Q07979 (RSC58_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chromatin structure-remodeling complex protein RSC58

Alternative name(s):
Remodel the structure of chromatin complex subunit 58

Gene names

Name:	RSC58
Ordered Locus Names:	YLR033W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	502 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the chromatin structure-remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. Ref.3 Ref.4 Ref.5 Ref.7 Ref.8 Ref.10
Subunit structure	Component of the two forms of the RSC complex composed of at least either RSC1 or RSC2, and ARP7, ARP9, LDB7, NPL6, RSC3, RSC30, RSC4, RSC58, RSC6, RSC8, RSC9, SFH1, STH1, HTL1 and probably RTT102. The complexes interact with histone and histone variant components of centromeric chromatin.
Subcellular location	Nucleus. Note: Localizes to centromeric and flanking chromatin. Association with these loci is dependent on STH1. Ref.10
Miscellaneous	Present with 4460 molecules/cell in log phase SD medium. Ref.11

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Molecular function	Chromatin regulator
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ATP-dependent chromatin remodeling Inferred from direct assay Ref.4. Source: UniProtKB G1/S transition of mitotic cell cycle Inferred from mutant phenotype. Source: SGD nucleosome disassembly Inferred from direct assay. Source: SGD regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription elongation from RNA polymerase II promoter Inferred from direct assay. Source: SGD
Cellular component	RSC complex Inferred from direct assay Ref.9. Source: UniProtKB
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RSC6	P25632	7	EBI-36549,EBI-21941
STH1	P32597	6	EBI-36549,EBI-18410

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 502	502	Chromatin structure-remodeling complex protein RSC58		PRO_0000097473

Sequences

	Sequence		Length	Mass (Da)
	Q07979 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 8F8D01184DAE1F6F	FASTA	502	57,794
	10 20 30 40 50 60 MTESVGGNKL VDFLVNVQSI LNAASVKCHV VDESFPAKFF EKNPDKIYES YCKFIKNRSN 70 80 90 100 110 120 SEGLIRNEDK LVLTTINKRF ENGEYEPIQG GFYKLYHDIK LVCTILIHFY PQGTRNYQLV 130 140 150 160 170 180 DKFYKFSSEL LLRECCRIGI ALTQTNNIKS RSGKLLSGNE MDEYDDDDAT ELDKIISYDF 190 200 210 220 230 240 IKISMNYTVP ISQTYQIRTK DMDLFSSIIS KSNLDKRPHE LPNTNFKINN VLPQTDIENE 250 260 270 280 290 300 APRLGFVGAN TSNIPDPTLP PTEMMTRFLH PNWYALPTTV WLKYGNYNSW APSFNENGTV 310 320 330 340 350 360 VDSTTRGLIW LERIGYMDLY EKNEKKVKQE ELLNTNEEGI NRKQNDENNK NVDGKSNGVQ 370 380 390 400 410 420 DDGGDNDNDA TIASANSEST ENKEQFIIKL QNLYNWTPSN YIGDDEIENF RNGTPDKLVS 430 440 450 460 470 480 DSLLKLKRLR KERILNKVLK PTTEERELYF KVKRILKEVI LAKKVSKVPI NNVRAFPVLQ 490 500 TNYNGSIPVV RAQPGRKRKH KK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"RSC, an essential, abundant chromatin-remodeling complex." Cairns B.R., Lorch Y., Li Y., Zhang M., Lacomis L., Erdjument-Bromage H., Tempst P., Du J., Laurent B.C., Kornberg R.D. Cell 87:1249-1260(1996) [PubMed: 8980231] [Abstract] Cited for: FUNCTION OF THE RSC COMPLEX.
[4]	"Histone octamer transfer by a chromatin-remodeling complex." Lorch Y., Zhang M., Kornberg R.D. Cell 96:389-392(1999) [PubMed: 10025404] [Abstract] Cited for: FUNCTION OF THE RSC COMPLEX.
[5]	"Transcriptional repression of the yeast CHA1 gene requires the chromatin-remodeling complex RSC." Moreira J.M.A., Holmberg S. EMBO J. 18:2836-2844(1999) [PubMed: 10329629] [Abstract] Cited for: FUNCTION OF THE RSC COMPLEX.
[6]	"Two functionally distinct forms of the RSC nucleosome-remodeling complex, containing essential AT hook, BAH, and bromodomains." Cairns B.R., Schlichter A., Erdjument-Bromage H., Tempst P., Kornberg R.D., Winston F. Mol. Cell 4:715-723(1999) [PubMed: 10619019] [Abstract] Cited for: COMPOSITION OF THE RSC COMPLEX.
[7]	"Chromatin remodeling by RSC involves ATP-dependent DNA translocation." Saha A., Wittmeyer J., Cairns B.R. Genes Dev. 16:2120-2134(2002) [PubMed: 12183366] [Abstract] Cited for: FUNCTION OF THE RSC COMPLEX.
[8]	"Yeast RSC function is required for organization of the cellular cytoskeleton via an alternative PKC1 pathway." Chai B., Hsu J.-M., Du J., Laurent B.C. Genetics 161:575-584(2002) [PubMed: 12072455] [Abstract] Cited for: FUNCTION OF THE RSC COMPLEX.
[9]	"Proteomics of the eukaryotic transcription machinery: identification of proteins associated with components of yeast TFIID by multidimensional mass spectrometry." Sanders S.L., Jennings J., Canutescu A., Link A.J., Weil P.A. Mol. Cell. Biol. 22:4723-4738(2002) [PubMed: 12052880] [Abstract] Cited for: IDENTIFICATION IN THE RSC COMPLEX.
[10]	"The yeast RSC chromatin-remodeling complex is required for kinetochore function in chromosome segregation." Hsu J.-M., Huang J., Meluh P.B., Laurent B.C. Mol. Cell. Biol. 23:3202-3215(2003) [PubMed: 12697820] [Abstract] Cited for: FUNCTION OF THE RSC COMPLEX, SUBCELLULAR LOCATION, INTERACTION OF THE RSC COMPLEX WITH HISTONES.
[11]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z73205 Genomic DNA. Translation: CAA97557.1. BK006945 Genomic DNA. Translation: DAA09351.1.
PIR	S64860.
RefSeq	NP_013133.1. NM_001181920.1.
3D structure databases
ProteinModelPortal	Q07979.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6656N.
IntAct	Q07979. 106 interactions.
MINT	MINT-630532.
STRING	Q07979.
Proteomic databases
PeptideAtlas	Q07979.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR033W; YLR033W; YLR033W.
GeneID	850720.
KEGG	sce:YLR033W.
NMPDR	fig|4932.3.peg.4124.
Organism-specific databases
CYGD	YLR033w.
SGD	S000004023. RSC58.
Phylogenomic databases
eggNOG	fuNOG08189.
HOGENOM	HBG512130.
OMA	PPTEMMT.
OrthoDB	EOG4FN7SH.
Gene expression databases
ArrayExpress	Q07979.
Genevestigator	Q07979.
GermOnline	YLR033W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001487. Bromodomain. [Graphical view]
KO	K11766.
Pfam	PF00439. Bromodomain. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	966796.

Entry information

Entry name

RSC58_YEAST

Accession

Primary (citable) accession number: Q07979
Secondary accession number(s): D6VY35

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program