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Protein

E3 ubiquitin-protein ligase UBR2

Gene

UBR2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase which probably functions outside the N-end rule pathway, since it lacks the residues essential for the degradation of N-end rule substrates. Mediates RPN4 ubiquitination and subsequent degradation.2 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri96 – 17277UBR-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1241 – 1362122RING-type; atypicalAdd
BLAST

GO - Molecular functioni

  • ligase activity Source: UniProtKB-KW
  • ubiquitin-protein transferase activity Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • cytoplasm-associated proteasomal ubiquitin-dependent protein catabolic process Source: SGD
  • protein polyubiquitination Source: SGD
  • protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32185-MONOMER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase UBR2 (EC:6.3.2.-)
Alternative name(s):
Ubiquitin-protein ligase E3 component N-recognin-1 homolog
Gene namesi
Name:UBR2
Ordered Locus Names:YLR024C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR024C.
SGDiS000004014. UBR2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • MUB1-RAD6-UBR2 ubiquitin ligase complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 18721872E3 ubiquitin-protein ligase UBR2PRO_0000056142Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki709 – 709Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Modified residuei1218 – 12181PhosphoserineCombined sources
Modified residuei1222 – 12221PhosphoserineCombined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ07963.
PeptideAtlasiQ07963.

PTM databases

iPTMnetiQ07963.

Interactioni

Subunit structurei

Interacts with MUB1, RPN4 and UBC2.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MUB1Q031622EBI-34338,EBI-28207
RAD6P061046EBI-34338,EBI-19722
RPN4Q034652EBI-34338,EBI-15931

Protein-protein interaction databases

BioGridi31298. 161 interactions.
DIPiDIP-6593N.
IntActiQ07963. 4 interactions.
MINTiMINT-694878.

Structurei

3D structure databases

ProteinModelPortaliQ07963.
SMRiQ07963. Positions 93-170.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1134 – 1240107Interaction with UBC2Add
BLAST

Domaini

The RING-H2 zinc finger is an atypical RING finger with a His ligand in place of the fourth Cys of the classical motif.

Sequence similaritiesi

Belongs to the UBR1 family.Curated
Contains 1 RING-type zinc finger.Curated
Contains 1 UBR-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri96 – 17277UBR-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1241 – 1362122RING-type; atypicalAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00530000063055.
HOGENOMiHOG000141750.
InParanoidiQ07963.
OMAiKYSMREF.
OrthoDBiEOG7GBG57.

Family and domain databases

InterProiIPR003126. Znf_UBR.
[Graphical view]
PfamiPF02207. zf-UBR. 1 hit.
[Graphical view]
SMARTiSM00396. ZnF_UBR1. 1 hit.
[Graphical view]
PROSITEiPS51157. ZF_UBR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q07963-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDSDLSITN IRDFLTELPK LAKCEYSETT SYLLWKTLNL RLKHSDNDIN
60 70 80 90 100
WRSLVSILNS EAWENEKYRD ILNGRKWRTL EFENDHHSVG NMHIGTACTR
110 120 130 140 150
LCFPSETIYY CFTCSTNPLY EICELCFDKE KHVNHSYVAK VVMRPEGRIC
160 170 180 190 200
HCGDPFAFND PSDAFKCKNE LNNIPISNDN SNVTDDENVI SLLNYVLDFL
210 220 230 240 250
IDVTVSYKEE AEAHSSERKA SSLMHPNQNS ITDDIMEKHE CEPLVNDENF
260 270 280 290 300
VFFDNNWSNT RKEAHMEWAI QIEEEECNVH YMDLASTITR ILNTPVEYAI
310 320 330 340 350
SITKALEDSH DVVTVLQSEN FFEIDQIAKE FQKENIVVHV RKADDIFKRK
360 370 380 390 400
LTDDLTDWLY SLCFKAATSL QNKYALRISM LDVWYSHFSK MRVSPTNTNP
410 420 430 440 450
DFSKINLLGG FLISNEDSDE SWFKPWSLEN IEDERISKIL TNYNERLIRA
460 470 480 490 500
HSPNTVSHFY NFYGSRFQYI IINSINILSK KSKFKMLKIM ASLFSLRDES
510 520 530 540 550
RKFLAAQYID VYLSVLYDAV ASDAKECQVT LMSILGQYTF QDPSIANMTI
560 570 580 590 600
SSGFIERTIR FAFTLMAFNP EDLMSYLPIS LYNGFKLPTE TIRNRRTIIC
610 620 630 640 650
FKDLCTIMSA NTVPEELLSN EAIFNAIIES FSEFSNVLPL KRETKEHVEV
660 670 680 690 700
ENFDFSAFYF FFSSILIMTD GYTRSISLVK DAAFRKQIVL KLLDVAQTRE
710 720 730 740 750
FESLTNSRKA ISPDNASTNE NDSNKATLST VRETICNYVA ETINFQVGVN
760 770 780 790 800
TQYFFNPMSY LFKFVIQWSQ CGRYEPIPAS LTNYINLFEV FQDKQKALYI
810 820 830 840 850
SESALSTLVL IGQINVGFWV RNGTPITHQA RMYTKYSMRE FTYISDIFNV
860 870 880 890 900
QFSMAMCNPD ELMVTYLSRW GLKHWANGVP MYDYPDTETT VAVVNECILL
910 920 930 940 950
LIQLLTEVRS LVMKSSKEGF ERTFKSEIIH ALCFDTCSYA QIVNCIPEHI
960 970 980 990 1000
TKHPSFDIYL EKYANYTSPV SLTDNGIFVL KEKYKDEIDP YYIGLSSSRR
1010 1020 1030 1040 1050
YDVEKNIRLN MANLKKMKYE DTFVPAKKVK DLLKNTLFSG LYSISSVNTF
1060 1070 1080 1090 1100
GLFLKNTLDH IIKYDYDNLL PRVVHLIHLC VVNNLNEFMG ILWHEYAIVD
1110 1120 1130 1140 1150
TEFCHYHSIG SILYYCLLKD NFSESHGKIR EIFRYLMETA PHVNVNSYLR
1160 1170 1180 1190 1200
EQTTSYTPGI LWPTKEDKSH KDKEFERKKH LARLRKKKLM KKLAQQQMKF
1210 1220 1230 1240 1250
MENNSVDTSD ISTPRTTSPS LSPTRINAEN SSNTINSCCD DDCVFCKMPK
1260 1270 1280 1290 1300
DDDVFVYFSY QERNICDHGI DFTNPTDVNR INSLFSGKQT KDSAIQENPQ
1310 1320 1330 1340 1350
DDDGTRLKFT SCEPVLRACG HGSHTKCLSG HMKSIRGIQN QTTKNIPLSY
1360 1370 1380 1390 1400
GSGLIYCPVC NSLSNSFLPK TNDIDKRTSS QFFMCIEKRS EAEENLDPMS
1410 1420 1430 1440 1450
SICIKAAMIL GDLQGKKVTT IEDAYKVVNS VFINTISNTE LRLRSHKKEG
1460 1470 1480 1490 1500
KIVNMERISS QCILTLHLVC ELKSFIYKKF VNSKTFSSEI SRKIWNWNEF
1510 1520 1530 1540 1550
LIKGNNVNLL LYMSQNFDNI DGGKTPQPPN LCIYEMFKRR FHQLLLLLAR
1560 1570 1580 1590 1600
DMMRVNFYKD CRNKIKISSN GSEEPSTSFS YLFNTFKKYV DLFKPDDVRF
1610 1620 1630 1640 1650
DFTSLEKIKD FICSLLLESL SIFCRRTFLL FNIQYDDDGD GDNNNNRSNN
1660 1670 1680 1690 1700
FMDVKQREIE LIFRYFKLPN LTHFLKDFFY NELTQNIERY NDGNDNLRIQ
1710 1720 1730 1740 1750
QVIYDMVQNI NTRAYPSPEH IQLIELPLNL SKFSLDNDEI SNKCDKYEIA
1760 1770 1780 1790 1800
VCLLCGQKCH IQKSIALQGY LQGECTDHMR NGCEITSAYG VFLMTGTNAI
1810 1820 1830 1840 1850
YLSYGKRGTF YAAPYLSKYG ETNEDYKFGT PVYLNRARYA NLANEIVFGN
1860 1870
MIPHIVFRLT DGSADLGGWE TM
Length:1,872
Mass (Da):216,778
Last modified:November 1, 1996 - v1
Checksum:i1F60CC41EB88752F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73196 Genomic DNA. Translation: CAA97547.1.
BK006945 Genomic DNA. Translation: DAA09342.1.
PIRiS64851.
RefSeqiNP_013124.1. NM_001181911.2.

