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Protein

Thymidylate synthase

Gene

thyA

Organism
Shewanella frigidimarina (strain NCIMB 400)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei22dUMPUniRule annotation1
Active sitei160NucleophileUniRule annotation1
Binding sitei1835,10-methylenetetrahydrofolateUniRule annotation1
Binding sitei191dUMPUniRule annotation1
Binding sitei2825,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi180 – 183dUMPUniRule annotation4
Nucleotide bindingi221 – 223dUMPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processNucleotide biosynthesis

Enzyme and pathway databases

BioCyciSFRI318167:G1G77-3061-MONOMER
UniPathwayiUPA00575

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
Ordered Locus Names:Sfri_2942
OrganismiShewanella frigidimarina (strain NCIMB 400)
Taxonomic identifieri318167 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
Proteomesi
  • UP000000684 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000006711 – 283Thymidylate synthaseAdd BLAST283

Proteomic databases

PRIDEiQ07YY3

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi318167.Sfri_2942

Structurei

3D structure databases

ProteinModelPortaliQ07YY3
SMRiQ07YY3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0V Bacteria
COG0207 LUCA
HOGENOMiHOG000257900
KOiK00560
OMAiKQYLDLC
OrthoDBiPOG091H02L6

Family and domain databases

CDDicd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Di3.30.572.10, 1 hit
HAMAPiMF_00008 Thymidy_synth_bact, 1 hit
InterProiView protein in InterPro
IPR023451 Thymidate_synth/dCMP_Mease
IPR036926 Thymidate_synth/dCMP_Mease_sf
IPR000398 Thymidylate_synthase
IPR020940 Thymidylate_synthase_AS
PfamiView protein in Pfam
PF00303 Thymidylat_synt, 1 hit
PRINTSiPR00108 THYMDSNTHASE
SUPFAMiSSF55831 SSF55831, 1 hit
TIGRFAMsiTIGR03284 thym_sym, 1 hit
PROSITEiView protein in PROSITE
PS00091 THYMIDYLATE_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q07YY3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQYLDLCKR IVDEGEWVNN ARTNKKCLTV INADLVYDVG NNQFPLVTTR
60 70 80 90 100
KSFWKSAIAE ILGYLRGYQN AADFRALGTK TWDANANENQ AWLDNPNRKG
110 120 130 140 150
PDDMGLIYGA LGRAFPKPDG GHVDLLQQIV DDLSNGIDNR GEILTFYHPG
160 170 180 190 200
AFDLGCLRPC MYEHHFSLLG DTLYLNSTQR SCDVPLGLNF NMVQVYVLLA
210 220 230 240 250
LMAQITGHKP GKAYHKIVNA HIYEDQLEPM RDVQLAREPF ASPKLIINPK
260 270 280
IKTLKDIETW VTLDDFSVED YQHHDAIKYP FAV
Length:283
Mass (Da):32,119
Last modified:October 31, 2006 - v1
Checksum:iDCB96B1AF7BB8D4B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000447 Genomic DNA Translation: ABI72781.1
RefSeqiWP_011638390.1, NC_008345.1

Genome annotation databases

EnsemblBacteriaiABI72781; ABI72781; Sfri_2942
KEGGisfr:Sfri_2942

Similar proteinsi

Entry informationi

Entry nameiTYSY_SHEFN
AccessioniPrimary (citable) accession number: Q07YY3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 31, 2006
Last modified: May 23, 2018
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health