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Q07938

- MTAP_YEAST

UniProt

Q07938 - MTAP_YEAST

Protein

S-methyl-5'-thioadenosine phosphorylase

Gene

MEU1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates. Seems to be implicated in the regulation of the expression of the ADH2 gene.4 PublicationsUniRule annotation

    Catalytic activityi

    S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei41 – 411PhosphateUniRule annotation
    Sitei212 – 2121Important for substrate specificityUniRule annotation
    Binding sitei230 – 2301Substrate; via amide nitrogenUniRule annotation
    Binding sitei231 – 2311PhosphateUniRule annotation
    Sitei267 – 2671Important for substrate specificityUniRule annotation

    GO - Molecular functioni

    1. mRNA binding Source: SGD
    2. phosphorylase activity Source: InterPro
    3. S-methyl-5-thioadenosine phosphorylase activity Source: SGD

    GO - Biological processi

    1. glutamate biosynthetic process Source: SGD
    2. L-methionine biosynthetic process from methylthioadenosine Source: SGD
    3. purine ribonucleoside salvage Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine salvage

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32178-MONOMER.
    YEAST:MONOMER3O-157.
    ReactomeiREACT_189018. Methionine salvage pathway.
    UniPathwayiUPA00904; UER00873.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    S-methyl-5'-thioadenosine phosphorylaseUniRule annotation (EC:2.4.2.28UniRule annotation)
    Alternative name(s):
    5'-methylthioadenosine phosphorylaseUniRule annotation
    Short name:
    MTA phosphorylaseUniRule annotation
    Short name:
    MTAPUniRule annotation
    Short name:
    MTAPaseUniRule annotation
    Multicopy enhancer of UAS2
    Gene namesi
    Name:MEU1UniRule annotation
    Ordered Locus Names:YLR017W
    ORF Names:L1595
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    CYGDiYLR017w.
    SGDiS000004007. MEU1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: SGD
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 337337S-methyl-5'-thioadenosine phosphorylasePRO_0000184556Add
    BLAST

    Proteomic databases

    MaxQBiQ07938.
    PaxDbiQ07938.
    PeptideAtlasiQ07938.

    Expressioni

    Gene expression databases

    GenevestigatoriQ07938.

    Interactioni

    Subunit structurei

    Homotrimer.UniRule annotation

    Protein-protein interaction databases

    BioGridi31291. 26 interactions.
    DIPiDIP-989N.
    IntActiQ07938. 2 interactions.
    MINTiMINT-4494408.

    Structurei

    3D structure databases

    ProteinModelPortaliQ07938.
    SMRiQ07938. Positions 30-334.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni88 – 892Phosphate bindingUniRule annotation
    Regioni121 – 1222Phosphate bindingUniRule annotation
    Regioni254 – 2563Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0005.
    GeneTreeiENSGT00550000074874.
    HOGENOMiHOG000228986.
    KOiK00772.
    OMAiHVAFIAR.
    OrthoDBiEOG77DJGM.

    Family and domain databases

    Gene3Di3.40.50.1580. 1 hit.
    HAMAPiMF_01963. MTAP.
    InterProiIPR010044. MTAP.
    IPR000845. Nucleoside_phosphorylase_d.
    IPR001369. PNP/MTAP.
    IPR018099. Purine_phosphorylase-2_CS.
    [Graphical view]
    PANTHERiPTHR11904. PTHR11904. 1 hit.
    PfamiPF01048. PNP_UDP_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF53167. SSF53167. 1 hit.
    PROSITEiPS01240. PNP_MTAP_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q07938-1 [UniParc]FASTAAdd to Basket

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    MNRIKNTFSV AKRLKLSKVM TNSELPSIFE GTVDLGIIGG TGLYNLDCLE    50
    PIALLPPMVT PWGTTSSPVT ISQFVGTNSH FHVAFIARHG INHEYPPTKV 100
    PFRANMAALK NLNCKAVLSF SAVGSLQPHI KPRDFVLPQQ IIDRTKGIRH 150
    SSYFNDEGLV GHVGFGQPFS QKFAEYIYQF KNEITNPESE EPCHLHYDKD 200
    MTVVCMEGPQ FSTRAESKMY RMFGGHVINM SVIPEAKLAR ECELPYQMIC 250
    MSTDYDAWRD EAEPVTVETV IGNLTNNGRN ANILASKIIV SMAKEIPEFM 300
    HTGDGLRGSI KKSISTKPEA MSKETLERLR YLFPNYW 337
    Length:337
    Mass (Da):37,857
    Last modified:November 1, 1997 - v1
    Checksum:i99B54F62ED8A9389
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti55 – 551L → V in CAA62156. 1 PublicationCurated
    Sequence conflicti157 – 1571E → G in AAT93089. (PubMed:17322287)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X90564 Genomic DNA. Translation: CAA62156.1.
    Z73189 Genomic DNA. Translation: CAA97539.1.
    AY693070 Genomic DNA. Translation: AAT93089.1.
    BK006945 Genomic DNA. Translation: DAA09335.1.
    PIRiS64839.
    RefSeqiNP_013117.1. NM_001181904.1.