Genome annotation databases

EnsemblFungiiYLR024C; YLR024C; YLR024C.
GeneIDi850711.
KEGGisce:YLR024C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73196 Genomic DNA. Translation: CAA97547.1.
BK006945 Genomic DNA. Translation: DAA09342.1.
PIRiS64851.
RefSeqiNP_013124.1. NM_001181911.2.

3D structure databases

ProteinModelPortaliQ07963.
SMRiQ07963. Positions 93-170.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31298. 161 interactions.
DIPiDIP-6593N.
IntActiQ07963. 4 interactions.
MINTiMINT-694878.

PTM databases

iPTMnetiQ07963.

Proteomic databases

MaxQBiQ07963.
PeptideAtlasiQ07963.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR024C; YLR024C; YLR024C.
GeneIDi850711.
KEGGisce:YLR024C.

Organism-specific databases

EuPathDBiFungiDB:YLR024C.
SGDiS000004014. UBR2.

Phylogenomic databases

GeneTreeiENSGT00530000063055.
HOGENOMiHOG000141750.
InParanoidiQ07963.
OMAiKYSMREF.
OrthoDBiEOG7GBG57.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciYEAST:G3O-32185-MONOMER.

Miscellaneous databases

NextBioi966769.
PROiQ07963.

Family and domain databases

InterProiIPR003126. Znf_UBR.
[Graphical view]
PfamiPF02207. zf-UBR. 1 hit.
[Graphical view]
SMARTiSM00396. ZnF_UBR1. 1 hit.
[Graphical view]
PROSITEiPS51157. ZF_UBR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "The E2-E3 interaction in the N-end rule pathway: the RING-H2 finger of E3 is required for the synthesis of multiubiquitin chain."
    Xie Y., Varshavsky A.
    EMBO J. 18:6832-6844(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH UBC2.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-709.
    Strain: SUB592.
  7. "Rpn4 is a physiological substrate of the Ubr2 ubiquitin ligase."
    Wang L., Mao X., Ju D., Xie Y.
    J. Biol. Chem. 279:55218-55223(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH UBC2 AND RPN4, FUNCTION.
  8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1222, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  9. "Genome-wide analysis identifies MYND-domain protein Mub1 as an essential factor for Rpn4 ubiquitylation."
    Ju D., Wang X., Xu H., Xie Y.
    Mol. Cell. Biol. 28:1404-1412(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MUB1.
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1218 AND SER-1222, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiUBR2_YEAST
AccessioniPrimary (citable) accession number: Q07963
Secondary accession number(s): D6VY26
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 49 molecules/cell in log phase SD medium.1 Publication
Deletion produces no detectable phenotype.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.