Q07951 (POC3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proteasome chaperone 3

Alternative name(s):
Increased recombination centers protein 25

Gene names

Name:	IRC25
Synonyms:	DMP2, POC3
Ordered Locus Names:	YLR021W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	179 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in 20S proteasome assembly, facilitating the alpha-ring formation. Ref.5 Ref.6
Subunit structure	Component of the 20S proteasome chaperone. Forms a heterodimer with POC4 that binds to proteasome precursors. Interacts with POP2. Ref.5
Miscellaneous	Present with 1820 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the PSMG3 family.

Ontologies

Keywords
Molecular function	Chaperone
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	chaperone-mediated protein complex assembly Inferred from physical interaction PubMed 18278055. Source: SGD nuclear-transcribed mRNA catabolic process, non-stop decay Inferred from mutant phenotype PubMed 17660569. Source: SGD proteasome assembly Inferred from mutant phenotype Ref.5PubMed 18260085PubMed 18278055. Source: SGD
Cellular_component	intracellular Inferred from direct assay Ref.5. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 179

179

Proteasome chaperone 3

PRO_0000247202

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

179

Helix Strand Turn

Details...

Beta strand

3 – 10

8

Beta strand

26 – 33

8

Beta strand

38 – 44

7

Beta strand

50 – 56

7

Helix

84 – 91

8

Beta strand

95 – 102

8

Helix

109 – 122

14

Beta strand

140 – 145

6

Helix

147 – 149

3

Helix

162 – 176

15

Sequences

	Sequence		Length	Mass (Da)
	Q07951 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 6CA25E8ED0491EE2	FASTA	179	20,061
	10 20 30 40 50 60 MISYEFQTHL PKGKDSSLNA SSENKELYVQ ATHFNNTILL QIRLNGEMDS TYEVSSKGLN 70 80 90 100 110 120 PILDINVPLA GNLGNTGGDY DDEEEEFVRD HLSDYQVVTK LGDSADPKVP VVCVQIAELY 130 140 150 160 170 RRVILPEVSG TMAQDNMQFS LLISMSSKIW RATKEQSADD NDFGKLVFVL KCIKDMYAK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"20S proteasome assembly is orchestrated by two distinct pairs of chaperones in yeast and in mammals." Le Tallec B., Barrault M.-B., Courbeyrette R., Guerois R., Marsolier-Kergoat M.-C., Peyroche A. Mol. Cell 27:660-674(2007) [PubMed] [Europe PMC] [Abstract] Cited for: GENE NAME, FUNCTION, INTERACTION WITH POC4, SUBUNIT.
[6]	"Crystal structure of a chaperone complex that contributes to the assembly of yeast 20S proteasomes." Yashiroda H., Mizushima T., Okamoto K., Kameyama T., Hayashi H., Kishimoto T., Niwa S., Kasahara M., Kurimoto E., Sakata E., Takagi K., Suzuki A., Hirano Y., Murata S., Kato K., Yamane T., Tanaka K. Nat. Struct. Mol. Biol. 15:228-236(2008) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.96 ANGSTROMS) IN COMPLEX WITH POC4 AND PUP2, FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z73193 Genomic DNA. Translation: CAA97544.1.
AY558201 Genomic DNA. Translation: AAS56527.1.
BK006945 Genomic DNA. Translation: DAA09339.1.

PIR

S64843.

RefSeq

NP_013121.1. NM_001181908.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2Z5B	X-ray	1.96	B	1-179	[»]
2Z5C	X-ray	2.90	B/E	1-179	[»]

ProteinModelPortal

Q07951.

SMR

Q07951. Positions 1-178.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-4747N.

IntAct

Q07951. 3 interactions.

MINT

MINT-550062.

STRING

4932.YLR021W.

Proteomic databases

PaxDb

Q07951.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YLR021W; YLR021W; YLR021W.

GeneID

850708.

KEGG

sce:YLR021W.

Organism-specific databases

CYGD

YLR021w.

SGD

S000004011. IRC25.

Phylogenomic databases

eggNOG

NOG47807.

HOGENOM

HOG000115599.

KO

K11881.

OMA

EMDSTYE.

OrthoDB

EOG4NKG5C.

Enzyme and pathway databases

BioCyc

YEAST:G3O-32182-MONOMER.

Gene expression databases

Genevestigator

Q07951.

GermOnline

YLR021W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR018854. Psome_chaperone_3/4_fun.
[Graphical view]

Pfam

PF10448. chaperone_DMP. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q07951.

NextBio

966760.

Entry information

Entry name

POC3_YEAST

Accession

Primary (citable) accession number: Q07951
Secondary accession number(s): D6VY23

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	November 1, 1996
Last modified:	May 29, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program