ID   XYL2_YEAST              Reviewed;         356 AA.
AC   Q07993;
DT   09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   16-JUN-2009, entry version 56.
DE   RecName: Full=D-xylulose reductase;
DE            EC=1.1.1.9;
DE   AltName: Full=Xylitol dehydrogenase;
DE            Short=XDH;
GN   Name=XYL2; OrderedLocusNames=YLR070C;
OS   Saccharomyces cerevisiae (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=4932;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204511 / S288c / AB972;
RX   MEDLINE=97313267; PubMed=9169871;
RA   Johnston M., Hillier L.W., Riles L., Albermann K., Andre B.,
RA   Ansorge W., Benes V., Brueckner M., Delius H., Dubois E.,
RA   Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U.,
RA   Heumann K., Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K.,
RA   Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T.,
RA   Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E.,
RA   Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M.,
RA   Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C.,
RA   Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M.,
RA   Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H.,
RA   Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A.,
RA   Hani J., Hoheisel J.D.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL   Nature 387:87-90(1997).
RN   [2]
RP   INDUCTION, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX   PubMed=10486580; DOI=10.1016/S0014-5793(99)01016-9;
RA   Richard P., Toivari M.H., Penttilae M.;
RT   "Evidence that the gene YLR070c of Saccharomyces cerevisiae encodes a
RT   xylitol dehydrogenase.";
RL   FEBS Lett. 457:135-138(1999).
CC   -!- CATALYTIC ACTIVITY: Xylitol + NAD(+) = D-xylulose + NADH.
CC   -!- COFACTOR: Binds 1 zinc ion per subunit (By similarity).
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=1.1 mM for D-xylose;
CC         KM=240 uM for NADH;
CC         KM=25 mM for xylitol;
CC         KM=100 uM for NAD;
CC   -!- PATHWAY: Carbohydrate degradation; L-arabinose degradation via L-
CC       arabinitol; D-xylulose 5-phosphate from L-arabinose (fungi route):
CC       step 4/5.
CC   -!- INDUCTION: By xylose.
CC   -!- SIMILARITY: Belongs to the zinc-containing alcohol dehydrogenase
CC       family.
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DR   EMBL; Z73242; CAA97627.1; -; Genomic_DNA.
DR   PIR; S64902; S64902.
DR   RefSeq; NP_013171.1; -.
DR   HSSP; Q00796; 1PL7.
DR   DIP; DIP:4533N; -.
DR   IntAct; Q07993; 4.
DR   Ensembl; YLR070C; Saccharomyces cerevisiae.
DR   GeneID; 850759; -.
DR   GenomeReviews; Y13138_GR; YLR070C.
DR   KEGG; sce:YLR070C; -.
DR   NMPDR; fig|4932.3.peg.4162; -.
DR   CYGD; YLR070c; -.
DR   SGD; S000004060; XYL2.
DR   HOGENOM; Q07993; -.
DR   OMA; Q07993; THGRIAN.
DR   BRENDA; 1.1.1.9; 250.
DR   NextBio; 966906; -.
DR   GO; GO:0046526; F:D-xylulose reductase activity; IDA:SGD.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0042732; P:D-xylose metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0005999; P:xylulose biosynthetic process; IDA:SGD.
DR   InterPro; IPR013154; ADH_GroES-like.
DR   InterPro; IPR002085; ADH_SF_Zn.
DR   InterPro; IPR013149; ADH_Zn-bd.
DR   InterPro; IPR002328; ADH_Zn_CS.
DR   PANTHER; PTHR11695; ADH_Sf_Zn; 1.
DR   Pfam; PF08240; ADH_N; 1.
DR   Pfam; PF00107; ADH_zinc_N; 1.
DR   ProDom; PD040557; GroES_related; 1.
DR   PROSITE; PS00059; ADH_ZINC; 1.
PE   1: Evidence at protein level;
KW   Carbohydrate metabolism; Complete proteome; Metal-binding; NAD;
KW   Oxidoreductase; Xylose metabolism; Zinc.
FT   CHAIN         1    356       D-xylulose reductase.
FT                                /FTId=PRO_0000270925.
FT   NP_BIND     179    184       NAD (Potential).
FT   METAL        44     44       Zinc; catalytic (By similarity).
FT   METAL        69     69       Zinc; catalytic (By similarity).
FT   METAL       155    155       Zinc; catalytic (By similarity).
SQ   SEQUENCE   356 AA;  38600 MW;  5DFD19BDB2E0AE00 CRC64;
     MTDLTTQEAI VLERPGKITL TNVSIPKISD PNEVIIQIKA TGICGSDIHY YTHGRIANYV
     VESPMVLGHE SSGIVALIGE NVKTLKVGDR VALEPGIPDR FSPEMKEGRY NLDPNLKFAA
     TPPFDGTLTK YYKTMKDFVY KLPDDVSFEE GALIEPLSVA IHANKLAKIK FGARCVVFGA
     GPIGLLAGKV ASVFGAADVV FVDLLENKLE TARQFGATHI VNSGDLPHGV TVDSVIKKAI
     GKKGADVVFE CSGAEPCVRA GIEVCKAGGT IVQVGMGQEE IQFPISIIPT KELTFQGCFR
     YCQGDYSDSI ELVSSRKLSL KPFITHRYSF KDAVEAFEET SHHPLNNIKT IIEGPE
//