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Protein

DNA mismatch repair protein MLH2

Gene

MLH2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in DNA mismatch repair (MMR), correcting insertion-deletion loops (IDLs) resulting from DNA replication, DNA damage or from recombination events between non-identical sequences during meiosis. Component of a MutL heterodimer with MLH1, which probably forms a ternary complex with the MutSbeta heterodimer that initially recognizes the DNA mismatches. This complex is thought to be responsible for directing the downsteam MMR events, including strand discrimination, excision, and resynthesis. Plays a role in the repair of heteroduplex sites present in meiotic recombination intermediates and is involved in maintaining the genetic stability of simple sequence repeats by correction of frameshift intermediates.3 Publications

GO - Molecular functioni

GO - Biological processi

  • mismatch repair Source: SGD
  • reciprocal meiotic recombination Source: SGD
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair

Enzyme and pathway databases

BioCyciYEAST:G3O-32194-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA mismatch repair protein MLH2
Alternative name(s):
MutL protein homolog 2
Gene namesi
Name:MLH2
Ordered Locus Names:YLR035C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR035C.
SGDiS000004025. MLH2.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 695695DNA mismatch repair protein MLH2PRO_0000245569Add
BLAST

Proteomic databases

MaxQBiQ07980.
PRIDEiQ07980.

Interactioni

Subunit structurei

Heterodimer of MLH1 and MLH2.

Binary interactionsi

WithEntry#Exp.IntActNotes
MLH1P389205EBI-33369,EBI-11003

Protein-protein interaction databases

BioGridi31309. 25 interactions.
DIPiDIP-2413N.
IntActiQ07980. 6 interactions.
MINTiMINT-502091.

Structurei

3D structure databases

ProteinModelPortaliQ07980.
SMRiQ07980. Positions 13-196.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000113599.
InParanoidiQ07980.
OrthoDBiEOG7FJH84.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR013507. DNA_mismatch_repair_C.
IPR014762. DNA_mismatch_repair_CS.
IPR002099. DNA_mismatch_repair_fam.
IPR003594. HATPase_C.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PfamiPF01119. DNA_mis_repair. 1 hit.
[Graphical view]
SMARTiSM01340. DNA_mis_repair. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR00585. mutl. 1 hit.
PROSITEiPS00058. DNA_MISMATCH_REPAIR_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q07980-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTIHQLSPES QWKIVSSSFI YGPVAAVREL LDNSIDSGAK KVFIDVDSTT
60 70 80 90 100
GGCEYISVKD DGSGVDIIDR PSMCLEYTTS KMSSLGDISI LTTLGFRGEA
110 120 130 140 150
LFLLSNLCNQ KGSMQVETKT ADDVIGEKWL VDSKGGITNG KRYKVSCPVG
160 170 180 190 200
TTVILRKLLG GLRARYLEIS SRPRKTFDEL IYLINHYSLI HRNIRFYFSL
210 220 230 240 250
VSLQKNGAIE RKQMQETLDP KISRARSLSL LARLKKPVPL NFIVEENFVI
260 270 280 290 300
DEKINLDLIL PRMVPESDVI NIKRRFKFLS VNERALSLNL ETGKTISKLL
310 320 330 340 350
SSIYRDFSLL DPMVWFINLN CDTKLLDVNI EPEKNDVMIK SFEVIFKKIE
360 370 380 390 400
NKLKLLLEER IGIETNMLGD KHVQPSINEK TSPALVIPTP DAENEISKGC
410 420 430 440 450
GAVSGKDKTD IPQKNSDLIV PTFYDEANLE NTTIVAATPS PTKFSEDKAL
460 470 480 490 500
DEQTQLTISS YRSSSSGSMA SEDSTNWRHN FQQELSENSE VAGAGSSTLP
510 520 530 540 550
SSLTYNYIET IPENEDLELS KDASISNPFM ITKIRNVNKK LSENLLEAKR
560 570 580 590 600
TSCGDANKRG MPNERQLSNT EKKELIKLQQ TYGKRNNTVD MTIPRNSKKK
610 620 630 640 650
VTDNYIKKAS CMHKTRPKLM HFSEYTNNYV YTLKNEKIVK HDSDNFAKET
660 670 680 690
LWLRSRDDAT SPSSSLLQAL RAHVKKPGHI EATTNEWCLF TPDSP
Length:695
Mass (Da):78,248
Last modified:November 1, 1996 - v1
Checksum:iDA5DA6AC86603A81
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73207 Genomic DNA. Translation: CAA97559.1.
BK006945 Genomic DNA. Translation: DAA09353.1.
PIRiS64862.
RefSeqiNP_013135.1. NM_001181922.1.

Genome annotation databases

EnsemblFungiiYLR035C; YLR035C; YLR035C.
GeneIDi850722.
KEGGisce:YLR035C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73207 Genomic DNA. Translation: CAA97559.1.
BK006945 Genomic DNA. Translation: DAA09353.1.
PIRiS64862.
RefSeqiNP_013135.1. NM_001181922.1.

3D structure databases

ProteinModelPortaliQ07980.
SMRiQ07980. Positions 13-196.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31309. 25 interactions.
DIPiDIP-2413N.
IntActiQ07980. 6 interactions.
MINTiMINT-502091.

Proteomic databases

MaxQBiQ07980.
PRIDEiQ07980.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR035C; YLR035C; YLR035C.
GeneIDi850722.
KEGGisce:YLR035C.

Organism-specific databases

EuPathDBiFungiDB:YLR035C.
SGDiS000004025. MLH2.

Phylogenomic databases

HOGENOMiHOG000113599.
InParanoidiQ07980.
OrthoDBiEOG7FJH84.

Enzyme and pathway databases

BioCyciYEAST:G3O-32194-MONOMER.

Miscellaneous databases

PROiQ07980.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR013507. DNA_mismatch_repair_C.
IPR014762. DNA_mismatch_repair_CS.
IPR002099. DNA_mismatch_repair_fam.
IPR003594. HATPase_C.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PfamiPF01119. DNA_mis_repair. 1 hit.
[Graphical view]
SMARTiSM01340. DNA_mis_repair. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR00585. mutl. 1 hit.
PROSITEiPS00058. DNA_MISMATCH_REPAIR_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Functional specificity of MutL homologs in yeast: evidence for three Mlh1-based heterocomplexes with distinct roles during meiosis in recombination and mismatch correction."
    Wang T.-F., Kleckner N., Hunter N.
    Proc. Natl. Acad. Sci. U.S.A. 96:13914-13919(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MLH1.
  4. "Discrete in vivo roles for the MutL homologs Mlh2p and Mlh3p in the removal of frameshift intermediates in budding yeast."
    Harfe B.D., Minesinger B.K., Jinks-Robertson S.
    Curr. Biol. 10:145-148(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "A role for the MutL homologue MLH2 in controlling heteroduplex formation and in regulating between two different crossover pathways in budding yeast."
    Abdullah M.F.F., Hoffmann E.R., Cotton V.E., Borts R.H.
    Cytogenet. Genome Res. 107:180-190(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiMLH2_YEAST
AccessioniPrimary (citable) accession number: Q07980
Secondary accession number(s): D6VY37
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 72 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.