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Protein

Annexin A2

Gene

Anxa2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. Inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism but also competes with LDLR for binding with PCSK9.By similarity

GO - Molecular functioni

  • actin filament binding Source: RGD
  • calcium-dependent phospholipid binding Source: UniProtKB-KW
  • calcium ion binding Source: RGD
  • phosphatidylinositol-4,5-bisphosphate binding Source: RGD
  • phospholipase A2 inhibitor activity Source: Ensembl
  • poly(A) RNA binding Source: Ensembl
  • Rab GTPase binding Source: RGD
  • receptor activator activity Source: Ensembl

GO - Biological processi

  • angiogenesis Source: Ensembl
  • body fluid secretion Source: RGD
  • collagen fibril organization Source: Ensembl
  • fibrinolysis Source: Ensembl
  • membrane budding Source: Ensembl
  • membrane raft assembly Source: Ensembl
  • negative regulation of low-density lipoprotein particle receptor catabolic process Source: Ensembl
  • negative regulation of receptor binding Source: Ensembl
  • negative regulation of receptor internalization Source: Ensembl
  • osteoclast development Source: Ensembl
  • positive regulation of binding Source: Ensembl
  • positive regulation of fibroblast proliferation Source: RGD
  • positive regulation of protein phosphorylation Source: RGD
  • positive regulation of vesicle fusion Source: Ensembl
  • protein heterotetramerization Source: Ensembl
  • protein targeting to plasma membrane Source: RGD
  • regulation of fibrinolysis Source: RGD
  • response to thyroid hormone Source: RGD
Complete GO annotation...

Keywords - Ligandi

Calcium, Calcium/phospholipid-binding

Enzyme and pathway databases

ReactomeiR-RNO-75205. Dissolution of Fibrin Clot.

Names & Taxonomyi

Protein namesi
Recommended name:
Annexin A2
Alternative name(s):
Annexin II
Annexin-2
Calpactin I heavy chain
Calpactin-1 heavy chain
Chromobindin-8
Lipocortin II
Placental anticoagulant protein IV
Short name:
PAP-IV
Protein I
p36
Gene namesi
Name:Anxa2
Synonyms:Anx2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi621170. Anxa2.

Subcellular locationi

GO - Cellular componenti

  • basement membrane Source: UniProtKB-SubCell
  • basolateral plasma membrane Source: Ensembl
  • cell cortex Source: RGD
  • cell surface Source: Ensembl
  • cytoplasm Source: RGD
  • cytosol Source: Ensembl
  • early endosome Source: Ensembl
  • extracellular exosome Source: Ensembl
  • extracellular space Source: Ensembl
  • extrinsic component of plasma membrane Source: Ensembl
  • late endosome membrane Source: Ensembl
  • lipid particle Source: Ensembl
  • lysosomal membrane Source: Ensembl
  • macropinosome Source: RGD
  • melanosome Source: UniProtKB-SubCell
  • membrane raft Source: Ensembl
  • midbody Source: Ensembl
  • myelin sheath adaxonal region Source: Ensembl
  • nucleus Source: Ensembl
  • PCSK9-AnxA2 complex Source: Ensembl
  • perinuclear region of cytoplasm Source: RGD
  • plasma membrane Source: RGD
  • protein complex Source: RGD
  • ruffle Source: RGD
  • sarcolemma Source: Ensembl
  • Schmidt-Lanterman incisure Source: Ensembl
  • vesicle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Basement membrane, Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 339338Annexin A2PRO_0000067473Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei24 – 241Phosphotyrosine; by SRCBy similarity
Modified residuei26 – 261Phosphoserine; by PKCBy similarity
Modified residuei49 – 491N6-acetyllysineBy similarity
Modified residuei152 – 1521N6-acetyllysineBy similarity
Modified residuei199 – 1991PhosphotyrosineBy similarity
Modified residuei227 – 2271N6-acetyllysineBy similarity

Post-translational modificationi

ISGylated.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ07936.
PRIDEiQ07936.

PTM databases

iPTMnetiQ07936.
PhosphoSiteiQ07936.

Expressioni

Gene expression databases

GenevisibleiQ07936. RN.

