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Q07938 (MTAP_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
S-methyl-5'-thioadenosine phosphorylase

EC=2.4.2.28
Alternative name(s):
5'-methylthioadenosine phosphorylase
Short name=MTA phosphorylase
Short name=MTAP
Short name=MTAPase
Multicopy enhancer of UAS2
Gene names
Name:MEU1
Ordered Locus Names:YLR017W
ORF Names:L1595
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length337 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates. Seems to be implicated in the regulation of the expression of the ADH2 gene. Ref.5 Ref.6 Ref.9 Ref.10

Catalytic activity

S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate. HAMAP-Rule MF_03155

Pathway

Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route): step 1/1. HAMAP-Rule MF_03155

Subunit structure

Homotrimer By similarity. HAMAP-Rule MF_03155

Subcellular location

Cytoplasm. Nucleus Ref.5 Ref.7.

Miscellaneous

Present with 4820 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 337337S-methyl-5'-thioadenosine phosphorylase HAMAP-Rule MF_03155
PRO_0000184556

Regions

Region88 – 892Phosphate binding By similarity
Region121 – 1222Phosphate binding By similarity
Region254 – 2563Substrate binding By similarity

Sites

Binding site411Phosphate By similarity
Binding site2301Substrate; via amide nitrogen By similarity
Binding site2311Phosphate By similarity
Site2121Important for substrate specificity By similarity
Site2671Important for substrate specificity By similarity

Experimental info

Sequence conflict551L → V in CAA62156. Ref.1
Sequence conflict1571E → G in AAT93089. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q07938 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 99B54F62ED8A9389

FASTA33737,857
        10         20         30         40         50         60 
MNRIKNTFSV AKRLKLSKVM TNSELPSIFE GTVDLGIIGG TGLYNLDCLE PIALLPPMVT 

        70         80         90        100        110        120 
PWGTTSSPVT ISQFVGTNSH FHVAFIARHG INHEYPPTKV PFRANMAALK NLNCKAVLSF 

       130        140        150        160        170        180 
SAVGSLQPHI KPRDFVLPQQ IIDRTKGIRH SSYFNDEGLV GHVGFGQPFS QKFAEYIYQF 

       190        200        210        220        230        240 
KNEITNPESE EPCHLHYDKD MTVVCMEGPQ FSTRAESKMY RMFGGHVINM SVIPEAKLAR 

       250        260        270        280        290        300 
ECELPYQMIC MSTDYDAWRD EAEPVTVETV IGNLTNNGRN ANILASKIIV SMAKEIPEFM 

       310        320        330 
HTGDGLRGSI KKSISTKPEA MSKETLERLR YLFPNYW 

« Hide

References

« Hide 'large scale' references
[1]"A 7.8kb fragment from chromosome XII of Saccharomyces cerevisiae does not harbour PKC2."
Saville S.P., Atkinson S., Jamieson L., Pocklington M.J., Orr E.
Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. expand/collapse author list , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Isolation and identification of genes activating UAS2-dependent ADH2 expression in Saccharomyces cerevisiae."
Donoviel M.S., Young E.T.
Genetics 143:1137-1148(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]"Methylthioadenosine phosphorylase regulates ornithine decarboxylase by production of downstream metabolites."
Subhi A.L., Diegelman P., Porter C.W., Tang B., Lu Z.J., Markham G.D., Kruger W.D.
J. Biol. Chem. 278:49868-49873(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"Methylthioadenosine and polyamine biosynthesis in a Saccharomyces cerevisiae meu1delta mutant."
Chattopadhyay M.K., Tabor C.W., Tabor H.
Biochem. Biophys. Res. Commun. 343:203-207(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[10]"A complete inventory of all enzymes in the eukaryotic methionine salvage pathway."
Pirkov I., Norbeck J., Gustafsson L., Albers E.
FEBS J. 275:4111-4120(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X90564 Genomic DNA. Translation: CAA62156.1.
Z73189 Genomic DNA. Translation: CAA97539.1.
AY693070 Genomic DNA. Translation: AAT93089.1.
BK006945 Genomic DNA. Translation: DAA09335.1.
PIRS64839.
RefSeqNP_013117.1. NM_001181904.1.

3D structure databases

ProteinModelPortalQ07938.
SMRQ07938. Positions 35-298.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31291. 26 interactions.
DIPDIP-989N.
IntActQ07938. 2 interactions.
MINTMINT-4494408.

Proteomic databases

PaxDbQ07938.
PeptideAtlasQ07938.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYLR017W; YLR017W; YLR017W.
GeneID850704.
KEGGsce:YLR017W.

Organism-specific databases

CYGDYLR017w.
SGDS000004007. MEU1.

Phylogenomic databases

eggNOGCOG0005.
GeneTreeENSGT00550000074874.
HOGENOMHOG000228986.
KOK00772.
OMAGCADVLK.
OrthoDBEOG77DJGM.

Enzyme and pathway databases

BioCycYEAST:G3O-32178-MONOMER.
YEAST:MONOMER3O-157.
UniPathwayUPA00904; UER00873.

Gene expression databases

GenevestigatorQ07938.

Family and domain databases

Gene3D3.40.50.1580. 1 hit.
HAMAPMF_01963. MTAP.
InterProIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERPTHR11904. PTHR11904. 1 hit.
PfamPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMSSF53167. SSF53167. 1 hit.
PROSITEPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio966748.
PROQ07938.

Entry information

Entry nameMTAP_YEAST
AccessionPrimary (citable) accession number: Q07938
Secondary accession number(s): D6VY19, E9P915, Q06899
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways