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Protein

Sterol esterase 2

Gene

YEH2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the hydrolysis of steryl esters. Required for mobilization of steryl ester, thereby playing a central role in lipid metabolism.2 Publications

Catalytic activityi

A steryl ester + H2O = a sterol + a fatty acid.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei287 – 2871NucleophileBy similarity
Active sitei480 – 4801Charge relay systemBy similarity
Active sitei511 – 5111Charge relay systemBy similarity

GO - Molecular functioni

  • sterol esterase activity Source: SGD

GO - Biological processi

  • cell wall mannoprotein biosynthetic process Source: SGD
  • lipid catabolic process Source: UniProtKB-KW
  • sterol metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:G3O-32181-MONOMER.
YEAST:G3O-32181-MONOMER.
BRENDAi3.1.1.13. 984.

Protein family/group databases

ESTHERiyeast-YLR020C. Acidic_Lipase.

Names & Taxonomyi

Protein namesi
Recommended name:
Sterol esterase 2 (EC:3.1.1.13)
Alternative name(s):
Steryl ester hydrolase 2
Gene namesi
Name:YEH2
Ordered Locus Names:YLR020C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR020C.
SGDiS000004010. YEH2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1111CytoplasmicSequence analysisAdd
BLAST
Transmembranei12 – 3221Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini33 – 538506LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: SGD
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 538538Sterol esterase 2PRO_0000248405Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei73 – 731PhosphoserineCombined sources
Modified residuei107 – 1071PhosphoserineCombined sources

Post-translational modificationi

Not glycosylated.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ07950.

PTM databases

iPTMnetiQ07950.

Interactioni

Protein-protein interaction databases

BioGridi31294. 14 interactions.
IntActiQ07950. 2 interactions.
MINTiMINT-6673689.

Structurei

3D structure databases

ProteinModelPortaliQ07950.
SMRiQ07950. Positions 157-322.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AB hydrolase superfamily.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00550000076072.
HOGENOMiHOG000141749.
InParanoidiQ07950.
OMAiEYWDWDI.
OrthoDBiEOG789CMC.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR006693. AB_hydrolase_lipase.
[Graphical view]
PfamiPF04083. Abhydro_lipase. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 2 hits.

Sequencei

Sequence statusi: Complete.

Q07950-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVNKVVDEVQ RLVSAIILTS FMTGLFILSL WKNYVTVHFQ HKNDPRDTRS
60 70 80 90 100
SRTKIQPNDK KKKRPARHSR PLSISSTTPL DLQRDQENNI EYDRTVTSKL
110 120 130 140 150
SMTSNASLSE NGDGNANIKM ETNVNQAPYA AENPFQNIAL AEDTKLVPDL
160 170 180 190 200
KYYYKEYGID IEEFEVETDD GFIIDLWHFK SRLNDGVEEV KREPILLLHG
210 220 230 240 250
LLQSCGAFAS SGRKSLAYFL YESGFDVWLG NNRCGLNAKW NMKKLGNDHS
260 270 280 290 300
KKWDWDMHQM VQYDLKALIN YVLDSTGYAK LSLVAHSQGT TQGFMGLVNG
310 320 330 340 350
EKLYASDFKL VDKLENFVAL APAVYPGPLL DEKAFVRLMA KGIDSPWYFG
360 370 380 390 400
RRSFIPLMMT MRKLMVGTKI FSFLSYIMFN YLFDWNDVLW DRVLRDRNFL
410 420 430 440 450
FSPVHISVKL MQWWLSPLPN KLSFKKGAEK IFPDKKTWFP IAKNDDDSGN
460 470 480 490 500
NLDNNKLHLN PKRQNSEEFP HIIMFIPKQD RLVDGERLIN HFINHEANAV
510 520 530
YKIWYIDEYS HLDVLWAHDV IDRIGKPMIE NLRFPNAR
Length:538
Mass (Da):62,447
Last modified:November 1, 1996 - v1
Checksum:i44CFD03B52961CD2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73192 Genomic DNA. Translation: CAA97543.1.
BK006945 Genomic DNA. Translation: DAA09338.1.
PIRiS64842.
RefSeqiNP_013120.1. NM_001181907.1.

Genome annotation databases

EnsemblFungiiYLR020C; YLR020C; YLR020C.
GeneIDi850707.
KEGGisce:YLR020C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73192 Genomic DNA. Translation: CAA97543.1.
BK006945 Genomic DNA. Translation: DAA09338.1.
PIRiS64842.
RefSeqiNP_013120.1. NM_001181907.1.

3D structure databases

ProteinModelPortaliQ07950.
SMRiQ07950. Positions 157-322.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31294. 14 interactions.
IntActiQ07950. 2 interactions.
MINTiMINT-6673689.

Protein family/group databases

ESTHERiyeast-YLR020C. Acidic_Lipase.

PTM databases

iPTMnetiQ07950.

Proteomic databases

MaxQBiQ07950.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR020C; YLR020C; YLR020C.
GeneIDi850707.
KEGGisce:YLR020C.

Organism-specific databases

EuPathDBiFungiDB:YLR020C.
SGDiS000004010. YEH2.

Phylogenomic databases

GeneTreeiENSGT00550000076072.
HOGENOMiHOG000141749.
InParanoidiQ07950.
OMAiEYWDWDI.
OrthoDBiEOG789CMC.

Enzyme and pathway databases

BioCyciMetaCyc:G3O-32181-MONOMER.
YEAST:G3O-32181-MONOMER.
BRENDAi3.1.1.13. 984.

Miscellaneous databases

NextBioi966757.
PROiQ07950.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR006693. AB_hydrolase_lipase.
[Graphical view]
PfamiPF04083. Abhydro_lipase. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  4. "YEH2/YLR020c encodes a novel steryl ester hydrolase of the yeast Saccharomyces cerevisiae."
    Muellner H., Deutsch G., Leitner E., Ingolic E., Daum G.
    J. Biol. Chem. 280:13321-13328(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  5. "The Saccharomyces cerevisiae YLL012/YEH1, YLR020/YEH2, and TGL1 genes encode a novel family of membrane-anchored lipases that are required for steryl ester hydrolysis."
    Koeffel R., Tiwari R., Falquet L., Schneiter R.
    Mol. Cell. Biol. 25:1655-1668(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, LACK OF GLYCOSYLATION, MEMBRANE TOPOLOGY.
  6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-73, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-73, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-73 AND SER-107, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiYEH2_YEAST
AccessioniPrimary (citable) accession number: Q07950
Secondary accession number(s): D6VY22
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1630 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.