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Q07994

- NCPR_MUSDO

UniProt

Q07994 - NCPR_MUSDO

Protein

NADPH--cytochrome P450 reductase

Gene
N/A
Organism
Musca domestica (House fly)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 1 (01 Oct 1994)
      Previous versions | rss
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    Functioni

    This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.

    Catalytic activityi

    NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.

    Cofactori

    FAD.
    FMN.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi167 – 19832FMNPROSITE-ProRule annotationAdd
    BLAST
    Nucleotide bindingi311 – 32212FADBy similarityAdd
    BLAST
    Nucleotide bindingi448 – 45811FADBy similarityAdd
    BLAST
    Nucleotide bindingi524 – 54219NADPBy similarityAdd
    BLAST
    Nucleotide bindingi618 – 63417NADPBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. FMN binding Source: InterPro
    2. iron ion binding Source: InterPro
    3. NADPH-hemoprotein reductase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein, FMN, NADP

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-18499.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADPH--cytochrome P450 reductase (EC:1.6.2.4)
    Short name:
    CPR
    Short name:
    P450R
    OrganismiMusca domestica (House fly)
    Taxonomic identifieri7370 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaMuscoideaMuscidaeMusca

    Subcellular locationi

    Endoplasmic reticulum membrane; Peripheral membrane protein
    Note: Anchored to the ER membrane by its N-terminal hydrophobic region.

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 671671NADPH--cytochrome P450 reductasePRO_0000167603Add
    BLAST

    Expressioni

    Developmental stagei

    Expressed throughout development, with high levels before metamorphosis and low levels in pupae.

    Structurei

    3D structure databases

    ProteinModelPortaliQ07994.
    SMRiQ07994. Positions 64-229.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini77 – 221145Flavodoxin-likePROSITE-ProRule annotationAdd
    BLAST
    Domaini276 – 515240FAD-binding FR-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.Curated
    Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
    Contains 1 flavodoxin-like domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di1.20.990.10. 1 hit.
    3.40.50.360. 1 hit.
    InterProiIPR003097. FAD-binding_1.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001094. Flavdoxin.
    IPR008254. Flavodoxin/NO_synth.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR029039. Flavoprotein-like.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR023208. P450R.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view]
    PfamiPF00667. FAD_binding_1. 1 hit.
    PF00258. Flavodoxin_1. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000208. P450R. 1 hit.
    PRINTSiPR00369. FLAVODOXIN.
    PR00371. FPNCR.
    SUPFAMiSSF52218. SSF52218. 1 hit.
    SSF63380. SSF63380. 1 hit.
    PROSITEiPS51384. FAD_FR. 1 hit.
    PS50902. FLAVODOXIN_LIKE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q07994-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSAEHVEEVV SEEPFLGTLD IALLVVLLVG ATWYFMRSRK KEEAPIRSYS    50
    IQPTTVSTVS TTENSFIKKL KASGRSLVVF YGSQTGTAEE FAGRLAKEGL 100
    RYRMKGMVAD PEECDMEELL QMKDIPNSLA VFCLATYGEG DPTDNAMEFY 150
    EWITNGEVDL TGLNYAVFGL GNKTYEHYNK VAIYVDKRLE ELGATRVFEL 200
    GLGDDDANIE DDFITWKDRF WPSVCDFFGI EGSGEEVLMR QFRLLEQPDV 250
    QPDRIYTGEI ARLHSMQNQR PPFDAKNPFL ASVIVNRELH KGGGRSCMHI 300
    ELDIDGSKMR YDAGDHIAMY PINDKILVEK LGKLCDANLD TVFSLINTDT 350
    DSSKKHPFPC PTTYRTALTH YLEITAIPRT HILKELAEYC SDEKDKEFLR 400
    NMASITPEGK EKYQNWIQNS SRNIVHILED IKSCRPPIDH ICELLPRLQP 450
    RYYSISSSSK LYPTNVHITA VLVQYETPTG RVNKGVATSY MKEKNPSVGE 500
    VKVPVFIRKS QFRLPTKSEI PIIMVGPGTG LAPFRGFIQE RQFLRDGGKV 550
    VGDTILYFGC RKKDEDFIYR EELEQYVQNG TLTLKTAFSR DQQEKIYVTH 600
    LIEQDADLIW KVIGEQKGHF YICGDAKNMA VDVRNILVKI LSTKGNMNES 650
    DAVQYIKKME AQKRYSADVW S 671
    Length:671
    Mass (Da):76,358
    Last modified:October 1, 1994 - v1
    Checksum:iA00A2C35DFD8D129
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L19897 mRNA. Translation: AAA29295.1.
    PIRiA56592.
    RefSeqiNP_001273818.1. NM_001286889.1.

    Genome annotation databases

    GeneIDi101890161.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L19897 mRNA. Translation: AAA29295.1 .
    PIRi A56592.
    RefSeqi NP_001273818.1. NM_001286889.1.

    3D structure databases

    ProteinModelPortali Q07994.
    SMRi Q07994. Positions 64-229.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 101890161.

    Organism-specific databases

    CTDi 33883.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-18499.

    Family and domain databases

    Gene3Di 1.20.990.10. 1 hit.
    3.40.50.360. 1 hit.
    InterProi IPR003097. FAD-binding_1.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001094. Flavdoxin.
    IPR008254. Flavodoxin/NO_synth.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR029039. Flavoprotein-like.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR023208. P450R.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view ]
    Pfami PF00667. FAD_binding_1. 1 hit.
    PF00258. Flavodoxin_1. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000208. P450R. 1 hit.
    PRINTSi PR00369. FLAVODOXIN.
    PR00371. FPNCR.
    SUPFAMi SSF52218. SSF52218. 1 hit.
    SSF63380. SSF63380. 1 hit.
    PROSITEi PS51384. FAD_FR. 1 hit.
    PS50902. FLAVODOXIN_LIKE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The cDNA and deduced protein sequence of house fly NADPH-cytochrome P450 reductase."
      Koener J.F., Carino F.A., Feyereisen R.
      Insect Biochem. Mol. Biol. 23:439-447(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Rutgers.
      Tissue: Abdomen.

    Entry informationi

    Entry nameiNCPR_MUSDO
    AccessioniPrimary (citable) accession number: Q07994
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3