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Protein

GTPase KRas

Gene

KRAS

Organism
Monodelphis domestica (Gray short-tailed opossum)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (By similarity). Plays an important role in the regulation of cell proliferation (PubMed:8312604). Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner (By similarity).By similarity1 Publication

Enzyme regulationi

Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP). Interaction with SOS1 promotes exchange of bound GDP by GTP.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 189GTPBy similarity
Nucleotide bindingi29 – 357GTPBy similarity
Nucleotide bindingi59 – 602GTPBy similarity
Nucleotide bindingi116 – 1194GTPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
GTPase KRas
Alternative name(s):
K-Ras 2
Ki-Ras
c-K-ras
c-Ki-ras
Cleaved into the following chain:
Gene namesi
Name:KRAS
OrganismiMonodelphis domestica (Gray short-tailed opossum)
Taxonomic identifieri13616 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaMetatheriaDidelphimorphiaDidelphidaeMonodelphis
Proteomesi
  • UP000002280 Componenti: Unplaced

Subcellular locationi

  • Cell membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity
  • Cytoplasmcytosol By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Involvement in diseasei

Mutation which changes position 61 is implicated in ultraviolet radiation-induced (UVR-induced) eye tumor.

Keywords - Diseasei

Disease mutation, Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 185185GTPase KRasPRO_0000082642Add
BLAST
Initiator methionineiRemoved; alternateBy similarity
Chaini2 – 185184GTPase KRas, N-terminally processedPRO_0000326481Add
BLAST
Propeptidei186 – 1883Removed in mature formBy similarityPRO_0000281292

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei2 – 21N-acetylthreonine; in GTPase KRas, N-terminally processedBy similarity
Modified residuei104 – 1041N6-acetyllysineBy similarity
Modified residuei118 – 1181S-nitrosocysteineBy similarity
Modified residuei185 – 1851Cysteine methyl esterBy similarity
Lipidationi185 – 1851S-farnesyl cysteineBy similarity

Post-translational modificationi

Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs).By similarity

Keywords - PTMi

Acetylation, Lipoprotein, Methylation, Prenylation, S-nitrosylation

Interactioni

Subunit structurei

Interacts with PHLPP. Interacts (active GTP-bound form preferentially) with RGS14 (By similarity). Interacts (when farnesylated) with PDE6D; this promotes dissociation from the cell membrane (By similarity). Interacts with SOS1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi13616.ENSMODP00000021787.

Structurei

3D structure databases

ProteinModelPortaliQ07983.
SMRiQ07983. Positions 1-166.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni166 – 18520Hypervariable regionAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi32 – 409Effector region

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ras family.Curated

Phylogenomic databases

eggNOGiKOG0395. Eukaryota.
COG1100. LUCA.
HOGENOMiHOG000233973.
HOVERGENiHBG009351.
InParanoidiQ07983.
KOiK07827.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q07983-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET
60 70 80 90 100
CLLDILDTAG QEEYSAMRDQ YMRTGEGFLC VFAINNTKSF EDIHHYREQI
110 120 130 140 150
KRVKDSEDVP MVLVGNKCDL PSRTVDTKQA QDLARSYGIP FIETSAKTRQ
160 170 180
GGDDAFYTLV REIRKHKEKM SKDGKKKKKK SKTKCIIM
Length:188
Mass (Da):21,396
Last modified:November 1, 1995 - v1
Checksum:iB1B6C319A03F29D1
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti61 – 611Q → L in UVR-induced corneal tumor.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z12125 mRNA. Translation: CAA78108.1.
PIRiS31720.
RefSeqiNP_001028153.1. NM_001032981.2.
UniGeneiMdm.233.

Genome annotation databases

GeneIDi554186.
KEGGimdo:554186.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z12125 mRNA. Translation: CAA78108.1.
PIRiS31720.
RefSeqiNP_001028153.1. NM_001032981.2.
UniGeneiMdm.233.

3D structure databases

ProteinModelPortaliQ07983.
SMRiQ07983. Positions 1-166.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi13616.ENSMODP00000021787.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi554186.
KEGGimdo:554186.

Organism-specific databases

CTDi3845.

Phylogenomic databases

eggNOGiKOG0395. Eukaryota.
COG1100. LUCA.
HOGENOMiHOG000233973.
HOVERGENiHBG009351.
InParanoidiQ07983.
KOiK07827.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Characterization of the K-ras gene of the marsupial Monodelphis domestica."
    Kusewitt D.F., Kelly G., Sabourin C.L.K., Ley R.D.
    DNA Seq. 4:37-42(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Eye.

Entry informationi

Entry nameiRASK_MONDO
AccessioniPrimary (citable) accession number: Q07983
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 6, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.