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Protein

Rho GTPase-activating protein 1

Gene

ARHGAP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTPase activator for the Rho, Rac and Cdc42 proteins, converting them to the putatively inactive GDP-bound state. Cdc42 seems to be the preferred substrate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei282Involved in G-protein binding to GAPsCurated1

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL
  • GTPase activator activity Source: MGI
  • SH3/SH2 adaptor activity Source: ProtInc

GO - Biological processi

  • regulation of small GTPase mediated signal transduction Source: Reactome
  • Rho protein signal transduction Source: ProtInc
  • small GTPase mediated signal transduction Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Enzyme and pathway databases

BioCyciZFISH:ENSG00000175220-MONOMER.
ReactomeiR-HSA-194840. Rho GTPase cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Rho GTPase-activating protein 1
Alternative name(s):
CDC42 GTPase-activating protein
GTPase-activating protein rhoGAP
Rho-related small GTPase protein activator
Rho-type GTPase-activating protein 1
p50-RhoGAP
Gene namesi
Name:ARHGAP1
Synonyms:CDC42GAP, RHOGAP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:673. ARHGAP1.

Subcellular locationi

GO - Cellular componenti

  • cell-cell adherens junction Source: BHF-UCL
  • cytoplasm Source: BHF-UCL
  • cytosol Source: Reactome
  • extracellular exosome Source: UniProtKB
  • perinuclear region of cytoplasm Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi392.
OpenTargetsiENSG00000175220.
PharmGKBiPA24956.

Polymorphism and mutation databases

DMDMi3024550.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000567001 – 439Rho GTPase-activating protein 1Add BLAST439

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei44PhosphoserineBy similarity1
Modified residuei47PhosphoserineCombined sources1
Modified residuei50PhosphoserineCombined sources1
Modified residuei51PhosphoserineCombined sources1
Modified residuei65PhosphotyrosineBy similarity1
Modified residuei80N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ07960.
MaxQBiQ07960.
PaxDbiQ07960.
PeptideAtlasiQ07960.
PRIDEiQ07960.

2D gel databases

OGPiQ07960.

PTM databases

iPTMnetiQ07960.
PhosphoSitePlusiQ07960.
SwissPalmiQ07960.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiENSG00000175220.
CleanExiHS_ARHGAP1.
ExpressionAtlasiQ07960. baseline and differential.
GenevisibleiQ07960. HS.

Organism-specific databases

HPAiHPA004689.
HPA008285.

Interactioni

Subunit structurei

Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. Interacts with BNIPL.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC42P609532EBI-602762,EBI-81752
CDC42P60953-22EBI-602762,EBI-287394
RHOAP615862EBI-602762,EBI-446668

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL
  • SH3/SH2 adaptor activity Source: ProtInc

Protein-protein interaction databases

BioGridi106885. 36 interactors.
DIPiDIP-6081N.
IntActiQ07960. 15 interactors.
MINTiMINT-5006067.
STRINGi9606.ENSP00000310491.

Structurei

Secondary structure

1439
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi242 – 244Combined sources3
Helixi246 – 252Combined sources7
Helixi261 – 273Combined sources13
Turni278 – 282Combined sources5
Helixi287 – 298Combined sources12
Helixi305 – 307Combined sources3
Helixi312 – 324Combined sources13
Beta strandi325 – 327Combined sources3
Helixi332 – 334Combined sources3
Helixi335 – 339Combined sources5
Helixi341 – 343Combined sources3
Helixi346 – 348Combined sources3
Helixi349 – 357Combined sources9
Helixi362 – 380Combined sources19
Helixi382 – 385Combined sources4
Helixi389 – 400Combined sources12
Helixi406 – 411Combined sources6
Helixi413 – 425Combined sources13
Helixi427 – 430Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AM4X-ray2.70A/B/C233-431[»]
1GRNX-ray2.10B237-439[»]
1OW3X-ray1.80A198-439[»]
1RGPX-ray2.00A198-439[»]
1TX4X-ray1.65A234-431[»]
2NGRX-ray1.90B206-439[»]
DisProtiDP00459.
ProteinModelPortaliQ07960.
SMRiQ07960.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ07960.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini63 – 218CRAL-TRIOPROSITE-ProRule annotationAdd BLAST156
Domaini244 – 431Rho-GAPPROSITE-ProRule annotationAdd BLAST188

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi228 – 238SH3-bindingAdd BLAST11

Sequence similaritiesi

Contains 1 CRAL-TRIO domain.PROSITE-ProRule annotation
Contains 1 Rho-GAP domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3-binding

