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Protein

Kinesin-1

Gene

ATK1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Kinesin that supports microtubule movement in an ATP-dependent manner and has a minus-end directed polarity. Plays a crucial role in spindle morphogenesis in male Arabidopsis meiosis. In mitosis, is required for normal microtubule accumulation at the spindle poles during prophase and may play a role in spindle assembly during prometaphase.3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi516 – 5238ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent microtubule motor activity, minus-end-directed Source: TAIR

GO - Biological processi

  • anastral spindle assembly involved in male meiosis Source: TAIR
  • cell division Source: UniProtKB-KW
  • microtubule-based movement Source: GO_Central
  • mitotic nuclear division Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Biological processi

Cell cycle, Cell division, Meiosis, Mitosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:GQT-1964-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Kinesin-1
Alternative name(s):
Kinesin-like protein A
Gene namesi
Name:ATK1
Synonyms:KATA
Ordered Locus Names:At4g21270
ORF Names:F7J7.210
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G21270.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: TAIR
  • microtubule Source: UniProtKB-KW
  • minus-end kinesin complex Source: TAIR
  • nucleus Source: TAIR
  • phragmoplast Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Disruption phenotypei

Plants show defects in male meiosis, producing an abnormal number of microspores of variable sizes. Dividing cells of mutant plants lack spindle bipolarity in metaphase of mitosis.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 793793Kinesin-1PRO_0000125380Add
BLAST

Proteomic databases

PaxDbiQ07970.
PRIDEiQ07970.

Expressioni

Gene expression databases

GenevisibleiQ07970. AT.

Interactioni

Protein-protein interaction databases

BioGridi13167. 2 interactions.
MINTiMINT-8061432.
STRINGi3702.AT4G21270.1.

Structurei

3D structure databases

ProteinModelPortaliQ07970.
SMRiQ07970. Positions 431-769.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini431 – 772342Kinesin motorPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 6969GlobularAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili70 – 428359Add
BLAST

Domaini

Composed of three structural domains; a small globular N-terminal, a central alpha-helical coiled coil and a large globular C-terminal which is responsible for the motor activity (it hydrolyzes ATP and binds microtubules).

Sequence similaritiesi

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. NCD subfamily.PROSITE-ProRule annotation
Contains 1 kinesin motor domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0239. Eukaryota.
COG5059. LUCA.
HOGENOMiHOG000116164.
InParanoidiQ07970.
KOiK10405.
OMAiQADETHV.
PhylomeDBiQ07970.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 3 hits.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q07970-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASRNQNRPP RSPNAKKEGL GGISFDKRRK VETQGGTGRR QAFSAVNKQD
60 70 80 90 100
VTMNSDVGSI EECGKVDFTK DEILALLSER AKAGKFDTKA KIEQMTDIIK
110 120 130 140 150
RLKVCVKWFQ QADETHVQEK ENLKVSLESS EQKYNHKELE ARTKEEELQA
160 170 180 190 200
TISKLEENVV SLHEKLAKEE SSTQDAIECH RREKEARVAA EKVQASLGEE
210 220 230 240 250
LDKVKEEKMA AKQKVTSLED MYKRLQEYNT SLQQYNSKLQ TDLETVRAAL
260 270 280 290 300
TRAEKEKSSI LENLSTLRGH SKSLQDQLSS SRVLQDDAIK QKDSLLSEVT
310 320 330 340 350
NLRNELQQVR DDRDRQVVQS QKLSEEIRKY QENVGKSSQE LDILTAKSGS
360 370 380 390 400
LEETCSLQKE RLNMLEQQLA IANERQKMAD ASVSLTRTEF EEQKHLLCEL
410 420 430 440 450
QDRLADMEHQ LCEGELLRKK LHNTILELKG NIRVFCRVRP LLPDDGGRHE
460 470 480 490 500
ATVIAYPTST EAQGRGVDLV QSGNKHPFTF DKVFNHEASQ EEVFFEISQL
510 520 530 540 550
VQSALDGYKV CIFAYGQTGS GKTYTMMGRP EAPDQKGLIP RSLEQIFQAS
560 570 580 590 600
QSLGAQGWKY KMQVSMLEIY NETIRDLLST NRTTSMDLVR ADSGTSGKQY
610 620 630 640 650
TITHDVNGHT HVSDLTIFDV CSVGKISSLL QQAAQSRSVG KTQMNEQSSR
660 670 680 690 700
SHFVFTMRIS GVNESTEQQV QGVLNLIDLA GSERLSKSGA TGDRLKETQA
710 720 730 740 750
INKSLSALSD VIFALAKKED HVPFRNSKLT YLLQPCLGGD SKTLMFVNIS
760 770 780 790
PDPTSAGESL CSLRFAARVN ACEIGIPRRQ TSTKLLDSRL SYG
Length:793
Mass (Da):89,047
Last modified:November 1, 1995 - v1
Checksum:i85A195206D825C4E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D11371 mRNA. Translation: BAA01972.1.
AL021960 Genomic DNA. Translation: CAA17546.1.
AL161554 Genomic DNA. Translation: CAB79127.1.
CP002687 Genomic DNA. Translation: AEE84434.1.
PIRiS34830.
RefSeqiNP_193859.1. NM_118246.1.
UniGeneiAt.274.

