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Protein

E3 ubiquitin-protein ligase UBR2

Gene

UBR2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase which probably functions outside the N-end rule pathway, since it lacks the residues essential for the degradation of N-end rule substrates. Mediates RPN4 ubiquitination and subsequent degradation.2 Publications

Miscellaneous

Present with 49 molecules/cell in log phase SD medium.1 Publication
Deletion produces no detectable phenotype.

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri96 – 172UBR-typePROSITE-ProRule annotationAdd BLAST77
Zinc fingeri1241 – 1362RING-type; atypicalAdd BLAST122

GO - Molecular functioni

GO - Biological processi

  • cytoplasm protein quality control by the ubiquitin-proteasome system Source: SGD
  • protein polyubiquitination Source: SGD
  • ubiquitin-dependent protein catabolic process Source: SGD
  • ubiquitin-dependent protein catabolic process via the N-end rule pathway Source: GO_Central

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32185-MONOMER
ReactomeiR-SCE-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase UBR2 (EC:2.3.2.27)
Alternative name(s):
RING-type E3 ubiquitin transferase UBR2
Ubiquitin-protein ligase E3 component N-recognin-1 homolog
Gene namesi
Name:UBR2
Ordered Locus Names:YLR024C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR024C
SGDiS000004014 UBR2

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000561421 – 1872E3 ubiquitin-protein ligase UBR2Add BLAST1872

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki709Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Modified residuei1218PhosphoserineCombined sources1
Modified residuei1222PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ07963
PaxDbiQ07963
PRIDEiQ07963

PTM databases

iPTMnetiQ07963

Interactioni

Subunit structurei

Interacts with MUB1, RPN4 and UBC2.3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi31298, 432 interactors
DIPiDIP-6593N
IntActiQ07963, 14 interactors
MINTiQ07963
STRINGi4932.YLR024C

Structurei

3D structure databases

ProteinModelPortaliQ07963
SMRiQ07963
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1134 – 1240Interaction with UBC2Add BLAST107

Domaini

The RING-H2 zinc finger is an atypical RING finger with a His ligand in place of the fourth Cys of the classical motif.

Sequence similaritiesi

Belongs to the UBR1 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri96 – 172UBR-typePROSITE-ProRule annotationAdd BLAST77
Zinc fingeri1241 – 1362RING-type; atypicalAdd BLAST122

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00530000063055
HOGENOMiHOG000141750
InParanoidiQ07963
OMAiSMREFTY
OrthoDBiEOG092C01SD

Family and domain databases

InterProiView protein in InterPro
IPR003126 Znf_UBR
PfamiView protein in Pfam
PF02207 zf-UBR, 1 hit
SMARTiView protein in SMART
SM00396 ZnF_UBR1, 1 hit
PROSITEiView protein in PROSITE
PS51157 ZF_UBR, 1 hit

Sequencei

Sequence statusi: Complete.

