Reviewed, UniProtKB/Swiss-Prot Q07YF5 (KATG2_SHEFN)

Names and origin

Protein names

Recommended name:
    Catalase-peroxidase 2
      Short name=CP 2
    EC=1.11.1.6
    EC=1.11.1.7
Alternative name(s):
    Peroxidase/catalase 2

Gene names

Name:	katG2
Ordered Locus Names:	Sfri_3122

Organism

Shewanella frigidimarina (strain NCIMB 400) [Complete proteome] [HAMAP]

Taxonomic identifier

318167 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	757 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity.
Catalytic activity	2 H2O2 = O2 + 2 H2O. HAMAP MF_01961 Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity.
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity.
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Domain	Signal
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: HAMAP heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 757	731	Catalase-peroxidase 2 HAMAP MF_01961		PRO_5000130998
Sites
	Active site	127	1	Proton acceptor By similarity
	Metal binding	289	1	Iron (heme axial ligand) By similarity
	Site	123	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	126 ↔ 248		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-274) By similarity
	Cross-link	248 ↔ 274		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-126) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q07YF5-1 [UniParc]. Last modified October 31, 2006. Version 1. Checksum: B32EA3AA64FB2459	FASTA	757	83,632
	10 20 30 40 50 60 MKHPLFNQKV LAGFVSMLLI SGSAFASNNE KSEMTKPKGA VGTGVALENQ ARTNQFWWPD 70 80 90 100 110 120 QLNLSALRDH DKRSNPYGEN FDYAKAFNSL DLDKVKLDIN ALLTTSQDWW PSDYSNYGPF 130 140 150 160 170 180 FIRMTWHSAG TYRTLDGRGG AGGGQQRFEP LNSWPDNASL DKARRLLWPI KMKYGEAISW 190 200 210 220 230 240 SDLIVLAGNV SLENMGFKTY GFAGGRHDDW EPDMVYWGPE IEMLASDRED NGGKLQRPLG 250 260 270 280 290 300 ATHMGLIYVN PEGPKGVPDP LGSAKNIRVA FERMAMNDEE TLALIAGGHT FGKMHGAHKP 310 320 330 340 350 360 KDCLGAEPAA AGIEEQGLGW KNKCGKGHSE DTITSGLEGA WTQAPTKWTS LYLSNLLTFE 370 380 390 400 410 420 WKQTRSPAGA IQWIPTDESL HKAVPDAHVK GKFHAPVMTT ADLALKYDPE YRKIAERFLA 430 440 450 460 470 480 DPEEYRLAFA KAWYKLTHRD MGPSRNFLGK EVPKESLIWQ DPIDDKTQSN IDADGVQELK 490 500 510 520 530 540 AQILKSNLTV SELVRVAWAS AASYRHSDMR GGANGARIAL SPQKDWSVNN PAETAKVIKT 550 560 570 580 590 600 LKAIQEDYND SLFSKSKVSL ADLIVLGGTA AIEKAAKDAG FTVSVPFNAG RGDATQAMTD 610 620 630 640 650 660 INAFSLLELT SDGFRNYFDA QQSYKSPVDM LVDKADQLNL SVPEMTVLVG GLRALDANYK 670 680 690 700 710 720 GLKHGVLTST PGTLNNDFFV NLLDMSTVWK KSSTDGIYEG FDRQSGHKKW TATSVDLVFG 730 740 750 SNSELRAVSE VYAFDTSKQK FVEDFAAAWT KVMNLDR

References

[1]

"Complete sequence of Shewanella frigidimarina NCIMB 400." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Fredrickson J.K., Kolker E., McCuel L.A., DiChristina T., Nealson K.H., Newman D., Tiedje J.M. , Zhou J., Romine M.F., Culley D.E., Serres M., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000447 Genomic DNA. Translation: ABI72959.1.
RefSeq	YP_751798.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q07YF5.
Genome annotation databases
GeneID	4280025.
GenomeReviews	Gene locus Sfri_3122 in contig CP000447_GR.
KEGG	sfr:Sfri_3122.
NMPDR	fig|318167.10.peg.2998.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q07YF5.
OMA	GTDMRGG.
Family and domain databases
HAMAP	MF_01961. [Tree]
InterPro	IPR000763. Catalase_proxase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
TIGRFAMs	TIGR00198. cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG2_SHEFN

Accession

Primary (citable) accession number: Q07YF5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	October 31, 2006
Last modified:	November 3, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)