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Protein

NAD(P)H-dependent FMN reductase LOT6

Gene

LOT6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has several reductase activities that are NAD(P)H-dependent and involve FMN as a cofactor, ferricyanide being the best substrate for reduction. May be involved in ferric iron assimilation.1 Publication

Catalytic activityi

FMNH2 + NAD(P)+ = FMN + NAD(P)H.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei11FMN1
Binding sitei124FMN1

GO - Molecular functioni

  • FMN binding Source: GO_Central
  • FMN reductase (NADH) activity Source: UniProtKB-EC
  • FMN reductase (NADPH) activity Source: GO_Central
  • NAD(P)H dehydrogenase (quinone) activity Source: SGD
  • transcription factor binding Source: SGD

GO - Biological processi

  • apoptotic process Source: SGD
  • cellular response to oxidative stress Source: SGD
  • cytoplasmic sequestering of transcription factor Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Flavoprotein, FMN, NAD, NADP

Enzyme and pathway databases

BioCyciYEAST:G3O-32172-MONOMER.
BRENDAi1.6.5.2. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD(P)H-dependent FMN reductase LOT6 (EC:1.5.1.39)
Short name:
FMN reductase LOT6
Alternative name(s):
Azoreductase LOT6
FMN reductase [NAD(P)H]
Low temperature response protein 6
Gene namesi
Name:LOT6
Ordered Locus Names:YLR011W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR011W.
SGDiS000004001. LOT6.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002346611 – 191NAD(P)H-dependent FMN reductase LOT6Add BLAST191

Proteomic databases

MaxQBiQ07923.
PRIDEiQ07923.

Expressioni

Inductioni

Induced by low temperature and by cycloheximide.1 Publication

Interactioni

Subunit structurei

Homodimer.1 Publication

GO - Molecular functioni

  • transcription factor binding Source: SGD

Protein-protein interaction databases

BioGridi31285. 17 interactors.
DIPiDIP-4604N.
IntActiQ07923. 1 interactor.
MINTiMINT-568995.

Structurei

Secondary structure

1191
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 7Combined sources6
Helixi16 – 28Combined sources13
Turni31 – 37Combined sources7
Beta strandi39 – 43Combined sources5
Helixi45 – 48Combined sources4
Helixi61 – 63Combined sources3
Helixi67 – 69Combined sources3
Helixi73 – 83Combined sources11
Beta strandi86 – 93Combined sources8
Helixi101 – 108Combined sources8
Turni112 – 116Combined sources5
Beta strandi118 – 125Combined sources8
Turni126 – 129Combined sources4
Helixi130 – 142Combined sources13
Beta strandi146 – 154Combined sources9
Turni164 – 166Combined sources3
Helixi168 – 173Combined sources6
Helixi174 – 184Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1T0IX-ray2.00A/B1-191[»]
ProteinModelPortaliQ07923.
SMRiQ07923.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ07923.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni94 – 97FMN binding4

Phylogenomic databases

HOGENOMiHOG000263121.
InParanoidiQ07923.
OMAiSAVHIAW.
OrthoDBiEOG092C5LKY.

Family and domain databases

Gene3Di3.40.50.360. 1 hit.
InterProiIPR029039. Flavoprotein-like_dom.
IPR005025. FMN_Rdtase-like.
[Graphical view]
PfamiPF03358. FMN_red. 1 hit.
[Graphical view]
SUPFAMiSSF52218. SSF52218. 1 hit.

Sequencei

Sequence statusi: Complete.

Q07923-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVGIIMGSV RAKRVCPEIA AYVKRTIENS EELIDQKLKI QVVDLQQIAL
60 70 80 90 100
PLYEDDDELI PAQIKSVDEY ADSKTRSWSR IVNALDIIVF VTPQYNWGYP
110 120 130 140 150
AALKNAIDRL YHEWHGKPAL VVSYGGHGGS KCNDQLQEVL HGLKMNVIGG
160 170 180 190
VAVKIPVGTI PLPEDIVPQL SVHNEEILQL LASCIETTRN K
Length:191
Mass (Da):21,281
Last modified:November 1, 1996 - v1
Checksum:i90C8FD4A39BA2FB7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73183 Genomic DNA. Translation: CAA97533.1.
AY558199 Genomic DNA. Translation: AAS56525.1.
BK006945 Genomic DNA. Translation: DAA09329.1.
PIRiS64833.
RefSeqiNP_013111.1. NM_001181898.1.

Genome annotation databases

EnsemblFungiiYLR011W; YLR011W; YLR011W.
GeneIDi850698.
KEGGisce:YLR011W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73183 Genomic DNA. Translation: CAA97533.1.
AY558199 Genomic DNA. Translation: AAS56525.1.
BK006945 Genomic DNA. Translation: DAA09329.1.
PIRiS64833.
RefSeqiNP_013111.1. NM_001181898.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1T0IX-ray2.00A/B1-191[»]
ProteinModelPortaliQ07923.
SMRiQ07923.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31285. 17 interactors.
DIPiDIP-4604N.
IntActiQ07923. 1 interactor.
MINTiMINT-568995.

Proteomic databases

MaxQBiQ07923.
PRIDEiQ07923.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR011W; YLR011W; YLR011W.
GeneIDi850698.
KEGGisce:YLR011W.

Organism-specific databases

EuPathDBiFungiDB:YLR011W.
SGDiS000004001. LOT6.

Phylogenomic databases

HOGENOMiHOG000263121.
InParanoidiQ07923.
OMAiSAVHIAW.
OrthoDBiEOG092C5LKY.

Enzyme and pathway databases

BioCyciYEAST:G3O-32172-MONOMER.
BRENDAi1.6.5.2. 984.

Miscellaneous databases

EvolutionaryTraceiQ07923.
PROiQ07923.

Family and domain databases

Gene3Di3.40.50.360. 1 hit.
InterProiIPR029039. Flavoprotein-like_dom.
IPR005025. FMN_Rdtase-like.
[Graphical view]
PfamiPF03358. FMN_red. 1 hit.
[Graphical view]
SUPFAMiSSF52218. SSF52218. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLOT6_YEAST
AccessioniPrimary (citable) accession number: Q07923
Secondary accession number(s): D6VY13
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1270 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.