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Protein

Pilosulin-1

Gene
N/A
Organism
Myrmecia pilosula (Jack jumper ant) (Australian jumper ant)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Has strong cytotoxic and hemolytic activities. Is more potent against mononuclear leukocytes than against granulocytes. The synthesized peptide 57-76 shows a potent and broad spectrum antimicrobial activity against both Gram-positive and Gram-negative bacteria, and also against the fungus C.albicans. Adopts an alpha-helical structure.3 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Fungicide

Keywords - Biological processi

Cytolysis, Hemolysis

Protein family/group databases

TCDBi1.C.51.1.1. the pilosulin (pilosulin) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Pilosulin-1
Alternative name(s):
Allergen Myr p I
Major allergen Myr p 1
Allergen: Myr p 1
Cleaved into the following 5 chains:
OrganismiMyrmecia pilosula (Jack jumper ant) (Australian jumper ant)
Taxonomic identifieri13618 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaHymenopteraApocritaAculeataVespoideaFormicidaeMyrmeciinaeMyrmeciiniMyrmecia

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. Binds to IgE.

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei3380. Myr p 1.0101.
480. Myr p 1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence analysisAdd
BLAST
Propeptidei27 – 56302 PublicationsPRO_0000035158Add
BLAST
Peptidei57 – 11256Pilosulin-1PRO_0000035159Add
BLAST
Peptidei65 – 11248Pilosulin-1 65->112PRO_0000035160Add
BLAST
Peptidei68 – 11245Pilosulin-1 68->112PRO_0000035161Add
BLAST
Peptidei71 – 11242Pilosulin-1 71->112PRO_0000035162Add
BLAST
Peptidei86 – 11227Pilosulin-1 86->112PRO_0000035163Add
BLAST

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

3D structure databases

DisProtiDP00567.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni57 – 7822Critical for cytotoxic activityAdd
BLAST
Regioni93 – 10614IgE-binding determinantAdd
BLAST

Sequence similaritiesi

Belongs to the pilosulin family.Curated

Keywords - Domaini

Signal

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q07932-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLSCLLLTL TIIFVLTIVH APNVEAKDLA DPESEAVGFA DAFGEADAVG
60 70 80 90 100
EADPNAGLGS VFGRLARILG RVIPKVAKKL GPKVAKVLPK VMKEAIPMAV
110
EMAKSQEEQQ PQ
Length:112
Mass (Da):11,849
Last modified:February 1, 1995 - v1
Checksum:iF1F90510A82ED4EF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti82 – 832PK → KT AA sequence (PubMed:8605256).Curated

Mass spectrometryi

Molecular mass is 4938 Da from positions 68 - 112. Determined by ESI. 1 Publication
Molecular mass is 5279 Da from positions 65 - 112. Determined by ESI. 1 Publication
Molecular mass is 6052 Da from positions 57 - 112. Determined by ESI. 1 Publication
Molecular mass is 6067 Da from positions 57 - 112. Determined by ESI. Variant Ile-61.1 Publication

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti61 – 611V → I.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70256 mRNA. Translation: CAA49760.1.
PIRiS28180.
S65709.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70256 mRNA. Translation: CAA49760.1.
PIRiS28180.
S65709.

3D structure databases

DisProtiDP00567.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei3380. Myr p 1.0101.
480. Myr p 1.
TCDBi1.C.51.1.1. the pilosulin (pilosulin) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and characterization of a major allergen (Myr p I) from the venom of the Australian jumper ant, Myrmecia pilosula."
    Donovan G.R., Baldo B.A., Sutherland S.K.
    Biochim. Biophys. Acta 1171:272-280(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Venom gland.
  2. "Expression of jumper ant (Myrmecia pilosula) venom allergens: post-translational processing of allergen gene products."
    Donovan G.R., Street M.D., Tetaz T., Smith A.I., Alewood D., Alewood P.F., Sutherland S.K., Baldo B.A.
    Biochem. Mol. Biol. Int. 39:877-885(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 57-112; 65-112; 68-112; 71-112 AND 86-112.
    Tissue: Venom.
  3. "Characterisation of major peptides in 'jack jumper' ant venom by mass spectrometry."
    Davies N.W., Wiese M.D., Brown S.G.A.
    Toxicon 43:173-183(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 57-66 (VARIANT), MASS SPECTROMETRY.
    Tissue: Venom.
  4. "Molecular cloning and characterization of the major allergen Myr p II from the venom of the jumper ant Myrmecia pilosula: Myr p I and Myr p II share a common protein leader sequence."
    Street M.D., Donovan G.R., Baldo B.A.
    Biochim. Biophys. Acta 1305:87-97(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 68-83.
    Tissue: Venom.
  5. "Cytotoxicity of pilosulin 1, a peptide from the venom of the jumper ant Myrmecia pilosula."
    Wu Q.-X., King M.A., Donovan G.R., Alewood D., Alewood P.F., Sawyer W.H., Baldo B.A.
    Biochim. Biophys. Acta 1425:74-80(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SYNTHESIS OF 57-78; 57-112; 67-112; 79-112; 93-112 AND 103-112.
  6. "Flow cytometric analysis of cell killing by the jumper ant venom peptide pilosulin 1."
    King M.A., Wu Q.-X., Donovan G.R., Baldo B.A.
    Cytometry 32:268-273(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SYNTHESIS OF 57-112.
  7. "Identification and optimization of an antimicrobial peptide from the ant venom toxin pilosulin."
    Zelezetsky I., Pag U., Antcheva N., Sahl H.-G., Tossi A.
    Arch. Biochem. Biophys. 434:358-364(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SYNTHESIS OF 57-76.
  8. "Identification of an IgE-binding determinant of the major allergen Myr p I from the venom of the Australian jumper ant Myrmecia pilosula."
    Donovan G.R., Street M.D., Baldo B.A., Alewood D., Alewood P.F., Sutherland S.K.
    Biochim. Biophys. Acta 1204:48-52(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: SYNTHESIS OF 67-112; 79-112; 87-112; 93-112; 95-112; 97-112; 99-112 101-112; 93-100; 93-102; 93-104 AND 93-106, IGE-BINDING DETERMINANT.

Entry informationi

Entry nameiMYR1_MYRPI
AccessioniPrimary (citable) accession number: Q07932
Secondary accession number(s): Q9TWC1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: January 7, 2015
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.