Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nuclear receptor subfamily 4 group A member 2

Gene

Nr4a2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons (By similarity). May confer liver-specific regulation of delayed-early genes induced later in the G1 phase of regeneration along with HMR.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi260 – 33576Nuclear receptorPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri263 – 28321NR C4-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri299 – 32325NR C4-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: RGD
  • protein heterodimerization activity Source: UniProtKB
  • RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: NTNU_SB
  • RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding Source: RGD
  • sequence-specific DNA binding Source: MGI
  • steroid hormone receptor activity Source: InterPro
  • transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding Source: NTNU_SB
  • transcription factor activity, sequence-specific DNA binding Source: RGD
  • zinc ion binding Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear receptor subfamily 4 group A member 2
Alternative name(s):
NUR-related factor 1
Nuclear orphan receptor HZF-3
Orphan nuclear receptor NURR1
Regenerating liver nuclear receptor 1
Short name:
RNR-1
SL-322
Gene namesi
Name:Nr4a2
Synonyms:Hzf-3, Nurr1, Rnr1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3202. Nr4a2.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus

  • Note: Mostly nuclear; oxidative stress promotes cytoplasmic localization.By similarity

GO - Cellular componenti

  • cytoplasm Source: RGD
  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 598598Nuclear receptor subfamily 4 group A member 2PRO_0000053720Add
BLAST

Proteomic databases

PaxDbiQ07917.
PRIDEiQ07917.

PTM databases

iPTMnetiQ07917.
PhosphoSiteiQ07917.

Expressioni

Tissue specificityi

Shows a ubiquitous distribution in the cerebral cortex, hippocampus, thalamus, amygdala, and midbrain. Expression increases in prenatally stressed adult offspring in the ventral tegmental area, whereas no changes are observed in the substantia nigra area (at protein level). Not expressed in quiescent liver but is rapidly induced following partial hepatectomy and is specific to hepatic growth as it is not induced in other mitogen-treated cells. Expressed at very low levels in the lung, spleen and stomach and at high levels in the brain.1 Publication

Interactioni

Subunit structurei

Interacts with SFPQ, NCOR2, SIN3A and HADC1. The interaction with NCOR2 increases in the absence of PITX3. Interacts with PER2 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ07917. 1 interaction.
STRINGi10116.ENSRNOP00000043812.

Structurei

3D structure databases

ProteinModelPortaliQ07917.
SMRiQ07917. Positions 261-598.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni409 – 45951Ligand-bindingSequence analysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi287 – 31428Bipartite nuclear localization signal (NLS1)By similarityAdd
BLAST
Motifi338 – 35013Nuclear localization signal (NLS1)By similarityAdd
BLAST
Motifi443 – 45210nuclear export sequence (NES1)By similarity
Motifi568 – 57710nuclear export sequence (NES2)By similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi80 – 13859Gln-richAdd
BLAST
Compositional biasi127 – 233107Pro-richAdd
BLAST
Compositional biasi346 – 39146Pro-richAdd
BLAST

Domaini

the ligand-binding domain (LBD) contains no cavity as a result of the tight packing of side chains from several bulky hydrophobic residues in the region normally occupied by ligands. NR4A2 lacks a 'classical' binding site for coactivators (By similarity).By similarity

Sequence similaritiesi

Contains 1 nuclear receptor DNA-binding domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri263 – 28321NR C4-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri299 – 32325NR C4-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG4217. Eukaryota.
ENOG410YWNC. LUCA.
HOGENOMiHOG000230925.
HOVERGENiHBG052663.
InParanoidiQ07917.
KOiK08558.
OrthoDBiEOG75XGKJ.
PhylomeDBiQ07917.
TreeFamiTF315430.

