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Protein

Bifunctional protein HldE

Gene

hldE

Organism
Shewanella frigidimarina (strain NCIMB 400)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-glycero-D-manno-heptose 7-phosphate at the C-1 position to selectively form D-glycero-beta-D-manno-heptose-1,7-bisphosphate.UniRule annotation
Catalyzes the ADP transfer from ATP to D-glycero-beta-D-manno-heptose 1-phosphate, yielding ADP-D-glycero-beta-D-manno-heptose.UniRule annotation

Catalytic activityi

D-glycero-beta-D-manno-heptose 7-phosphate + ATP = D-glycero-beta-D-manno-heptose 1,7-bisphosphate + ADP.UniRule annotation
D-glycero-beta-D-manno-heptose 1-phosphate + ATP = ADP-D-glycero-beta-D-manno-heptose + diphosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei264 – 2641UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi195 – 1984ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. heptose-1-phosphate adenylyltransferase activity Source: UniProtKB-HAMAP
  3. heptose 7-phosphate kinase activity Source: UniProtKB-HAMAP
  4. phosphotransferase activity, alcohol group as acceptor Source: InterPro

GO - Biological processi

  1. ADP-L-glycero-beta-D-manno-heptose biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSFRI318167:GIXS-3314-MONOMER.
UniPathwayiUPA00356; UER00437.
UPA00356; UER00439.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein HldEUniRule annotation
Including the following 2 domains:
D-beta-D-heptose 7-phosphate kinaseUniRule annotation (EC:2.7.1.167UniRule annotation)
Alternative name(s):
D-beta-D-heptose 7-phosphotransferaseUniRule annotation
D-glycero-beta-D-manno-heptose-7-phosphate kinaseUniRule annotation
D-beta-D-heptose 1-phosphate adenylyltransferaseUniRule annotation (EC:2.7.7.70UniRule annotation)
Alternative name(s):
D-glycero-beta-D-manno-heptose 1-phosphate adenylyltransferaseUniRule annotation
Gene namesi
Name:hldEUniRule annotation
Ordered Locus Names:Sfri_3200
OrganismiShewanella frigidimarina (strain NCIMB 400)
Taxonomic identifieri318167 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
ProteomesiUP000000684 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 476476Bifunctional protein HldEPRO_0000291688Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi318167.Sfri_3200.

Structurei

3D structure databases

ProteinModelPortaliQ07Y78.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 319319RibokinaseAdd
BLAST
Regioni345 – 476132CytidylyltransferaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the carbohydrate kinase PfkB family.UniRule annotation
In the C-terminal section; belongs to the cytidylyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2870.
HOGENOMiHOG000237584.
KOiK03272.
OMAiTKLRVMS.
OrthoDBiEOG68Q0W4.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_01603. HldE.
InterProiIPR023030. Bifunc_HldE.
IPR002173. Carboh/pur_kinase_PfkB_CS.
IPR004821. Cyt_trans-like.
IPR011611. PfkB_dom.
IPR011913. RfaE_dom_I.
IPR011914. RfaE_dom_II.
IPR029056. Ribokinase-like.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01467. CTP_transf_2. 1 hit.
PF00294. PfkB. 1 hit.
[Graphical view]
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00125. cyt_tran_rel. 1 hit.
TIGR02198. rfaE_dom_I. 1 hit.
TIGR02199. rfaE_dom_II. 1 hit.
PROSITEiPS00583. PFKB_KINASES_1. 1 hit.
PS00584. PFKB_KINASES_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q07Y78-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVSLPAFEN ARVLVIGDVM LDRYWVGPTG RISPEAPVPV VKINQIEDRP
60 70 80 90 100
GGAANVALNI ATLGGHVQLA GIVGQDETAQ ALTQGVKVFG VEPQWLTVAD
110 120 130 140 150
KPTITKLRVL SRNQQLIRLD FEEQFDKATS QALFAQSEAI LDNVDVLVLS
160 170 180 190 200
DYAKGAIDEP KDFIAKARAK GVKVLVDPKG HDFARYHGAS LITPNMSEFE
210 220 230 240 250
AVVGTVTSED DLIEKAQKLI KLHDFEAILV TRSEKGMTLV SQNQPELHIP
260 270 280 290 300
TVAREVHDVT GAGDTVISAL ATSIAAGASL AQACAIANTA AGVVVGKLGT
310 320 330 340 350
STVSRIELIQ ALALNHGESG FGVMTEDQLA YAMDQARLRG ERIVMTNGCF
360 370 380 390 400
DILHAGHVSY LQQAKALGDR LIVAVNDDSS VTRLKGPGRP VNPVDRRMAV
410 420 430 440 450
LAGLASVDWV VPFTEDTPQR IITRLLPDSL VKGGDYKVED IAGGAEVIAA
460 470
GGKVEVLGFE DGVSTTAIIQ NIMSQK
Length:476
Mass (Da):50,701
Last modified:October 31, 2006 - v1
Checksum:iCFDA4E7393AA3515
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000447 Genomic DNA. Translation: ABI73036.1.
RefSeqiWP_011638639.1. NC_008345.1.
YP_751875.1. NC_008345.1.

Genome annotation databases

EnsemblBacteriaiABI73036; ABI73036; Sfri_3200.
KEGGisfr:Sfri_3200.
PATRICi23500551. VBISheFri14343_3316.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000447 Genomic DNA. Translation: ABI73036.1.
RefSeqiWP_011638639.1. NC_008345.1.
YP_751875.1. NC_008345.1.

3D structure databases

ProteinModelPortaliQ07Y78.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi318167.Sfri_3200.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABI73036; ABI73036; Sfri_3200.
KEGGisfr:Sfri_3200.
PATRICi23500551. VBISheFri14343_3316.

Phylogenomic databases

eggNOGiCOG2870.
HOGENOMiHOG000237584.
KOiK03272.
OMAiTKLRVMS.
OrthoDBiEOG68Q0W4.

Enzyme and pathway databases

UniPathwayiUPA00356; UER00437.
UPA00356; UER00439.
BioCyciSFRI318167:GIXS-3314-MONOMER.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_01603. HldE.
InterProiIPR023030. Bifunc_HldE.
IPR002173. Carboh/pur_kinase_PfkB_CS.
IPR004821. Cyt_trans-like.
IPR011611. PfkB_dom.
IPR011913. RfaE_dom_I.
IPR011914. RfaE_dom_II.
IPR029056. Ribokinase-like.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01467. CTP_transf_2. 1 hit.
PF00294. PfkB. 1 hit.
[Graphical view]
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00125. cyt_tran_rel. 1 hit.
TIGR02198. rfaE_dom_I. 1 hit.
TIGR02199. rfaE_dom_II. 1 hit.
PROSITEiPS00583. PFKB_KINASES_1. 1 hit.
PS00584. PFKB_KINASES_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NCIMB 400.

Entry informationi

Entry nameiHLDE_SHEFN
AccessioniPrimary (citable) accession number: Q07Y78
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: October 31, 2006
Last modified: April 1, 2015
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.