    Genome annotation databases

    EnsemblFungiiYLR017W; YLR017W; YLR017W.
    GeneIDi850704.
    KEGGisce:YLR017W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X90564 Genomic DNA. Translation: CAA62156.1 .
    Z73189 Genomic DNA. Translation: CAA97539.1 .
    AY693070 Genomic DNA. Translation: AAT93089.1 .
    BK006945 Genomic DNA. Translation: DAA09335.1 .
    PIRi S64839.
    RefSeqi NP_013117.1. NM_001181904.1.

    3D structure databases

    ProteinModelPortali Q07938.
    SMRi Q07938. Positions 30-334.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31291. 26 interactions.
    DIPi DIP-989N.
    IntActi Q07938. 2 interactions.
    MINTi MINT-4494408.

    Proteomic databases

    MaxQBi Q07938.
    PaxDbi Q07938.
    PeptideAtlasi Q07938.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLR017W ; YLR017W ; YLR017W .
    GeneIDi 850704.
    KEGGi sce:YLR017W.

    Organism-specific databases

    CYGDi YLR017w.
    SGDi S000004007. MEU1.

    Phylogenomic databases

    eggNOGi COG0005.
    GeneTreei ENSGT00550000074874.
    HOGENOMi HOG000228986.
    KOi K00772.
    OMAi HVAFIAR.
    OrthoDBi EOG77DJGM.

    Enzyme and pathway databases

    UniPathwayi UPA00904 ; UER00873 .
    BioCyci YEAST:G3O-32178-MONOMER.
    YEAST:MONOMER3O-157.
    Reactomei REACT_189018. Methionine salvage pathway.

    Miscellaneous databases

    NextBioi 966748.
    PROi Q07938.

    Gene expression databases

    Genevestigatori Q07938.

    Family and domain databases

    Gene3Di 3.40.50.1580. 1 hit.
    HAMAPi MF_01963. MTAP.
    InterProi IPR010044. MTAP.
    IPR000845. Nucleoside_phosphorylase_d.
    IPR001369. PNP/MTAP.
    IPR018099. Purine_phosphorylase-2_CS.
    [Graphical view ]
    PANTHERi PTHR11904. PTHR11904. 1 hit.
    Pfami PF01048. PNP_UDP_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53167. SSF53167. 1 hit.
    PROSITEi PS01240. PNP_MTAP_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A 7.8kb fragment from chromosome XII of Saccharomyces cerevisiae does not harbour PKC2."
      Saville S.P., Atkinson S., Jamieson L., Pocklington M.J., Orr E.
      Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Isolation and identification of genes activating UAS2-dependent ADH2 expression in Saccharomyces cerevisiae."
      Donoviel M.S., Young E.T.
      Genetics 143:1137-1148(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    6. "Methylthioadenosine phosphorylase regulates ornithine decarboxylase by production of downstream metabolites."
      Subhi A.L., Diegelman P., Porter C.W., Tang B., Lu Z.J., Markham G.D., Kruger W.D.
      J. Biol. Chem. 278:49868-49873(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Methylthioadenosine and polyamine biosynthesis in a Saccharomyces cerevisiae meu1delta mutant."
      Chattopadhyay M.K., Tabor C.W., Tabor H.
      Biochem. Biophys. Res. Commun. 343:203-207(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    10. "A complete inventory of all enzymes in the eukaryotic methionine salvage pathway."
      Pirkov I., Norbeck J., Gustafsson L., Albers E.
      FEBS J. 275:4111-4120(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiMTAP_YEAST
    AccessioniPrimary (citable) accession number: Q07938
    Secondary accession number(s): D6VY19, E9P915, Q06899
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 4820 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3