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Protein

D-xylulose reductase

Gene

XYL2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Xylitol + NAD+ = D-xylulose + NADH.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Kineticsi

  1. KM=1.1 mM for D-xylose1 Publication
  2. KM=240 µM for NADH1 Publication
  3. KM=25 mM for xylitol1 Publication
  4. KM=100 µM for NAD1 Publication

    Pathwayi: L-arabinose degradation via L-arabinitol

    This protein is involved in step 4 of the subpathway that synthesizes D-xylulose 5-phosphate from L-arabinose (fungal route).
    Proteins known to be involved in the 5 steps of the subpathway in this organism are:
    1. no protein annotated in this organism
    2. no protein annotated in this organism
    3. no protein annotated in this organism
    4. D-xylulose reductase (XYL2)
    5. no protein annotated in this organism
    This subpathway is part of the pathway L-arabinose degradation via L-arabinitol, which is itself part of Carbohydrate degradation.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-xylulose 5-phosphate from L-arabinose (fungal route), the pathway L-arabinose degradation via L-arabinitol and in Carbohydrate degradation.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi44Zinc; catalyticBy similarity1
    Metal bindingi69Zinc; catalyticBy similarity1
    Metal bindingi155Zinc; catalyticBy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi179 – 184NADSequence analysis6

    GO - Molecular functioni

    • D-xylulose reductase activity Source: SGD
    • zinc ion binding Source: InterPro

    GO - Biological processi

    Keywordsi

    Molecular functionOxidoreductase
    Biological processCarbohydrate metabolism, Xylose metabolism
    LigandMetal-binding, NAD, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:YLR070C-MONOMER.
    UniPathwayiUPA00146; UER00577.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    D-xylulose reductase (EC:1.1.1.9)
    Alternative name(s):
    Xylitol dehydrogenase
    Short name:
    XDH
    Gene namesi
    Name:XYL2
    Ordered Locus Names:YLR070C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome XII

    Organism-specific databases

    EuPathDBiFungiDB:YLR070C.
    SGDiS000004060. XYL2.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002709251 – 356D-xylulose reductaseAdd BLAST356

    Proteomic databases

    PRIDEiQ07993.

    Expressioni

    Inductioni

    By xylose.1 Publication

    Interactioni

    Protein-protein interaction databases

    BioGridi31344. 71 interactors.
    DIPiDIP-4533N.
    IntActiQ07993. 2 interactors.
    MINTiMINT-499230.

    Structurei

    3D structure databases

    ProteinModelPortaliQ07993.
    SMRiQ07993.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    GeneTreeiENSGT00550000074781.
    HOGENOMiHOG000294670.
    InParanoidiQ07993.
    KOiK05351.
    OMAiTDVHIYH.
    OrthoDBiEOG092D29X6.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.180.10. 1 hit.
    InterProiView protein in InterPro
    IPR013149. ADH_C.
    IPR013154. ADH_N.
    IPR002328. ADH_Zn_CS.
    IPR011032. GroES-like.
    IPR016040. NAD(P)-bd_dom.
    IPR020843. PKS_ER.
    PfamiView protein in Pfam
    PF08240. ADH_N. 1 hit.
    PF00107. ADH_zinc_N. 1 hit.
    SMARTiView protein in SMART
    SM00829. PKS_ER. 1 hit.
    SUPFAMiSSF50129. SSF50129. 1 hit.
    SSF51735. SSF51735. 1 hit.
    PROSITEiView protein in PROSITE
    PS00059. ADH_ZINC. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q07993-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTDLTTQEAI VLERPGKITL TNVSIPKISD PNEVIIQIKA TGICGSDIHY
    60 70 80 90 100
    YTHGRIANYV VESPMVLGHE SSGIVALIGE NVKTLKVGDR VALEPGIPDR
    110 120 130 140 150
    FSPEMKEGRY NLDPNLKFAA TPPFDGTLTK YYKTMKDFVY KLPDDVSFEE
    160 170 180 190 200
    GALIEPLSVA IHANKLAKIK FGARCVVFGA GPIGLLAGKV ASVFGAADVV
    210 220 230 240 250
    FVDLLENKLE TARQFGATHI VNSGDLPHGV TVDSVIKKAI GKKGADVVFE
    260 270 280 290 300
    CSGAEPCVRA GIEVCKAGGT IVQVGMGQEE IQFPISIIPT KELTFQGCFR
    310 320 330 340 350
    YCQGDYSDSI ELVSSRKLSL KPFITHRYSF KDAVEAFEET SHHPLNNIKT

    IIEGPE
    Length:356
    Mass (Da):38,600
    Last modified:November 1, 1996 - v1
    Checksum:i5DFD19BDB2E0AE00
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Z73242 Genomic DNA. Translation: CAA97627.1.
    BK006945 Genomic DNA. Translation: DAA09387.1.
    PIRiS64902.
    RefSeqiNP_013171.1. NM_001181957.1.

    Genome annotation databases

    EnsemblFungiiYLR070C; YLR070C; YLR070C.
    GeneIDi850759.
    KEGGisce:YLR070C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Z73242 Genomic DNA. Translation: CAA97627.1.
    BK006945 Genomic DNA. Translation: DAA09387.1.
    PIRiS64902.
    RefSeqiNP_013171.1. NM_001181957.1.

    3D structure databases

    ProteinModelPortaliQ07993.
    SMRiQ07993.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi31344. 71 interactors.
    DIPiDIP-4533N.
    IntActiQ07993. 2 interactors.
    MINTiMINT-499230.

    Proteomic databases

    PRIDEiQ07993.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiYLR070C; YLR070C; YLR070C.
    GeneIDi850759.
    KEGGisce:YLR070C.

    Organism-specific databases

    EuPathDBiFungiDB:YLR070C.
    SGDiS000004060. XYL2.

    Phylogenomic databases

    GeneTreeiENSGT00550000074781.
    HOGENOMiHOG000294670.
    InParanoidiQ07993.
    KOiK05351.
    OMAiTDVHIYH.
    OrthoDBiEOG092D29X6.

    Enzyme and pathway databases

    UniPathwayiUPA00146; UER00577.
    BioCyciYEAST:YLR070C-MONOMER.

    Miscellaneous databases

    PROiQ07993.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.180.10. 1 hit.
    InterProiView protein in InterPro
    IPR013149. ADH_C.
    IPR013154. ADH_N.
    IPR002328. ADH_Zn_CS.
    IPR011032. GroES-like.
    IPR016040. NAD(P)-bd_dom.
    IPR020843. PKS_ER.
    PfamiView protein in Pfam
    PF08240. ADH_N. 1 hit.
    PF00107. ADH_zinc_N. 1 hit.
    SMARTiView protein in SMART
    SM00829. PKS_ER. 1 hit.
    SUPFAMiSSF50129. SSF50129. 1 hit.
    SSF51735. SSF51735. 1 hit.
    PROSITEiView protein in PROSITE
    PS00059. ADH_ZINC. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiXYL2_YEAST
    AccessioniPrimary (citable) accession number: Q07993
    Secondary accession number(s): D6VY71
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2007
    Last sequence update: November 1, 1996
    Last modified: February 15, 2017
    This is version 125 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.