Q07K87 (GLGB_RHOP5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB
EC=2.4.1.18

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE

Gene names

Name:	glgB
Ordered Locus Names:	RPE_3718

Organism

Rhodopseudomonas palustris (strain BisA53) [Complete proteome] [HAMAP]

Taxonomic identifier

316055 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas › Rhodopseudomonas palustris

Protein attributes

Sequence length	715 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis Glycogen metabolism
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 715	715	1,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685		PRO_1000044995
Sites
	Active site	399	1	Nucleophile By similarity
	Active site	452	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q07K87 [UniParc]. Last modified October 31, 2006. Version 1. Checksum: 9ADFEEC7426C8194	FASTA	715	80,859
	10 20 30 40 50 60 MIATLPDEAY AIVEGRHADP FRYLGPHREN ERTVVRAFLP EAASVEAVDE KGGTAALERI 70 80 90 100 110 120 HDAGLFAGLL PNGSARYQLR ARFGDTIVDL EDPYRFPPIL TDFDLYLLGE GSHQRLYDKL 130 140 150 160 170 180 GAHLMTLDGV EGVGFVVLAP NARRVSVVGD FNFWDSRRHP MRVRGSGYWE LFIPRATAGD 190 200 210 220 230 240 HYKFEIVSAQ GHLLPLKSDP MAFAAEMRPS TASIVLDPER VPHPRPAPAG ISALTSPMSI 250 260 270 280 290 300 YEVHLGSWRR KDNNDWLSYR ELAEQLPAYV RDLGFTHVEF LPINEHPFDG SWGYQPTGLF 310 320 330 340 350 360 APTSRFGTPE DFAALVDACH AHGLGVLLDW VPGHFPDDPH GLAHFDGTSL YEHANPLQGR 370 380 390 400 410 420 HNDWGTLIYN YGRTEVVNFL VSNALFWLER YAVDGLRVDA VASMLYLDYS RPAGGWIPNK 430 440 450 460 470 480 YGGRENLEAI DFLRRFNTEV FAKFPNATTA AEESTAWPQV SQPVEFGGLG FGYKWNMGWM 490 500 510 520 530 540 HDTLNYISMD PIYRKYHHGQ ILFGLHYAFS ENFILPLSHD EVVHGKRSIL GRMPGDHWQR 550 560 570 580 590 600 FANLRAYYAF MFAHPGKKLM FMGCEFGQDR EWNHDSSLDW HLLEQESHRG VQSVVRDLNN 610 620 630 640 650 660 LYRTMPALYE LDCDSFGFEW IVTDDGDRNV FAWIRKGNAA RARCLVIANF SPNVYYDYQV 670 680 690 700 710 RVPFPGKWRE VFNSDSAHYG GSNVGNIGEV EAVGLVPELS LTIPPLAVIF LTPED

References

[1]

"Complete sequence of Rhodopseudomonas palustris BisA53." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Pelletier D.A., Kyrpides N., Kim E., Harwood C.S., Oda Y., Richardson P. Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BisA53.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000463 Genomic DNA. Translation: ABJ07647.1.
RefSeq	YP_782627.1. NC_008435.1.
3D structure databases
ProteinModelPortal	Q07K87.
SMR	Q07K87. Positions 112-714.
ModBase	Search...
Protein-protein interaction databases
STRING	316055.RPE_3718.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABJ07647; ABJ07647; RPE_3718.
GeneID	4361410.
KEGG	rpe:RPE_3718.
PATRIC	23262054. VBIRhoPal93214_3758.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0296.
HOGENOM	HOG000283037.
KO	K00700.
OMA	HFITMAL.
ProtClustDB	PRK05402.
Enzyme and pathway databases
BioCyc	RPAL316055:GHR9-3773-MONOMER.
UniPathway	UPA00164.
Family and domain databases
Gene3D	2.60.40.10. 2 hits. 2.60.40.1180. 1 hit. 3.20.20.80. 1 hit.
HAMAP	MF_00685. GlgB.
InterPro	IPR006407. 1-4-A-glucan_branch_enz. IPR006048. A-amylase_b_C. IPR015902. Glyco_hydro_13. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
PANTHER	PTHR10357. PTHR10357. 1 hit. PTHR10357:SF13. PTHR10357:SF13. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 2 hits.
TIGRFAMs	TIGR01515. branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB_RHOP5

Accession

Primary (citable) accession number: Q07K87

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 31, 2006
Last modified:	May 1, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program