Q07936 (ANXA2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Annexin A2

Alternative name(s):
Annexin II
Annexin-2
Calpactin I heavy chain
Calpactin-1 heavy chain
Chromobindin-8
Lipocortin II
Placental anticoagulant protein IV
Short name=PAP-IV
Protein I
p36

Gene names

Name:	Anxa2
Synonyms:	Anx2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity.
Subunit structure	Heterotetramer containing 2 light chains of S100A10/p11 and 2 heavy chains of ANXA2/p36. Interacts with ATP1B1 and DYSF By similarity. Interacts with COCH By similarity.
Subcellular location	Secreted › extracellular space › extracellular matrix › basement membrane. Melanosome By similarity. Note: In the lamina beneath the plasma membrane. Ref.7
Domain	A pair of annexin repeats may form one binding site for calcium and phospholipid.
Post-translational modification	ISGylated By similarity.
Miscellaneous	It may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis.
Sequence similarities	Belongs to the annexin family. Contains 4 annexin repeats.

Ontologies

Keywords
Cellular component	Basement membrane Extracellular matrix Secreted
Coding sequence diversity	Alternative splicing
Domain	Annexin Repeat
Ligand	Calcium Calcium/phospholipid-binding
PTM	Acetylation Phosphoprotein Ubl conjugation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	angiogenesis Inferred from electronic annotation. Source: Compara body fluid secretion Inferred from mutant phenotype PubMed 18332131. Source: RGD cellular response to acid Inferred from electronic annotation. Source: Compara collagen fibril organization Inferred from electronic annotation. Source: Compara fibrinolysis Inferred from electronic annotation. Source: Compara positive regulation of binding Inferred from electronic annotation. Source: Compara positive regulation of vesicle fusion Inferred from electronic annotation. Source: Compara regulation of fibrinolysis Traceable author statement PubMed 11739291. Source: RGD
Cellular_component	Schmidt-Lanterman incisure Inferred from electronic annotation. Source: Compara basement membrane Inferred from electronic annotation. Source: UniProtKB-SubCell early endosome Inferred from electronic annotation. Source: Compara extrinsic to plasma membrane Inferred from electronic annotation. Source: Compara lipid particle Inferred from electronic annotation. Source: Compara melanosome Inferred from electronic annotation. Source: UniProtKB-SubCell midbody Inferred from electronic annotation. Source: Compara myelin sheath adaxonal region Inferred from electronic annotation. Source: Compara perinuclear region of cytoplasm Inferred from direct assay PubMed 14734570. Source: RGD plasma membrane Inferred from direct assay PubMed 14734570. Source: RGD protein complex Inferred from direct assay PubMed 15823548. Source: RGD sarcolemma Inferred from electronic annotation. Source: Compara
Molecular_function	actin filament binding Traceable author statement PubMed 14734570. Source: RGD calcium ion binding Traceable author statement PubMed 14734570. Source: RGD calcium-dependent phospholipid binding Inferred from electronic annotation. Source: UniProtKB-KW phosphatidylinositol-4,5-bisphosphate binding Inferred from direct assay PubMed 14734570. Source: RGD phospholipase inhibitor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Short (identifier: Q07936-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Long (identifier: Q07936-2)

The sequence of this isoform differs from the canonical sequence as follows:
     16-16: D → DSQ

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.5
	Chain	2 – 339	338	Annexin A2		PRO_0000067473
Regions
	Repeat	42 – 102	61	Annexin 1
	Repeat	114 – 174	61	Annexin 2
	Repeat	199 – 259	61	Annexin 3
	Repeat	274 – 334	61	Annexin 4
	Region	2 – 24	23	S100A10-binding site Potential
Amino acid modifications
	Modified residue	2	1	N-acetylserine Ref.5
	Modified residue	24	1	Phosphotyrosine; by SRC By similarity
	Modified residue	26	1	Phosphoserine; by PKC By similarity
Natural variations
	Alternative sequence	16	1	D → DSQ in isoform Long.		VSP_000287
Experimental info
	Sequence conflict	103	1	L → F in AAB31934. Ref.3
	Sequence conflict	103	1	L → F in AAB31933. Ref.3
	Sequence conflict	322	1	D → Y in AAB31934. Ref.3
	Sequence conflict	322	1	D → Y in AAB31933. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform Short [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 101E67F50C01CF8B	FASTA	339	38,678
	10 20 30 40 50 60 MSTVHEILCK LSLEGDHSTP PSAYGSVKPY TNFDAERDAL NIETAIKTKG VDEVTIVNIL 70 80 90 100 110 120 TNRSNAQRQD IAFAYQRRTK KELPSAMKSA LSGHLETVML GLLKTPAQYD ASELKASMKG 130 140 150 160 170 180 LGTDEDSLIE IICSRTNQEL QEINRVYKEM YKTDLEKDII SDTSGEFRKL LVALAKGKRA 190 200 210 220 230 240 EDGSVIDYEL IDQDARELYD AGVKRKGTDV PKWISIMTER SVCHLQKVFE RYKSYSPYDM 250 260 270 280 290 300 LESIRKEVKG DLENAFLNLV QCIQNKPLYF ADRLYDSMKG KGTRDKVLIR IMVSRSEVDM 310 320 330 LKIRSEFKRK YGKSLYYFIQ QDTKGDYQKA LLYLCGGDD
	Isoform Long [UniParc]. Checksum: B69F20920DAC93ED	FASTA	341	38,893
	10 20 30 40 50 60 MSTVHEILCK LSLEGDSQHS TPPSAYGSVK PYTNFDAERD ALNIETAIKT KGVDEVTIVN 70 80 90 100 110 120 ILTNRSNAQR QDIAFAYQRR TKKELPSAMK SALSGHLETV MLGLLKTPAQ YDASELKASM 130 140 150 160 170 180 KGLGTDEDSL IEIICSRTNQ ELQEINRVYK EMYKTDLEKD IISDTSGEFR KLLVALAKGK 190 200 210 220 230 240 RAEDGSVIDY ELIDQDAREL YDAGVKRKGT DVPKWISIMT ERSVCHLQKV FERYKSYSPY 250 260 270 280 290 300 DMLESIRKEV KGDLENAFLN LVQCIQNKPL YFADRLYDSM KGKGTRDKVL IRIMVSRSEV 310 320 330 340 DMLKIRSEFK RKYGKSLYYF IQQDTKGDYQ KALLYLCGGD D

