Reviewed, UniProtKB/Swiss-Prot Q07HI5 (GFA_RHOP5)

Names and origin

Protein names

Recommended name:
    Glutathione-dependent formaldehyde-activating enzyme
    EC=4.4.1.22
Alternative name(s):
    S-(hydroxymethyl)glutathione synthase

Gene names

Name:	gfa
Ordered Locus Names:	RPE_4679

Organism

Rhodopseudomonas palustris (strain BisA53) [Complete proteome] [HAMAP]

Taxonomic identifier

316055 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas

Protein attributes

Sequence length	189 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the condensation of formaldehyde and glutathione to S-hydroxymethylglutathione By similarity.
Catalytic activity	S-(hydroxymethyl)glutathione = glutathione + formaldehyde. HAMAP MF_00723
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	One-carbon metabolism; formaldehyde degradation; formate from formaldehyde (glutathione route): step 1/3. HAMAP MF_00723
Sequence similarities	Belongs to the gfa family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	formaldehyde catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	S-(hydroxymethyl)glutathione synthase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 189	189	Glutathione-dependent formaldehyde-activating enzyme HAMAP MF_00723		PRO_1000045839
Sites
	Metal binding	27	1	Zinc 1; structural By similarity
	Metal binding	29	1	Zinc 1; structural By similarity
	Metal binding	48	1	Zinc 2; catalytic By similarity
	Metal binding	50	1	Zinc 2; catalytic By similarity
	Metal binding	53	1	Zinc 2; catalytic By similarity
	Metal binding	95	1	Zinc 1; structural By similarity
	Metal binding	98	1	Zinc 1; structural By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q07HI5-1 [UniParc]. Last modified October 31, 2006. Version 1. Checksum: 858B0B557F13BD13	FASTA	189	20,117
	10 20 30 40 50 60 MTVHIHPAVD NGVKKGTGSF AGGTLVCKCS DRPVKVGIKG DVAHNHACGC TKCWKPDGAT 70 80 90 100 110 120 FSVVAVVPRG NVTVLENGDK LQIVDTSATI QRHACKVCGT HMFGRIENTG HPFYGLDFIH 130 140 150 160 170 180 PELFYEQGSA APEFAAFVSS IIESGVDPAQ MPEIRSRLKE LGLEPYDCLS PALMDAIAGH IAKTSRKAA

References

[1]

"Complete sequence of Rhodopseudomonas palustris BisA53." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Pelletier D.A., Kyrpides N., Kim E., Harwood C.S., Oda Y., Richardson P. Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000463 Genomic DNA. Translation: ABJ08599.1.
RefSeq	YP_783579.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4362382.
GenomeReviews	Gene locus RPE_4679 in contig CP000463_GR.
KEGG	rpe:RPE_4679.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q07HI5.
OMA	Q07HI5. HACGCTK.
Family and domain databases
HAMAP	MF_00723. [Tree]
InterPro	IPR014185. Formald_GSH. IPR006913. GFA. [Graphical view]
Pfam	PF04828. GFA. 1 hit. [Graphical view]
PIRSF	PIRSF033318. Formald_GSH. 1 hit.
TIGRFAMs	TIGR02820. formald_GSH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GFA_RHOP5

Accession

Primary (citable) accession number: Q07HI5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 31, 2006
Last modified:	June 16, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)