Reviewed, UniProtKB/Swiss-Prot Q07940 (GUN4_RUMAL)

Names and origin

Protein names

Recommended name:
    Endoglucanase 4
    EC=3.2.1.4
Alternative name(s):
    Endoglucanase IV
      Short name=EG-IV
    Endo-1,4-beta-glucanase 4
    Cellulase 4

Gene names

Name:

Eg IV

Organism

Ruminococcus albus

Taxonomic identifier

1264 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Ruminococcaceae › Ruminococcus

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Sequence similarities	Belongs to the glycosyl hydrolase 5 (cellulase A) family.
Biophysicochemical properties	pH dependence: Optimum pH is 7. Temperature dependence: Optimum temperature is 40 degrees Celsius.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Molecular function	Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	Endoglucanase 4		PRO_0000184050
Sites
	Active site	135	1	Proton donor By similarity
	Active site	222	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q07940-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: A8BAADD9F93A8CBC	FASTA	312	35,767
	10 20 30 40 50 60 MLDKLKVING KLTAGEKPVR LFGLSTHGIA WYPEYICEES FNALKKDWRT NCIRIAMYTD 70 80 90 100 110 120 EFRGYCKDGN KQHLKELIEK GVVIAEKLDM YVIVDWHVLC DQDPMKYIDE AEEFFSDMSK 130 140 150 160 170 180 RFANKTNVIY EICNEPNCSG TWDKITEYAD RIIPIIRSNS PDALIVTGTS TWSQDIHCAL 190 200 210 220 230 240 EKPLKWDNVM YSLHFYAATH KGTLRSRLER CIEAGLPVFI NEFNLCEASG KGDIDIDEAN 250 260 270 280 290 300 AWYEVIDRLG LSCISWCLSN SGDTCGVFTQ NCTKLSGWTD EDIKTSGKII KGWFEAFADE 310 ENTNEQCFRI DK

References

[1]	"Cloning and sequencing of a novel endo-1,4-beta-glucanase gene from Ruminococcus albus." Karita S., Morioka K., Kajino T., Sakka K., Shimada K., Ohmiya K. J. Ferment. Bioeng. 76:439-444(1993) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-19. Strain: F-40.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB016777 Genomic DNA. Translation: BAA32286.1.
3D structure databases
SMR	Q07940. Positions 2-294.
ModBase	Search...
Protein family/group databases
CAZy	GH5. Glycoside Hydrolase Family 5.
Enzyme and pathway databases
BRENDA	3.2.1.4. 1267.
Family and domain databases
InterPro	IPR001547. Glyco_hydro_5. IPR018087. Glyco_hydro_5_CS. IPR017853. Glyco_hydro_catalytic_core. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00150. Cellulase. 1 hit. [Graphical view]
PROSITE	PS00659. GLYCOSYL_HYDROL_F5. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN4_RUMAL

Accession

Primary (citable) accession number: Q07940

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	January 19, 2010
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)