Q07963-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDSDLSITN IRDFLTELPK LAKCEYSETT SYLLWKTLNL RLKHSDNDIN
60 70 80 90 100
WRSLVSILNS EAWENEKYRD ILNGRKWRTL EFENDHHSVG NMHIGTACTR
110 120 130 140 150
LCFPSETIYY CFTCSTNPLY EICELCFDKE KHVNHSYVAK VVMRPEGRIC
160 170 180 190 200
HCGDPFAFND PSDAFKCKNE LNNIPISNDN SNVTDDENVI SLLNYVLDFL
210 220 230 240 250
IDVTVSYKEE AEAHSSERKA SSLMHPNQNS ITDDIMEKHE CEPLVNDENF
260 270 280 290 300
VFFDNNWSNT RKEAHMEWAI QIEEEECNVH YMDLASTITR ILNTPVEYAI
310 320 330 340 350
SITKALEDSH DVVTVLQSEN FFEIDQIAKE FQKENIVVHV RKADDIFKRK
360 370 380 390 400
LTDDLTDWLY SLCFKAATSL QNKYALRISM LDVWYSHFSK MRVSPTNTNP
410 420 430 440 450
DFSKINLLGG FLISNEDSDE SWFKPWSLEN IEDERISKIL TNYNERLIRA
460 470 480 490 500
HSPNTVSHFY NFYGSRFQYI IINSINILSK KSKFKMLKIM ASLFSLRDES
510 520 530 540 550
RKFLAAQYID VYLSVLYDAV ASDAKECQVT LMSILGQYTF QDPSIANMTI
560 570 580 590 600
SSGFIERTIR FAFTLMAFNP EDLMSYLPIS LYNGFKLPTE TIRNRRTIIC
610 620 630 640 650
FKDLCTIMSA NTVPEELLSN EAIFNAIIES FSEFSNVLPL KRETKEHVEV
660 670 680 690 700
ENFDFSAFYF FFSSILIMTD GYTRSISLVK DAAFRKQIVL KLLDVAQTRE
710 720 730 740 750
FESLTNSRKA ISPDNASTNE NDSNKATLST VRETICNYVA ETINFQVGVN
760 770 780 790 800
TQYFFNPMSY LFKFVIQWSQ CGRYEPIPAS LTNYINLFEV FQDKQKALYI
810 820 830 840 850
SESALSTLVL IGQINVGFWV RNGTPITHQA RMYTKYSMRE FTYISDIFNV
860 870 880 890 900
QFSMAMCNPD ELMVTYLSRW GLKHWANGVP MYDYPDTETT VAVVNECILL
910 920 930 940 950
LIQLLTEVRS LVMKSSKEGF ERTFKSEIIH ALCFDTCSYA QIVNCIPEHI
960 970 980 990 1000
TKHPSFDIYL EKYANYTSPV SLTDNGIFVL KEKYKDEIDP YYIGLSSSRR
1010 1020 1030 1040 1050
YDVEKNIRLN MANLKKMKYE DTFVPAKKVK DLLKNTLFSG LYSISSVNTF
1060 1070 1080 1090 1100
GLFLKNTLDH IIKYDYDNLL PRVVHLIHLC VVNNLNEFMG ILWHEYAIVD
1110 1120 1130 1140 1150
TEFCHYHSIG SILYYCLLKD NFSESHGKIR EIFRYLMETA PHVNVNSYLR
1160 1170 1180 1190 1200
EQTTSYTPGI LWPTKEDKSH KDKEFERKKH LARLRKKKLM KKLAQQQMKF
1210 1220 1230 1240 1250
MENNSVDTSD ISTPRTTSPS LSPTRINAEN SSNTINSCCD DDCVFCKMPK
1260 1270 1280 1290 1300
DDDVFVYFSY QERNICDHGI DFTNPTDVNR INSLFSGKQT KDSAIQENPQ
1310 1320 1330 1340 1350
DDDGTRLKFT SCEPVLRACG HGSHTKCLSG HMKSIRGIQN QTTKNIPLSY
1360 1370 1380 1390 1400
GSGLIYCPVC NSLSNSFLPK TNDIDKRTSS QFFMCIEKRS EAEENLDPMS
1410 1420 1430 1440 1450
SICIKAAMIL GDLQGKKVTT IEDAYKVVNS VFINTISNTE LRLRSHKKEG
1460 1470 1480 1490 1500
KIVNMERISS QCILTLHLVC ELKSFIYKKF VNSKTFSSEI SRKIWNWNEF
1510 1520 1530 1540 1550
LIKGNNVNLL LYMSQNFDNI DGGKTPQPPN LCIYEMFKRR FHQLLLLLAR
1560 1570 1580 1590 1600
DMMRVNFYKD CRNKIKISSN GSEEPSTSFS YLFNTFKKYV DLFKPDDVRF
1610 1620 1630 1640 1650
DFTSLEKIKD FICSLLLESL SIFCRRTFLL FNIQYDDDGD GDNNNNRSNN
1660 1670 1680 1690 1700
FMDVKQREIE LIFRYFKLPN LTHFLKDFFY NELTQNIERY NDGNDNLRIQ
1710 1720 1730 1740 1750
QVIYDMVQNI NTRAYPSPEH IQLIELPLNL SKFSLDNDEI SNKCDKYEIA
1760 1770 1780 1790 1800
VCLLCGQKCH IQKSIALQGY LQGECTDHMR NGCEITSAYG VFLMTGTNAI
1810 1820 1830 1840 1850
YLSYGKRGTF YAAPYLSKYG ETNEDYKFGT PVYLNRARYA NLANEIVFGN
1860 1870
MIPHIVFRLT DGSADLGGWE TM
Length:1,872
Mass (Da):216,778
Last modified:November 1, 1996 - v1
Checksum:i1F60CC41EB88752F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73196 Genomic DNA Translation: CAA97547.1
BK006945 Genomic DNA Translation: DAA09342.1
PIRiS64851
RefSeqiNP_013124.1, NM_001181911.2

Genome annotation databases

EnsemblFungiiYLR024C; YLR024C; YLR024C
GeneIDi850711
KEGGisce:YLR024C

Similar proteinsi

Entry informationi

Entry nameiUBR2_YEAST
AccessioniPrimary (citable) accession number: Q07963
Secondary accession number(s): D6VY26
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: November 1, 1996
Last modified: April 25, 2018
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health