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Q07PI4

- BIOB_RHOP5

UniProt

Q07PI4 - BIOB_RHOP5

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Protein

Biotin synthase

Gene

bioB

Organism
Rhodopseudomonas palustris (strain BisA53)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi66 – 661Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi70 – 701Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi73 – 731Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi110 – 1101Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi141 – 1411Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi201 – 2011Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi274 – 2741Iron-sulfur 2 (2Fe-2S)UniRule annotation

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciRPAL316055:GHR9-2226-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:RPE_2208
OrganismiRhodopseudomonas palustris (strain BisA53)
Taxonomic identifieri316055 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeRhodopseudomonas
ProteomesiUP000000654: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 338338Biotin synthasePRO_0000381581Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi316055.RPE_2208.

Structurei

3D structure databases

ProteinModelPortaliQ07PI4.
SMRiQ07PI4. Positions 17-326.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiDETQALC.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q07PI4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNSIDLPSLA EATPTIRHDW TREEAAAIYH APFADLMFRA QTIHRQSFDP
60 70 80 90 100
NAVQCNQLLN VKTGGCAEDC GYCSQSAHHD TALPASKLMD PKKVIEAAKA
110 120 130 140 150
ARDAGATRYC MGAAWREPKD RDMAPVIEMV KGVKALGMEA CVTLGMLSDA
160 170 180 190 200
QAKQLADAGL DYYNHNVDTS EEFYSQVVTT RSYGDRLDTL AKVHDAGIKV
210 220 230 240 250
CCGGILGLGE KPTDRVEMLR TLANLAQHPE SVPINMLIPI EGTPIAATAT
260 270 280 290 300
PVDPIEFVRT IALARIMMPK SDVRLAAGRT AMSDEMQALC FLAGANSIFI
310 320 330
GDTLLTTPNP GDSKDRQLFN RLGINARGEL GVHVHSHG
Length:338
Mass (Da):36,546
Last modified:October 31, 2006 - v1
Checksum:i605C632CAB20DFF3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000463 Genomic DNA. Translation: ABJ06150.1.
RefSeqiWP_011663632.1. NC_008435.1.
YP_781130.1. NC_008435.1.

Genome annotation databases

EnsemblBacteriaiABJ06150; ABJ06150; RPE_2208.
GeneIDi4359564.
KEGGirpe:RPE_2208.
PATRICi23258984. VBIRhoPal93214_2244.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000463 Genomic DNA. Translation: ABJ06150.1 .
RefSeqi WP_011663632.1. NC_008435.1.
YP_781130.1. NC_008435.1.

3D structure databases

ProteinModelPortali Q07PI4.
SMRi Q07PI4. Positions 17-326.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 316055.RPE_2208.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABJ06150 ; ABJ06150 ; RPE_2208 .
GeneIDi 4359564.
KEGGi rpe:RPE_2208.
PATRICi 23258984. VBIRhoPal93214_2244.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi DETQALC.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci RPAL316055:GHR9-2226-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BisA53.

Entry informationi

Entry nameiBIOB_RHOP5
AccessioniPrimary (citable) accession number: Q07PI4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: October 31, 2006
Last modified: October 29, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3