ID   BEDC2_PSEPU             Reviewed;         187 AA.
AC   Q07945;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-MAR-2009, entry version 47.
DE   RecName: Full=Benzene 1,2-dioxygenase subunit beta;
DE            EC=1.14.12.3;
GN   Name=bedC2;
OS   Pseudomonas putida.
OG   Plasmid pHMT112.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-15.
RC   STRAIN=ML2;
RX   MEDLINE=93345820; PubMed=8344526; DOI=10.1016/0378-1119(93)90343-2;
RA   Tan H.-M., Tang H.-Y., Joannou C., Abdel-Wahab N.H., Mason J.R.;
RT   "The Pseudomonas putida ML2 plasmid-encoded genes for benzene
RT   dioxygenase are unusual in codon usage and low in G+C content.";
RL   Gene 130:33-39(1993).
CC   -!- FUNCTION: The beta subunit may be responsible for the substrate
CC       specificity of the enzyme.
CC   -!- CATALYTIC ACTIVITY: Benzene + NADH + O(2) = cis-cyclohexa-3,5-
CC       diene-1,2-diol + NAD(+).
CC   -!- PATHWAY: Aromatic compound metabolism; benzene degradation;
CC       catechol from benzene: step 1/2.
CC   -!- SUBUNIT: This dioxygenase system consists of four proteins: the
CC       two subunits of the hydroxylase component (bedC1 and bedC2), a
CC       ferredoxin (bedB) and a ferredoxin reductase (bedA).
CC   -!- SIMILARITY: Belongs to the bacterial ring-hydroxylating
CC       dioxygenase beta subunit family.
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DR   EMBL; AF148496; AAA17759.1; -; Genomic_DNA.
DR   PIR; JN0813; JN0813.
DR   SMR; Q07945; 11-187.
DR   BioCyc; MetaCyc:MON-12881; -.
DR   BRENDA; 1.14.12.3; 403.
DR   GO; GO:0018619; F:benzene 1,2-dioxygenase activity; IEA:EC.
DR   GO; GO:0016702; F:oxidoreductase activity, acting on single d...; IEA:UniProtKB-KW.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR000391; Rng_hydr_dOase-bsu.
DR   Pfam; PF00866; Ring_hydroxyl_B; 1.
PE   1: Evidence at protein level;
KW   Aromatic hydrocarbons catabolism; Dioxygenase;
KW   Direct protein sequencing; NAD; Oxidoreductase; Plasmid.
FT   CHAIN         1    187       Benzene 1,2-dioxygenase subunit beta.
FT                                /FTId=PRO_0000085066.
SQ   SEQUENCE   187 AA;  22253 MW;  ECF709BE6DF2B2BD CRC64;
     MIDSVNRADL FLRKPAPVAL ELQNEIEQFY YWEAKLLNDR RFDEWFALLA KDIHYFMPIR
     TTRIMRDSRL EYSGLRDYAH FDDDATMMKG RLRKITSDVS WSENPASRTR HIVSNVMIIP
     TEVEGEYEIS STFIVYRNRL ERQLDIFAGE RRDRLRRNKG EAGFEIVNRT ILIDQSTILA
     NNLSFFF
//