Interactioni

Subunit structurei

Heterotetramer containing 2 light chains of S100A10/p11 and 2 heavy chains of ANXA2/p36. Interacts with ATP1B1 and DYSF (By similarity). Interacts with COCH. Interacts (via repeat Annexin 1) with PCSK9 (via the C-terminal domain); the interaction inhibits the degradation of LDLR (By similarity).By similarity

GO - Molecular functioni

  • actin filament binding Source: RGD
  • Rab GTPase binding Source: RGD

Protein-protein interaction databases

BioGridi248566. 3 interactions.
IntActiQ07936. 3 interactions.
MINTiMINT-4997127.
STRINGi10116.ENSRNOP00000038428.

Structurei

3D structure databases

ProteinModelPortaliQ07936.
SMRiQ07936. Positions 21-339.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati42 – 10261Annexin 1Add
BLAST
Repeati114 – 17461Annexin 2Add
BLAST
Repeati199 – 25961Annexin 3Add
BLAST
Repeati274 – 33461Annexin 4Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 2423S100A10-binding siteSequence analysisAdd
BLAST

Domaini

A pair of annexin repeats may form one binding site for calcium and phospholipid.

Sequence similaritiesi

Belongs to the annexin family.Curated
Contains 4 annexin repeats.Curated

Keywords - Domaini

Annexin, Repeat

Phylogenomic databases

eggNOGiKOG0819. Eukaryota.
ENOG410XPUN. LUCA.
GeneTreeiENSGT00760000118972.
HOGENOMiHOG000158803.
HOVERGENiHBG061815.
InParanoidiQ07936.
KOiK17092.
OMAiFIQQDTK.
OrthoDBiEOG74XS72.
PhylomeDBiQ07936.
TreeFamiTF105452.

Family and domain databases

Gene3Di1.10.220.10. 4 hits.
InterProiIPR001464. Annexin.
IPR018502. Annexin_repeat.
IPR018252. Annexin_repeat_CS.
IPR002389. AnnexinII.
[Graphical view]
PANTHERiPTHR10502:SF111. PTHR10502:SF111. 1 hit.
PfamiPF00191. Annexin. 4 hits.
[Graphical view]
PRINTSiPR00196. ANNEXIN.
PR00198. ANNEXINII.
SMARTiSM00335. ANX. 4 hits.
[Graphical view]
PROSITEiPS00223. ANNEXIN. 4 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Short (identifier: Q07936-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSTVHEILCK LSLEGDHSTP PSAYGSVKPY TNFDAERDAL NIETAIKTKG
60 70 80 90 100
VDEVTIVNIL TNRSNAQRQD IAFAYQRRTK KELPSAMKSA LSGHLETVML
110 120 130 140 150
GLLKTPAQYD ASELKASMKG LGTDEDSLIE IICSRTNQEL QEINRVYKEM
160 170 180 190 200
YKTDLEKDII SDTSGEFRKL LVALAKGKRA EDGSVIDYEL IDQDARELYD
210 220 230 240 250
AGVKRKGTDV PKWISIMTER SVCHLQKVFE RYKSYSPYDM LESIRKEVKG
260 270 280 290 300
DLENAFLNLV QCIQNKPLYF ADRLYDSMKG KGTRDKVLIR IMVSRSEVDM
310 320 330
LKIRSEFKRK YGKSLYYFIQ QDTKGDYQKA LLYLCGGDD
Length:339
Mass (Da):38,678
Last modified:January 23, 2007 - v2
Checksum:i101E67F50C01CF8B
GO
Isoform Long (identifier: Q07936-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     16-16: D → DSQ

Show »
Length:341
Mass (Da):38,893
Checksum:iB69F20920DAC93ED
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti103 – 1031L → F in AAB31934 (PubMed:8092993).Curated
Sequence conflicti103 – 1031L → F in AAB31933 (PubMed:8092993).Curated
Sequence conflicti322 – 3221D → Y in AAB31934 (PubMed:8092993).Curated
Sequence conflicti322 – 3221D → Y in AAB31933 (PubMed:8092993).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei16 – 161D → DSQ in isoform Long. 1 PublicationVSP_000287

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66871 mRNA. Translation: CAA47343.1.
L13039 mRNA. Translation: AAA40741.1.
S73559 mRNA. Translation: AAB31934.2.
S73557 mRNA. Translation: AAB31933.2.
BC059136 mRNA. Translation: AAH59136.1.
PIRiS33700.
S55277.
RefSeqiNP_063970.1. NM_019905.1. [Q07936-1]
UniGeneiRn.90546.

