Q07MT5 (RISB_RHOP5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6,7-dimethyl-8-ribityllumazine synthase
Short name=DMRL synthase
Short name=Lumazine synthase
EC=2.5.1.9

Alternative name(s):
Riboflavin synthase beta chain

Gene names

Name:	ribH
Ordered Locus Names:	RPE_2812

Organism

Rhodopseudomonas palustris (strain BisA53) [Complete proteome] [HAMAP]

Taxonomic identifier

316055 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas

Protein attributes

Sequence length	163 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2-butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes the condensation of 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione with L-3,4-dihydrohy-2-butanone-4-phosphate yielding 6,7-dimethyl-8-lumazine By similarity. HAMAP MF_00178
Catalytic activity	2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine. HAMAP MF_00178
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 2/2. HAMAP MF_00178
Sequence similarities	Belongs to the DMRL synthase family.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	riboflavin synthase complex Inferred from electronic annotation. Source: InterPro
Molecular function	riboflavin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 163	163	6,7-dimethyl-8-ribityllumazine synthase HAMAP MF_00178		PRO_1000040500

Sequences

	Sequence		Length	Mass (Da)
	Q07MT5 [UniParc]. Last modified October 31, 2006. Version 1. Checksum: F1E8B0B5969BC7C5	FASTA	163	17,286
	10 20 30 40 50 60 MADPRRARLT DDTEIAGARA LIVEARFYDD IQDAMLEGAR AELTAAGLTH DVITVPGALE 70 80 90 100 110 120 IPAAIAIALD AAEAHGKPYD AVIALGCVVR GDTIHFEIVS MESSRALMDL AVSRKIPLGN 130 140 150 160 GIITVNTDEQ AWARAKATEL DKGGDAARAA LAMLRIKRRL AKG

References

[1]

"Complete sequence of Rhodopseudomonas palustris BisA53." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Pelletier D.A., Kyrpides N., Kim E., Harwood C.S., Oda Y., Richardson P. Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BisA53.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000463 Genomic DNA. Translation: ABJ06749.1.
RefSeq	YP_781729.1. NC_008435.1.
3D structure databases
ProteinModelPortal	Q07MT5.
SMR	Q07MT5. Positions 19-161.
ModBase	Search...
Protein-protein interaction databases
STRING	Q07MT5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4360495.
GenomeReviews	Gene locus RPE_2812 in contig CP000463_GR.
KEGG	rpe:RPE_2812.
PATRIC	23260214. VBIRhoPal93214_2847.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0054.
HOGENOM	HBG311126.
OMA	SSRALMD.
ProtClustDB	PRK00061.
Enzyme and pathway databases
BioCyc	RPAL316055:RPE_2812-MONOMER.
Family and domain databases
HAMAP	MF_00178. Lumazine_synth. [Tree]
InterPro	IPR002180. DMRL_synthase. [Graphical view]
Gene3D	G3DSA:3.40.50.960. DMRL_synthase. 1 hit.
KO	K00794.
PANTHER	PTHR21058. DMRL_synthase. 1 hit.
Pfam	PF00885. DMRL_synthase. 1 hit. [Graphical view]
SUPFAM	SSF52121. DMRL_synthase. 1 hit.
TIGRFAMs	TIGR00114. Lumazine-synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RISB_RHOP5

Accession

Primary (citable) accession number: Q07MT5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 31, 2006
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program