Q07896 (NOC3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleolar complex-associated protein 3

Gene names

Name:	NOC3
Ordered Locus Names:	YLR002C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	663 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for synthesis of 60S ribosomal subunits and the transport of pre-ribosomes from the nucleoplasm to the cytoplasm. Also required for initiation of DNA replication. May function downstream of the origin recognition complex (ORC complex) in the loading of CDC6 and the minichromosome maintenance complex (MCM complex) onto chromatin during the G1 phase of the cell cycle. Essential for growth. Ref.4 Ref.6
Subunit structure	Forms a heterodimer with NOC2. This complex may be associated with pre-ribosomal particles. Also interacts with MCM2, MCM5 and ORC1. Ref.4 Ref.5 Ref.6
Subcellular location	Nucleus › nucleolus Ref.4.
Miscellaneous	Present with 11700 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the CBF/MAK21 family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA replication Ribosome biogenesis
Cellular component	Nucleus
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication initiation Inferred from direct assay Ref.6. Source: SGD cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW pre-replicative complex assembly Inferred from mutant phenotype PubMed 22421151. Source: SGD rRNA processing Inferred from direct assay Ref.6. Source: SGD
Cellular_component	Noc2p-Noc3p complex Inferred from physical interaction Ref.4. Source: SGD nucleolus Inferred from direct assay Ref.4. Source: SGD pre-replicative complex Inferred from direct assay PubMed 22421151. Source: SGD
Molecular_function	chromatin binding Inferred from direct assay Ref.6. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
NOC2	P39744	3	EBI-36093,EBI-29259

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 663	663	Nucleolar complex-associated protein 3		PRO_0000173483
Regions
	Coiled coil	363 – 408	46	Potential
Amino acid modifications
	Modified residue	49	1	Phosphoserine Ref.8
	Modified residue	50	1	Phosphoserine Ref.8
	Modified residue	395	1	Phosphoserine Ref.8

Sequences

	Sequence		Length	Mass (Da)
	Q07896 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 4CA9461A4A1F1E9E	FASTA	663	75,582
	10 20 30 40 50 60 MAKRNRSQFR IQERTAKKRK HEDSLLEGNV FQNAPEDMDE NTIYSAKGSS WDEEEQDYEM 70 80 90 100 110 120 VPRKNRSDTS NLVEGLPIKV NGKVERKLHK AQEKPKDDDE EDEDSNDSSE DDEGPNEEQE 130 140 150 160 170 180 AEAKEDEPDT EEKILQLKED IADLVTKVME EPEENTAALG RLCKMVESKN PNTCKFSMLA 190 200 210 220 230 240 LVPVFKSIIP GYRIRPLTET EKKEKVSKEV SKLRNFEQAL VYNYKNYVGR LQSLSKTPSN 250 260 270 280 290 300 AAPIQVSLGI LATQAAKELI STASHFNFRT DIFTLLLRRI CKPRISTDPT SIQIIQTFET 310 320 330 340 350 360 LLNEDEEGSI SFEILRIFNK ILKTRNFNIE ESVLNMLLSL DVLHDYDPNT KLKGNVSAPK 370 380 390 400 410 420 LKKKDRVHLS KKQRKARKEM QQIEEEMRNA EQAVSAEERE RNQSEILKIV FTIYLNILKN 430 440 450 460 470 480 NAKTLIGSVL EGLTKFGNMA NFDLLGDFLE VMKELISDTE FDNLSSAEVR KALLCIVSAF 490 500 510 520 530 540 SLISNTQYMK VNVDLSKFVD GLYALLPYIC LDADIELSYR SLRLADPLNN EIIKPSVNVS 550 560 570 580 590 600 TKAELLLKAL DHVFFRSKSG TKERATAFTK RLYMCISHTP EKTSIAILKF IDKLMNRYPE 610 620 630 640 650 660 ISGLYSSEDR IGNGHFIMEA DNPSRSNPEA ATLWDNALLE KHYCPVVTKG LRSLSSRSKE CSK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Sequence analysis of the CEN12 region of Saccharomyces cerevisiae on a 43.7 kb fragment of chromosome XII including an open reading frame homologous to the human cystic fibrosis transmembrane conductance regulator protein CFTR." Miosga T., Zimmermann F.K. Yeast 12:693-708(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 443-663. Strain: ATCC 90840 / EAY235 / FY23.
[4]	"Maturation and intranuclear transport of pre-ribosomes requires Noc proteins." Milkereit P., Gadal O., Podtelejnikov A., Trumtel S., Gas N., Petfalski E., Tollervey D., Mann M., Hurt E., Tschochner H. Cell 105:499-509(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH NOC2 AND 66S PRE-RIBOSOMAL PARTICLES.
[5]	"Identification of a 60S preribosomal particle that is closely linked to nuclear export." Bassler J., Grandi P., Gadal O., Lessmann T., Petfalski E., Tollervey D., Lechner J., Hurt E. Mol. Cell 8:517-529(2001) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN 60S PRE-RIBOSOMAL PARTICLES.
[6]	"Noc3p, a bHLH protein, plays an integral role in the initiation of DNA replication in budding yeast." Zhang Y., Yu Z., Fu X., Liang C. Cell 109:849-860(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH MCM2; MCM5 AND ORC1.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-49; SER-50 AND SER-395, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z73174 Genomic DNA. Translation: CAA97524.1. X91488 Genomic DNA. Translation: CAA62771.1. BK006945 Genomic DNA. Translation: DAA09320.1.
PIR	S64824.
RefSeq	NP_013102.1. NM_001181889.1.
3D structure databases
ProteinModelPortal	Q07896.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4839N.
IntAct	Q07896. 19 interactions.
MINT	MINT-490945.
STRING	4932.YLR002C.
Proteomic databases
PaxDb	Q07896.
PeptideAtlas	Q07896.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR002C; YLR002C; YLR002C.
GeneID	850688.
KEGG	sce:YLR002C.
Organism-specific databases
CYGD	YLR002c.
SGD	S000003992. NOC3.
Phylogenomic databases
eggNOG	COG5117.
GeneTree	ENSGT00390000008540.
HOGENOM	HOG000168453.
KO	K14834.
OMA	TREKPVT.
OrthoDB	EOG4R53B6.
Gene expression databases
Genevestigator	Q07896.
GermOnline	YLR002C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005612. CCAAT-binding_factor. IPR011501. NOC3p. IPR016903. Nucleolar_cplx-assoc_3. [Graphical view]
Pfam	PF03914. CBF. 1 hit. PF07540. NOC3p. 1 hit. [Graphical view]
PIRSF	PIRSF028977. Nucleolar_complex_p3. 1 hit.
ProtoNet	Search...
Other
NextBio	966701.

Entry information

Entry name

NOC3_YEAST

Accession

Primary (citable) accession number: Q07896
Secondary accession number(s): D6VY04, Q92280

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2005
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program