Phylogenomic databases

eggNOGiKOG4406. Eukaryota.
ENOG410XR4J. LUCA.
GeneTreeiENSGT00420000029688.
HOGENOMiHOG000231442.
HOVERGENiHBG054433.
InParanoidiQ07960.
KOiK18470.
OMAiLIYFHHG.
OrthoDBiEOG091G07HT.
PhylomeDBiQ07960.
TreeFamiTF324164.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
3.40.525.10. 1 hit.
InterProiIPR001251. CRAL-TRIO_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamiPF13716. CRAL_TRIO_2. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTiSM00324. RhoGAP. 1 hit.
SM00516. SEC14. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF52087. SSF52087. 1 hit.
PROSITEiPS50191. CRAL_TRIO. 1 hit.
PS50238. RHOGAP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q07960-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPLSELQDD LTLDDTSEAL NQLKLASIDE KNWPSDEMPD FPKSDDSKSS
60 70 80 90 100
SPELVTHLKW DDPYYDIARH QIVEVAGDDK YGRKIIVFSA CRMPPSHQLD
110 120 130 140 150
HSKLLGYLKH TLDQYVESDY TLLYLHHGLT SDNKPSLSWL RDAYREFDRK
160 170 180 190 200
YKKNIKALYI VHPTMFIKTL LILFKPLISF KFGQKIFYVN YLSELSEHVK
210 220 230 240 250
LEQLGIPRQV LKYDDFLKST QKSPATAPKP MPPRPPLPNQ QFGVSLQHLQ
260 270 280 290 300
EKNPEQEPIP IVLRETVAYL QAHALTTEGI FRRSANTQVV REVQQKYNMG
310 320 330 340 350
LPVDFDQYNE LHLPAVILKT FLRELPEPLL TFDLYPHVVG FLNIDESQRV
360 370 380 390 400
PATLQVLQTL PEENYQVLRF LTAFLVQISA HSDQNKMTNT NLAVVFGPNL
410 420 430
LWAKDAAITL KAINPINTFT KFLLDHQGEL FPSPDPSGL
Length:439
Mass (Da):50,436
Last modified:November 1, 1996 - v1
Checksum:i4DD0CC4419849C35
GO

Sequence cautioni

The sequence AAA16142 differs from that shown. Reason: Erroneous initiation.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_049137369R → C.Corresponds to variant rs11822837dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02570 mRNA. Translation: AAA16142.1. Different initiation.
Z23024 mRNA. Translation: CAA80560.1.
CH471064 Genomic DNA. Translation: EAW67983.1.
CH471064 Genomic DNA. Translation: EAW67984.1.
BC018118 mRNA. Translation: AAH18118.1.
CCDSiCCDS7922.1.
PIRiA49678.
RefSeqiNP_004299.1. NM_004308.3.
XP_011518397.1. XM_011520095.1.
UniGeneiHs.138860.

Genome annotation databases

EnsembliENST00000311956; ENSP00000310491; ENSG00000175220.
GeneIDi392.
KEGGihsa:392.
UCSCiuc001ndd.5. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02570 mRNA. Translation: AAA16142.1. Different initiation.
Z23024 mRNA. Translation: CAA80560.1.
CH471064 Genomic DNA. Translation: EAW67983.1.
CH471064 Genomic DNA. Translation: EAW67984.1.
BC018118 mRNA. Translation: AAH18118.1.
CCDSiCCDS7922.1.
PIRiA49678.
RefSeqiNP_004299.1. NM_004308.3.
XP_011518397.1. XM_011520095.1.
UniGeneiHs.138860.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AM4X-ray2.70A/B/C233-431[»]
1GRNX-ray2.10B237-439[»]
1OW3X-ray1.80A198-439[»]
1RGPX-ray2.00A198-439[»]
1TX4X-ray1.65A234-431[»]
2NGRX-ray1.90B206-439[»]
DisProtiDP00459.
ProteinModelPortaliQ07960.
SMRiQ07960.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106885. 36 interactors.
DIPiDIP-6081N.
IntActiQ07960. 15 interactors.
MINTiMINT-5006067.
STRINGi9606.ENSP00000310491.

PTM databases

iPTMnetiQ07960.
PhosphoSitePlusiQ07960.
SwissPalmiQ07960.

Polymorphism and mutation databases

DMDMi3024550.

2D gel databases

OGPiQ07960.

Proteomic databases

EPDiQ07960.
MaxQBiQ07960.
PaxDbiQ07960.
PeptideAtlasiQ07960.
PRIDEiQ07960.

Protocols and materials databases

DNASUi392.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000311956; ENSP00000310491; ENSG00000175220.
GeneIDi392.
KEGGihsa:392.
UCSCiuc001ndd.5. human.

Organism-specific databases

CTDi392.
DisGeNETi392.
GeneCardsiARHGAP1.
HGNCiHGNC:673. ARHGAP1.
HPAiHPA004689.
HPA008285.
MIMi602732. gene.
neXtProtiNX_Q07960.
OpenTargetsiENSG00000175220.
PharmGKBiPA24956.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4406. Eukaryota.
ENOG410XR4J. LUCA.
GeneTreeiENSGT00420000029688.
HOGENOMiHOG000231442.
HOVERGENiHBG054433.
InParanoidiQ07960.
KOiK18470.
OMAiLIYFHHG.
OrthoDBiEOG091G07HT.
PhylomeDBiQ07960.
TreeFamiTF324164.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000175220-MONOMER.
ReactomeiR-HSA-194840. Rho GTPase cycle.

Miscellaneous databases

ChiTaRSiARHGAP1. human.
EvolutionaryTraceiQ07960.
GeneWikiiARHGAP1.
GenomeRNAii392.
PROiQ07960.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000175220.
CleanExiHS_ARHGAP1.
ExpressionAtlasiQ07960. baseline and differential.
GenevisibleiQ07960. HS.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
3.40.525.10. 1 hit.
InterProiIPR001251. CRAL-TRIO_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamiPF13716. CRAL_TRIO_2. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTiSM00324. RhoGAP. 1 hit.
SM00516. SEC14. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF52087. SSF52087. 1 hit.
PROSITEiPS50191. CRAL_TRIO. 1 hit.
PS50238. RHOGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRHG01_HUMAN
AccessioniPrimary (citable) accession number: Q07960
Secondary accession number(s): D3DQQ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 176 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.