Benzene 1,2-dioxygenase subunit alpha - Pseudomonas putida

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Reviewed, UniProtKB/Swiss-Prot Q07944 (BEDC1_PSEPU)

Last modified November 24, 2009. Version 57. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names

Recommended name:
Benzene 1,2-dioxygenase subunit alpha
EC=1.14.12.3

Gene names

Name:

bedC1

Encoded on

Plasmid pHMT112

Organism

Pseudomonas putida

Taxonomic identifier

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Benzene + NADH + O₂ = cis-cyclohexa-3,5-diene-1,2-diol + NAD⁺.
Cofactor	Binds 1 2Fe-2S cluster Probable. Binds 1 iron ion Probable.
Pathway	Aromatic compound metabolism; benzene degradation; catechol from benzene: step 1/2.
Subunit structure	This dioxygenase system consists of four proteins: the two subunits of the hydroxylase component (bedC1 and bedC2), a ferredoxin (bedB) and a ferredoxin reductase (bedA).
Sequence similarities	Belongs to the bacterial ring-hydroxylating dioxygenase alpha subunit family. Contains 1 Rieske domain.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding NAD
Molecular function	Dioxygenase Oxidoreductase
Technical term	Direct protein sequencing Plasmid
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW benzene 1,2-dioxygenase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

450

Benzene 1,2-dioxygenase subunit alpha

PRO_0000085044

Regions

Domain

108

Rieske

Sites

Metal binding

1

Iron-sulfur (2Fe-2S) By similarity

Metal binding

1

Iron-sulfur (2Fe-2S); via pros nitrogen By similarity

Metal binding

1

Iron-sulfur (2Fe-2S) By similarity

Metal binding

1

Iron-sulfur (2Fe-2S); via pros nitrogen By similarity

Metal binding

1

Iron By similarity

Metal binding

1

Iron By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q07944-1 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 1ECD5EA6C4CF72C8
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450

51,109

        10         20         30         40         50         60 
MNQTETTPIR VRKNWKTSEI ETLFDEQAGR IDPRIYTDED LYQLELERVF ARSWLLLGHE 

        70         80         90        100        110        120 
THIRKPGDYF TTYMGEDPVV VVRQKDASIA VFLNQCRHRG MRICRSDAGN AKAFTCSYHG 

       130        140        150        160        170        180 
WAYDTAGNLI NVPYEAESFA CLDKKEWSPL KARVETYKGL IFANWDENAI DLDTYLGEAK 

       190        200        210        220        230        240 
FYMDHMLDRT EAGTEVIPGI QKWVIPCNWK FAAEQFCSDM YHAGTTAHLS GIIAGLPEDL 

       250        260        270        280        290        300 
ELADLAPPKF GKQYRASWGG HGSGFYIGDP NMMLAMMGPK VTSYLTEGPA AEKAAERLGS 

       310        320        330        340        350        360 
IERGTKIMLE HMTVFPTCSF LPGVNTIRTW HPRGPNEVEV WAFTVVDADA PDDIKEEFRR 

       370        380        390        400        410        420 
QTLRTFSAGG VFEQDDGENW VEIQHILRGH KARSRPFNAE MSMGQTVDND PIYPGRISNN 

       430        440        450 
VYSEEAARGL YAHWLKMMTS PDWEALKATR

References

[1]

"The Pseudomonas putida ML2 plasmid-encoded genes for benzene dioxygenase are unusual in codon usage and low in G+C content."
Tan H.-M., Tang H.-Y., Joannou C., Abdel-Wahab N.H., Mason J.R.
Gene 130:33-39(1993) [PubMed: 8344526] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-15.
Strain: ML2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF148496 Genomic DNA. Translation: AAA17758.1.
PIR	JN0812.
3D structure databases
SMR	Q07944. Positions 15-450.
ModBase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-12880.
BRENDA	1.14.12.3. 403.
Family and domain databases
InterPro	IPR017941. Rieske_2Fe-2S. IPR015881. Ring-hydroxy_dOase_2Fe2S_BS. IPR015879. Ring_hydroxy_dOase_asu_C. IPR001663. Rng_hydr_dOase-A. [Graphical view]
PANTHER	PTHR21266:SF2. Rng_hydr_dOase-A. 1 hit.
Pfam	PF00355. Rieske. 1 hit. PF00848. Ring_hydroxyl_A. 1 hit. [Graphical view]
PRINTS	PR00090. RNGDIOXGNASE.
PROSITE	PS51296. RIESKE. 1 hit. PS00570. RING_HYDROXYL_ALPHA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BEDC1_PSEPU

Accession

Primary (citable) accession number: Q07944

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 24, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents