Q07Y78 (HLDE_SHEFN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bifunctional protein HldE

Including the following 2 domains:

1. D-beta-D-heptose 7-phosphate kinase
   EC=2.7.1.-
   Alternative name(s):
   D-beta-D-heptose 7-phosphotransferase
2. D-beta-D-heptose 1-phosphate adenosyltransferase
   EC=2.7.7.-

Gene names

Name:	hldE
Ordered Locus Names:	Sfri_3200

Organism

Shewanella frigidimarina (strain NCIMB 400) [Complete proteome] [HAMAP]

Taxonomic identifier

318167 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella › Shewanella frigidimarina

Protein attributes

Sequence length	476 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of D-glycero-D-manno-heptose 7-phosphate at the C-1 position to form D,D-heptose-1,7-bisphosphate By similarity. HAMAP-Rule MF_01603 Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose By similarity. HAMAP-Rule MF_01603
Catalytic activity	ATP + D-glycero-beta-D-manno-heptose 7-phosphate = ADP + D-glycero-beta-D-manno-heptose 1,7-bisphosphate. HAMAP-Rule MF_01603 ATP + D-glycero-beta-D-manno-heptose 1-phosphate = diphosphate + ADP-D-glycero-beta-D-manno-heptose. HAMAP-Rule MF_01603
Pathway	Nucleotide-sugar biosynthesis; ADP-L-glycero-beta-D-manno-heptose biosynthesis; ADP-L-glycero-beta-D-manno-heptose from D-glycero-beta-D-manno-heptose 7-phosphate: step 1/4. HAMAP-Rule MF_01603 Nucleotide-sugar biosynthesis; ADP-L-glycero-beta-D-manno-heptose biosynthesis; ADP-L-glycero-beta-D-manno-heptose from D-glycero-beta-D-manno-heptose 7-phosphate: step 3/4.
Subunit structure	Homodimer By similarity.
Sequence similarities	In the N-terminal section; belongs to the carbohydrate kinase PfkB family. In the C-terminal section; belongs to the cytidylyltransferase family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological_process	ADP-L-glycero-beta-D-manno-heptose biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway carbohydrate phosphorylation Inferred from electronic annotation. Source: GOC
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP carbohydrate kinase activity Inferred from electronic annotation. Source: HAMAP nucleotidyltransferase activity Inferred from electronic annotation. Source: HAMAP phosphotransferase activity, alcohol group as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 476	476	Bifunctional protein HldE HAMAP-Rule MF_01603		PRO_0000291688
Regions
	Nucleotide binding	195 – 198	4	ATP Potential
	Region	1 – 319	319	Ribokinase HAMAP-Rule MF_01603
	Region	345 – 476	132	Cytidylyltransferase HAMAP-Rule MF_01603
Sites
	Active site	264	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q07Y78 [UniParc]. Last modified October 31, 2006. Version 1. Checksum: CFDA4E7393AA3515	FASTA	476	50,701
	10 20 30 40 50 60 MKVSLPAFEN ARVLVIGDVM LDRYWVGPTG RISPEAPVPV VKINQIEDRP GGAANVALNI 70 80 90 100 110 120 ATLGGHVQLA GIVGQDETAQ ALTQGVKVFG VEPQWLTVAD KPTITKLRVL SRNQQLIRLD 130 140 150 160 170 180 FEEQFDKATS QALFAQSEAI LDNVDVLVLS DYAKGAIDEP KDFIAKARAK GVKVLVDPKG 190 200 210 220 230 240 HDFARYHGAS LITPNMSEFE AVVGTVTSED DLIEKAQKLI KLHDFEAILV TRSEKGMTLV 250 260 270 280 290 300 SQNQPELHIP TVAREVHDVT GAGDTVISAL ATSIAAGASL AQACAIANTA AGVVVGKLGT 310 320 330 340 350 360 STVSRIELIQ ALALNHGESG FGVMTEDQLA YAMDQARLRG ERIVMTNGCF DILHAGHVSY 370 380 390 400 410 420 LQQAKALGDR LIVAVNDDSS VTRLKGPGRP VNPVDRRMAV LAGLASVDWV VPFTEDTPQR 430 440 450 460 470 IITRLLPDSL VKGGDYKVED IAGGAEVIAA GGKVEVLGFE DGVSTTAIIQ NIMSQK

References

[1]

"Complete sequence of Shewanella frigidimarina NCIMB 400." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Fredrickson J.K., Kolker E., McCuel L.A., DiChristina T., Nealson K.H., Newman D., Tiedje J.M. , Zhou J., Romine M.F., Culley D.E., Serres M., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCIMB 400.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000447 Genomic DNA. Translation: ABI73036.1.
RefSeq	YP_751875.1. NC_008345.1.
3D structure databases
ProteinModelPortal	Q07Y78.
ModBase	Search...
Protein-protein interaction databases
STRING	318167.Sfri_3200.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABI73036; ABI73036; Sfri_3200.
GeneID	4280102.
KEGG	sfr:Sfri_3200.
PATRIC	23500551. VBISheFri14343_3316.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2870.
HOGENOM	HOG000237584.
KO	K03272.
OMA	TKLRVMS.
ProtClustDB	PRK11316.
Enzyme and pathway databases
BioCyc	SFRI318167:GIXS-3314-MONOMER.
UniPathway	UPA00356; UER00437. UPA00356; UER00439.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
HAMAP	MF_01603. HldE.
InterPro	IPR023030. Bifunc_HldE. IPR002173. Carboh/pur_kinase_PfkB_CS. IPR004821. Cyt_trans-like. IPR011611. PfkB_dom. IPR011913. RfaE_dom_I. IPR011914. RfaE_dom_II. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Pfam	PF01467. CTP_transf_2. 1 hit. PF00294. PfkB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00125. cyt_tran_rel. 1 hit. TIGR02198. rfaE_dom_I. 1 hit. TIGR02199. rfaE_dom_II. 1 hit.
PROSITE	PS00583. PFKB_KINASES_1. 1 hit. PS00584. PFKB_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HLDE_SHEFN

Accession

Primary (citable) accession number: Q07Y78

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	October 31, 2006
Last modified:	May 1, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program