Family and domain databases

Gene3Di1.10.565.10. 1 hit.
3.30.50.10. 1 hit.
InterProiIPR003070. Nuc_orph_rcpt.
IPR000536. Nucl_hrmn_rcpt_lig-bd.
IPR001723. Nuclear_hrmn_rcpt.
IPR003073. NURR_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamiPF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PRINTSiPR01284. NUCLEARECPTR.
PR01287. NURRNUCRCPTR.
PR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTiSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 1 hit.
PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q07917-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPCVQAQYGS SPQGASPASQ SYSYHSSGEY SSDFLTPEFV KFSMDLTNTE
60 70 80 90 100
ITATTSLPSF STFMDNYSTG YDVKPPCLYQ MPLSGQQSSI KVEDIQMHNY
110 120 130 140 150
QQHSHLPPQS EEMMPHSGSV YYKPSSPPTP STPGFQVQHS PMWDDPGSLH
160 170 180 190 200
NFHQNYVATT HMIEQRKTPV SRLSLFSFKQ SRPGTPVSSC QMRFDGPLHV
210 220 230 240 250
PMNPEPAGSH HVVDGQTFAV PNPIRKPASM GFPGLQIGHA SQLLDTQVPS
260 270 280 290 300
PPSRGSPSNE GLCAVCGDNA ACQHYGVRTC EGCKGFFKRT VQKNAKYVCL
310 320 330 340 350
ANKNCPVDKR RRNRCQYCRF QKCLAVGMVK EVVRTDSLKG RRGRLPSKPK
360 370 380 390 400
SPQDPSPPSP PVSLISALVR AHVDSNPAMT SLDYSRFQAN PDYQMSGDDT
410 420 430 440 450
QHIQQFYDLL TGSMEIIRGW AEKIPGFADL PKADQDLLFE SAFLELFVLR
460 470 480 490 500
LAYRSNPVEG KLIFCNGVVL HRLQCVRGFG EWIDSIVEFS SNLQNMNIDI
510 520 530 540 550
SAFSCIAALA MVTERHGLKE PKRVEELQNK IVNCLKDHVT FNNGGLNRPN
560 570 580 590
YLSKLLGKLP ELRTLCTQGL QRIFYLKLED LVPPPAIIDK LFLDTLPF
Length:598
Mass (Da):66,622
Last modified:July 15, 1999 - v3
Checksum:i0A54ED59FB70DEA8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti182 – 1821R → A in AAA42058 (PubMed:8473329).Curated
Sequence conflicti182 – 1821R → P in AAC53315 (PubMed:9221923).Curated
Sequence conflicti250 – 2501S → P in AAA42058 (PubMed:8473329).Curated
Sequence conflicti252 – 2521P → A in AAC52143 (PubMed:7914660).Curated
Sequence conflicti362 – 3621V → G in AAA42058 (PubMed:8473329).Curated
Sequence conflicti364 – 3718LISALVRA → DQCPRQT in AAA42058 (PubMed:8473329).Curated
Sequence conflicti434 – 4341D → S in AAA42058 (PubMed:8473329).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01146 mRNA. Translation: AAC52143.1.
L08595 mRNA. Translation: AAA42058.1.
U72345 mRNA. Translation: AAC53315.1.
PIRiI84692.
RefSeqiNP_062201.2. NM_019328.3.
XP_006234263.1. XM_006234201.2.
UniGeneiRn.88129.

Genome annotation databases

GeneIDi54278.
KEGGirno:54278.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01146 mRNA. Translation: AAC52143.1.
L08595 mRNA. Translation: AAA42058.1.
U72345 mRNA. Translation: AAC53315.1.
PIRiI84692.
RefSeqiNP_062201.2. NM_019328.3.
XP_006234263.1. XM_006234201.2.
UniGeneiRn.88129.

3D structure databases

ProteinModelPortaliQ07917.
SMRiQ07917. Positions 261-598.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ07917. 1 interaction.
STRINGi10116.ENSRNOP00000043812.

PTM databases

iPTMnetiQ07917.
PhosphoSiteiQ07917.

Proteomic databases

PaxDbiQ07917.
PRIDEiQ07917.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi54278.
KEGGirno:54278.

Organism-specific databases

CTDi4929.
RGDi3202. Nr4a2.

Phylogenomic databases

eggNOGiKOG4217. Eukaryota.
ENOG410YWNC. LUCA.
HOGENOMiHOG000230925.
HOVERGENiHBG052663.
InParanoidiQ07917.
KOiK08558.
OrthoDBiEOG75XGKJ.
PhylomeDBiQ07917.
TreeFamiTF315430.

Miscellaneous databases

PROiQ07917.

Family and domain databases

Gene3Di1.10.565.10. 1 hit.
3.30.50.10. 1 hit.
InterProiIPR003070. Nuc_orph_rcpt.
IPR000536. Nucl_hrmn_rcpt_lig-bd.
IPR001723. Nuclear_hrmn_rcpt.
IPR003073. NURR_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamiPF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PRINTSiPR01284. NUCLEARECPTR.
PR01287. NURRNUCRCPTR.
PR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTiSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 1 hit.
PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Expression of nuclear hormone receptors within the rat hippocampus: identification of novel orphan receptors."
    Pena de Ortiz S., Cannon M.M., Jamieson G.A. Jr.
    Brain Res. Mol. Brain Res. 23:278-283(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Hippocampus.
  2. "RNR-1, a nuclear receptor in the NGFI-B/Nur77 family that is rapidly induced in regenerating liver."
    Scearce L.M., Laz T.M., Hazel T.G., Lau L.F., Taub R.
    J. Biol. Chem. 268:8855-8861(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  3. "Rat nurr1 is prominently expressed in perirhinal cortex, and differentially induced in the hippocampal dentate gyrus by electroconvulsive vs. kindled seizures."
    Xing G.Q., Zhang L.X., Zhang L., Heynen T., Li X.L., Smith M.A., Weiss S.R.B., Feldman A.N., Detera-Wadleigh S., Chuang D.M., Post R.M.
    Brain Res. Mol. Brain Res. 47:251-261(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.
  4. "Immunocytochemical expression of dopamine-related transcription factors Pitx3 and Nurr1 in prenatally stressed adult rats."
    Katunar M.R., Saez T., Brusco A., Antonelli M.C.
    J. Neurosci. Res. 87:1014-1022(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiNR4A2_RAT
AccessioniPrimary (citable) accession number: Q07917
Secondary accession number(s): O35865
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: July 15, 1999
Last modified: July 6, 2016
This is version 137 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.