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Protein

Light-independent protochlorophyllide reductase iron-sulfur ATP-binding protein

Gene

bchL

Organism
Rhodopseudomonas palustris (strain BisA53)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Component of the dark-operative protochlorophyllide reductase (DPOR) that uses Mg-ATP and reduced ferredoxin to reduce ring D of protochlorophyllide (Pchlide) to form chlorophyllide a (Chlide). This reaction is light-independent. The L component serves as a unique electron donor to the NB-component of the complex, and binds Mg-ATP.UniRule annotation

Catalytic activityi

Protochlorophyllide a + reduced ferredoxin + 2 ATP + 2 H2O = chlorophyllide a + oxidized ferredoxin + 2 ADP + 2 phosphate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per dimer.UniRule annotation

Pathwayi: bacteriochlorophyll biosynthesis (light-independent)

This protein is involved in the pathway bacteriochlorophyll biosynthesis (light-independent), which is part of Porphyrin-containing compound metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway bacteriochlorophyll biosynthesis (light-independent) and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi57MagnesiumUniRule annotation1
Binding sitei82ATPUniRule annotation1
Metal bindingi138Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation1
Metal bindingi172Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi53 – 58ATPUniRule annotation6
Nucleotide bindingi223 – 224ATPUniRule annotation2
Nucleotide bindingi247 – 249ATPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Bacteriochlorophyll biosynthesis, Chlorophyll biosynthesis, Photosynthesis

Keywords - Ligandi

4Fe-4S, ATP-binding, Iron, Iron-sulfur, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00671.

Names & Taxonomyi

Protein namesi
Recommended name:
Light-independent protochlorophyllide reductase iron-sulfur ATP-binding proteinUniRule annotation (EC:1.3.7.7UniRule annotation)
Short name:
DPOR subunit LUniRule annotation
Short name:
LI-POR subunit LUniRule annotation
Gene namesi
Name:bchLUniRule annotation
Ordered Locus Names:RPE_1350
OrganismiRhodopseudomonas palustris (strain BisA53)
Taxonomic identifieri316055 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeRhodopseudomonas
Proteomesi
  • UP000000654 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003240691 – 310Light-independent protochlorophyllide reductase iron-sulfur ATP-binding proteinAdd BLAST310

Interactioni

Subunit structurei

Homodimer. Protochlorophyllide reductase is composed of three subunits; BchL, BchN and BchB.UniRule annotation

Protein-protein interaction databases

STRINGi316055.RPE_1350.

Structurei

3D structure databases

ProteinModelPortaliQ07RY2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NifH/BchL/ChlL family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DSM. Bacteria.
COG1348. LUCA.
HOGENOMiHOG000228825.
KOiK04037.
OMAiTSCNISV.
OrthoDBiPOG091H0230.

Family and domain databases

CDDicd02032. Bchl_like. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00355. ChlL_BchL. 1 hit.
InterProiIPR030655. NifH/chlL_CS.
IPR000392. Nitogenase_NifH/Reductase_ChlL.
IPR027417. P-loop_NTPase.
IPR005971. Protochlorophyllide_ATP-bd.
[Graphical view]
PIRSFiPIRSF000363. Nitrogenase_iron. 1 hit.
PRINTSiPR00091. NITROGNASEII.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01281. DPOR_bchL. 1 hit.
PROSITEiPS00746. NIFH_FRXC_1. 1 hit.
PS00692. NIFH_FRXC_2. 1 hit.
PS51026. NIFH_FRXC_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q07RY2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNILTDPTKL QGCTDTNANK CVEGDGEGSV QVQLDPDLKI GTAKVFSIYG
60 70 80 90 100
KGGIGKSTTS SNLSVAFSKL GKRVLQIGCD PKHDSTFTLT KCLIPTVIDV
110 120 130 140 150
LESVNFHAEE LRPEDFVFEG YNGVMCLEAG GPPAGTGCGG YVVGQTVKLL
160 170 180 190 200
KEHHLLEDTD VVIFDVLGDV VCGGFAAPLQ HSDRAMIVTA NDFDSIFAAN
210 220 230 240 250
RIVAAITAKA KNYGVRVGGI IANRSDATDQ IDKFSERTGV PRVAHFPALD
260 270 280 290 300
IIRKSRLKKS TLFELDHSPE LAKVQEEYMR LATELWEGKQ PPCEGKALKD
310
REIFDLLGFD
Length:310
Mass (Da):33,548
Last modified:October 31, 2006 - v1
Checksum:iB757E286FC6868DE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000463 Genomic DNA. Translation: ABJ05302.1.
RefSeqiWP_011662788.1. NC_008435.1.

Genome annotation databases

EnsemblBacteriaiABJ05302; ABJ05302; RPE_1350.
KEGGirpe:RPE_1350.
PATRICi23257222. VBIRhoPal93214_1363.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000463 Genomic DNA. Translation: ABJ05302.1.
RefSeqiWP_011662788.1. NC_008435.1.

3D structure databases

ProteinModelPortaliQ07RY2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi316055.RPE_1350.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABJ05302; ABJ05302; RPE_1350.
KEGGirpe:RPE_1350.
PATRICi23257222. VBIRhoPal93214_1363.

Phylogenomic databases

eggNOGiENOG4105DSM. Bacteria.
COG1348. LUCA.
HOGENOMiHOG000228825.
KOiK04037.
OMAiTSCNISV.
OrthoDBiPOG091H0230.

Enzyme and pathway databases

UniPathwayiUPA00671.

Family and domain databases

CDDicd02032. Bchl_like. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00355. ChlL_BchL. 1 hit.
InterProiIPR030655. NifH/chlL_CS.
IPR000392. Nitogenase_NifH/Reductase_ChlL.
IPR027417. P-loop_NTPase.
IPR005971. Protochlorophyllide_ATP-bd.
[Graphical view]
PIRSFiPIRSF000363. Nitrogenase_iron. 1 hit.
PRINTSiPR00091. NITROGNASEII.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01281. DPOR_bchL. 1 hit.
PROSITEiPS00746. NIFH_FRXC_1. 1 hit.
PS00692. NIFH_FRXC_2. 1 hit.
PS51026. NIFH_FRXC_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBCHL_RHOP5
AccessioniPrimary (citable) accession number: Q07RY2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: October 31, 2006
Last modified: November 2, 2016
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.