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Protein

Kinesin-like protein KIN-14C

Gene

KIN14C

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Kinesin that supports microtubule movement in an ATP-dependent manner and has a minus-end directed polarity. Plays a crucial role in spindle morphogenesis in male meiosis. In mitosis, is required for normal microtubule accumulation at the spindle poles during prophase and may play a role in spindle assembly during prometaphase.4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi516 – 523ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent microtubule motor activity, minus-end-directed Source: TAIR

GO - Biological processi

  • anastral spindle assembly involved in male meiosis Source: TAIR
  • cell division Source: UniProtKB-KW
  • microtubule-based movement Source: GO_Central
  • mitotic nuclear division Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Biological processi

Cell cycle, Cell division, Meiosis, Mitosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:GQT-1964-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Kinesin-like protein KIN-14CCurated
Alternative name(s):
AtKIN14a1 Publication
Kinesin-like protein KatA2 Publications
Gene namesi
Name:KIN14CCurated
Synonyms:ATK11 Publication, KATA1 PublicationImported
Ordered Locus Names:At4g21270Imported
ORF Names:F7J7.210Imported, T6K22.10Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G21270.

Subcellular locationi

  • Cytoplasmcytoskeletonspindle 1 Publication
  • Cytoplasmcytoskeletonphragmoplast 1 Publication
  • Chromosomecentromerekinetochore 1 Publication
  • Cytoplasmcytoskeleton 1 Publication

  • Note: Colocalizes with microtubules during preprophase and cytokinesis. In M-phase, locates to the preprophase band. Present mainly in the midzone at metaphase and later during anaphase. Associated with the ends of the kinetochore fibers near the kinetochores. Detected on the phragmoplast by late cytokinesis.1 Publication

GO - Cellular componenti

  • condensed chromosome kinetochore Source: UniProtKB-SubCell
  • cytoplasm Source: TAIR
  • microtubule Source: UniProtKB-KW
  • minus-end kinesin complex Source: TAIR
  • nucleus Source: TAIR
  • phragmoplast Source: TAIR
  • spindle Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Microtubule

Pathology & Biotechi

Disruption phenotypei

Plants show defects in male meiosis, producing an abnormal number of microspores of variable sizes. Dividing cells of mutant plants lack spindle bipolarity in metaphase of mitosis. Kin14c and kin14d double mutant is gametophytically lethal (PubMed:18088313).3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001253801 – 793Kinesin-like protein KIN-14CAdd BLAST793

Proteomic databases

PaxDbiQ07970.
PRIDEiQ07970.

PTM databases

iPTMnetiQ07970.

Expressioni

Gene expression databases

GenevisibleiQ07970. AT.

Interactioni

Protein-protein interaction databases

BioGridi13167. 2 interactors.
MINTiMINT-8061432.
STRINGi3702.AT4G21270.1.

Structurei

3D structure databases

ProteinModelPortaliQ07970.
SMRiQ07970.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini431 – 772Kinesin motorPROSITE-ProRule annotationAdd BLAST342

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 69GlobularAdd BLAST69

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili120 – 375Sequence analysisAdd BLAST256

Domaini

Composed of three structural domains; a small globular N-terminal, a central alpha-helical coiled coil and a large globular C-terminal which is responsible for the motor activity (it hydrolyzes ATP and binds microtubules).

