Q07L51 (BIOD_RHOP5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent dethiobiotin synthetase BioD
EC=6.3.3.3

Alternative name(s):
DTB synthetase
Short name=DTBS
Dethiobiotin synthase

Gene names

Name:	bioD
Ordered Locus Names:	RPE_3401

Organism

Rhodopseudomonas palustris (strain BisA53) [Complete proteome] [HAMAP]

Taxonomic identifier

316055 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas

Protein attributes

Sequence length	213 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336
Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity. HAMAP MF_00336
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Subcellular location	Cytoplasm By similarity HAMAP MF_00336.
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW dethiobiotin synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 213	213	ATP-dependent dethiobiotin synthetase BioD HAMAP MF_00336		PRO_0000302544
Regions
	Nucleotide binding	100 – 103	4	ATP By similarity
	Nucleotide binding	184 – 186	3	ATP By similarity
Sites
	Metal binding	17	1	Magnesium 2 By similarity
	Metal binding	100	1	Magnesium 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q07L51 [UniParc]. Last modified October 31, 2006. Version 1. Checksum: DC71474CBA80E730	FASTA	213	22,598
	10 20 30 40 50 60 MSPRVVVTGT DTGIGKTVFS AALVGALDGV YWKPVQAGLD EETDRDTVLR LSGLPAERML 70 80 90 100 110 120 PEAYRLNTPA SPHLAAEIDG VTIDPAKLAL PQHDRPLVVE GAGGLLTPLT RSVSYIDVIA 130 140 150 160 170 180 SWHAPVVLCA RTTLGTINHS LLSIEALRAR DIELLGIAFI GEAHAESENI IAAMGQVRRL 190 200 210 GRLPHLASLT TDSLKAAFAD AFNIGDFFKD ATA

References

[1]

"Complete sequence of Rhodopseudomonas palustris BisA53." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Pelletier D.A., Kyrpides N., Kim E., Harwood C.S., Oda Y., Richardson P. Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BisA53.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000463 Genomic DNA. Translation: ABJ07333.1.
RefSeq	YP_782313.1. NC_008435.1.
3D structure databases
ProteinModelPortal	Q07L51.
ModBase	Search...
Protein-protein interaction databases
STRING	Q07L51.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4361087.
GenomeReviews	Gene locus RPE_3401 in contig CP000463_GR.
KEGG	rpe:RPE_3401.
PATRIC	23261410. VBIRhoPal93214_3442.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0132.
HOGENOM	HBG650065.
OMA	WKPIQSG.
ProtClustDB	PRK00090.
Enzyme and pathway databases
BioCyc	RPAL316055:RPE_3401-MONOMER.
Family and domain databases
HAMAP	MF_00336. BioD. [Tree]
InterPro	IPR004472. DTB_synth_BioD. [Graphical view]
KO	K01935.
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. BioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_RHOP5

Accession

Primary (citable) accession number: Q07L51

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	October 31, 2006
Last modified:	January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program