Q07980 (MLH2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA mismatch repair protein MLH2

Alternative name(s):
MutL protein homolog 2

Gene names

Name:	MLH2
Ordered Locus Names:	YLR035C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	695 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in DNA mismatch repair (MMR), correcting insertion-deletion loops (IDLs) resulting from DNA replication, DNA damage or from recombination events between non-identical sequences during meiosis. Component of a MutL heterodimer with MLH1, which probably forms a ternary complex with the MutSbeta heterodimer that initially recognizes the DNA mismatches. This complex is thought to be responsible for directing the downsteam MMR events, including strand discrimination, excision, and resynthesis. Plays a role in the repair of heteroduplex sites present in meiotic recombination intermediates and is involved in maintaining the genetic stability of simple sequence repeats by corection of frameshift intermediates. Ref.3 Ref.4 Ref.7
Subunit structure	Heterodimer of MLH1 and MLH2.
Subcellular location	Nucleus Ref.5.
Miscellaneous	Present with 72 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the DNA mismatch repair mutL/hexB family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Nucleus
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	mismatch repair Inferred from mutant phenotype Ref.4. Source: SGD reciprocal meiotic recombination Inferred from mutant phenotype Ref.7. Source: SGD
Cellular component	MutLalpha complex Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro ATPase activity Inferred from Biological aspect of Ancestor. Source: RefGenome mismatched DNA binding Inferred from Biological aspect of Ancestor. Source: RefGenome protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MLH1	P38920	5	EBI-33369,EBI-11003

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 695	695	DNA mismatch repair protein MLH2		PRO_0000245569

Sequences

	Sequence		Length	Mass (Da)
	Q07980 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: DA5DA6AC86603A81	FASTA	695	78,248
	10 20 30 40 50 60 MTIHQLSPES QWKIVSSSFI YGPVAAVREL LDNSIDSGAK KVFIDVDSTT GGCEYISVKD 70 80 90 100 110 120 DGSGVDIIDR PSMCLEYTTS KMSSLGDISI LTTLGFRGEA LFLLSNLCNQ KGSMQVETKT 130 140 150 160 170 180 ADDVIGEKWL VDSKGGITNG KRYKVSCPVG TTVILRKLLG GLRARYLEIS SRPRKTFDEL 190 200 210 220 230 240 IYLINHYSLI HRNIRFYFSL VSLQKNGAIE RKQMQETLDP KISRARSLSL LARLKKPVPL 250 260 270 280 290 300 NFIVEENFVI DEKINLDLIL PRMVPESDVI NIKRRFKFLS VNERALSLNL ETGKTISKLL 310 320 330 340 350 360 SSIYRDFSLL DPMVWFINLN CDTKLLDVNI EPEKNDVMIK SFEVIFKKIE NKLKLLLEER 370 380 390 400 410 420 IGIETNMLGD KHVQPSINEK TSPALVIPTP DAENEISKGC GAVSGKDKTD IPQKNSDLIV 430 440 450 460 470 480 PTFYDEANLE NTTIVAATPS PTKFSEDKAL DEQTQLTISS YRSSSSGSMA SEDSTNWRHN 490 500 510 520 530 540 FQQELSENSE VAGAGSSTLP SSLTYNYIET IPENEDLELS KDASISNPFM ITKIRNVNKK 550 560 570 580 590 600 LSENLLEAKR TSCGDANKRG MPNERQLSNT EKKELIKLQQ TYGKRNNTVD MTIPRNSKKK 610 620 630 640 650 660 VTDNYIKKAS CMHKTRPKLM HFSEYTNNYV YTLKNEKIVK HDSDNFAKET LWLRSRDDAT 670 680 690 SPSSSLLQAL RAHVKKPGHI EATTNEWCLF TPDSP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Functional specificity of MutL homologs in yeast: evidence for three Mlh1-based heterocomplexes with distinct roles during meiosis in recombination and mismatch correction." Wang T.-F., Kleckner N., Hunter N. Proc. Natl. Acad. Sci. U.S.A. 96:13914-13919(1999) [PubMed: 10570173] [Abstract] Cited for: FUNCTION, INTERACTION WITH MLH1.
[4]	"Discrete in vivo roles for the MutL homologs Mlh2p and Mlh3p in the removal of frameshift intermediates in budding yeast." Harfe B.D., Minesinger B.K., Jinks-Robertson S. Curr. Biol. 10:145-148(2000) [PubMed: 10679328] [Abstract] Cited for: FUNCTION.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"A role for the MutL homologue MLH2 in controlling heteroduplex formation and in regulating between two different crossover pathways in budding yeast." Abdullah M.F.F., Hoffmann E.R., Cotton V.E., Borts R.H. Cytogenet. Genome Res. 107:180-190(2004) [PubMed: 15467363] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z73207 Genomic DNA. Translation: CAA97559.1. BK006945 Genomic DNA. Translation: DAA09353.1.
PIR	S64862.
RefSeq	NP_013135.1. NM_001181922.1.
3D structure databases
ProteinModelPortal	Q07980.
SMR	Q07980. Positions 22-77.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2413N.
IntAct	Q07980. 7 interactions.
MINT	MINT-502091.
STRING	Q07980.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR035C; YLR035C; YLR035C.
GeneID	850722.
KEGG	sce:YLR035C.
NMPDR	fig|4932.3.peg.4126.
Organism-specific databases
CYGD	YLR035c.
SGD	S000004025. MLH2.
Phylogenomic databases
eggNOG	fuNOG08190.
GeneTree	EFGT00050000002852.
OrthoDB	EOG4B5SFH.
Gene expression databases
ArrayExpress	Q07980.
Genevestigator	Q07980.
GermOnline	YLR035C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003594. ATPase-like_ATP-bd. IPR002099. DNA_mismatch_repair. IPR013507. DNA_mismatch_repair_C. IPR014762. DNA_mismatch_repair_CS. IPR014763. DNA_mismatch_repair_N. IPR015434. PMS2. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit. G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
PANTHER	PTHR10073. DNA_mis_repair. 1 hit. PTHR10073:SF9. PMS2. 1 hit.
Pfam	PF01119. DNA_mis_repair. 1 hit. PF02518. HATPase_c. 1 hit. [Graphical view]
SMART	SM00387. HATPase_c. 1 hit. [Graphical view]
SUPFAM	SSF55874. ATP_bd_ATPase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00585. Mutl. 1 hit.
PROSITE	PS00058. DNA_MISMATCH_REPAIR_1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	966802.

Entry information

Entry name

MLH2_YEAST

Accession

Primary (citable) accession number: Q07980
Secondary accession number(s): D6VY37

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2006
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program