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Protein

S-methyl-5'-thioadenosine phosphorylase

Gene

MEU1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates. Seems to be implicated in the regulation of the expression of the ADH2 gene.UniRule annotation4 Publications

Miscellaneous

Present with 4820 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation

Pathwayi: L-methionine biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. S-methyl-5'-thioadenosine phosphorylase (MEU1)
This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route), the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei41PhosphateUniRule annotation1
Sitei212Important for substrate specificityUniRule annotation1
Binding sitei230Substrate; via amide nitrogenUniRule annotation1
Binding sitei231PhosphateUniRule annotation1
Sitei267Important for substrate specificityUniRule annotation1

GO - Molecular functioni

  • mRNA binding Source: SGD
  • S-methyl-5-thioadenosine phosphorylase activity Source: SGD

GO - Biological processi

  • glutamate biosynthetic process Source: SGD
  • L-methionine salvage from methylthioadenosine Source: SGD
  • purine ribonucleoside salvage Source: UniProtKB-KW

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processPurine salvage

Enzyme and pathway databases

BioCyciYEAST:MONOMER3O-157
ReactomeiR-SCE-1237112 Methionine salvage pathway
UniPathwayiUPA00904; UER00873

Names & Taxonomyi

Protein namesi
Recommended name:
S-methyl-5'-thioadenosine phosphorylaseUniRule annotation (EC:2.4.2.28UniRule annotation)
Alternative name(s):
5'-methylthioadenosine phosphorylaseUniRule annotation
Short name:
MTA phosphorylaseUniRule annotation
Short name:
MTAPUniRule annotation
Short name:
MTAPaseUniRule annotation
Multicopy enhancer of UAS2
Gene namesi
Name:MEU1UniRule annotation
Ordered Locus Names:YLR017W
ORF Names:L1595
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR017W
SGDiS000004007 MEU1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001845561 – 337S-methyl-5'-thioadenosine phosphorylaseAdd BLAST337

Proteomic databases

MaxQBiQ07938
PaxDbiQ07938
PRIDEiQ07938

PTM databases

iPTMnetiQ07938

Interactioni

Subunit structurei

Homotrimer.UniRule annotation

Protein-protein interaction databases

BioGridi31291, 80 interactors
DIPiDIP-989N
IntActiQ07938, 2 interactors
STRINGi4932.YLR017W

Structurei

3D structure databases

ProteinModelPortaliQ07938
SMRiQ07938
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni88 – 89Phosphate bindingUniRule annotation2
Regioni121 – 122Phosphate bindingUniRule annotation2
Regioni254 – 256Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

Phylogenomic databases

GeneTreeiENSGT00550000074874
HOGENOMiHOG000228986
KOiK00772
OMAiNLYRSWG
OrthoDBiEOG092C46SL

Family and domain databases

HAMAPiMF_01963 MTAP, 1 hit
InterProiView protein in InterPro
IPR010044 MTAP
IPR000845 Nucleoside_phosphorylase_d
IPR035994 Nucleoside_phosphorylase_sf
IPR018099 Purine_phosphorylase-2_CS
PANTHERiPTHR42679 PTHR42679, 1 hit
PfamiView protein in Pfam
PF01048 PNP_UDP_1, 1 hit
SUPFAMiSSF53167 SSF53167, 1 hit
PROSITEiView protein in PROSITE
PS01240 PNP_MTAP_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q07938-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRIKNTFSV AKRLKLSKVM TNSELPSIFE GTVDLGIIGG TGLYNLDCLE
60 70 80 90 100
PIALLPPMVT PWGTTSSPVT ISQFVGTNSH FHVAFIARHG INHEYPPTKV
110 120 130 140 150
PFRANMAALK NLNCKAVLSF SAVGSLQPHI KPRDFVLPQQ IIDRTKGIRH
160 170 180 190 200
SSYFNDEGLV GHVGFGQPFS QKFAEYIYQF KNEITNPESE EPCHLHYDKD
210 220 230 240 250
MTVVCMEGPQ FSTRAESKMY RMFGGHVINM SVIPEAKLAR ECELPYQMIC
260 270 280 290 300
MSTDYDAWRD EAEPVTVETV IGNLTNNGRN ANILASKIIV SMAKEIPEFM
310 320 330
HTGDGLRGSI KKSISTKPEA MSKETLERLR YLFPNYW
Length:337
Mass (Da):37,857
Last modified:November 1, 1997 - v1
Checksum:i99B54F62ED8A9389
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti55L → V in CAA62156 (Ref. 1) Curated1
Sequence conflicti157E → G in AAT93089 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X90564 Genomic DNA Translation: CAA62156.1
Z73189 Genomic DNA Translation: CAA97539.1
AY693070 Genomic DNA Translation: AAT93089.1
BK006945 Genomic DNA Translation: DAA09335.1
PIRiS64839
RefSeqiNP_013117.1, NM_001181904.1

Genome annotation databases

EnsemblFungiiYLR017W; YLR017W; YLR017W
GeneIDi850704
KEGGisce:YLR017W

Similar proteinsi

Entry informationi

Entry nameiMTAP_YEAST
AccessioniPrimary (citable) accession number: Q07938
Secondary accession number(s): D6VY19, E9P915, Q06899
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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