ID   GUN4_RUMAL              Reviewed;         312 AA.
AC   Q07940;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   16-JUN-2009, entry version 54.
DE   RecName: Full=Endoglucanase 4;
DE            EC=3.2.1.4;
DE   AltName: Full=Endoglucanase IV;
DE            Short=EG-IV;
DE   AltName: Full=Endo-1,4-beta-glucanase 4;
DE   AltName: Full=Cellulase 4;
GN   Name=Eg IV;
OS   Ruminococcus albus.
OC   Bacteria; Firmicutes; Clostridia; Clostridiales; Ruminococcaceae;
OC   Ruminococcus.
OX   NCBI_TaxID=1264;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-19.
RC   STRAIN=F-40;
RA   Karita S., Morioka K., Kajino T., Sakka K., Shimada K., Ohmiya K.;
RT   "Cloning and sequencing of a novel endo-1,4-beta-glucanase gene from
RT   Ruminococcus albus.";
RL   J. Ferment. Bioeng. 76:439-444(1993).
CC   -!- CATALYTIC ACTIVITY: Endohydrolysis of (1->4)-beta-D-glucosidic
CC       linkages in cellulose, lichenin and cereal beta-D-glucans.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Optimum pH is 7;
CC       Temperature dependence:
CC         Optimum temperature is 40 degrees Celsius;
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 5 (cellulase A)
CC       family.
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DR   EMBL; AB016777; BAA32286.1; -; Genomic_DNA.
DR   HSSP; Q59232; 1LF1.
DR   CAZy; GH5; Glycoside Hydrolase Family 5.
DR   BRENDA; 3.2.1.4; 1267.
DR   GO; GO:0043169; F:cation binding; IEA:InterPro.
DR   GO; GO:0008810; F:cellulase activity; IEA:EC.
DR   GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR001547; Glyco_hydro_5.
DR   InterPro; IPR018087; Glyco_hydro_5_CS.
DR   InterPro; IPR013781; Glyco_hydro_sg_catalytic.
DR   Gene3D; G3DSA:3.20.20.80; Glyco_hydro_cat; 1.
DR   Pfam; PF00150; Cellulase; 1.
DR   PROSITE; PS00659; GLYCOSYL_HYDROL_F5; 1.
PE   1: Evidence at protein level;
KW   Carbohydrate metabolism; Cellulose degradation;
KW   Direct protein sequencing; Glycosidase; Hydrolase;
KW   Polysaccharide degradation.
FT   CHAIN         1    312       Endoglucanase 4.
FT                                /FTId=PRO_0000184050.
FT   ACT_SITE    135    135       Proton donor (By similarity).
FT   ACT_SITE    222    222       Nucleophile (By similarity).
SQ   SEQUENCE   312 AA;  35767 MW;  A8BAADD9F93A8CBC CRC64;
     MLDKLKVING KLTAGEKPVR LFGLSTHGIA WYPEYICEES FNALKKDWRT NCIRIAMYTD
     EFRGYCKDGN KQHLKELIEK GVVIAEKLDM YVIVDWHVLC DQDPMKYIDE AEEFFSDMSK
     RFANKTNVIY EICNEPNCSG TWDKITEYAD RIIPIIRSNS PDALIVTGTS TWSQDIHCAL
     EKPLKWDNVM YSLHFYAATH KGTLRSRLER CIEAGLPVFI NEFNLCEASG KGDIDIDEAN
     AWYEVIDRLG LSCISWCLSN SGDTCGVFTQ NCTKLSGWTD EDIKTSGKII KGWFEAFADE
     ENTNEQCFRI DK
//