic databases

MaxQBiQ08003.
PRIDEiQ08003.

Interactioni

Subunit structurei

Interacts with BRO1 and DOA4.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
BRO1P485822EBI-2353109,EBI-3768
DOA4P325712EBI-2353109,EBI-19840

Protein-protein interaction databases

BioGridi31347. 20 interactors.
IntActiQ08003. 2 interactors.
MINTiMINT-4494675.

Structurei

3D structure databases

ProteinModelPortaliQ08003.
SMRiQ08003.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi75 – 81Poly-Ser7

Sequence similaritiesi

Belongs to the RFU1 family.Curated

Phylogenomic databases

HOGENOMiHOG000154103.
InParanoidiQ08003.
OMAiINANTTP.
OrthoDBiEOG092C51XK.

Sequencei

Sequence statusi: Complete.

Q08003-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSSKQLVQD AKDYRFNPAI PLRIYLKTCI GILEKAQCAF QANDLSLSFI
60 70 80 90 100
YYFRYVDLLT NKLSRHPELL RMDASSSSSS SYIHKREYLQ LIKLEVPAVC
110 120 130 140 150
KIIESLRTQI DSQYSKLQTS LANNIAKPNI NANTTPVQVE QQPLPKKSFD
160 170 180 190 200
EYSFNQSISF FQKISNAQLN TGASSQSQAT ARDEAYRLNY PELPRLTFST
Length:200
Mass (Da):22,850
Last modified:November 1, 1996 - v1
Checksum:i1C93378ED78C41A4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73245 Genomic DNA. Translation: CAA97630.1.
AY558532 Genomic DNA. Translation: AAS56858.1.
BK006945 Genomic DNA. Translation: DAA09390.1.
PIRiS64905.
RefSeqiNP_013174.1. NM_001181960.1.

Genome annotation databases

EnsemblFungiiYLR073C; YLR073C; YLR073C.
GeneIDi850762.
KEGGisce:YLR073C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73245 Genomic DNA. Translation: CAA97630.1.
AY558532 Genomic DNA. Translation: AAS56858.1.
BK006945 Genomic DNA. Translation: DAA09390.1.
PIRiS64905.
RefSeqiNP_013174.1. NM_001181960.1.

3D structure databases

ProteinModelPortaliQ08003.
SMRiQ08003.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31347. 20 interactors.
IntActiQ08003. 2 interactors.
MINTiMINT-4494675.

Proteomic databases

MaxQBiQ08003.
PRIDEiQ08003.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR073C; YLR073C; YLR073C.
GeneIDi850762.
KEGGisce:YLR073C.

Organism-specific databases

EuPathDBiFungiDB:YLR073C.
SGDiS000004063. RFU1.

Phylogenomic databases

HOGENOMiHOG000154103.
InParanoidiQ08003.
OMAiINANTTP.
OrthoDBiEOG092C51XK.

Enzyme and pathway databases

BioCyciYEAST:G3O-32225-MONOMER.

Miscellaneous databases

PROiQ08003.

Family and domain databases

ProtoNetiSearch...

Entry informationi

Entry nameiRFU1_YEAST
AccessioniPrimary (citable) accession number: Q08003
Secondary accession number(s): D6VY74
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 377 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.