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Q07994

- NCPR_MUSDO

UniProt

Q07994 - NCPR_MUSDO

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Protein
NADPH--cytochrome P450 reductase
Gene
N/A
Organism
Musca domestica (House fly)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.

Catalytic activityi

NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.

Cofactori

FAD.
FMN.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi167 – 19832FMN By similarity
Add
BLAST
Nucleotide bindingi311 – 32212FAD By similarity
Add
BLAST
Nucleotide bindingi448 – 45811FAD By similarity
Add
BLAST
Nucleotide bindingi524 – 54219NADP By similarity
Add
BLAST
Nucleotide bindingi618 – 63417NADP By similarity
Add
BLAST

GO - Molecular functioni

  1. FMN binding Source: InterPro
  2. NADPH-hemoprotein reductase activity Source: UniProtKB-EC
  3. iron ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, FMN, NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-18499.

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH--cytochrome P450 reductase (EC:1.6.2.4)
Short name:
CPR
Short name:
P450R
OrganismiMusca domestica (House fly)
Taxonomic identifieri7370 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaMuscoideaMuscidaeMusca

Subcellular locationi

Endoplasmic reticulum membrane; Peripheral membrane protein
Note: Anchored to the ER membrane by its N-terminal hydrophobic region.

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 671671NADPH--cytochrome P450 reductase
PRO_0000167603Add
BLAST

Expressioni

Developmental stagei

Expressed throughout development, with high levels before metamorphosis and low levels in pupae.

Structurei

3D structure databases

ProteinModelPortaliQ07994.
SMRiQ07994. Positions 64-229.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini77 – 221145Flavodoxin-like
Add
BLAST
Domaini276 – 515240FAD-binding FR-type
Add
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q07994-1 [UniParc]FASTAAdd to Basket

« Hide

MSAEHVEEVV SEEPFLGTLD IALLVVLLVG ATWYFMRSRK KEEAPIRSYS    50
IQPTTVSTVS TTENSFIKKL KASGRSLVVF YGSQTGTAEE FAGRLAKEGL 100
RYRMKGMVAD PEECDMEELL QMKDIPNSLA VFCLATYGEG DPTDNAMEFY 150
EWITNGEVDL TGLNYAVFGL GNKTYEHYNK VAIYVDKRLE ELGATRVFEL 200
GLGDDDANIE DDFITWKDRF WPSVCDFFGI EGSGEEVLMR QFRLLEQPDV 250
QPDRIYTGEI ARLHSMQNQR PPFDAKNPFL ASVIVNRELH KGGGRSCMHI 300
ELDIDGSKMR YDAGDHIAMY PINDKILVEK LGKLCDANLD TVFSLINTDT 350
DSSKKHPFPC PTTYRTALTH YLEITAIPRT HILKELAEYC SDEKDKEFLR 400
NMASITPEGK EKYQNWIQNS SRNIVHILED IKSCRPPIDH ICELLPRLQP 450
RYYSISSSSK LYPTNVHITA VLVQYETPTG RVNKGVATSY MKEKNPSVGE 500
VKVPVFIRKS QFRLPTKSEI PIIMVGPGTG LAPFRGFIQE RQFLRDGGKV 550
VGDTILYFGC RKKDEDFIYR EELEQYVQNG TLTLKTAFSR DQQEKIYVTH 600
LIEQDADLIW KVIGEQKGHF YICGDAKNMA VDVRNILVKI LSTKGNMNES 650
DAVQYIKKME AQKRYSADVW S 671
Length:671
Mass (Da):76,358
Last modified:October 1, 1994 - v1
Checksum:iA00A2C35DFD8D129
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L19897 mRNA. Translation: AAA29295.1.
PIRiA56592.
RefSeqiNP_001273818.1. NM_001286889.1.

Genome annotation databases

GeneIDi101890161.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L19897 mRNA. Translation: AAA29295.1 .
PIRi A56592.
RefSeqi NP_001273818.1. NM_001286889.1.

3D structure databases

ProteinModelPortali Q07994.
SMRi Q07994. Positions 64-229.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 101890161.

Organism-specific databases

CTDi 33883.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-18499.

Family and domain databases

Gene3Di 1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
InterProi IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view ]
Pfami PF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view ]
PIRSFi PIRSF000208. P450R. 1 hit.
PRINTSi PR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMi SSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEi PS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The cDNA and deduced protein sequence of house fly NADPH-cytochrome P450 reductase."
    Koener J.F., Carino F.A., Feyereisen R.
    Insect Biochem. Mol. Biol. 23:439-447(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Rutgers.
    Tissue: Abdomen.

Entry informationi

Entry nameiNCPR_MUSDO
AccessioniPrimary (citable) accession number: Q07994
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: July 9, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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