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Q07940 (GUN4_RUMAL) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endoglucanase 4

EC=3.2.1.4
Alternative name(s):
Cellulase 4
Endo-1,4-beta-glucanase 4
Endoglucanase IV
Short name=EG-IV
Gene names
Name:Eg IV
OrganismRuminococcus albus
Taxonomic identifier1264 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminococcus

Protein attributes

Sequence length312 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sequence similarities

Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Biophysicochemical properties

pH dependence:

Optimum pH is 7.

Temperature dependence:

Optimum temperature is 40 degrees Celsius.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Cellulose degradation
Polysaccharide degradation
   Molecular functionGlycosidase
Hydrolase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processcellulose catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functioncellulase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 312312Endoglucanase 4
PRO_0000184050

Sites

Active site1351Proton donor By similarity
Active site2221Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
Q07940 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: A8BAADD9F93A8CBC

FASTA31235,767
        10         20         30         40         50         60 
MLDKLKVING KLTAGEKPVR LFGLSTHGIA WYPEYICEES FNALKKDWRT NCIRIAMYTD 

        70         80         90        100        110        120 
EFRGYCKDGN KQHLKELIEK GVVIAEKLDM YVIVDWHVLC DQDPMKYIDE AEEFFSDMSK 

       130        140        150        160        170        180 
RFANKTNVIY EICNEPNCSG TWDKITEYAD RIIPIIRSNS PDALIVTGTS TWSQDIHCAL 

       190        200        210        220        230        240 
EKPLKWDNVM YSLHFYAATH KGTLRSRLER CIEAGLPVFI NEFNLCEASG KGDIDIDEAN 

       250        260        270        280        290        300 
AWYEVIDRLG LSCISWCLSN SGDTCGVFTQ NCTKLSGWTD EDIKTSGKII KGWFEAFADE 

       310 
ENTNEQCFRI DK 

« Hide

References

[1]"Cloning and sequencing of a novel endo-1,4-beta-glucanase gene from Ruminococcus albus."
Karita S., Morioka K., Kajino T., Sakka K., Shimada K., Ohmiya K.
J. Ferment. Bioeng. 76:439-444(1993)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-19.
Strain: F-40.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB016777 Genomic DNA. Translation: BAA32286.1.

3D structure databases

ProteinModelPortalQ07940.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGUN4_RUMAL
AccessionPrimary (citable) accession number: Q07940
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: October 16, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries