Reviewed, UniProtKB/Swiss-Prot Q07WU7 (FRDA_SHEFN)

Names and origin

Protein names

Recommended name:
    Fumarate reductase flavoprotein subunit
    EC=1.3.99.1
Alternative name(s):
    Flavocytochrome c
    Flavocytochrome c3
      Short name=Fcc3

Gene names

Name:	fccA
Synonyms:	fcc3
Ordered Locus Names:	Sfri_3690

Organism

Shewanella frigidimarina (strain NCIMB 400) [Complete proteome] [HAMAP]

Taxonomic identifier

318167 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	596 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes fumarate reduction using artificial electron donors such as methyl viologen. The physiological reductant is unknown, but evidence indicates that flavocytochrome c participates in electron transfer from formate to fumarate and possibly also to trimethylamine oxide (TMAO). This enzyme is essentially unidirectional By similarity.
Catalytic activity	Succinate + acceptor = fumarate + reduced acceptor.
Cofactor	Binds 1 FAD per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	PeriplasmBy similarity.
Induction	By anaerobiosis and fumarate.
Sequence similarities	In the C-terminal section; belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily. Contains 1 cytochrome c domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Periplasm
Domain	Signal
Ligand	FAD Flavoprotein Heme Iron Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW succinate dehydrogenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25
	Chain	26 – 596	571	Fumarate reductase flavoprotein subunit		PRO_0000269231
Regions
	Domain	26 – 142	117	Cytochrome c
	Nucleotide binding	154 – 168	15	FAD Potential
	Nucleotide binding	157 – 168	12	FAD By similarity
	Nucleotide binding	181 – 182	2	FAD By similarity
	Nucleotide binding	189 – 190	2	FAD By similarity
	Nucleotide binding	194 – 196	3	FAD By similarity
	Nucleotide binding	574 – 575	2	FAD By similarity
	Region	143 – 596	454	Flavoprotein-like
Sites
	Active site	427	1	Proton donor By similarity
	Metal binding	33	1	Iron (heme 2 axial ligand)
	Metal binding	43	1	Iron (heme 1 axial ligand)
	Metal binding	65	1	Iron (heme 2 axial ligand)
	Metal binding	83	1	Iron (heme 3 axial ligand)
	Metal binding	86	1	Iron (heme 4 axial ligand)
	Metal binding	97	1	Iron (heme 3 axial ligand)
	Metal binding	100	1	Iron (heme 1 axial ligand)
	Metal binding	111	1	Iron (heme 4 axial ligand)
	Binding site	39	1	Heme 1 (covalent)
	Binding site	42	1	Heme 1 (covalent)
	Binding site	61	1	Heme 2 (covalent)
	Binding site	64	1	Heme 2 (covalent)
	Binding site	93	1	Heme 3 (covalent)
	Binding site	96	1	Heme 3 (covalent)
	Binding site	107	1	Heme 4 (covalent)
	Binding site	110	1	Heme 4 (covalent)
	Binding site	390	1	Substrate By similarity
	Binding site	402	1	Substrate By similarity
	Binding site	529	1	Substrate By similarity
	Binding site	569	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q07WU7-1 [UniParc]. Last modified December 12, 2006. Version 2. Checksum: 914B69FD502BAC5B	FASTA	596	63,027
	10 20 30 40 50 60 MKKMNLAVCI ATLMGTAGLM GTAVAADNLA EFHVQNQECD SCHTPDGELS NDSLTYENTQ 70 80 90 100 110 120 CVSCHGTLAE VAETTKHEHY NAHASHFPGE VACTSCHSAH EKSMVYCDSC HSFDFNMPYA 130 140 150 160 170 180 KKWLRDEPTI AELAKDKSER QAALASAPHD TVDVVVVGSG GAGFSAAISA TDSGAKVILI 190 200 210 220 230 240 EKEPVIGGNA KLAAGGMNAA WTDQQKAKKI TDSPELMFED TMKGGQNIND PALVKVLSSH 250 260 270 280 290 300 SKDSVDWMTA MGADLTDVGM MGGASVNRAH RPTGGAGVGA HVVQVLYDNA VKRNIDLRMN 310 320 330 340 350 360 TRGIEVLKDD KGTVKGILVK GMYKGYYWVK ADAVILATGG FAKNNERVAK LDPSLKGFIS 370 380 390 400 410 420 TNQPGAVGDG LDVAENAGGA LKDMQYIQAH PTLSVKGGVM VTEAVRGNGA ILVNREGKRF 430 440 450 460 470 480 VNEITTRDKA SAAILAQTGK SAYLIFDDSV RKSLSKIDKY IGLGVAPTAD SLVKLGKMEG 490 500 510 520 530 540 IDGKALTETV ARYNSLVSSG KDTDFERPNL PRALNEGNYY AIEVTPGVHH TMGGVMIDTK 550 560 570 580 590 AEVMNAKKQV IPGLYGAGEV TGGVHGANRL GGNAISDIIT FGRLAGEEAA KYSKKN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of the gene encoding flavocytochrome c from Shewanella putrefaciens: a tetraheme flavoenzyme that is a soluble fumarate reductase related to the membrane-bound enzymes from other bacteria." Pealing S.L., Black A.C., Manson F.D.C., Ward F.B., Chapman S.K., Reid G.A. Biochemistry 31:12132-12140(1992) [PubMed: 1333793] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 26-38.
[2]	"Complete sequence of Shewanella frigidimarina NCIMB 400." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Fredrickson J.K., Kolker E., McCuel L.A., DiChristina T., Nealson K.H., Newman D., Tiedje J.M. , Zhou J., Romine M.F., Culley D.E., Serres M., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	L04283 Genomic DNA. Translation: AAA70385.1. CP000447 Genomic DNA. Translation: ABI73517.1. Different initiation.
RefSeq	YP_752356.1.
3D structure databases
SMR	Q07WU7. Positions 26-593.
ModBase	Search...
Genome annotation databases
GeneID	4280583.
GenomeReviews	Gene locus Sfri_3690 in contig CP000447_GR.
KEGG	sfr:Sfri_3690.
NMPDR	fig|318167.10.peg.3515.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q07WU7.
Family and domain databases
InterPro	IPR003953. FAD_bind2_N. IPR012286. Fcc3_N_cyt. IPR010960. Flavocytochrome_c. IPR011031. Multihaem_cyt. [Graphical view]
Gene3D	G3DSA:1.10.1130.10. Fcc3_N_cyt. 1 hit.
Pfam	PF00890. FAD_binding_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR01813. flavo_cyto_c. 1 hit.
PROSITE	PS51008. MULTIHEME_CYTC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FRDA_SHEFN

Accession

Primary (citable) accession number: Q07WU7
Secondary accession number(s): Q02469, Q9X969

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	December 12, 2006
Last modified:	November 25, 2008
This is version 18 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)