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Q07V74 (TRMB_RHOP5) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:RPE_0200
OrganismRhodopseudomonas palustris (strain BisA53) [Complete proteome] [HAMAP]
Taxonomic identifier316055 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeRhodopseudomonas

Protein attributes

Sequence length250 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 250250tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000288213

Sites

Active site1531 By similarity
Binding site791S-adenosyl-L-methionine By similarity
Binding site1041S-adenosyl-L-methionine By similarity
Binding site1311S-adenosyl-L-methionine By similarity
Binding site1531S-adenosyl-L-methionine By similarity
Binding site1571Substrate By similarity
Binding site1891Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q07V74 [UniParc].

Last modified October 31, 2006. Version 1.
Checksum: D21C4991F89731EF

FASTA25027,846
        10         20         30         40         50         60 
MTPDDPRDAS DASLADATAD SASRGHGSFF GRRKGHKLRA HQAELIADLL PQLSFDLAAP 

        70         80         90        100        110        120 
PPADLRALFE PGVDQVKLEI GFGGGEHLVA EAEAHPATGF IGCEPYVNGM AKILAQIEAR 

       130        140        150        160        170        180 
GIRNIRLFAG DAAELLAWAP KASLARIDLI HPDPWPKRRH WKRRFVQDAT VTAMARALRD 

       190        200        210        220        230        240 
DGEFRFVCDI DDYSAWTLSH LTRSPDFAWT AERARDWQNP FAGYTMTRYG RKAEREGRRA 

       250 
AYLHFQRNAA 

« Hide

References

[1]"Complete sequence of Rhodopseudomonas palustris BisA53."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Pelletier D.A., Kyrpides N., Kim E., Harwood C.S., Oda Y., Richardson P.
Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: BisA53.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000463 Genomic DNA. Translation: ABJ04160.1.
RefSeqYP_779140.1. NC_008435.1.

3D structure databases

ProteinModelPortalQ07V74.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING316055.RPE_0200.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABJ04160; ABJ04160; RPE_0200.
GeneID4358501.
KEGGrpe:RPE_0200.
PATRIC23254868. VBIRhoPal93214_0200.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073969.
KOK03439.
OMARAFYGRR.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycRPAL316055:GHR9-203-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_RHOP5
AccessionPrimary (citable) accession number: Q07V74
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: October 31, 2006
Last modified: June 11, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways