Q07T97 (LPXK_RHOP5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tetraacyldisaccharide 4'-kinase
EC=2.7.1.130

Alternative name(s):
Lipid A 4'-kinase

Gene names

Name:	lpxK
Ordered Locus Names:	RPE_0881

Organism

Rhodopseudomonas palustris (strain BisA53) [Complete proteome] [HAMAP]

Taxonomic identifier

316055 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA) By similarity. HAMAP MF_00409
Catalytic activity	ATP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate) = ADP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-4-O-phosphono-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate). HAMAP MF_00409
Pathway	Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 6/6. HAMAP MF_00409
Sequence similarities	Belongs to the LpxK family.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW tetraacyldisaccharide 4'-kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 340	340	Tetraacyldisaccharide 4'-kinase HAMAP MF_00409		PRO_0000291234
Regions
	Nucleotide binding	50 – 57	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q07T97 [UniParc]. Last modified October 31, 2006. Version 1. Checksum: 863490DBEE325277	FASTA	340	36,154
	10 20 30 40 50 60 MREPEFWRRR SLLSLLLMPL AALYGAIAAS RMRKPGRSIA VPVICVGNYH GGGAGKTPTT 70 80 90 100 110 120 LTLVALLREL DETPVVLSRG YGGSLNGPVE VDPDRHSAAE VGDEPLMMAR SVPVVVAKDR 130 140 150 160 170 180 VAGATLAMSR RASVIVMDDG FQNPALAKDV SLIVIDARRG IGNGRVIPSG PLRAPLPLQC 190 200 210 220 230 240 ERTDALLIIG AGSAADPVAA RLKSKGVPTL RGHLAPDPAC VEALRGRAVL AFAGIGDPER 250 260 270 280 290 300 FFATLRASGI EVAAERPFPD HHPYSGDDVA ALLDRARRDG LTLVTTEKDL ARLGDQPQLA 310 320 330 340 EAGLTALPVT LALEDEAGFR RFVIAQLGAA RDRRFRGNRR

References

[1]

"Complete sequence of Rhodopseudomonas palustris BisA53." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Pelletier D.A., Kyrpides N., Kim E., Harwood C.S., Oda Y., Richardson P. Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BisA53.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000463 Genomic DNA. Translation: ABJ04837.1.
RefSeq	YP_779817.1. NC_008435.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q07T97.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4360220.
GenomeReviews	Gene locus RPE_0881 in contig CP000463_GR.
KEGG	rpe:RPE_0881.
PATRIC	23256292. VBIRhoPal93214_0899.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1663.
HOGENOM	HBG663914.
OMA	VILMDDG.
ProtClustDB	PRK00652.
Enzyme and pathway databases
BioCyc	RPAL316055:RPE_0881-MONOMER.
Family and domain databases
HAMAP	MF_00409. LpxK. [Tree]
InterPro	IPR003758. Tetraacyldisaccharide_4-kinase. [Graphical view]
KO	K00912.
Pfam	PF02606. LpxK. 1 hit. [Graphical view]
TIGRFAMs	TIGR00682. LpxK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LPXK_RHOP5

Accession

Primary (citable) accession number: Q07T97

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 12, 2007
Last sequence update:	October 31, 2006
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program