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of rat calpactin I heavy-chain cDNA whose expression is induced in v-src-transformed rat culture cell lines." Ozaki T., Sakiyama S. Oncogene 8:1707-1710(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
[2]	Ernst J.D. Submitted (MAR-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
[3]	"Molecular cloning of a novel N-terminal variant of annexin II from rat basophilic leukaemia cells." Upton A.L., Moss S.E. Biochem. J. 302:425-428(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SHORT). Tissue: Pituitary.
[5]	Bienvenut W.V., von Kriegsheim A., Kolch W. Submitted (JUN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-10; 38-47; 50-63; 69-77; 89-115; 120-145; 158-168; 180-204; 234-245 AND 314-324, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2, MASS SPECTROMETRY. Tissue: Fibroblast.
[6]	Lubec G., Afjehi-Sadat L., Kang S.U. Submitted (JUL-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 38-47; 50-63; 89-115; 136-145; 158-168; 180-196 AND 314-324 (ISOFORMS SHORT/LONG), MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain and Spinal cord.
[7]	"CAP-50, a newly identified annexin, localizes in nuclei of cultured fibroblast 3Y1 cells." Mizutani A., Usuda N., Tokumitsu H., Minami H., Yasui K., Kobayashi R., Hidaka H. J. Biol. Chem. 267:13498-13504(1992) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Web resources

Protein Spotlight

Red velvet - Issue 86 of September 2007

Cross-references

Sequence databases
EMBL GenBank DDBJ	X66871 mRNA. Translation: CAA47343.1. L13039 mRNA. Translation: AAA40741.1. S73559 mRNA. Translation: AAB31934.2. S73557 mRNA. Translation: AAB31933.2. BC059136 mRNA. Translation: AAH59136.1.
IPI	IPI00325146. IPI00829462.
PIR	S33700. S55277.
RefSeq	NP_063970.1. NM_019905.1.
UniGene	Rn.90546.
3D structure databases
ProteinModelPortal	Q07936.
SMR	Q07936. Positions 21-339.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q07936. 3 interactions.
STRING	10116.ENSRNOP00000038428.
PTM databases
PhosphoSite	Q07936.
Proteomic databases
PaxDb	Q07936.
PRIDE	Q07936.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000038677; ENSRNOP00000038428; ENSRNOG00000010362.
GeneID	56611.
KEGG	rno:56611.
UCSC	RGD:621170. rat.
Organism-specific databases
CTD	302.
RGD	621170. Anxa2.
Phylogenomic databases
eggNOG	NOG259189.
GeneTree	ENSGT00680000099735.
HOGENOM	HOG000158803.
HOVERGEN	HBG061815.
OrthoDB	EOG4KD6MM.
Gene expression databases
Genevestigator	Q07936.
GermOnline	ENSRNOG00000010362. Rattus norvegicus.
Family and domain databases
Gene3D	1.10.220.10. 4 hits.
InterPro	IPR001464. Annexin. IPR018502. Annexin_repeat. IPR018252. Annexin_repeat_CS. IPR002389. AnnexinII. [Graphical view]
PANTHER	PTHR10502. PTHR10502. 1 hit. PTHR10502:SF18. PTHR10502:SF18. 1 hit.
Pfam	PF00191. Annexin. 4 hits. [Graphical view]
PRINTS	PR00196. ANNEXIN. PR00198. ANNEXINII.
SMART	SM00335. ANX. 4 hits. [Graphical view]
SUPFAM	SSF47874. Annexin. 1 hit.
PROSITE	PS00223. ANNEXIN. 4 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	611122.

Entry information

Entry name

ANXA2_RAT

Accession

Primary (citable) accession number: Q07936

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 122 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program