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Q07Y78 (HLDE_SHEFN) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bifunctional protein HldE

Including the following 2 domains:

  1. D-beta-D-heptose 7-phosphate kinase
    EC=2.7.1.167
    Alternative name(s):
    D-beta-D-heptose 7-phosphotransferase
    D-glycero-beta-D-manno-heptose-7-phosphate kinase
  2. D-beta-D-heptose 1-phosphate adenylyltransferase
    EC=2.7.7.70
    Alternative name(s):
    D-glycero-beta-D-manno-heptose 1-phosphate adenylyltransferase
Gene names
Name:hldE
Ordered Locus Names:Sfri_3200
OrganismShewanella frigidimarina (strain NCIMB 400) [Complete proteome] [HAMAP]
Taxonomic identifier318167 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella

Protein attributes

Sequence length476 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the phosphorylation of D-glycero-D-manno-heptose 7-phosphate at the C-1 position to selectively form D-glycero-beta-D-manno-heptose-1,7-bisphosphate By similarity. HAMAP-Rule MF_01603

Catalyzes the ADP transfer from ATP to D-glycero-beta-D-manno-heptose 1-phosphate, yielding ADP-D-glycero-beta-D-manno-heptose By similarity. HAMAP-Rule MF_01603

Catalytic activity

D-glycero-beta-D-manno-heptose 7-phosphate + ATP = D-glycero-beta-D-manno-heptose 1,7-bisphosphate + ADP. HAMAP-Rule MF_01603

D-glycero-beta-D-manno-heptose 1-phosphate + ATP = ADP-D-glycero-beta-D-manno-heptose + diphosphate. HAMAP-Rule MF_01603

Pathway

Nucleotide-sugar biosynthesis; ADP-L-glycero-beta-D-manno-heptose biosynthesis; ADP-L-glycero-beta-D-manno-heptose from D-glycero-beta-D-manno-heptose 7-phosphate: step 1/4. HAMAP-Rule MF_01603

Nucleotide-sugar biosynthesis; ADP-L-glycero-beta-D-manno-heptose biosynthesis; ADP-L-glycero-beta-D-manno-heptose from D-glycero-beta-D-manno-heptose 7-phosphate: step 3/4.

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01603

Sequence similarities

In the N-terminal section; belongs to the carbohydrate kinase PfkB family.

In the C-terminal section; belongs to the cytidylyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 476476Bifunctional protein HldE HAMAP-Rule MF_01603
PRO_0000291688

Regions

Nucleotide binding195 – 1984ATP Potential
Region1 – 319319Ribokinase HAMAP-Rule MF_01603
Region345 – 476132Cytidylyltransferase HAMAP-Rule MF_01603

Sites

Active site2641 Potential

Sequences

Sequence LengthMass (Da)Tools
Q07Y78 [UniParc].

Last modified October 31, 2006. Version 1.
Checksum: CFDA4E7393AA3515

FASTA47650,701
        10         20         30         40         50         60 
MKVSLPAFEN ARVLVIGDVM LDRYWVGPTG RISPEAPVPV VKINQIEDRP GGAANVALNI 

        70         80         90        100        110        120 
ATLGGHVQLA GIVGQDETAQ ALTQGVKVFG VEPQWLTVAD KPTITKLRVL SRNQQLIRLD 

       130        140        150        160        170        180 
FEEQFDKATS QALFAQSEAI LDNVDVLVLS DYAKGAIDEP KDFIAKARAK GVKVLVDPKG 

       190        200        210        220        230        240 
HDFARYHGAS LITPNMSEFE AVVGTVTSED DLIEKAQKLI KLHDFEAILV TRSEKGMTLV 

       250        260        270        280        290        300 
SQNQPELHIP TVAREVHDVT GAGDTVISAL ATSIAAGASL AQACAIANTA AGVVVGKLGT 

       310        320        330        340        350        360 
STVSRIELIQ ALALNHGESG FGVMTEDQLA YAMDQARLRG ERIVMTNGCF DILHAGHVSY 

       370        380        390        400        410        420 
LQQAKALGDR LIVAVNDDSS VTRLKGPGRP VNPVDRRMAV LAGLASVDWV VPFTEDTPQR 

       430        440        450        460        470 
IITRLLPDSL VKGGDYKVED IAGGAEVIAA GGKVEVLGFE DGVSTTAIIQ NIMSQK 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000447 Genomic DNA. Translation: ABI73036.1.
RefSeqYP_751875.1. NC_008345.1.

3D structure databases

ProteinModelPortalQ07Y78.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING318167.Sfri_3200.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABI73036; ABI73036; Sfri_3200.
GeneID4280102.
KEGGsfr:Sfri_3200.
PATRIC23500551. VBISheFri14343_3316.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2870.
HOGENOMHOG000237584.
KOK03272.
OMATKLRVMS.
OrthoDBEOG68Q0W4.

Enzyme and pathway databases

BioCycSFRI318167:GIXS-3314-MONOMER.
UniPathwayUPA00356; UER00437.
UPA00356; UER00439.

Family and domain databases

Gene3D3.40.1190.20. 1 hit.
3.40.50.620. 1 hit.
HAMAPMF_01603. HldE.
InterProIPR023030. Bifunc_HldE.
IPR002173. Carboh/pur_kinase_PfkB_CS.
IPR004821. Cyt_trans-like.
IPR011611. PfkB_dom.
IPR011913. RfaE_dom_I.
IPR011914. RfaE_dom_II.
IPR029056. Ribokinase-like.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF01467. CTP_transf_2. 1 hit.
PF00294. PfkB. 1 hit.
[Graphical view]
SUPFAMSSF53613. SSF53613. 1 hit.
TIGRFAMsTIGR00125. cyt_tran_rel. 1 hit.
TIGR02198. rfaE_dom_I. 1 hit.
TIGR02199. rfaE_dom_II. 1 hit.
PROSITEPS00583. PFKB_KINASES_1. 1 hit.
PS00584. PFKB_KINASES_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHLDE_SHEFN
AccessionPrimary (citable) accession number: Q07Y78
Entry history
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: October 31, 2006
Last modified: June 11, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways