Reviewed, UniProtKB/Swiss-Prot Q07916 (PO6F1_MOUSE)

Names and origin

Protein names

Recommended name:
    POU domain, class 6, transcription factor 1
Alternative name(s):
    Octamer-binding transcription factor EMB
    Transcription regulatory protein MCP-1

Gene names

Name:	Pou6f1
Synonyms:	Emb

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	301 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Transcription factor that binds preferentially to a variant of the octamer motif (5'-ATGATAAT-3') By similarity.
Subcellular location	Nucleus.
Tissue specificity	Isoform C1 and isoform C2 are found in the brain, while isoform C7 is found in the testis.
Developmental stage	Expressed in the embryo throughout post-implantation stages with the most prominent expression in the developing CNS. In the adult, it is highly expressed in brain whereas weaker expression can be detected in other organs.
Sequence similarities	Belongs to the POU transcription factor family. Class-6 subfamily. Contains 1 homeobox DNA-binding domain. Contains 1 POU-specific domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Homeobox
Ligand	DNA-binding
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from direct assay. Source: MGI
Molecular function	sequence-specific DNA binding Ref.1 Inferred from direct assay. Source: MGI transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform C2 (identifier: Q07916-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform C1 (identifier: Q07916-2)

The sequence of this isoform differs from the canonical sequence as follows:
     131-131: K → SLE

Isoform C7 (identifier: Q07916-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-131: MPGISSQILT...TPTVSQLVSK → MCQHTLIFMHVSLVPPSGLE

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 301	301	POU domain, class 6, transcription factor 1		PRO_0000100760
Regions
	Domain	139 – 213	75	POU-specific
	DNA binding	234 – 293	60	Homeobox
	Compositional bias	1 – 139	139	Gln/Pro-rich
Natural variations
	Alternative sequence	1 – 131	131	MPGIS…QLVSK → MCQHTLIFMHVSLVPPSGLE in isoform C7.		VSP_002335
	Alternative sequence	131	1	K → SLE in isoform C1.		VSP_002334

Sequences

	Sequence		Length	Mass (Da)
	Isoform C2 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 9FC71EE845982AD4	FASTA	301	32,852
	10 20 30 40 50 60 MPGISSQILT NAQGQVIGAL PWVVNSASVA TPAPAQSLQV QAVTPQLLLN AQGQVIATLA 70 80 90 100 110 120 SSPLPQPVAV RKPNTPESPA KSEVQPIQPT QAVPQPAVIL TSPTPALKPS AATPIPITCS 130 140 150 160 170 180 ETPTVSQLVS KPHTPSLDED GINLEEIREF AKNFKIRRLS LGLTQTQVGQ ALTATEGPAY 190 200 210 220 230 240 SQSAICRFEK LDITPKSAQK LKPVLEKWLM EAELRNQEGQ QNLMEFVGGE PSKKRKRRTS 250 260 270 280 290 300 FTPQAIEALN AYFEKNPLPT GQEITEIAKE LNYDREVVRV WFCNRRQTLK NTSKLNVFQI P
	Isoform C1. Checksum: 847408491C9C9CAD	FASTA	303	33,053
	10 20 30 40 50 60 MPGISSQILT NAQGQVIGAL PWVVNSASVA TPAPAQSLQV QAVTPQLLLN AQGQVIATLA 70 80 90 100 110 120 SSPLPQPVAV RKPNTPESPA KSEVQPIQPT QAVPQPAVIL TSPTPALKPS AATPIPITCS 130 140 150 160 170 180 ETPTVSQLVS SLEPHTPSLD EDGINLEEIR EFAKNFKIRR LSLGLTQTQV GQALTATEGP 190 200 210 220 230 240 AYSQSAICRF EKLDITPKSA QKLKPVLEKW LMEAELRNQE GQQNLMEFVG GEPSKKRKRR 250 260 270 280 290 300 TSFTPQAIEA LNAYFEKNPL PTGQEITEIA KELNYDREVV RVWFCNRRQT LKNTSKLNVF QIP
	Isoform C7. Checksum: 0F170B4F62CF99F4	FASTA	190	21,772
	10 20 30 40 50 60 MCQHTLIFMH VSLVPPSGLE PHTPSLDEDG INLEEIREFA KNFKIRRLSL GLTQTQVGQA 70 80 90 100 110 120 LTATEGPAYS QSAICRFEKL DITPKSAQKL KPVLEKWLME AELRNQEGQQ NLMEFVGGEP 130 140 150 160 170 180 SKKRKRRTSF TPQAIEALNA YFEKNPLPTG QEITEIAKEL NYDREVVRVW FCNRRQTLKN 190 TSKLNVFQIP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel class of murine POU gene predominantly expressed in central nervous system." Okamoto K., Wakamiya M., Noji S., Koyama E., Taniguchi S., Takemura R., Copeland N.G., Gilbert D.J., Jenkins N.A., Muramatsu M., Hamada H. J. Biol. Chem. 268:7449-7457(1993) [PubMed: 8463278] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Brain and Testis.
[2]	"NMDA receptor activation in differentiating cerebellar cell cultures regulates the expression of a new POU gene, Cns-1." Bulleit R.F., Cui H., Wang J., Lin X. J. Neurosci. 14:1584-1595(1994) [PubMed: 7907365] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: CD-1. Tissue: Brain.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM C7). Strain: C57BL/6J. Tissue: Testis.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM C2). Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D13801 mRNA. Translation: BAA02959.1. L13763 mRNA. Translation: AAA53044.1. AK006732 mRNA. Translation: BAB24718.1. BC059830 mRNA. Translation: AAH59830.1.
IPI	IPI00378160. IPI00474678. IPI00619985.
PIR	B46078. I56543.
RefSeq	NP_034257.1.
UniGene	Mm.28825
3D structure databases
HSSP	HSSP built from PDB template 1CQT based on UniProtKB P14859.
ModBase	Search...
PTM databases
PhosphoSite	Q07916.
Genome annotation databases
Ensembl	ENSMUSG00000009739. Mus musculus. [Contig view]
GeneID	19009.
KEGG	mmu:19009.
Organism-specific databases
MGI	MGI:102935. Pou6f1.
Phylogenomic databases
HOVERGEN	Q07916.
Gene expression databases
ArrayExpress	Q07916.
Bgee	Q07916.
CleanEx	MM_EMB. MM_POU6F1.
GermOnline	ENSMUSG00000009739. Mus musculus.
Family and domain databases
InterPro	IPR001356. Homeobox. IPR017970. Homeobox_CS. IPR012287. Homeodomain-rel. IPR013847. POU. IPR000327. POU_specific. [Graphical view]
Gene3D	G3DSA:1.10.10.60. Homeodomain-rel. 1 hit.
Pfam	PF00046. Homeobox. 1 hit. PF00157. Pou. 1 hit. [Graphical view]
PRINTS	PR00028. POUDOMAIN.
ProDom	PD000010. Homeobox. 1 hit. PD000583. POU. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00389. HOX. 1 hit. SM00352. POU. 1 hit. [Graphical view]
PROSITE	PS00027. HOMEOBOX_1. False negative. PS50071. HOMEOBOX_2. 1 hit. PS00035. POU_1. 1 hit. PS00465. POU_2. 1 hit. PS51179. POU_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	295414.
SOURCE	Search...

Entry information

Entry name

PO6F1_MOUSE

Accession

Primary (citable) accession number: Q07916
Secondary accession number(s): Q91XK3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)