ID   BEDC1_PSEPU             Reviewed;         450 AA.
AC   Q07944;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   16-JUN-2009, entry version 56.
DE   RecName: Full=Benzene 1,2-dioxygenase subunit alpha;
DE            EC=1.14.12.3;
GN   Name=bedC1;
OS   Pseudomonas putida.
OG   Plasmid pHMT112.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-15.
RC   STRAIN=ML2;
RX   MEDLINE=93345820; PubMed=8344526; DOI=10.1016/0378-1119(93)90343-2;
RA   Tan H.-M., Tang H.-Y., Joannou C., Abdel-Wahab N.H., Mason J.R.;
RT   "The Pseudomonas putida ML2 plasmid-encoded genes for benzene
RT   dioxygenase are unusual in codon usage and low in G+C content.";
RL   Gene 130:33-39(1993).
CC   -!- CATALYTIC ACTIVITY: Benzene + NADH + O(2) = cis-cyclohexa-3,5-
CC       diene-1,2-diol + NAD(+).
CC   -!- COFACTOR: Binds 1 2Fe-2S cluster (Probable).
CC   -!- COFACTOR: Binds 1 iron ion (Probable).
CC   -!- PATHWAY: Aromatic compound metabolism; benzene degradation;
CC       catechol from benzene: step 1/2.
CC   -!- SUBUNIT: This dioxygenase system consists of four proteins: the
CC       two subunits of the hydroxylase component (bedC1 and bedC2), a
CC       ferredoxin (bedB) and a ferredoxin reductase (bedA).
CC   -!- SIMILARITY: Belongs to the bacterial ring-hydroxylating
CC       dioxygenase alpha subunit family.
CC   -!- SIMILARITY: Contains 1 Rieske domain.
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DR   EMBL; AF148496; AAA17758.1; -; Genomic_DNA.
DR   PIR; JN0812; JN0812.
DR   HSSP; P23094; 1O7N.
DR   SMR; Q07944; 15-450.
DR   BioCyc; MetaCyc:MON-12880; -.
DR   BRENDA; 1.14.12.3; 403.
DR   GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0018619; F:benzene 1,2-dioxygenase activity; IEA:EC.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016702; F:oxidoreductase activity, acting on single d...; IEA:UniProtKB-KW.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR017941; Rieske_2Fe-2S.
DR   InterPro; IPR015881; Ring-hydroxy_dOase_2Fe2S_BS.
DR   InterPro; IPR015879; Ring_hydroxy_dOase_asu_C.
DR   InterPro; IPR001663; Rng_hydr_dOase-A.
DR   PANTHER; PTHR21266:SF2; Rng_hydr_dOase-A; 1.
DR   Pfam; PF00355; Rieske; 1.
DR   Pfam; PF00848; Ring_hydroxyl_A; 1.
DR   PRINTS; PR00090; RNGDIOXGNASE.
DR   PROSITE; PS51296; RIESKE; 1.
DR   PROSITE; PS00570; RING_HYDROXYL_ALPHA; 1.
PE   1: Evidence at protein level;
KW   2Fe-2S; Aromatic hydrocarbons catabolism; Dioxygenase;
KW   Direct protein sequencing; Iron; Iron-sulfur; Metal-binding; NAD;
KW   Oxidoreductase; Plasmid.
FT   CHAIN         1    450       Benzene 1,2-dioxygenase subunit alpha.
FT                                /FTId=PRO_0000085044.
FT   DOMAIN       56    163       Rieske.
FT   METAL        96     96       Iron-sulfur (2Fe-2S) (By similarity).
FT   METAL        98     98       Iron-sulfur (2Fe-2S); via pros nitrogen
FT                                (By similarity).
FT   METAL       116    116       Iron-sulfur (2Fe-2S) (By similarity).
FT   METAL       119    119       Iron-sulfur (2Fe-2S); via pros nitrogen
FT                                (By similarity).
FT   METAL       222    222       Iron (By similarity).
FT   METAL       228    228       Iron (By similarity).
SQ   SEQUENCE   450 AA;  51109 MW;  1ECD5EA6C4CF72C8 CRC64;
     MNQTETTPIR VRKNWKTSEI ETLFDEQAGR IDPRIYTDED LYQLELERVF ARSWLLLGHE
     THIRKPGDYF TTYMGEDPVV VVRQKDASIA VFLNQCRHRG MRICRSDAGN AKAFTCSYHG
     WAYDTAGNLI NVPYEAESFA CLDKKEWSPL KARVETYKGL IFANWDENAI DLDTYLGEAK
     FYMDHMLDRT EAGTEVIPGI QKWVIPCNWK FAAEQFCSDM YHAGTTAHLS GIIAGLPEDL
     ELADLAPPKF GKQYRASWGG HGSGFYIGDP NMMLAMMGPK VTSYLTEGPA AEKAAERLGS
     IERGTKIMLE HMTVFPTCSF LPGVNTIRTW HPRGPNEVEV WAFTVVDADA PDDIKEEFRR
     QTLRTFSAGG VFEQDDGENW VEIQHILRGH KARSRPFNAE MSMGQTVDND PIYPGRISNN
     VYSEEAARGL YAHWLKMMTS PDWEALKATR
//