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Protein

NADPH--cytochrome P450 reductase

Gene
N/A
Organism
Musca domestica (House fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.UniRule annotation

Catalytic activityi

NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • FADUniRule annotationNote: Binds 1 FAD per monomer.UniRule annotation
  • FMNUniRule annotationNote: Binds 1 FMN per monomer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei205 – 2051FMNUniRule annotation
Binding sitei295 – 2951NADPUniRule annotation
Binding sitei475 – 4751FADUniRule annotation
Binding sitei529 – 5291NADPUniRule annotation
Binding sitei632 – 6321NADPUniRule annotation
Binding sitei670 – 6701FADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi83 – 886FMNUniRule annotation
Nucleotide bindingi135 – 1384FMNUniRule annotation
Nucleotide bindingi170 – 17910FMNUniRule annotation
Nucleotide bindingi451 – 4544FADUniRule annotation
Nucleotide bindingi469 – 4713FADUniRule annotation
Nucleotide bindingi485 – 4884FADUniRule annotation
Nucleotide bindingi589 – 5902NADPUniRule annotation
Nucleotide bindingi595 – 5995NADPUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, FMN, NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-18499.

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH--cytochrome P450 reductaseUniRule annotation (EC:1.6.2.4UniRule annotation)
Short name:
CPRUniRule annotation
Short name:
P450RUniRule annotation
OrganismiMusca domestica (House fly)
Taxonomic identifieri7370 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaMuscoideaMuscidaeMusca

Subcellular locationi

  • Endoplasmic reticulum membrane UniRule annotation; Single-pass membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1414LumenalUniRule annotationAdd
BLAST
Transmembranei15 – 3521HelicalUniRule annotationAdd
BLAST
Topological domaini36 – 671636CytoplasmicUniRule annotationAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 671671NADPH--cytochrome P450 reductasePRO_0000167603Add
BLAST

Expressioni

Developmental stagei

Expressed throughout development, with high levels before metamorphosis and low levels in pupae.

Structurei

3D structure databases

ProteinModelPortaliQ07994.
SMRiQ07994. Positions 64-229.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini77 – 221145Flavodoxin-likeUniRule annotationAdd
BLAST
Domaini276 – 515240FAD-binding FR-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the NADPH--cytochrome P450 reductase family.UniRule annotation
In the N-terminal section; belongs to the flavodoxin family.UniRule annotation
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.UniRule annotation
Contains 1 FAD-binding FR-type domain.UniRule annotation
Contains 1 flavodoxin-like domain.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

KOiK00327.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
HAMAPiMF_03212. NCPR.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like_dom.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q07994-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAEHVEEVV SEEPFLGTLD IALLVVLLVG ATWYFMRSRK KEEAPIRSYS
60 70 80 90 100
IQPTTVSTVS TTENSFIKKL KASGRSLVVF YGSQTGTAEE FAGRLAKEGL
110 120 130 140 150
RYRMKGMVAD PEECDMEELL QMKDIPNSLA VFCLATYGEG DPTDNAMEFY
160 170 180 190 200
EWITNGEVDL TGLNYAVFGL GNKTYEHYNK VAIYVDKRLE ELGATRVFEL
210 220 230 240 250
GLGDDDANIE DDFITWKDRF WPSVCDFFGI EGSGEEVLMR QFRLLEQPDV
260 270 280 290 300
QPDRIYTGEI ARLHSMQNQR PPFDAKNPFL ASVIVNRELH KGGGRSCMHI
310 320 330 340 350
ELDIDGSKMR YDAGDHIAMY PINDKILVEK LGKLCDANLD TVFSLINTDT
360 370 380 390 400
DSSKKHPFPC PTTYRTALTH YLEITAIPRT HILKELAEYC SDEKDKEFLR
410 420 430 440 450
NMASITPEGK EKYQNWIQNS SRNIVHILED IKSCRPPIDH ICELLPRLQP
460 470 480 490 500
RYYSISSSSK LYPTNVHITA VLVQYETPTG RVNKGVATSY MKEKNPSVGE
510 520 530 540 550
VKVPVFIRKS QFRLPTKSEI PIIMVGPGTG LAPFRGFIQE RQFLRDGGKV
560 570 580 590 600
VGDTILYFGC RKKDEDFIYR EELEQYVQNG TLTLKTAFSR DQQEKIYVTH
610 620 630 640 650
LIEQDADLIW KVIGEQKGHF YICGDAKNMA VDVRNILVKI LSTKGNMNES
660 670
DAVQYIKKME AQKRYSADVW S
Length:671
Mass (Da):76,358
Last modified:October 1, 1994 - v1
Checksum:iA00A2C35DFD8D129
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19897 mRNA. Translation: AAA29295.1.
PIRiA56592.
RefSeqiNP_001273818.1. NM_001286889.1.
XP_011294253.1. XM_011295951.1.

Genome annotation databases

GeneIDi101890161.
KEGGimde:101890161.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19897 mRNA. Translation: AAA29295.1.
PIRiA56592.
RefSeqiNP_001273818.1. NM_001286889.1.
XP_011294253.1. XM_011295951.1.

3D structure databases

ProteinModelPortaliQ07994.
SMRiQ07994. Positions 64-229.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi101890161.
KEGGimde:101890161.

Organism-specific databases

CTDi33883.

Phylogenomic databases

KOiK00327.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-18499.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
HAMAPiMF_03212. NCPR.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin-like.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like_dom.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The cDNA and deduced protein sequence of house fly NADPH-cytochrome P450 reductase."
    Koener J.F., Carino F.A., Feyereisen R.
    Insect Biochem. Mol. Biol. 23:439-447(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Rutgers.
    Tissue: Abdomen.

Entry informationi

Entry nameiNCPR_MUSDO
AccessioniPrimary (citable) accession number: Q07994
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: January 20, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.