Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pre-mRNA leakage protein 1

Gene

PML1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for efficient splicing and pre-mRNA nuclear retention.1 Publication

GO - Biological processi

  • maintenance of RNA location Source: SGD
  • mRNA export from nucleus Source: SGD
  • mRNA splicing, via spliceosome Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Enzyme and pathway databases

BioCyciYEAST:G3O-32177-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA leakage protein 1
Gene namesi
Name:PML1
Ordered Locus Names:YLR016C
ORF Names:L1591
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR016C.
SGDiS000004006. PML1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • RES complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000584661 – 204Pre-mRNA leakage protein 1Add BLAST204

Proteomic databases

MaxQBiQ07930.
PRIDEiQ07930.

Interactioni

Subunit structurei

Belongs to the pre-mRNA retention and splicing (RES) complex composed of at least BUD13, IST3 and PML1.

Protein-protein interaction databases

BioGridi31290. 51 interactors.
DIPiDIP-2149N.
IntActiQ07930. 2 interactors.
MINTiMINT-556104.

Structurei

Secondary structure

1204
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi38 – 41Combined sources4
Helixi61 – 67Combined sources7
Helixi72 – 74Combined sources3
Beta strandi78 – 84Combined sources7
Helixi85 – 90Combined sources6
Beta strandi93 – 97Combined sources5
Beta strandi102 – 108Combined sources7
Beta strandi128 – 131Combined sources4
Beta strandi141 – 148Combined sources8
Beta strandi151 – 158Combined sources8
Beta strandi178 – 180Combined sources3
Beta strandi184 – 190Combined sources7
Helixi192 – 194Combined sources3
Beta strandi197 – 203Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JKDX-ray2.50A/B25-204[»]
2MKCNMR-B22-42[»]
2MY3NMR-B22-42[»]
3ELSX-ray1.80A51-204[»]
3ELVX-ray2.40A/B1-204[»]
5GM6electron microscopy3.50U1-204[»]
5LQWelectron microscopy5.80N1-204[»]
ProteinModelPortaliQ07930.
SMRiQ07930.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ07930.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini104 – 172FHAPROSITE-ProRule annotationAdd BLAST69

Sequence similaritiesi

Contains 1 FHA domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000001108.
InParanoidiQ07930.
KOiK13108.
OMAiESNNKEG.
OrthoDBiEOG092C4XK0.

Family and domain databases

Gene3Di2.60.200.20. 1 hit.
InterProiIPR000253. FHA_dom.
IPR008984. SMAD_FHA_domain.
[Graphical view]
PfamiPF00498. FHA. 1 hit.
[Graphical view]
SMARTiSM00240. FHA. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
PROSITEiPS50006. FHA_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q07930-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFHRRKRPYN TRNYGHDDKK FKSQYIDIMP DFSPSGLLEL ESNNKEGIAL
60 70 80 90 100
KHVEPQDAIS PDNYMDMLGL EARDRTMYEL VIYRKNDKDK GPWKRYDLNG
110 120 130 140 150
RSCYLVGREL GHSLDTDLDD RTEIVVADIG IPEETSSKQH CVIQFRNVRG
160 170 180 190 200
ILKCYVMDLD SSNGTCLNNV VIPGARYIEL RSGDVLTLSE FEEDNDYELI

FMNV
Length:204
Mass (Da):23,654
Last modified:November 1, 1996 - v1
Checksum:iDA39B4465EFFD378
GO

Sequence cautioni

The sequence CAA62157 differs from that shown. Reason: Frameshift at position 180.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti15G → A in CAA62157 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X90564 Genomic DNA. Translation: CAA62157.1. Frameshift.
Z73188 Genomic DNA. Translation: CAA97538.1.
AY557934 Genomic DNA. Translation: AAS56260.1.
BK006945 Genomic DNA. Translation: DAA09334.1.
PIRiS64838.
RefSeqiNP_013116.1. NM_001181903.1.

Genome annotation databases

EnsemblFungiiYLR016C; YLR016C; YLR016C.
GeneIDi850703.
KEGGisce:YLR016C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X90564 Genomic DNA. Translation: CAA62157.1. Frameshift.
Z73188 Genomic DNA. Translation: CAA97538.1.
AY557934 Genomic DNA. Translation: AAS56260.1.
BK006945 Genomic DNA. Translation: DAA09334.1.
PIRiS64838.
RefSeqiNP_013116.1. NM_001181903.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JKDX-ray2.50A/B25-204[»]
2MKCNMR-B22-42[»]
2MY3NMR-B22-42[»]
3ELSX-ray1.80A51-204[»]
3ELVX-ray2.40A/B1-204[»]
5GM6electron microscopy3.50U1-204[»]
5LQWelectron microscopy5.80N1-204[»]
ProteinModelPortaliQ07930.
SMRiQ07930.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31290. 51 interactors.
DIPiDIP-2149N.
IntActiQ07930. 2 interactors.
MINTiMINT-556104.

Proteomic databases

MaxQBiQ07930.
PRIDEiQ07930.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR016C; YLR016C; YLR016C.
GeneIDi850703.
KEGGisce:YLR016C.

Organism-specific databases

EuPathDBiFungiDB:YLR016C.
SGDiS000004006. PML1.

Phylogenomic databases

HOGENOMiHOG000001108.
InParanoidiQ07930.
KOiK13108.
OMAiESNNKEG.
OrthoDBiEOG092C4XK0.

Enzyme and pathway databases

BioCyciYEAST:G3O-32177-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ07930.
PROiQ07930.

Family and domain databases

Gene3Di2.60.200.20. 1 hit.
InterProiIPR000253. FHA_dom.
IPR008984. SMAD_FHA_domain.
[Graphical view]
PfamiPF00498. FHA. 1 hit.
[Graphical view]
SMARTiSM00240. FHA. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
PROSITEiPS50006. FHA_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPML1_YEAST
AccessioniPrimary (citable) accession number: Q07930
Secondary accession number(s): D6VY18, Q06901
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1470 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.