Sequence similaritiesi

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-14 subfamily.1 Publication
Contains 1 kinesin motor domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0239. Eukaryota.
COG5059. LUCA.
HOGENOMiHOG000116164.
InParanoidiQ07970.
KOiK10405.
OMAiQADETHV.
OrthoDBiEOG0936041R.
PhylomeDBiQ07970.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 3 hits.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q07970-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASRNQNRPP RSPNAKKEGL GGISFDKRRK VETQGGTGRR QAFSAVNKQD
60 70 80 90 100
VTMNSDVGSI EECGKVDFTK DEILALLSER AKAGKFDTKA KIEQMTDIIK
110 120 130 140 150
RLKVCVKWFQ QADETHVQEK ENLKVSLESS EQKYNHKELE ARTKEEELQA
160 170 180 190 200
TISKLEENVV SLHEKLAKEE SSTQDAIECH RREKEARVAA EKVQASLGEE
210 220 230 240 250
LDKVKEEKMA AKQKVTSLED MYKRLQEYNT SLQQYNSKLQ TDLETVRAAL
260 270 280 290 300
TRAEKEKSSI LENLSTLRGH SKSLQDQLSS SRVLQDDAIK QKDSLLSEVT
310 320 330 340 350
NLRNELQQVR DDRDRQVVQS QKLSEEIRKY QENVGKSSQE LDILTAKSGS
360 370 380 390 400
LEETCSLQKE RLNMLEQQLA IANERQKMAD ASVSLTRTEF EEQKHLLCEL
410 420 430 440 450
QDRLADMEHQ LCEGELLRKK LHNTILELKG NIRVFCRVRP LLPDDGGRHE
460 470 480 490 500
ATVIAYPTST EAQGRGVDLV QSGNKHPFTF DKVFNHEASQ EEVFFEISQL
510 520 530 540 550
VQSALDGYKV CIFAYGQTGS GKTYTMMGRP EAPDQKGLIP RSLEQIFQAS
560 570 580 590 600
QSLGAQGWKY KMQVSMLEIY NETIRDLLST NRTTSMDLVR ADSGTSGKQY
610 620 630 640 650
TITHDVNGHT HVSDLTIFDV CSVGKISSLL QQAAQSRSVG KTQMNEQSSR
660 670 680 690 700
SHFVFTMRIS GVNESTEQQV QGVLNLIDLA GSERLSKSGA TGDRLKETQA
710 720 730 740 750
INKSLSALSD VIFALAKKED HVPFRNSKLT YLLQPCLGGD SKTLMFVNIS
760 770 780 790
PDPTSAGESL CSLRFAARVN ACEIGIPRRQ TSTKLLDSRL SYG
Length:793
Mass (Da):89,047
Last modified:November 1, 1995 - v1
Checksum:i85A195206D825C4E
GO

Sequence cautioni

The sequence CAA20193 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D11371 mRNA. Translation: BAA01972.1.
AL021960 Genomic DNA. Translation: CAA17546.1.
AL031187 Genomic DNA. Translation: CAA20193.1. Sequence problems.
AL161554 Genomic DNA. Translation: CAB79127.1.
CP002687 Genomic DNA. Translation: AEE84434.1.
PIRiS34830.
RefSeqiNP_193859.1. NM_118246.2.
UniGeneiAt.274.

Genome annotation databases

EnsemblPlantsiAT4G21270.1; AT4G21270.1; AT4G21270.
GeneIDi827876.
GrameneiAT4G21270.1; AT4G21270.1; AT4G21270.
KEGGiath:AT4G21270.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D11371 mRNA. Translation: BAA01972.1.
AL021960 Genomic DNA. Translation: CAA17546.1.
AL031187 Genomic DNA. Translation: CAA20193.1. Sequence problems.
AL161554 Genomic DNA. Translation: CAB79127.1.
CP002687 Genomic DNA. Translation: AEE84434.1.
PIRiS34830.
RefSeqiNP_193859.1. NM_118246.2.
UniGeneiAt.274.

3D structure databases

ProteinModelPortaliQ07970.
SMRiQ07970.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi13167. 2 interactors.
MINTiMINT-8061432.
STRINGi3702.AT4G21270.1.

PTM databases

iPTMnetiQ07970.

Proteomic databases

PaxDbiQ07970.
PRIDEiQ07970.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G21270.1; AT4G21270.1; AT4G21270.
GeneIDi827876.
GrameneiAT4G21270.1; AT4G21270.1; AT4G21270.
KEGGiath:AT4G21270.

Organism-specific databases

TAIRiAT4G21270.

Phylogenomic databases

eggNOGiKOG0239. Eukaryota.
COG5059. LUCA.
HOGENOMiHOG000116164.
InParanoidiQ07970.
KOiK10405.
OMAiQADETHV.
OrthoDBiEOG0936041R.
PhylomeDBiQ07970.

Enzyme and pathway databases

BioCyciARA:GQT-1964-MONOMER.

Miscellaneous databases

PROiQ07970.

Gene expression databases

GenevisibleiQ07970. AT.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 3 hits.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKN14C_ARATH
AccessioniPrimary (citable) accession number: Q07970
Secondary accession number(s): Q7FKK6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.