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Protein

Pilosulin-1

Gene
N/A
Organism
Myrmecia pilosula (Jack jumper ant) (Australian jumper ant)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has strong cytotoxic and hemolytic activities. Is more potent against mononuclear leukocytes than against granulocytes. The synthesized peptide 57-76 shows a potent and broad spectrum antimicrobial activity against both Gram-positive and Gram-negative bacteria, and also against the fungus C.albicans. Adopts an alpha-helical structure.3 Publications

GO - Biological processi

  • cytolysis in other organism Source: CAFA
  • defense response to fungus Source: UniProtKB-KW
  • defense response to Gram-negative bacterium Source: CAFA
  • defense response to Gram-positive bacterium Source: CAFA
  • hemolysis in other organism Source: CAFA

Keywordsi

Molecular functionAntibiotic, Antimicrobial, Fungicide
Biological processCytolysis, Hemolysis

Protein family/group databases

TCDBi1.C.51.1.1. the pilosulin (pilosulin) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Pilosulin-1
Alternative name(s):
Allergen Myr p I
Major allergen Myr p 1
Allergen: Myr p 1
Cleaved into the following 5 chains:
OrganismiMyrmecia pilosula (Jack jumper ant) (Australian jumper ant)
Taxonomic identifieri13618 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaHymenopteraApocritaAculeataVespoideaFormicidaeMyrmeciinaeMyrmeciiniMyrmecia

Subcellular locationi

GO - Cellular componenti

  • extracellular space Source: CAFA
  • other organism cell membrane Source: CAFA

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. Binds to IgE.

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei3380. Myr p 1.0101.
480. Myr p 1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26Sequence analysisAdd BLAST26
PropeptideiPRO_000003515827 – 562 PublicationsAdd BLAST30
PeptideiPRO_000003515957 – 112Pilosulin-1Add BLAST56
PeptideiPRO_000003516065 – 112Pilosulin-1 65->112Add BLAST48
PeptideiPRO_000003516168 – 112Pilosulin-1 68->112Add BLAST45
PeptideiPRO_000003516271 – 112Pilosulin-1 71->112Add BLAST42
PeptideiPRO_000003516386 – 112Pilosulin-1 86->112Add BLAST27

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

Secondary structure

1112
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi62 – 68Combined sources7
Turni69 – 72Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5X3LNMR-A57-76[»]
DisProtiDP00567.
SMRiQ07932.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni57 – 78Critical for cytotoxic activityAdd BLAST22
Regioni93 – 106IgE-binding determinantAdd BLAST14

Sequence similaritiesi

Belongs to the pilosulin family.Curated

Keywords - Domaini

Signal

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q07932-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLSCLLLTL TIIFVLTIVH APNVEAKDLA DPESEAVGFA DAFGEADAVG
60 70 80 90 100
EADPNAGLGS VFGRLARILG RVIPKVAKKL GPKVAKVLPK VMKEAIPMAV
110
EMAKSQEEQQ PQ
Length:112
Mass (Da):11,849
Last modified:February 1, 1995 - v1
Checksum:iF1F90510A82ED4EF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti82 – 83PK → KT AA sequence (PubMed:8605256).Curated2

Mass spectrometryi

Molecular mass is 4938 Da from positions 68 - 112. Determined by ESI. 1 Publication
Molecular mass is 5279 Da from positions 65 - 112. Determined by ESI. 1 Publication
Molecular mass is 6052 Da from positions 57 - 112. Determined by ESI. 1 Publication
Molecular mass is 6067 Da from positions 57 - 112. Determined by ESI. Variant Ile-61.1 Publication

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti61V → I. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70256 mRNA. Translation: CAA49760.1.
PIRiS28180.
S65709.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiMYR1_MYRPI
AccessioniPrimary (citable) accession number: Q07932
Secondary accession number(s): Q9TWC1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 10, 2017
This is version 71 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families