Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

NAD(P)H-dependent FMN reductase LOT6

Gene

LOT6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has several reductase activities that are NAD(P)H-dependent and involve FMN as a cofactor, ferricyanide being the best substrate for reduction. May be involved in ferric iron assimilation.1 Publication

Miscellaneous

Present with 1270 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

FMNH2 + NAD(P)+ = FMN + NAD(P)H.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei11FMN1
Binding sitei124FMN1

GO - Molecular functioni

  • FMN binding Source: GO_Central
  • FMN reductase (NADH) activity Source: UniProtKB-EC
  • FMN reductase (NADPH) activity Source: GO_Central
  • NAD(P)H dehydrogenase (quinone) activity Source: SGD
  • transcription factor binding Source: SGD

GO - Biological processi

  • apoptotic process Source: SGD
  • cellular response to oxidative stress Source: SGD
  • cytoplasmic sequestering of transcription factor Source: SGD

Keywordsi

Molecular functionOxidoreductase
LigandFlavoprotein, FMN, NAD, NADP

Enzyme and pathway databases

BioCyciYEAST:G3O-32172-MONOMER
BRENDAi1.6.5.2 984

Names & Taxonomyi

Protein namesi
Recommended name:
NAD(P)H-dependent FMN reductase LOT6 (EC:1.5.1.39)
Short name:
FMN reductase LOT6
Alternative name(s):
Azoreductase LOT6
FMN reductase [NAD(P)H]
Low temperature response protein 6
Gene namesi
Name:LOT6
Ordered Locus Names:YLR011W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR011W
SGDiS000004001 LOT6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002346611 – 191NAD(P)H-dependent FMN reductase LOT6Add BLAST191

Proteomic databases

MaxQBiQ07923
PaxDbiQ07923
PRIDEiQ07923

Expressioni

Inductioni

Induced by low temperature and by cycloheximide.1 Publication

Interactioni

Subunit structurei

Homodimer.1 Publication

GO - Molecular functioni

  • transcription factor binding Source: SGD

Protein-protein interaction databases

BioGridi31285, 62 interactors
DIPiDIP-4604N
IntActiQ07923, 1 interactor
MINTiQ07923
STRINGi4932.YLR011W

Structurei

Secondary structure

1191
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 7Combined sources6
Helixi16 – 28Combined sources13
Turni31 – 37Combined sources7
Beta strandi39 – 43Combined sources5
Helixi45 – 48Combined sources4
Helixi61 – 63Combined sources3
Helixi67 – 69Combined sources3
Helixi73 – 83Combined sources11
Beta strandi86 – 93Combined sources8
Helixi101 – 108Combined sources8
Turni112 – 116Combined sources5
Beta strandi118 – 125Combined sources8
Turni126 – 129Combined sources4
Helixi130 – 142Combined sources13
Beta strandi146 – 154Combined sources9
Turni164 – 166Combined sources3
Helixi168 – 173Combined sources6
Helixi174 – 184Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1T0IX-ray2.00A/B1-191[»]
ProteinModelPortaliQ07923
SMRiQ07923
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ07923

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni94 – 97FMN binding4

Phylogenomic databases

HOGENOMiHOG000263121
InParanoidiQ07923
OMAiIFVTPQY
OrthoDBiEOG092C5LKY

Family and domain databases

Gene3Di3.40.50.360, 1 hit
InterProiView protein in InterPro
IPR029039 Flavoprotein-like_sf
IPR005025 FMN_Rdtase-like
PfamiView protein in Pfam
PF03358 FMN_red, 1 hit
SUPFAMiSSF52218 SSF52218, 1 hit

Sequencei

Sequence statusi: Complete.

Q07923-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVGIIMGSV RAKRVCPEIA AYVKRTIENS EELIDQKLKI QVVDLQQIAL
60 70 80 90 100
PLYEDDDELI PAQIKSVDEY ADSKTRSWSR IVNALDIIVF VTPQYNWGYP
110 120 130 140 150
AALKNAIDRL YHEWHGKPAL VVSYGGHGGS KCNDQLQEVL HGLKMNVIGG
160 170 180 190
VAVKIPVGTI PLPEDIVPQL SVHNEEILQL LASCIETTRN K
Length:191
Mass (Da):21,281
Last modified:November 1, 1996 - v1
Checksum:i90C8FD4A39BA2FB7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73183 Genomic DNA Translation: CAA97533.1
AY558199 Genomic DNA Translation: AAS56525.1
BK006945 Genomic DNA Translation: DAA09329.1
PIRiS64833
RefSeqiNP_013111.1, NM_001181898.1

Genome annotation databases

EnsemblFungiiYLR011W; YLR011W; YLR011W
GeneIDi850698
KEGGisce:YLR011W

Similar proteinsi

Entry informationi

Entry nameiLOT6_YEAST
AccessioniPrimary (citable) accession number: Q07923
Secondary accession number(s): D6VY13
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health