Reviewed, UniProtKB/Swiss-Prot Q07923 (LOT6_YEAST)

Names and origin

Protein names

Recommended name:
NAD(P)H-dependent FMN reductase LOT6
Short name=FMN reductase LOT6
EC=1.5.1.29

Alternative name(s):
Azoreductase LOT6
Low temperature response protein 6

Gene names

Name:	LOT6
Ordered Locus Names:	YLR011W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	191 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Has several reductase activities that are NAD(P)H-dependent and involve FMN as a cofactor, ferricyanide being the best substrate for reduction. May be involved in ferric iron assimilation. Ref.6
Catalytic activity	FMNH2 + NAD(P)+ = FMN + NAD(P)H.
Subunit structure	Homodimer. Ref.6
Subcellular location	Cytoplasm. Nucleus Ref.4.
Induction	Induced by low temperature and by cycloheximide. Ref.3
Miscellaneous	Present with 1270 molecules/cell in log phase SD medium. Ref.5

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Ligand	FMN Flavoprotein NAD NADP
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	cytoplasmic sequestering of transcription factor Inferred from direct assay. Source: SGD oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to oxidative stress Inferred from mutant phenotype. Source: SGD
Cellular component	cytosol Inferred from direct assay. Source: SGD nucleus Ref.4 Inferred from direct assay. Source: SGD
Molecular function	FMN reductase activity Inferred from electronic annotation. Source: EC NAD(P)H dehydrogenase (quinone) activity Inferred from direct assay. Source: SGD transcription factor binding Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	P38800	1	EBI-31608,EBI-24597

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 191

191

NAD(P)H-dependent FMN reductase LOT6

PRO_0000234661

Regions

Region

94 – 97

4

FMN binding

Sites

Binding site

11

1

FMN

Binding site

124

1

FMN

Secondary structure

1

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191

Helix Strand Turn

Details...

Beta strand

2 – 7

6

Helix

16 – 28

13

Turn

31 – 37

7

Beta strand

39 – 43

5

Helix

45 – 48

4

Helix

61 – 63

3

Helix

67 – 69

3

Helix

73 – 83

11

Beta strand

86 – 93

8

Helix

101 – 108

8

Turn

112 – 116

5

Beta strand

118 – 125

8

Turn

126 – 129

4

Helix

130 – 142

13

Beta strand

146 – 154

9

Turn

164 – 166

3

Helix

168 – 173

6

Helix

174 – 184

11

Sequences

	Sequence		Length	Mass (Da)
	Q07923-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 90C8FD4A39BA2FB7	FASTA	191	21,281
	10 20 30 40 50 60 MKVGIIMGSV RAKRVCPEIA AYVKRTIENS EELIDQKLKI QVVDLQQIAL PLYEDDDELI 70 80 90 100 110 120 PAQIKSVDEY ADSKTRSWSR IVNALDIIVF VTPQYNWGYP AALKNAIDRL YHEWHGKPAL 130 140 150 160 170 180 VVSYGGHGGS KCNDQLQEVL HGLKMNVIGG VAVKIPVGTI PLPEDIVPQL SVHNEEILQL 190 LASCIETTRN K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Multiple mechanisms regulate expression of low temperature responsive (LOT) genes in Saccharomyces cerevisiae." Zhang L., Ohta A., Horiuchi H., Takagi M., Imai R. Biochem. Biophys. Res. Commun. 283:531-535(2001) [PubMed: 11327734] [Abstract] Cited for: INDUCTION.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Crystal structure and functional characterization of yeast YLR011wp, an enzyme with NAD(P)H-FMN and ferric iron reductase activities." Liger D., Graille M., Zhou C.-Z., Leulliot N., Quevillon-Cheruel S., Blondeau K., Janin J., van Tilbeurgh H. J. Biol. Chem. 279:34890-34897(2004) [PubMed: 15184374] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS), FUNCTION, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z73183 Genomic DNA. Translation: CAA97533.1.
AY558199 Genomic DNA. Translation: AAS56525.1.

PIR

S64833.

RefSeq

NP_013111.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1T0I	X-ray	2.00	A/B	1-191	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-4604N.

IntAct

Q07923. 4 interactions.

STRING

Q07923.

Proteomic databases

PRIDE

Q07923.

Genome annotation databases

Ensembl

YLR011W; YLR011W; YLR011W; Saccharomyces cerevisiae. [Genome view]

GeneID

850698.

GenomeReviews

Gene locus YLR011W in contig Y13138_GR.

KEGG

sce:YLR011W.

NMPDR

fig|4932.3.peg.4102.

Organism-specific databases

CYGD

YLR011w.

SGD

S000004001. LOT6.

Phylogenomic databases

eggNOG

fuNOG11552.

HOGENOM

HBG697130.

OMA

TPQYNWG.

OrthoDB

EOG9NCNWJ.

PhylomeDB

Q07923.

Enzyme and pathway databases

BRENDA

1.5.1.29. 250.

Gene expression databases

ArrayExpress

Q07923.

Genevestigator

Q07923.

GermOnline

YLR011W. Saccharomyces cerevisiae.

Family and domain databases

ProtoNet

Search...

Other Resources

NextBio

966730.

Entry information

Entry name

LOT6_YEAST

Accession

Primary (citable) accession number: Q07923

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	November 1, 1996
Last modified:	March 2, 2010
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)