Reviewed, UniProtKB/Swiss-Prot Q07945 (BEDC2_PSEPU)

Names and origin

Protein names

Recommended name:
    Benzene 1,2-dioxygenase subunit beta
    EC=1.14.12.3

Gene names

Name:

bedC2

Encoded on

Plasmid pHMT112

Organism

Pseudomonas putida

Taxonomic identifier

303 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	187 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The beta subunit may be responsible for the substrate specificity of the enzyme.
Catalytic activity	Benzene + NADH + O2 = cis-cyclohexa-3,5-diene-1,2-diol + NAD+.
Pathway	Aromatic compound metabolism; benzene degradation; catechol from benzene: step 1/2.
Subunit structure	This dioxygenase system consists of four proteins: the two subunits of the hydroxylase component (bedC1 and bedC2), a ferredoxin (bedB) and a ferredoxin reductase (bedA).
Sequence similarities	Belongs to the bacterial ring-hydroxylating dioxygenase beta subunit family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	NAD
Molecular function	Dioxygenase Oxidoreductase
Technical term	Direct protein sequencing Plasmid
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	benzene 1,2-dioxygenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 187	187	Benzene 1,2-dioxygenase subunit beta		PRO_0000085066

Sequences

	Sequence		Length	Mass (Da)
	Q07945-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: ECF709BE6DF2B2BD	FASTA	187	22,253
	10 20 30 40 50 60 MIDSVNRADL FLRKPAPVAL ELQNEIEQFY YWEAKLLNDR RFDEWFALLA KDIHYFMPIR 70 80 90 100 110 120 TTRIMRDSRL EYSGLRDYAH FDDDATMMKG RLRKITSDVS WSENPASRTR HIVSNVMIIP 130 140 150 160 170 180 TEVEGEYEIS STFIVYRNRL ERQLDIFAGE RRDRLRRNKG EAGFEIVNRT ILIDQSTILA NNLSFFF

References

[1]

"The Pseudomonas putida ML2 plasmid-encoded genes for benzene dioxygenase are unusual in codon usage and low in G+C content." Tan H.-M., Tang H.-Y., Joannou C., Abdel-Wahab N.H., Mason J.R. Gene 130:33-39(1993) [PubMed: 8344526] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-15. Strain: ML2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF148496 Genomic DNA. Translation: AAA17759.1.
PIR	JN0813.
3D structure databases
SMR	Q07945. Positions 11-187.
ModBase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MON-12881.
BRENDA	1.14.12.3. 403.
Family and domain databases
InterPro	IPR000391. Rng_hydr_dOase-bsu. [Graphical view]
Pfam	PF00866. Ring_hydroxyl_B. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BEDC2_PSEPU

Accession

Primary (citable) accession number: Q07945

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	March 3, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)