Q07993 (XYL2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
D-xylulose reductase
EC=1.1.1.9

Alternative name(s):
Xylitol dehydrogenase
Short name=XDH

Gene names

Name:	XYL2
Ordered Locus Names:	YLR070C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Xylitol + NAD+ = D-xylulose + NADH.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Carbohydrate degradation; L-arabinose degradation via L-arabinitol; D-xylulose 5-phosphate from L-arabinose (fungal route): step 4/5.
Induction	By xylose. Ref.3
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family.
Biophysicochemical properties	Kinetic parameters: KM=1.1 mM for D-xylose Ref.3 KM=240 µM for NADH KM=25 mM for xylitol KM=100 µM for NAD

Ontologies

Keywords
Biological process	Carbohydrate metabolism Xylose metabolism
Ligand	Metal-binding NAD Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	D-xylose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW xylulose biosynthetic process Inferred from direct assay Ref.3. Source: SGD
Molecular function	D-xylulose reductase activity Inferred from direct assay Ref.3. Source: SGD nucleotide binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 356	356	D-xylulose reductase		PRO_0000270925
Regions
	Nucleotide binding	179 – 184	6	NAD Potential
Sites
	Metal binding	44	1	Zinc; catalytic By similarity
	Metal binding	69	1	Zinc; catalytic By similarity
	Metal binding	155	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q07993 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 5DFD19BDB2E0AE00	FASTA	356	38,600
	10 20 30 40 50 60 MTDLTTQEAI VLERPGKITL TNVSIPKISD PNEVIIQIKA TGICGSDIHY YTHGRIANYV 70 80 90 100 110 120 VESPMVLGHE SSGIVALIGE NVKTLKVGDR VALEPGIPDR FSPEMKEGRY NLDPNLKFAA 130 140 150 160 170 180 TPPFDGTLTK YYKTMKDFVY KLPDDVSFEE GALIEPLSVA IHANKLAKIK FGARCVVFGA 190 200 210 220 230 240 GPIGLLAGKV ASVFGAADVV FVDLLENKLE TARQFGATHI VNSGDLPHGV TVDSVIKKAI 250 260 270 280 290 300 GKKGADVVFE CSGAEPCVRA GIEVCKAGGT IVQVGMGQEE IQFPISIIPT KELTFQGCFR 310 320 330 340 350 YCQGDYSDSI ELVSSRKLSL KPFITHRYSF KDAVEAFEET SHHPLNNIKT IIEGPE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Evidence that the gene YLR070c of Saccharomyces cerevisiae encodes a xylitol dehydrogenase." Richard P., Toivari M.H., Penttilae M. FEBS Lett. 457:135-138(1999) [PubMed: 10486580] [Abstract] Cited for: INDUCTION, BIOPHYSICOCHEMICAL PROPERTIES.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z73242 Genomic DNA. Translation: CAA97627.1. BK006945 Genomic DNA. Translation: DAA09387.1.
PIR	S64902.
RefSeq	NP_013171.1. NM_001181957.1.
3D structure databases
HSSP	HSSP built from PDB template 1PL8 based on UniProtKB Q00796.
ProteinModelPortal	Q07993.
SMR	Q07993. Positions 5-356.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4533N.
IntAct	Q07993. 2 interactions.
MINT	MINT-499230.
STRING	Q07993.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR070C; YLR070C; YLR070C.
GeneID	850759.
KEGG	sce:YLR070C.
NMPDR	fig|4932.3.peg.4162.
Organism-specific databases
CYGD	YLR070c.
SGD	S000004060. XYL2.
Phylogenomic databases
eggNOG	fuNOG06615.
GeneTree	EFGT00050000001794.
HOGENOM	HBG753318.
OMA	THGRIAN.
OrthoDB	EOG4SFDFJ.
Gene expression databases
ArrayExpress	Q07993.
Genevestigator	Q07993.
Family and domain databases
InterPro	IPR013149. ADH_C. IPR013154. ADH_GroES-like. IPR002085. ADH_SF_Zn-type. IPR002328. ADH_Zn_CS. IPR011032. GroES-like. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K05351.
PANTHER	PTHR11695. ADH_Sf_Zn. 1 hit.
Pfam	PF08240. ADH_N. 1 hit. PF00107. ADH_zinc_N. 1 hit. [Graphical view]
SUPFAM	SSF50129. GroES_like. 1 hit.
PROSITE	PS00059. ADH_ZINC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	966906.

Entry information

Entry name

XYL2_YEAST

Accession

Primary (citable) accession number: Q07993
Secondary accession number(s): D6VY71

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program