Q07977 (SIA8B_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-2,8-sialyltransferase 8B
EC=2.4.99.-

Alternative name(s):
Sialyltransferase 8B
Short name=SIAT8-B
Sialyltransferase St8Sia II
Short name=ST8SiaII
Sialyltransferase X
Short name=STX

Gene names

Name:	St8sia2
Synonyms:	Siat8b, Stx

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May transfer sialic acid through alpha-2,8-linkages to the alpha-2,3-linked and alpha-2,6-linked sialic acid of N-linked oligosaccharides of glycoproteins and may be involved in PSA (polysialic acid) expression.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Potential.
Tissue specificity	Expressed only in newborn brain.
Developmental stage	Newborn.
Sequence similarities	Belongs to the glycosyltransferase 29 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	N-glycan processing Inferred from electronic annotation. Source: Compara ganglioside biosynthetic process Inferred from electronic annotation. Source: Compara
Cellular_component	integral to Golgi membrane Inferred from electronic annotation. Source: InterPro
Molecular_function	alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity Inferred from electronic annotation. Source: Compara sialyltransferase activity Traceable author statement Ref.1. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 375	375	Alpha-2,8-sialyltransferase 8B		PRO_0000149288
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 23	17	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	24 – 375	352	Lumenal Potential
Amino acid modifications
	Glycosylation	60	1	N-linked (GlcNAc...) Potential
	Glycosylation	72	1	N-linked (GlcNAc...) Potential
	Glycosylation	89	1	N-linked (GlcNAc...) Potential
	Glycosylation	134	1	N-linked (GlcNAc...) Potential
	Glycosylation	219	1	N-linked (GlcNAc...) Potential
	Glycosylation	234	1	N-linked (GlcNAc...) Potential
	Disulfide bond	157 ↔ 307		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q07977 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: F7B3AC1E1E503675	FASTA	375	42,400
	10 20 30 40 50 60 MQLQFRSWML AALTLLVVFL IFADISEIEE EIGNSGGRGT IRSAVNSLHS KSNRAEVVIN 70 80 90 100 110 120 GSSLPAVADR SNESLKHSIQ PASSKWRHNQ TLSLRIRKQI LKFLDAEKDI SVLKGTLKPG 130 140 150 160 170 180 DIIHYIFDRD STMNVSQNLY ELLPRTSPLK NKHFQTCAIV GNSGVLLNSG CGQEIDTHSF 190 200 210 220 230 240 VIRCNLAPVQ EYARDVGLKT DLVTMNPSVI QRAFEDLVNA TWREKLLQRL HGLNGSILWI 250 260 270 280 290 300 PAFMARGGKE RVEWVNALIL KHHVNVRTAY PSLRLLHAVR GYWLTNKVHI KRPTTGLLMY 310 320 330 340 350 360 TLATRFCNQI YLYGFWPFPL DQNQNPVKYH YYDSLKYGYT SQASPHTMPL EFKALKSLHE 370 QGALKLTVGQ CDGAT

References

[1]	"Polymerase chain reaction cloning of a developmentally regulated member of the sialyltransferase gene family." Livingston B.D., Paulson J.C. J. Biol. Chem. 268:11504-11507(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Brain.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L13445 mRNA. Translation: AAA42147.1.
IPI	IPI00201899.
PIR	A46727.
RefSeq	NP_476497.1. NM_057156.1.
UniGene	Rn.44391.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000016427.
Protein family/group databases
CAZy	GT29. Glycosyltransferase Family 29.
PTM databases
PhosphoSite	Q07977.
Proteomic databases
PaxDb	Q07977.
PRIDE	Q07977.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	117523.
KEGG	rno:117523.
UCSC	RGD:621843. rat.
Organism-specific databases
CTD	8128.
RGD	621843. St8sia2.
Phylogenomic databases
eggNOG	NOG324948.
HOGENOM	HOG000232070.
HOVERGEN	HBG056466.
InParanoid	Q07977.
KO	K06612.
OrthoDB	EOG4FTW0Z.
Enzyme and pathway databases
UniPathway	UPA00378.
Gene expression databases
ArrayExpress	Q07977.
Genevestigator	Q07977.
GermOnline	ENSRNOG00000012031. Rattus norvegicus.
Family and domain databases
InterPro	IPR001675. Glyco_trans_29. IPR012163. Sialyl_trans. [Graphical view]
Pfam	PF00777. Glyco_transf_29. 1 hit. [Graphical view]
PIRSF	PIRSF005557. Sialyl_trans. 1 hit.
ProtoNet	Search...
Other
NextBio	620309.

Entry information

Entry name

SIA8B_RAT

Accession

Primary (citable) accession number: Q07977
Secondary accession number(s): Q64688

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program