true2008-01-152024-03-2792RUVC_RHOP5Complete sequence of Rhodopseudomonas palustris BisA53.Copeland A.Lucas S.Lapidus A.Barry K.Detter J.C.Glavina del Rio T.Hammon N.Israni S.Dalin E.Tice H.Pitluck S.Chain P.Malfatti S.Shin M.Vergez L.Schmutz J.Larimer F.Land M.Hauser L.Pelletier D.A.Kyrpides N.Kim E.Harwood C.S.Oda Y.Richardson P.US DOE Joint Genome Institute2006-09EMBL/GenBank/DDBJNUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]BisA53BacteriaRuvCRibonuclease H-like superfamily/Ribonuclease HRuvCRNaseH-like_sfRNaseH_sfX-over_junc_endoDNase_Mg_BSX-over_junc_endoDNase_RuvCruvCCROSSOVER JUNCTION ENDODEOXYRIBONUCLEASE RUVCCROSSOVER JUNCTION ENDODEOXYRIBONUCLEASE RUVCRuvCRSOLVASERUVCRibonuclease H-likeRUVCCrossover junction endodeoxyribonuclease RuvC3.1.21.10Holliday junction nuclease RuvCHolliday junction resolvase RuvCruvCRPE_4770The RuvA-RuvB-RuvC complex processes Holliday junction (HJ) DNA during genetic recombination and DNA repair. Endonuclease that resolves HJ intermediates. Cleaves cruciform DNA by making single-stranded nicks across the HJ at symmetrical positions within the homologous arms, yielding a 5'-phosphate and a 3'-hydroxyl group; requires a central core of homology in the junction. The consensus cleavage sequence is 5'-(A/T)TT(C/G)-3'. Cleavage occurs on the 3'-side of the TT dinucleotide at the point of strand exchange. HJ branch migration catalyzed by RuvA-RuvB allows RuvC to scan DNA until it finds its consensus sequence, where it cleaves and resolves the cruciform DNA.Binds 2 Mg(2+) ion per subunit.Homodimer which binds Holliday junction (HJ) DNA. The HJ becomes 2-fold symmetrical on binding to RuvC with unstacked arms; it has a different conformation from HJ DNA in complex with RuvA. In the full resolvosome a probable DNA-RuvA(4)-RuvB(12)-RuvC(2) complex forms which resolves the HJ.Belongs to the RuvC family.Crossover junction endodeoxyribonuclease RuvC1821011741676148122006-10-31118210e349b8600542d8a3e24dc035c22774cfMTQPPNRQPVRILGIDPGLRRTGWGVIESEGNRLVFIGCGSVEPPDTLPLAERLLAIHRGLAKVLADFAPDEAAVEQTFVNKDAGATLKLGQARGVAMLAPAMVGLSVAEYAPNLVKKTVVGAGHADKNQIQMMLKILLPKAEPPSADAADALAVAITHAHHRVAAARLKAVGAtrue