Genome annotation databases

EnsemblPlantsiAT4G21270.1; AT4G21270.1; AT4G21270.
GeneIDi827876.
GrameneiAT4G21270.1; AT4G21270.1; AT4G21270.
KEGGiath:AT4G21270.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D11371 mRNA. Translation: BAA01972.1.
AL021960 Genomic DNA. Translation: CAA17546.1.
AL161554 Genomic DNA. Translation: CAB79127.1.
CP002687 Genomic DNA. Translation: AEE84434.1.
PIRiS34830.
RefSeqiNP_193859.1. NM_118246.1.
UniGeneiAt.274.

3D structure databases

ProteinModelPortaliQ07970.
SMRiQ07970. Positions 431-769.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi13167. 2 interactions.
MINTiMINT-8061432.
STRINGi3702.AT4G21270.1.

Proteomic databases

PaxDbiQ07970.
PRIDEiQ07970.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G21270.1; AT4G21270.1; AT4G21270.
GeneIDi827876.
GrameneiAT4G21270.1; AT4G21270.1; AT4G21270.
KEGGiath:AT4G21270.

Organism-specific databases

TAIRiAT4G21270.

Phylogenomic databases

eggNOGiKOG0239. Eukaryota.
COG5059. LUCA.
HOGENOMiHOG000116164.
InParanoidiQ07970.
KOiK10405.
OMAiQADETHV.
PhylomeDBiQ07970.

Enzyme and pathway databases

BioCyciARA:GQT-1964-MONOMER.

Miscellaneous databases

PROiQ07970.

Gene expression databases

GenevisibleiQ07970. AT.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 3 hits.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a gene family (kat) encoding kinesin-like proteins in Arabidopsis thaliana and the characterization of secondary structure of KatA."
    Mitsui H., Yamaguchi-Shinozaki K., Shinozaki K., Nishikawa K., Takahashi H.
    Mol. Gen. Genet. 238:362-368(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Arabidopsis thaliana protein, ATK1, is a minus-end directed kinesin that exhibits non-processive movement."
    Marcus A.I., Ambrose J.C., Blickley L., Hancock W.O., Cyr R.J.
    Cell Motil. Cytoskeleton 52:144-150(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "The Arabidopsis ATK1 gene is required for spindle morphogenesis in male meiosis."
    Chen C., Marcus A., Li W., Hu Y., Calzada J.-P., Grossniklaus U., Cyr R.J., Ma H.
    Development 129:2401-2409(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  6. "A kinesin mutant with an atypical bipolar spindle undergoes normal mitosis."
    Marcus A.I., Li W., Ma H., Cyr R.J.
    Mol. Biol. Cell 14:1717-1726(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiATK1_ARATH
AccessioniPrimary (citable) accession number: Q07970
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: February 17, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.