Reviewed, UniProtKB/Swiss-Prot Q07994 (NCPR_MUSDO)

Names and origin

Protein names	Recommended name:     NADPH--cytochrome P450 reductase       Short name=CPR       Short name=P450R     EC=1.6.2.4
Organism	Musca domestica (House fly)
Taxonomic identifier	7370 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Muscoidea › Muscidae › Musca

Protein attributes

Sequence length	671 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.
Catalytic activity	NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.
Cofactor	FAD. FMN.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Note: Anchored to the ER membrane by its N-terminal hydrophobic region.
Developmental stage	Expressed throughout development, with high levels before metamorphosis and low levels in pupae.
Sequence similarities	In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain. Contains 1 flavodoxin-like domain.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Ligand	FAD FMN Flavoprotein NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FMN binding Inferred from electronic annotation. Source: InterPro NADPH-hemoprotein reductase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 671	671	NADPH--cytochrome P450 reductase		PRO_0000167603
Regions
	Domain	77 – 221	145	Flavodoxin-like
	Domain	276 – 515	240	FAD-binding FR-type
	Nucleotide binding	167 – 198	32	FMN By similarity
	Nucleotide binding	311 – 322	12	FAD By similarity
	Nucleotide binding	448 – 458	11	FAD By similarity
	Nucleotide binding	524 – 542	19	NADP By similarity
	Nucleotide binding	618 – 634	17	NADP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q07994-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: A00A2C35DFD8D129	FASTA	671	76,358
	10 20 30 40 50 60 MSAEHVEEVV SEEPFLGTLD IALLVVLLVG ATWYFMRSRK KEEAPIRSYS IQPTTVSTVS 70 80 90 100 110 120 TTENSFIKKL KASGRSLVVF YGSQTGTAEE FAGRLAKEGL RYRMKGMVAD PEECDMEELL 130 140 150 160 170 180 QMKDIPNSLA VFCLATYGEG DPTDNAMEFY EWITNGEVDL TGLNYAVFGL GNKTYEHYNK 190 200 210 220 230 240 VAIYVDKRLE ELGATRVFEL GLGDDDANIE DDFITWKDRF WPSVCDFFGI EGSGEEVLMR 250 260 270 280 290 300 QFRLLEQPDV QPDRIYTGEI ARLHSMQNQR PPFDAKNPFL ASVIVNRELH KGGGRSCMHI 310 320 330 340 350 360 ELDIDGSKMR YDAGDHIAMY PINDKILVEK LGKLCDANLD TVFSLINTDT DSSKKHPFPC 370 380 390 400 410 420 PTTYRTALTH YLEITAIPRT HILKELAEYC SDEKDKEFLR NMASITPEGK EKYQNWIQNS 430 440 450 460 470 480 SRNIVHILED IKSCRPPIDH ICELLPRLQP RYYSISSSSK LYPTNVHITA VLVQYETPTG 490 500 510 520 530 540 RVNKGVATSY MKEKNPSVGE VKVPVFIRKS QFRLPTKSEI PIIMVGPGTG LAPFRGFIQE 550 560 570 580 590 600 RQFLRDGGKV VGDTILYFGC RKKDEDFIYR EELEQYVQNG TLTLKTAFSR DQQEKIYVTH 610 620 630 640 650 660 LIEQDADLIW KVIGEQKGHF YICGDAKNMA VDVRNILVKI LSTKGNMNES DAVQYIKKME 670 AQKRYSADVW S

References

[1]	"The cDNA and deduced protein sequence of house fly NADPH-cytochrome P450 reductase." Koener J.F., Carino F.A., Feyereisen R. Insect Biochem. Mol. Biol. 23:439-447(1993) [PubMed: 8508186] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Rutgers. Tissue: Abdomen.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L19897 mRNA. Translation: AAA29295.1.
PIR	A56592.
3D structure databases
HSSP	HSSP built from PDB template 1AMO based on UniProtKB P00388.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.6.2.4. 21508.
Family and domain databases
InterPro	IPR003097. FAD-binding_1. IPR017927. Fd_Rdtase_FAD-bd. IPR001094. Flavdoxin-like. IPR008254. Flavodoxin/NO_synth. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR015702. NADPH_Cyt_P450_Rdtase. IPR001433. OxRdtase_FAD/NAD_bd. [Graphical view]
PANTHER	PTHR19384:SF17. NADPH_Cyt_Red. 1 hit.
Pfam	PF00667. FAD_binding_1. 1 hit. PF00258. Flavodoxin_1. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00369. FLAVODOXIN. PR00371. FPNCR.
PROSITE	PS51384. FAD_FR. 1 hit. PS50902. FLAVODOXIN_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NCPR_MUSDO

Accession

Primary (citable) accession number: Q07994

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)