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Q08003

- RFU1_YEAST

UniProt

Q08003 - RFU1_YEAST

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Protein
Regulator of free ubiquitin chains 1
Gene
RFU1, YLR073C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Inhibitor of the DOA4 deubiquitinase involved in the regulation of protein degradation by the proteasome and maintenance of a normal level of free ubiquitin.1 Publication

GO - Molecular functioni

  1. cysteine-type endopeptidase inhibitor activity Source: UniProtKB-KW
  2. enzyme inhibitor activity Source: SGD
  3. protein binding Source: IntAct

GO - Biological processi

  1. regulation of ubiquitin homeostasis Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Thiol protease inhibitor

Enzyme and pathway databases

BioCyciYEAST:G3O-32225-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Regulator of free ubiquitin chains 1
Gene namesi
Name:RFU1
Ordered Locus Names:YLR073C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XII

Organism-specific databases

CYGDiYLR073c.
SGDiS000004063. RFU1.

Subcellular locationi

Endosome 1 Publication

GO - Cellular componenti

  1. endosome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 200200Regulator of free ubiquitin chains 1
PRO_0000247248Add
BLAST

Proteomic databases

MaxQBiQ08003.
PaxDbiQ08003.

Expressioni

Gene expression databases

GenevestigatoriQ08003.

Interactioni

Subunit structurei

Interacts with BRO1 and DOA4.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
BRO1P485822EBI-2353109,EBI-3768
DOA4P325712EBI-2353109,EBI-19840

Protein-protein interaction databases

BioGridi31347. 18 interactions.
IntActiQ08003. 2 interactions.
MINTiMINT-4494675.
STRINGi4932.YLR073C.

Structurei

3D structure databases

ProteinModelPortaliQ08003.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi75 – 817Poly-Ser

Sequence similaritiesi

Belongs to the RFU1 family.

Phylogenomic databases

eggNOGiNOG42161.
HOGENOMiHOG000154103.
OMAiINANTTP.
OrthoDBiEOG76TB3M.

Sequencei

Sequence statusi: Complete.

Q08003-1 [UniParc]FASTAAdd to Basket

« Hide

MKSSKQLVQD AKDYRFNPAI PLRIYLKTCI GILEKAQCAF QANDLSLSFI    50
YYFRYVDLLT NKLSRHPELL RMDASSSSSS SYIHKREYLQ LIKLEVPAVC 100
KIIESLRTQI DSQYSKLQTS LANNIAKPNI NANTTPVQVE QQPLPKKSFD 150
EYSFNQSISF FQKISNAQLN TGASSQSQAT ARDEAYRLNY PELPRLTFST 200
Length:200
Mass (Da):22,850
Last modified:November 1, 1996 - v1
Checksum:i1C93378ED78C41A4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z73245 Genomic DNA. Translation: CAA97630.1.
AY558532 Genomic DNA. Translation: AAS56858.1.
BK006945 Genomic DNA. Translation: DAA09390.1.
PIRiS64905.
RefSeqiNP_013174.1. NM_001181960.1.

Genome annotation databases

EnsemblFungiiYLR073C; YLR073C; YLR073C.
GeneIDi850762.
KEGGisce:YLR073C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z73245 Genomic DNA. Translation: CAA97630.1 .
AY558532 Genomic DNA. Translation: AAS56858.1 .
BK006945 Genomic DNA. Translation: DAA09390.1 .
PIRi S64905.
RefSeqi NP_013174.1. NM_001181960.1.

3D structure databases

ProteinModelPortali Q08003.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31347. 18 interactions.
IntActi Q08003. 2 interactions.
MINTi MINT-4494675.
STRINGi 4932.YLR073C.

Proteomic databases

MaxQBi Q08003.
PaxDbi Q08003.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YLR073C ; YLR073C ; YLR073C .
GeneIDi 850762.
KEGGi sce:YLR073C.

Organism-specific databases

CYGDi YLR073c.
SGDi S000004063. RFU1.

Phylogenomic databases

eggNOGi NOG42161.
HOGENOMi HOG000154103.
OMAi INANTTP.
OrthoDBi EOG76TB3M.

Enzyme and pathway databases

BioCyci YEAST:G3O-32225-MONOMER.

Miscellaneous databases

NextBioi 966915.
PROi Q08003.

Gene expression databases

Genevestigatori Q08003.

Family and domain databases

ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "An inhibitor of a deubiquitinating enzyme regulates ubiquitin homeostasis."
    Kimura Y., Yashiroda H., Kudo T., Koitabashi S., Murata S., Kakizuka A., Tanaka K.
    Cell 137:549-559(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH BRO1 AND DOA4.

Entry informationi

Entry nameiRFU1_YEAST
AccessioniPrimary (citable) accession number: Q08003
Secondary accession number(s): D6VY74
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: November 1, 1996
Last modified: September 3, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 377 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

External Data

Dasty 3

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