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Q08003 (RFU1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Regulator of free ubiquitin chains 1
Gene names
Name:RFU1
Ordered Locus Names:YLR073C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length200 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inhibitor of the DOA4 deubiquitinase involved in the regulation of protein degradation by the proteasome and maintenance of a normal level of free ubiquitin. Ref.6

Subunit structure

Interacts with BRO1 and DOA4. Ref.6

Subcellular location

Endosome Ref.4.

Miscellaneous

Present with 377 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the RFU1 family.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 200200Regulator of free ubiquitin chains 1
PRO_0000247248

Regions

Compositional bias75 – 817Poly-Ser

Sequences

Sequence LengthMass (Da)Tools
Q08003 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 1C93378ED78C41A4

FASTA20022,850
        10         20         30         40         50         60 
MKSSKQLVQD AKDYRFNPAI PLRIYLKTCI GILEKAQCAF QANDLSLSFI YYFRYVDLLT 

        70         80         90        100        110        120 
NKLSRHPELL RMDASSSSSS SYIHKREYLQ LIKLEVPAVC KIIESLRTQI DSQYSKLQTS 

       130        140        150        160        170        180 
LANNIAKPNI NANTTPVQVE QQPLPKKSFD EYSFNQSISF FQKISNAQLN TGASSQSQAT 

       190        200 
ARDEAYRLNY PELPRLTFST 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. expand/collapse author list , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"An inhibitor of a deubiquitinating enzyme regulates ubiquitin homeostasis."
Kimura Y., Yashiroda H., Kudo T., Koitabashi S., Murata S., Kakizuka A., Tanaka K.
Cell 137:549-559(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH BRO1 AND DOA4.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z73245 Genomic DNA. Translation: CAA97630.1.
AY558532 Genomic DNA. Translation: AAS56858.1.
BK006945 Genomic DNA. Translation: DAA09390.1.
PIRS64905.
RefSeqNP_013174.1. NM_001181960.1.

3D structure databases

ProteinModelPortalQ08003.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31347. 18 interactions.
IntActQ08003. 2 interactions.
MINTMINT-4494675.
STRING4932.YLR073C.

Proteomic databases

MaxQBQ08003.
PaxDbQ08003.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYLR073C; YLR073C; YLR073C.
GeneID850762.
KEGGsce:YLR073C.

Organism-specific databases

CYGDYLR073c.
SGDS000004063. YLR073C.

Phylogenomic databases

eggNOGNOG42161.
HOGENOMHOG000154103.
OMAINANTTP.
OrthoDBEOG76TB3M.

Enzyme and pathway databases

BioCycYEAST:G3O-32225-MONOMER.

Gene expression databases

GenevestigatorQ08003.

Family and domain databases

ProtoNetSearch...

Other

NextBio966915.
PROQ08003.

Entry information

Entry nameRFU1_YEAST
AccessionPrimary (citable) accession number: Q08003
Secondary accession number(s): D6VY74
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families