ID   GFA_RHOP5               Reviewed;         189 AA.
AC   Q07HI5;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2006, sequence version 1.
DT   16-JUN-2009, entry version 19.
DE   RecName: Full=Glutathione-dependent formaldehyde-activating enzyme;
DE            EC=4.4.1.22;
DE   AltName: Full=S-(hydroxymethyl)glutathione synthase;
GN   Name=gfa; OrderedLocusNames=RPE_4679;
OS   Rhodopseudomonas palustris (strain BisA53).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Bradyrhizobiaceae; Rhodopseudomonas.
OX   NCBI_TaxID=316055;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H.,
RA   Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Pelletier D.A., Kyrpides N., Kim E.,
RA   Harwood C.S., Oda Y., Richardson P.;
RT   "Complete sequence of Rhodopseudomonas palustris BisA53.";
RL   Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the condensation of formaldehyde and
CC       glutathione to S-hydroxymethylglutathione (By similarity).
CC   -!- CATALYTIC ACTIVITY: S-(hydroxymethyl)glutathione = glutathione +
CC       formaldehyde.
CC   -!- COFACTOR: Binds 2 zinc ions per subunit (By similarity).
CC   -!- PATHWAY: One-carbon metabolism; formaldehyde degradation; formate
CC       from formaldehyde (glutathione route): step 1/3.
CC   -!- SIMILARITY: Belongs to the gfa family.
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DR   EMBL; CP000463; ABJ08599.1; -; Genomic_DNA.
DR   RefSeq; YP_783579.1; -.
DR   GeneID; 4362382; -.
DR   GenomeReviews; CP000463_GR; RPE_4679.
DR   KEGG; rpe:RPE_4679; -.
DR   HOGENOM; Q07HI5; -.
DR   OMA; Q07HI5; HACGCTK.
DR   GO; GO:0051907; F:S-(hydroxymethyl)glutathione synthase activity; IEA:HAMAP.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0046294; P:formaldehyde catabolic process; IEA:HAMAP.
DR   HAMAP; MF_00723; -; 1.
DR   InterPro; IPR014185; Formald_GSH.
DR   InterPro; IPR006913; GFA.
DR   Pfam; PF04828; GFA; 1.
DR   PIRSF; PIRSF033318; Formald_GSH; 1.
DR   TIGRFAMs; TIGR02820; formald_GSH; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Lyase; Metal-binding; Zinc.
FT   CHAIN         1    189       Glutathione-dependent formaldehyde-
FT                                activating enzyme.
FT                                /FTId=PRO_1000045839.
FT   METAL        27     27       Zinc 1; structural (By similarity).
FT   METAL        29     29       Zinc 1; structural (By similarity).
FT   METAL        48     48       Zinc 2; catalytic (By similarity).
FT   METAL        50     50       Zinc 2; catalytic (By similarity).
FT   METAL        53     53       Zinc 2; catalytic (By similarity).
FT   METAL        95     95       Zinc 1; structural (By similarity).
FT   METAL        98     98       Zinc 1; structural (By similarity).
SQ   SEQUENCE   189 AA;  20117 MW;  858B0B557F13BD13 CRC64;
     MTVHIHPAVD NGVKKGTGSF AGGTLVCKCS DRPVKVGIKG DVAHNHACGC TKCWKPDGAT
     FSVVAVVPRG NVTVLENGDK LQIVDTSATI QRHACKVCGT HMFGRIENTG HPFYGLDFIH
     PELFYEQGSA APEFAAFVSS IIESGVDPAQ MPEIRSRLKE LGLEPYDCLS PALMDAIAGH
     IAKTSRKAA
//