Genome annotation databases

EnsembliENSRNOT00000038677; ENSRNOP00000038428; ENSRNOG00000010362. [Q07936-1]
GeneIDi56611.
KEGGirno:56611.
UCSCiRGD:621170. rat. [Q07936-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Protein Spotlight

Red velvet - Issue 86 of September 2007

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66871 mRNA. Translation: CAA47343.1.
L13039 mRNA. Translation: AAA40741.1.
S73559 mRNA. Translation: AAB31934.2.
S73557 mRNA. Translation: AAB31933.2.
BC059136 mRNA. Translation: AAH59136.1.
PIRiS33700.
S55277.
RefSeqiNP_063970.1. NM_019905.1. [Q07936-1]
UniGeneiRn.90546.

3D structure databases

ProteinModelPortaliQ07936.
SMRiQ07936. Positions 21-339.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248566. 3 interactions.
IntActiQ07936. 3 interactions.
MINTiMINT-4997127.
STRINGi10116.ENSRNOP00000038428.

PTM databases

iPTMnetiQ07936.
PhosphoSiteiQ07936.

Proteomic databases

PaxDbiQ07936.
PRIDEiQ07936.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000038677; ENSRNOP00000038428; ENSRNOG00000010362. [Q07936-1]
GeneIDi56611.
KEGGirno:56611.
UCSCiRGD:621170. rat. [Q07936-1]

Organism-specific databases

CTDi302.
RGDi621170. Anxa2.

Phylogenomic databases

eggNOGiKOG0819. Eukaryota.
ENOG410XPUN. LUCA.
GeneTreeiENSGT00760000118972.
HOGENOMiHOG000158803.
HOVERGENiHBG061815.
InParanoidiQ07936.
KOiK17092.
OMAiFIQQDTK.
OrthoDBiEOG74XS72.
PhylomeDBiQ07936.
TreeFamiTF105452.

Enzyme and pathway databases

ReactomeiR-RNO-75205. Dissolution of Fibrin Clot.

Miscellaneous databases

PROiQ07936.

Gene expression databases

GenevisibleiQ07936. RN.

Family and domain databases

Gene3Di1.10.220.10. 4 hits.
InterProiIPR001464. Annexin.
IPR018502. Annexin_repeat.
IPR018252. Annexin_repeat_CS.
IPR002389. AnnexinII.
[Graphical view]
PANTHERiPTHR10502:SF111. PTHR10502:SF111. 1 hit.
PfamiPF00191. Annexin. 4 hits.
[Graphical view]
PRINTSiPR00196. ANNEXIN.
PR00198. ANNEXINII.
SMARTiSM00335. ANX. 4 hits.
[Graphical view]
PROSITEiPS00223. ANNEXIN. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of rat calpactin I heavy-chain cDNA whose expression is induced in v-src-transformed rat culture cell lines."
    Ozaki T., Sakiyama S.
    Oncogene 8:1707-1710(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
  2. Ernst J.D.
    Submitted (MAR-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
  3. "Molecular cloning of a novel N-terminal variant of annexin II from rat basophilic leukaemia cells."
    Upton A.L., Moss S.E.
    Biochem. J. 302:425-428(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SHORT).
    Tissue: Pituitary.
  5. Bienvenut W.V., von Kriegsheim A., Kolch W.
    Submitted (JUN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-10; 38-47; 50-63; 69-77; 89-115; 120-145; 158-168; 180-204; 234-245 AND 314-324, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Fibroblast.
  6. Lubec G., Afjehi-Sadat L., Kang S.U.
    Submitted (JUL-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 38-47; 50-63; 89-115; 136-145; 158-168; 180-196 AND 314-324 (ISOFORMS SHORT/LONG), IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain and Spinal cord.
  7. "CAP-50, a newly identified annexin, localizes in nuclei of cultured fibroblast 3Y1 cells."
    Mizutani A., Usuda N., Tokumitsu H., Minami H., Yasui K., Kobayashi R., Hidaka H.
    J. Biol. Chem. 267:13498-13504(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  8. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiANXA2_RAT
AccessioniPrimary (citable) accession number: Q07936
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 147 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

It may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.