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Protein

POU domain, class 6, transcription factor 1

Gene

Pou6f1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that binds preferentially to a variant of the octamer motif (5'-ATGATAAT-3').By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi234 – 293HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

  • DNA binding Source: MGI
  • sequence-specific DNA binding Source: MGI
  • transcription factor activity, sequence-specific DNA binding Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain, class 6, transcription factor 1
Alternative name(s):
Octamer-binding transcription factor EMB
Transcription regulatory protein MCP-1
Gene namesi
Name:Pou6f1
Synonyms:Emb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:102935. Pou6f1.

Subcellular locationi

GO - Cellular componenti

  • actin cytoskeleton Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001007601 – 301POU domain, class 6, transcription factor 1Add BLAST301

Proteomic databases

PaxDbiQ07916.
PRIDEiQ07916.

PTM databases

iPTMnetiQ07916.
PhosphoSitePlusiQ07916.

Expressioni

Tissue specificityi

Isoform C1 and isoform C2 are found in the brain, while isoform C7 is found in the testis.

Developmental stagei

Expressed in the embryo throughout post-implantation stages with the most prominent expression in the developing CNS. In the adult, it is highly expressed in brain whereas weaker expression can be detected in other organs.

Gene expression databases

BgeeiENSMUSG00000009739.
CleanExiMM_EMB.
MM_POU6F1.
ExpressionAtlasiQ07916. baseline and differential.
GenevisibleiQ07916. MM.

Interactioni

Protein-protein interaction databases

BioGridi202314. 4 interactors.
STRINGi10090.ENSMUSP00000134760.

Structurei

3D structure databases

ProteinModelPortaliQ07916.
SMRiQ07916.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini139 – 213POU-specificPROSITE-ProRule annotationAdd BLAST75

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1 – 139Gln/Pro-richAdd BLAST139

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
Contains 1 POU-specific domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG3802. Eukaryota.
ENOG410XQ7X. LUCA.
GeneTreeiENSGT00760000118935.
HOGENOMiHOG000115597.
HOVERGENiHBG008230.
InParanoidiQ07916.
OrthoDBiEOG091G04YJ.
PhylomeDBiQ07916.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_dom.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform C2 (identifier: Q07916-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPGISSQILT NAQGQVIGAL PWVVNSASVA TPAPAQSLQV QAVTPQLLLN
60 70 80 90 100
AQGQVIATLA SSPLPQPVAV RKPNTPESPA KSEVQPIQPT QAVPQPAVIL
110 120 130 140 150
TSPTPALKPS AATPIPITCS ETPTVSQLVS KPHTPSLDED GINLEEIREF
160 170 180 190 200
AKNFKIRRLS LGLTQTQVGQ ALTATEGPAY SQSAICRFEK LDITPKSAQK
210 220 230 240 250
LKPVLEKWLM EAELRNQEGQ QNLMEFVGGE PSKKRKRRTS FTPQAIEALN
260 270 280 290 300
AYFEKNPLPT GQEITEIAKE LNYDREVVRV WFCNRRQTLK NTSKLNVFQI

P
Length:301
Mass (Da):32,852
Last modified:November 1, 1996 - v1
Checksum:i9FC71EE845982AD4
GO
Isoform C1 (identifier: Q07916-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     131-131: K → SLE

Show »
Length:303
Mass (Da):33,053
Checksum:i847408491C9C9CAD
GO
Isoform C7 (identifier: Q07916-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-131: MPGISSQILT...TPTVSQLVSK → MCQHTLIFMHVSLVPPSGLE

Show »
Length:190
Mass (Da):21,772
Checksum:i0F170B4F62CF99F4
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0023351 – 131MPGIS…QLVSK → MCQHTLIFMHVSLVPPSGLE in isoform C7. 1 PublicationAdd BLAST131
Alternative sequenceiVSP_002334131K → SLE in isoform C1. Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13801 mRNA. Translation: BAA02959.1.
L13763 mRNA. Translation: AAA53044.1.
AK006732 mRNA. Translation: BAB24718.1.
BC059830 mRNA. Translation: AAH59830.1.
CCDSiCCDS57009.1. [Q07916-1]
PIRiB46078.
I56543.
RefSeqiNP_034257.1. NM_010127.3. [Q07916-1]
XP_011243814.1. XM_011245512.2. [Q07916-1]
XP_011243815.1. XM_011245513.2. [Q07916-1]
UniGeneiMm.28825.

Genome annotation databases

EnsembliENSMUST00000176271; ENSMUSP00000145707; ENSMUSG00000009739. [Q07916-1]
GeneIDi19009.
UCSCiuc007xro.1. mouse. [Q07916-3]
uc007xrp.1. mouse. [Q07916-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13801 mRNA. Translation: BAA02959.1.
L13763 mRNA. Translation: AAA53044.1.
AK006732 mRNA. Translation: BAB24718.1.
BC059830 mRNA. Translation: AAH59830.1.
CCDSiCCDS57009.1. [Q07916-1]
PIRiB46078.
I56543.
RefSeqiNP_034257.1. NM_010127.3. [Q07916-1]
XP_011243814.1. XM_011245512.2. [Q07916-1]
XP_011243815.1. XM_011245513.2. [Q07916-1]
UniGeneiMm.28825.

3D structure databases

ProteinModelPortaliQ07916.
SMRiQ07916.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202314. 4 interactors.
STRINGi10090.ENSMUSP00000134760.

PTM databases

iPTMnetiQ07916.
PhosphoSitePlusiQ07916.

Proteomic databases

PaxDbiQ07916.
PRIDEiQ07916.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000176271; ENSMUSP00000145707; ENSMUSG00000009739. [Q07916-1]
GeneIDi19009.
UCSCiuc007xro.1. mouse. [Q07916-3]
uc007xrp.1. mouse. [Q07916-1]

Organism-specific databases

CTDi5463.
MGIiMGI:102935. Pou6f1.

Phylogenomic databases

eggNOGiKOG3802. Eukaryota.
ENOG410XQ7X. LUCA.
GeneTreeiENSGT00760000118935.
HOGENOMiHOG000115597.
HOVERGENiHBG008230.
InParanoidiQ07916.
OrthoDBiEOG091G04YJ.
PhylomeDBiQ07916.

Miscellaneous databases

ChiTaRSiPou6f1. mouse.
PROiQ07916.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000009739.
CleanExiMM_EMB.
MM_POU6F1.
ExpressionAtlasiQ07916. baseline and differential.
GenevisibleiQ07916. MM.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_dom.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPO6F1_MOUSE
AccessioniPrimary (citable) accession number: Q07916
Secondary accession number(s): Q91XK3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 150 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.